BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,723,067	1 bp→AT	intergenic (‑181/‑120)	U712_19190 ← / → U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,723,066	1	.	A	97.8%	473.4 / 1.9	134	intergenic (‑180/‑121)	U712_19190/U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
Reads supporting (aligned to +/- strand): ref base . (2/1); new base A (51/80); total (53/81)
Fisher's exact test for biased strand distribution p-value = 5.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01
*	CP006881	3,723,067	0	G	T	97.7%	447.6 / ‑1.3	134	intergenic (‑181/‑120)	U712_19190/U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
Reads supporting (aligned to +/- strand): ref base G (1/0); major base T (50/80); minor base A (2/1); total (53/81)
Fisher's exact test for biased strand distribution p-value = 5.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01

Only 100 of 137 total aligned reads displayed.

CGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAACT‑GTTTTT‑TAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAGGAAAAAACAATGGAAACTCAAGCA  >  CP006881/3722935‑3723201

||

cGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACT‑Attttt‑t                                                                                                                                  <  4:80316/139‑1 (MQ=255)

        ggACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTaaa                                                                                                                           <  7:285222/139‑1 (MQ=255)

        ggACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTaaa                                                                                                                           <  1:373102/139‑1 (MQ=255)

         gACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCAGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTaaa                                                                                                                           >  4:73516/1‑138 (MQ=255)

            acaGGGATCCTTTTTCTTTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCAGATTCCCGCTTATTGAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCTAGTATTTTTT‑TTGTTAAATAGt                                                                                                                       <  5:415238/139‑1 (MQ=255)

            acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGt                                                                                                                       >  4:206882/1‑139 (MQ=255)

            acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGt                                                                                                                       <  1:116289/139‑1 (MQ=255)

            acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGt                                                                                                                       <  4:378078/139‑1 (MQ=255)

            acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGt                                                                                                                       <  6:309807/139‑1 (MQ=255)

             caGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGtt                                                                                                                      >  6:239672/1‑139 (MQ=255)

              aGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGttt                                                                                                                     >  1:103782/1‑139 (MQ=255)

              aGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGttt                                                                                                                     >  5:49910/1‑139 (MQ=255)

                cGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGa                                                                                                                   >  5:181573/1‑139 (MQ=255)

                   tCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATat                                                                                                                <  5:242211/139‑2 (MQ=255)

                   tCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATa                                                                                                                 >  8:160376/1‑138 (MQ=255)

                   tCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCAGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATa                                                                                                                 >  5:210683/1‑138 (MQ=255)

                        tttCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAAcat                                                                                                           >  2:310263/1‑137 (MQ=255)

                        tttCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAAcat                                                                                                           >  3:200685/1‑137 (MQ=255)

                        tttCATTTACGCAAATTTTAGGTCTTGCATGCTTTACCAGTCACAATCCCGCTTATTAAGATTAAGAATACGCTTTCATCATAAATCATGATAGCTTTTTCTTCAAGA‑ATTTTTATAGTTAAATATTTTTATATAAcat                                                                                                           >  4:283642/1‑137 (MQ=255)

                                       ttttAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCg                                                                                            <  5:330532/139‑1 (MQ=255)

                                       ttttAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCg                                                                                            <  1:10842/139‑1 (MQ=255)

                                          tAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTa                                                                                          <  3:119039/138‑1 (MQ=255)

                                            ggTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTaaa                                                                                        >  7:302149/1‑138 (MQ=255)

                                                  gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaat                                                                                 >  3:378499/1‑137 (MQ=255)

                                                  gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaa                                                                                  <  1:239295/138‑2 (MQ=255)

                                                  gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaa                                                                                  <  3:22329/138‑2 (MQ=255)

                                                       cTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATtgtg                                                                             <  7:160376/138‑1 (MQ=255)

                                                       cTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATtgtg                                                                             >  5:13671/1‑138 (MQ=255)

                                                       cTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAGTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATtgtg                                                                             >  5:212378/1‑138 (MQ=255)

                                                            ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAATTTAATTGTGaaata                                                                        <  6:384067/138‑3 (MQ=255)

                                                            ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                        <  2:103782/138‑3 (MQ=255)

                                                             cAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACt                                                                      <  5:71411/139‑1 (MQ=255)

                                                              aGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACtt                                                                     <  6:49910/139‑1 (MQ=255)

                                                                tCACAATCCCGCTTATTCAGATTAAGAATACTCTTTCATCATACATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                                   <  6:286548/139‑1 (MQ=255)

                                                                 cacaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTATACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                                   <  6:210683/138‑1 (MQ=255)

                                                                 cacaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                                   >  7:72995/1‑138 (MQ=255)

                                                                  acaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTcaca                                                                 <  2:280049/139‑1 (MQ=255)

                                                                    aaTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATAATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTTTGAAATACTTCACaa                                                                >  8:21431/1‑138 (MQ=255)

                                                                     ctcacgcgTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  8:322865/135‑1 (MQ=255)

                                                                     ataccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  7:264607/136‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGACTACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  6:363742/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  3:332515/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  6:414467/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  5:186329/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  6:303490/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  1:310263/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  4:378683/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  4:378499/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  4:298664/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  8:302149/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  2:42555/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  1:204642/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  3:73516/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  3:283642/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  3:319231/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATAAGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  6:22580/139‑1 (MQ=255)

                                                                      tCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAatat                                                             >  7:465397/1‑139 (MQ=255)

                                                                         cGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGt                                                          >  6:94236/1‑139 (MQ=255)

                                                                          gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  7:44730/134‑1 (MQ=255)

                                                                          gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              >  8:44730/1‑134 (MQ=255)

                                                                          gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                         <  5:94236/139‑1 (MQ=255)

                                                                          gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                         <  4:268852/139‑1 (MQ=255)

                                                                          gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                         <  2:344567/139‑1 (MQ=255)

                                                                          gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                         <  5:239672/139‑1 (MQ=255)

                                                                           cTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              >  5:97195/1‑133 (MQ=255)

                                                                           cTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              >  7:152029/1‑133 (MQ=255)

                                                                           cTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  6:97195/133‑1 (MQ=255)

                                                                           cTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                         >  6:89139/1‑138 (MQ=255)

                                                                               ttCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCtata                                                    <  2:222901/139‑1 (MQ=255)

                                                                               ttCAGATTAAGAATACGCTTTCATCATAAATCAAGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTATATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCtata                                                    <  2:349678/139‑1 (MQ=255)

                                                                                tCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TATTTAAATAGTTTGATATAACATGTATCCAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCtata                                                    >  1:241324/1‑138 (MQ=255)

                                                                                tCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TATTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCtata                                                    <  3:175039/138‑1 (MQ=255)

                                                                                tCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCtata                                                    <  6:379638/138‑1 (MQ=255)

                                                                                    aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCg                                                           <  6:41796/127‑1 (MQ=255)

                                                                                    aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCtata                                                    >  7:423916/1‑134 (MQ=255)

                                                                                    aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCtata                                                    <  8:423916/134‑1 (MQ=255)

                                                                                    aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATg                                               >  1:367566/1‑139 (MQ=255)

                                                                                    aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATg                                               >  5:147870/1‑139 (MQ=255)

                                                                                     ttaagaATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TATTTAAATAGTTTGATATAACATGTATACAAAAATTCGTAAAAATTAATTGTTAAATAATTCACAATATAGTGCTATAatatt                                               >  6:156286/4‑137 (MQ=255)

                                                                                         gaATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCaa                                          >  4:342401/1‑139 (MQ=255)

                                                                                              cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATtgtg                                                                             <  4:179318/99‑1 (MQ=255)

                                                                                              cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATtgtg                                                                             >  3:179318/1‑99 (MQ=255)

                                                                                              cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                                   >  1:318352/1‑109 (MQ=255)

                                                                                              cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCAt                                      >  6:254922/1‑138 (MQ=255)

                                                                                                   tcatcaTAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                        <  3:88107/99‑3 (MQ=255)

                                                                                                    catcatAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                        >  2:47493/1‑96 (MQ=255)

                                                                                                    catcatAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                        <  3:272098/98‑3 (MQ=255)

                                                                                                    catcatAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                        <  1:47493/98‑3 (MQ=255)

                                                                                                    catcatAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaaga                                >  1:324490/1‑138 (MQ=255)

                                                                                                    catcatAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaaga                                >  7:88632/1‑138 (MQ=255)

                                                                                                     atcatAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaagaa                               <  4:209075/138‑1 (MQ=255)

                                                                                                         tAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCt                                                       >  8:316828/1‑110 (MQ=255)

                                                                                                               aTGATAGCGTTTTCGTCAACT‑ATTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGaaaaga                   >  8:221618/1‑137 (MQ=255)

                                                                                                                tGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGaaaaga                   <  6:181573/139‑3 (MQ=255)

                                                                                                                 gATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  5:68727/95‑1 (MQ=255)

                                                                                                                 gATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGaaaaga                   >  1:41940/1‑136 (MQ=255)

                                                                                                                  aTAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGAc                  >  6:117008/1‑138 (MQ=255)

                                                                                                                  aTAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGAc                  >  4:259249/1‑138 (MQ=255)

                                                                                                                      cGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATg              >  5:282850/1‑138 (MQ=255)

                                                                                                                                aactatttttt‑tAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAg    <  6:147870/132‑1 (MQ=255)

||

CGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAACT‑GTTTTT‑TAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAGGAAAAAACAATGGAAACTCAAGCA  >  CP006881/3722935‑3723201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`