Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,578,466 Δ1 bp 100% intergenic (‑59/‑76) U712_18365 ← / → U712_18370 Stage II sporulation protein Q/Putative phosphinothricin acetyltransferase YwnH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,578,4660A.98.5% 302.9 / ‑2.9 68intergenic (‑59/‑76)U712_18365/U712_18370Stage II sporulation protein Q/Putative phosphinothricin acetyltransferase YwnH
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base . (30/37);  total (30/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

TTGCAGGGAACACCCAGCGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCCAAAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACTTGGAGGCTGTTGTGGCGATTTA  >  CP006881/3578329‑3578600
                                                                                                                                         |                                                                                                                                      
ttGCAGGGAACACCCAGCGTTTACGAAAAACCTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCCGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCCa                                                                                                                                        >  8:270172/1‑138 (MQ=255)
ttGCAGGGAACACCCAGCGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCCa                                                                                                                                        >  7:140110/1‑138 (MQ=255)
ttGCAGGGAACACCCAGCGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCCa                                                                                                                                        >  6:391090/1‑138 (MQ=255)
ttGCAGGGAACACCCAGCGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCCa                                                                                                                                        >  7:96363/1‑138 (MQ=255)
               aGCGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGAt                                                                                                                       <  5:23771/139‑1 (MQ=255)
               aGCGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGAt                                                                                                                       <  5:391090/139‑1 (MQ=255)
               aGCGTTTACGAAAAAACTGCGGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGAt                                                                                                                       <  4:1614/139‑1 (MQ=255)
                   tttACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAg                                                                                                                   <  3:354328/139‑1 (MQ=255)
                     tACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACCACCTATCC‑AAAAAACTTTTTTAGATGTAGtt                                                                                                                 >  4:397823/1‑139 (MQ=255)
                         aaaactctgctgAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGca                                                                                                             <  7:11934/133‑1 (MQ=255)
                          aaaaaCTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGca                                                                                                             <  1:302664/138‑1 (MQ=255)
                                                cTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTAtt                                                                                      <  8:18618/139‑1 (MQ=255)
                                                cTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTAtt                                                                                      <  8:96363/139‑1 (MQ=255)
                                                cTTGAGAAGTTTTCATTTCTTCCTCTCTCATTGTGTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAAAATATACAACCTATCC‑AAAAAACTGTTTTAGCTGTAGTTTGCACAGACTGCACGAAAATATGTAtt                                                                                      <  8:324220/139‑1 (MQ=255)
                                                   gagaAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTAT‑CAAAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTAtt                                                                                      <  8:4986/136‑1 (MQ=255)
                                                         ttttcttttcTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTgaga                                                                             <  5:319325/139‑1 (MQ=255)
                                                         ttttcttttcTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTgaga                                                                             <  7:30432/139‑1 (MQ=255)
                                                             cttttcttCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAgagga                                                                          <  2:34485/138‑1 (MQ=255)
                                                                  cttcCTCTCGCCTTTTTTCATCAGCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACACCCTATCC‑ACAAAACTTTTTTAGATGTAGTTAGCACAGACTGAACGAATATATTTTATTTTTTGAGAGGAGTGcc                                                                     <  1:363304/138‑1 (MQ=255)
                                                                     ccTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCaaa                                                                  <  7:277317/138‑1 (MQ=255)
                                                                        ctctcATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACg                                                              >  5:206997/1‑139 (MQ=255)
                                                                         tctcATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACg                                                              >  2:79300/1‑138 (MQ=255)
                                                                           tcATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGt                                                             <  7:61779/137‑1 (MQ=255)
                                                                           tcATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGt                                                             <  8:405846/137‑1 (MQ=255)
                                                                                 tttCATCACCTGAGCAACATTCTGATGACTTTTCTGAAAATATATACAACCTATAC‑AAAAAACTTTTTTAGATGTAGTTTGCTCAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACTTATGAACa                                                      <  3:392811/138‑1 (MQ=255)
                                                                                 tttCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACa                                                      <  7:87941/138‑1 (MQ=255)
                                                                                 tttCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACa                                                      <  1:150403/138‑1 (MQ=255)
                                                                                     atcaCCTCAGCAACATTCTGACCACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACa                                                 >  3:217338/1‑139 (MQ=255)
                                                                                     atcaCCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTATTTGAGAGGAGTGCCAAAAACGTATGAACATGACa                                                 >  3:11586/1‑139 (MQ=255)
                                                                                       caCCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACAtt                                               >  1:16389/1‑139 (MQ=255)
                                                                                       caCCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACAtt                                               <  5:386428/139‑1 (MQ=255)
                                                                                        aCCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACAtt                                               <  3:192124/138‑1 (MQ=255)
                                                                                         ccTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACAtt                                               <  1:346183/137‑1 (MQ=255)
                                                                                         ccTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACAtt                                               >  2:346183/1‑137 (MQ=255)
                                                                                         ccTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGc                                             >  3:341715/1‑139 (MQ=255)
                                                                                          cTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCg                                            <  6:69332/139‑1 (MQ=255)
                                                                                           tCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGt                                           >  8:368989/1‑139 (MQ=255)
                                                                                              gCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCtt                                        >  1:230995/1‑139 (MQ=255)
                                                                                               cAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTg                                       <  8:130822/139‑1 (MQ=255)
                                                                                                 aCATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCt                                     <  7:204510/139‑1 (MQ=255)
                                                                                                  cATTCTGAACACTTTTCTGAAAATAAATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTg                                    <  4:16877/139‑1 (MQ=255)
                                                                                                    ttCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGaa                                  >  4:58116/1‑139 (MQ=255)
                                                                                                    ttCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGaa                                  >  4:220070/1‑139 (MQ=255)
                                                                                                    ttCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGaa                                  >  5:403702/1‑139 (MQ=255)
                                                                                                    ttCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGaa                                  >  7:222830/1‑139 (MQ=255)
                                                                                                       tGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACa                                >  8:386912/1‑138 (MQ=255)
                                                                                                       tGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACa                                >  2:283866/1‑138 (MQ=255)
                                                                                                         aaCACTTTTCTGAAAATATATACAACCTATCC‑AAAAATCTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATa                              <  1:79300/138‑1 (MQ=255)
                                                                                                         aaCACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCt                                     >  6:157609/1‑131 (MQ=255)
                                                                                                         aaCACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCt                                     <  5:157609/131‑1 (MQ=255)
                                                                                                         aaCACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATa                              <  8:222830/138‑1 (MQ=255)
                                                                                                              ttttCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACt                        >  4:288251/1‑139 (MQ=255)
                                                                                                                ttCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACTTg                      >  6:117194/1‑139 (MQ=255)
                                                                                                                     aaaaTATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACTTGGa                    <  3:220070/136‑1 (MQ=255)
                                                                                                                     aaaaTATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACTTGGAGGc                 >  3:130903/1‑139 (MQ=255)
                                                                                                                     aaaaTATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGGTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACTTGGAg                   <  7:81871/137‑1 (MQ=255)
                                                                                                                               cAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACTTGGAGGCTGTTGTGGc        <  6:206997/138‑1 (MQ=255)
                                                                                                                                 aCCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAACACGTATGAACATGACATTGCGTCTTTCTGAACATAGAGCCTTGGAGGCTGTTGTGGCGAt     >  5:29027/1‑139 (MQ=255)
                                                                                                                                  ccTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACa                                                      <  3:11321/89‑1 (MQ=255)
                                                                                                                                  ccTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACa                                                      >  4:11321/1‑89 (MQ=255)
                                                                                                                                  ccTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCt                                     >  2:305934/1‑106 (MQ=255)
                                                                                                                                  ccTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCt                                     <  1:305934/106‑1 (MQ=255)
                                                                                                                                  ccTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATa                              <  6:298809/113‑1 (MQ=255)
                                                                                                                                  ccTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATa                              <  4:51719/113‑1 (MQ=255)
                                                                                                                                  ccTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATa                              >  3:51719/1‑113 (MQ=255)
                                                                                                                                  ccTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATa                              >  5:298809/1‑113 (MQ=255)
                                                                                                                                  ccTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACTTGGAGGCTGTTGTGGCGAtt    >  2:90550/1‑139 (MQ=255)
                                                                                                                                  ccTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACTTGGAGGCTGTTGTGGCGAtt    <  2:16389/139‑1 (MQ=255)
                                                                                                                                  ccTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACTTGGAGGCTGTTGTGGCGAtt    >  3:274646/1‑139 (MQ=255)
                                                                                                                                  ccTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACTTGGAGGCTGTTGTGGCGAtt    >  1:232299/1‑139 (MQ=255)
                                                                                                                                  ccTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATATTTTTGAGAGGAGTGCCAAAAACGTATGAACAAGACATTGCGTCTTGCAGAACATAGAGACTTGGAGGCTGTTGTGGCGAtt    >  5:175841/1‑139 (MQ=255)
                                                                                                                                    tATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACTTGGAGGCTGTTGTGGCGATTta  >  7:249608/1‑139 (MQ=255)
                                                                                                                                         |                                                                                                                                      
TTGCAGGGAACACCCAGCGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCCAAAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACTTGGAGGCTGTTGTGGCGATTTA  >  CP006881/3578329‑3578600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑