Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	3,725,205:1	+T	100%	intergenic (‑37/‑492)	U712_19205 ← / → U712_19210	Uncharacterized protein ywcI/Minor extracellular protease vpr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,725,205	1	.	T	83.3%	168.0 / 32.0	66	intergenic (‑37/‑492)	U712_19205/U712_19210	Uncharacterized protein ywcI/Minor extracellular protease vpr
Reads supporting (aligned to +/- strand):  ref base . (2/9);  new base T (40/12);  total (45/21)
Fisher's exact test for biased strand distribution p-value = 4.36e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

TGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAG‑GCAG‑C‑C‑T‑TTT‑A‑GCAGCCCGTTTT‑CTCAGCCGCAGCCCACGGGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAAT  >  CP006881/3725068‑3725335                                                                                                                                              |                                                                                                                                       tGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCGTCGTTTTTGCAG‑GGAG‑c‑cg                                                                                                                                        >  1:193373/1‑138 (MQ=255)  gAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAATAACATCCCTCCGCGTCTTTTTGGCAG‑GCAG‑C‑C‑t‑t                                                                                                                                     >  2:349905/1‑139 (MQ=255)  gAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAG‑GCAG‑C‑C‑t‑t                                                                                                                                     >  3:400564/1‑139 (MQ=255)  gAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAG‑GCAG‑C‑C‑t‑t                                                                                                                                     >  7:199403/1‑139 (MQ=255)    gCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGATAAACATCCCTCCGCTTCTTTTTGGCAG‑GCAG‑C‑C‑t‑ttt                                                                                                                                   >  3:140908/1‑139 (MQ=255)    gCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCGTCTTTTTGTCAG‑GCAG‑C‑C‑t‑ttg                                                                                                                                   >  3:146771/1‑138 (MQ=255)          tAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGATCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAG‑GCAG‑C‑CTT‑TTT‑a‑gcag                                                                                                                            <  1:4283/139‑1 (MQ=255)             accgCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTTGCAG‑GCAG‑C‑CTT‑TTT‑A‑GCAGccc                                                                                                                         >  1:96139/4‑139 (MQ=255)              ccgCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAG‑GCCG‑C‑CGT‑TTT‑T‑GCAGCCCg                                                                                                                        >  1:28893/3‑139 (MQ=255)               cgCGGCAGCCGCGGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACCCCCGTAAAGATACAAGCAAACGTTTGATAGAAAAACATCCCTCCGCGTCTTTTTGGCAG‑GCCG‑C‑CTT‑TTT‑A‑GCAGCCCGt                                                                                                                       >  5:72402/2‑139 (MQ=255)               cgCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCGTCTTTTTGGCAG‑GCAG‑C‑CTT‑TTT‑A‑GCAGCCCGt                                                                                                                       >  1:127420/2‑139 (MQ=255)               cgCGGCAGCCGCAGTTTTTATTTCCGGCGTCACCCAGTTCATCAAAAATACCATCCACACCCCTAAAGATAAAAGCAAACGTTTCATAGAAAAACATCCCTCCGCGTCTTTTTGGCAG‑GCAG‑CAT‑T‑TTT‑A‑GCAGcccct                                                                                                                       >  5:124056/2‑137 (MQ=255)                  ggCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAG‑GCAG‑C‑CTT‑TTT‑A‑GCAGCCCGtttt                                                                                                                    >  6:170300/1‑139 (MQ=255)                  ggCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAG‑GCAG‑C‑CTT‑TTT‑A‑GCAGCCCGtttt                                                                                                                    >  3:200187/1‑139 (MQ=255)                  ggCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAG‑GCAG‑C‑CTT‑TTT‑A‑GCAGCCCGttt                                                                                                                     >  8:66908/1‑138 (MQ=255)                  ggCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCATTTTGGCAG‑TCAG‑C‑C‑TGTTT‑A‑GCAGCCCGttt                                                                                                                     >  4:148249/1‑138 (MQ=255)                  ggCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTATCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAG‑GCCG‑C‑CTT‑TTT‑A‑GCAGCCCGttt                                                                                                                     >  8:291731/1‑138 (MQ=255)                                     tCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAG‑GCAG‑C‑CTT‑TTT‑A‑GCAGCCCGTTTT‑CTCAGCCGCAGcccgc                                                                                                   >  3:158151/1‑134 (MQ=255)                                     tCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAG‑GCAG‑C‑CTT‑TTT‑A‑GCAGCCCGTTTT‑CTCAGCCGCAGcccgc                                                                                                   >  7:262785/1‑134 (MQ=255)                                                 cAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTTGCAG‑GCCG‑C‑CTT‑TTT‑A‑GCAGCCCGTTTT‑ATAAGCCGCCGCCCGCAAGAAAAGACGGCCg                                                                                    >  5:61889/1‑139 (MQ=255)                                                    ttCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAG‑GCAG‑C‑CTT‑TTT‑A‑GCAGCCCGTTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAtt                                                                                 >  8:257671/1‑139 (MQ=255)                                                        tcaAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAG‑GCAG‑C‑CGT‑TTT‑A‑TCAGCCCGGTTT‑CTCATCCGCAGCCCGCAAGAAAAGACGGCAGAgttctt                                                                             >  3:216001/1‑139 (MQ=255)                                                         caAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAG‑GCAG‑C‑CTT‑TTT‑A‑GCAGCCCGTTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAtttagtt                                                                            >  5:75339/1‑135 (MQ=255)                                                            aaaGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAG‑GCAG‑C‑CTT‑TTT‑A‑GCAGCCCGTTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAtttcttttct                                                                         >  7:251906/1‑139 (MQ=255)                                                                aCCATCAACACCCGAAACGATACAAGAAAACTTTTCATAGAAAAACATCCCTCCGCTTGTTTTTGGCAG‑GCAG‑C‑CTT‑TTT‑C‑GCAGCCGGTTTT‑CACAGCCGCCCCCCGCAACAAAAGACGGCCGATTTCTTTTCTCGCa                                                                     <  7:257671/139‑1 (MQ=255)                                                                   aTCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAC‑GCAG‑C‑CTT‑TTT‑A‑GCAGCCCGTTTT‑CTCAGCCGCACCCCCCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAAc                                                                  >  4:143474/1‑139 (MQ=255)                                                                      caccCCCGTAACGATACACGCAAACGTTTCATACAAAAACAGCCCGCCGCTTCTTTTGGGCCG‑GCCG‑C‑CTT‑TTT‑A‑GCCGCCCGTTTT‑CGCAGCCGCCGCCCGCAAGAAACGACGGCCGATTTCTTTTCTCGCAAACTAg                                                               <  5:170300/139‑1 (MQ=255)                                                                        cacCCGTAAAGATACAAGCAAACGTGTCATAGAAAAACCTCCCTCCGCTTCGTTTTGGCAG‑GCAG‑C‑CTT‑TTT‑A‑GCAGCCCGTTTT‑CTCAGCCGCAGCCCGCAAGCAACGACGGCCGATGTATTTTCGCGCAAACTAGCg                                                             >  4:353614/1‑139 (MQ=255)                                                                             gTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAG‑GCAG‑C‑CTT‑TTT‑A‑GCAGCCCGTTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGGAAACTAGCGGctct                                                        >  6:391037/1‑139 (MQ=255)                                                                             gTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAG‑GCAG‑C‑CTT‑TTT‑A‑GCAGCCCGTTTT‑CTAAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGctc                                                         >  8:47837/1‑138 (MQ=255)                                                                              tAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAG‑GCAG‑C‑CTT‑TTT‑A‑GCAGCCCGTTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGctc                                                         >  1:215999/1‑137 (MQ=255)                                                                              tAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAG‑GCAG‑C‑CTT‑TTT‑A‑GCAGCCCGTTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGctc                                                         >  8:141297/1‑137 (MQ=255)                                                                              tAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAG‑GCAG‑C‑CTT‑TTT‑A‑GCAGCCCGTTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGctc                                                         >  1:54496/1‑137 (MQ=255)                                                                              tAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCCCCGCGTCTTTTTGGCAG‑GCAG‑C‑CTT‑TTT‑A‑GCAGCCCGGTTT‑CTCAGCCGCCGCCCGCAAGAAAAGACGGCCGAGTTCATTTCTCGCAAACAAGCGGctc                                                         >  1:79504/1‑137 (MQ=255)                                                                                aactaaCAAGAAATCGTTTCATAGAAAAACTTCCCTCGGTGTGTTTTTGCCCG‑GCCG‑C‑T‑T‑TTT‑ATGCAGCCGGTTTG‑CTCAGCCCCCGCCCGAAAAAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAg                                                      <  2:107167/133‑1 (MQ=255)                                                                                 aGATACAACCAAACTTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCCG‑GCAG‑CTT‑T‑TTT‑A‑GCCGCCGGTTTT‑CTCCCCCGCCGCCGGAAACAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCa                                                    <  7:88928/139‑1 (MQ=255)                                                                                     aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAG‑GCAG‑C‑CTT‑TTT‑A‑GCAGCCCGtt                                                                                                                      <  1:28423/70‑1 (MQ=255)                                                                                     aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAG‑GCAG‑C‑CTT‑TTT‑A‑GCAGCCCGtt                                                                                                                      >  2:28423/1‑70 (MQ=255)                                                                                     aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAG‑GCAG‑C‑CTT‑TTT‑A‑GCAGCCCGTTTT‑CTCAGCCGCCGCCCGCAAGAAAAGACGGCCGATTTCGTTTCTCGCAAACTAGCGGCTCTAGCAAAAc                                                >  7:10708/1‑139 (MQ=255)                                                                                     aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAG‑GCAG‑C‑CTT‑TTT‑A‑GCAGCCCGTTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAAc                                                >  3:141809/1‑139 (MQ=255)                                                                                     aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAG‑GCAG‑C‑CTT‑TTT‑A‑GCAGCCCGTTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCAAGCaaaa                                                 >  8:88928/1‑138 (MQ=255)                                                                                     aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAG‑GCAG‑C‑CTT‑TTT‑A‑GCAGCCCGTTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAAATAGCGGCTCAAGCAAAAc                                                >  3:126785/1‑139 (MQ=255)                                                                                     aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAG‑GCAG‑C‑CTT‑TTT‑A‑GCAGCCCGTTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAGTTCTTTTCTCGCAAGCTAGCGGCGCGAGCACAAc                                                >  6:150847/1‑139 (MQ=255)                                                                                       aaGCAAACGTTTCATAGAAAAACCTCCCAACGCGTCGTTTTGGCAG‑GCCC‑C‑CAT‑TTT‑T‑TCAGCCCCGTTT‑ATACGCCGCCGCCCCCCAGAAAAACCCGCCCAATTATTTTAACCCAAACGAGCCGCACTAGCcaaaaa                                               >  2:18345/1‑136 (MQ=255)                                                                                       aaGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAG‑GCAG‑C‑CTT‑TTT‑A‑GCAGCCCGTTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACt                                               >  2:256650/1‑138 (MQ=255)                                                                                       aaGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAG‑GCAG‑C‑CTT‑TTT‑A‑GCAGCCCGTTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTAGCAAACTAGCGGCTCTAGCAAAACt                                               >  3:147980/1‑138 (MQ=255)                                                                                        cccAAACGTTTAATATAAAAACATCACTCCGGTTTTTTTTGGCCGCCCCT‑T‑T‑T‑TTT‑A‑GCCCCCCTTTTT‑CACCCCCCCCCCCCCAAACAAACGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCa                                             <  4:275910/137‑1 (MQ=255)                                                                                         gCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAG‑GCAG‑C‑CTT‑TTT‑A‑GCCGCCCGTTTT‑CGCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt                                            <  7:224776/139‑1 (MQ=255)                                                                                         ccAACCGTTTCATACAAAAACACCCCTCCGCTTCTTTTGGCCCG‑GCAG‑C‑CTT‑TTT‑A‑CCACCCCGTTTT‑CCCCGCCCCCGCCCCCAACAAAACACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt                                            <  8:199403/138‑1 (MQ=255)                                                                                         ccAAACTTTTCATCTAAAAAAACCCCTCCGATTCTTTTTGCCAG‑GCCG‑C‑CTT‑TTT‑C‑CCCGCCCGTTTT‑CTCAGCCGCAGCCCCAAACAAACCACGGCCGATTTATTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt                                            <  3:360237/138‑1 (MQ=255)                                                                                         ccAAACTTTTAATATAAAAACAACCCGCCGCTTATTTTTGCCGG‑GCCG‑C‑G‑T‑TTT‑ACCCAGCCGGTTTT‑CTCCGCCCCCCCCCCCAATAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt                                            <  4:141809/138‑1 (MQ=255)                                                                                         ccAAACGTTTCATACAAAAACAGCCCGCCGCTTCTTTTTGGCCG‑GCAG‑C‑CTT‑TTT‑A‑GCAGCCCGTTTG‑CTCACCCGCCCCCCCCAACAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt                                            <  7:256564/138‑1 (MQ=255)                                                                                         ccAAACGTTTAATACAAAAACACCCCTCCGCTTTTTTTTGGCCG‑CCAG‑CGT‑T‑TTT‑A‑GCCCCCCGTTTT‑CTCCCCCGCCCCCCCAAACAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt                                            <  5:370053/138‑1 (MQ=255)                                                                                         ccAAACGTTCCAAAGAAAAACATCCCTCCGCTTCTTTTTGGCTG‑GATG‑CTT‑T‑TTT‑A‑GCCGCCGGTTTT‑CTCAGCCGCCCCCCGAAACAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt                                            <  7:291731/138‑1 (MQ=255)                                                                                           aaccGTTTCATATAAAAACATCCCTCCGCTTATTTTTGGCAG‑CCCGAT‑T‑T‑TTT‑A‑GCACCCGTTTTT‑CTCACCCGCAGCCCTAAACAAACCACGGCCGATTTCTTTTGTCGCAAACTAGCGGCTCTAGCAAAACTCATGa                                          <  4:147980/136‑1 (MQ=255)                                                                                            aaCGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAG‑GCAG‑C‑CTT‑TTT‑A‑GCAGCCCGTTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTAGCAAACTAGCGGCTCTAGAAAAACTCATTAc                                         >  1:134789/1‑139 (MQ=255)                                                                                             aggTTTAATAAAAAAACATCCCTCGTTTTTTTTTTGGCCG‑GCAG‑CTT‑T‑TTT‑C‑GCA‑CCCGTTTTACTCCCCCCCCGCCCCAAAAAAACCACGGCCGATTTCTTTTCTCGAAAACTAGCGGCTCTAGCAAAACTCATGAct                                        <  4:158151/137‑1 (MQ=255)                                                                                             aCGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAG‑GCAG‑C‑CTT‑TTT‑A‑GCAGCCCGTTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTATTTTCTCGCAAACTAGCGGCTCGAGCAAAAAGCATGAcg                                        >  3:11818/1‑138 (MQ=255)                                                                                             aCGTTTCATAGAAAAACATCCCTCCGCTGCTTTTTGGCAG‑GCAG‑C‑CTT‑TTT‑A‑GCAGCCCGTTTT‑CGCCGCCCCAGCCCGCAACAAACGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct                                        <  7:66908/139‑1 (MQ=255)                                                                                             aCGTTTCATACAAAAACATCCCTCCGCTTATTTTTGGCAG‑GCAG‑C‑CTT‑TTT‑A‑GCAGCCCGTTTT‑CACACCCGCAGCCCGCAATAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct                                        <  1:256650/139‑1 (MQ=255)                                                                                             aCGTTTCATACAAAAACACCCCGCCGCTGCTTTTTGGCCG‑CCAG‑C‑CTT‑TTT‑C‑GCAGCCCGTTTT‑CTACCCCGCACCCCGCAATAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct                                        <  7:47837/139‑1 (MQ=255)                                                                                             aCGTTTAATACAAAAACACCCCTCCGTTTGTTTTTGCCCG‑CACG‑C‑CTT‑TTT‑C‑GCAGCCGTTTTT‑CTCTGCCGCCGCCCGAAATAAACGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct                                        <  8:251906/139‑1 (MQ=255)                                                                                                  tCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAG‑GCAG‑C‑CTT‑TTT‑A‑GCAGCCCGTTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCGAGCAAAACTCATGCCTCGAt                                    >  5:22612/1‑138 (MQ=255)                                                                                                   cATAGAAAAACATCCCTCCGCTTCTTTTTGGCAG‑GCAG‑C‑CTT‑TTT‑A‑GCAGCCCGTTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGCTTTATTTTATCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGa                                  >  5:329831/1‑139 (MQ=255)                                                                                                    caccaaaaaCATCCCTCCGCTTCTTTTTGGCCG‑CCAG‑C‑C‑T‑TTTAA‑GCCCCCGTTTTT‑CACACCCCCACCCCGCAACAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGAt                                 <  6:305581/135‑1 (MQ=255)                                                                                                        aaaaaCATCCCTCCGCTTCTTTTTGGCAG‑GCAG‑C‑CTT‑TTT‑A‑GCAGCCCGTTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGAc                             >  2:290193/1‑139 (MQ=255)                                                                                                        aaaaaCATCCCTCCGCTTCTTTTTGGCAG‑GCAG‑C‑CTT‑TTT‑A‑GCAGCCCGTTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGAc                             >  5:333614/1‑139 (MQ=255)                                                                                                        aaaaaCATCCCTCCGCTTCTTTTTGGCAG‑GCAG‑C‑CTT‑TTT‑A‑GCAGCCCGTTTT‑CTCAGCCGCAGCCCGCAAGAAAACACGGCCGCTTTCTTTTATCGCAAACAAGCGGCTCTAGCAAAACTCATGACGCGATGATAGAc                             >  7:315088/1‑139 (MQ=255)                                                                                                          aaaCATCCCTCCGCTTCTTTTTGGCAG‑GCAG‑C‑CTT‑TTT‑A‑GCAGCCCGTTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAACCTCATGACTGTATGATAGACCg                           >  8:8881/1‑139 (MQ=255)                                                                                                                  cTCCGCTTCTTTTTGGCAG‑GCAG‑C‑CTT‑TTT‑A‑GCAGCCCGTTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGaaa                     >  7:241909/1‑137 (MQ=255)                                                                                                                  cTCCGCGTCTTTTTGGCAG‑GCAG‑C‑CTT‑TTT‑A‑GCAGCCCGTTTT‑CTAAGCCGCAGCCCCCAAGAAAAGACGCCCGATTTATTTTATCGCAAAATAGCGGCTATAGGAAAACTAATTACTCTAAGATATACCGTGGAAag                    >  6:134157/1‑138 (MQ=255)                                                                                                                                    g‑gcag‑C‑CTT‑TTT‑A‑GCAGCCCGTTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTAGCAAACTAGCGGCTCTAGCAAAACTCATTACTATATGATTGCCACTGGAAAGAGAATTCTTTGTCACAat  >  4:290715/1‑138 (MQ=255)                                                                                                                                              |                                                                                                                                       TGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAG‑GCAG‑C‑C‑T‑TTT‑A‑GCAGCCCGTTTT‑CTCAGCCGCAGCCCACGGGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAAT  >  CP006881/3725068‑3725335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑