Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 770,560 G→A 7.6% V146I (GTT→ATT)  U712_03720 → Putative aldehyde dehydrogenase yfmT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP006881770,5600GA 7.6% 160.5 / 2.9 53V146I (GTT→ATT) U712_03720Putative aldehyde dehydrogenase yfmT
Reads supporting (aligned to +/- strand):  ref base G (26/22);  new base A (2/2);  total (29/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.40e-01

AAAGCAGCTTTTGAAATCGGTCTTGTCAAAAACATCATAAAAGAAGCGGCAACGTTCCCAATCCGCATGGAGGGGAAAATTCTTCCGTCAACAATAGATGGCAAGGAAAACAGATTATATCGCGTGCCGGCAGGCGTTGTCGGTGTCATCAGTCCGTTTAACTTCCCATTCTTTTTATCTATGAAATCAGTGGCGCCTGCACTCGGAGCCGGCAATGGCGTTGTGCTAAAGCCGCATGAGGAAACGCCGATTTGCGGCGGAACGCTGATTGCGA  >  CP006881/770425‑770698
                                                                                                                                       |                                                                                                                                          
aaaGCAGCTTTTGAAATCGGTCTTGTCAAAAACATCATAAAAGAAGCGGCAACGTTCCCAATCCGCATGGAGGGGAAAATTCTTCCGTCAACAATAGATGGCAAGGAAAACAGATTATATCGCGTGCCGGCAGGCGTTg                                                                                                                                         >  6:218516/1‑139 (MQ=255)
aaaGCAGCTTTTGAAATCGGTCTTGTCAAAAACATCATAAAAGAAGCGGCAACGTTCCCAATCCGCATGGAGGGGAAAATTCTTCCGTCAACAATAGATGGCAAGGAAAACAGATTATATCGCGTGCCGGCAGGCGTTg                                                                                                                                         >  3:261107/1‑139 (MQ=255)
aaaGCAGCTTTTGAAATCGGTCTTGTCAAAAACATCATAAAAGAAGCGGCAACGTTCCCAATCCGCATGGAGGGGAAAATTCTTCCGTCAACAATAGATGGCAAGGAAAACAGATTATATCGCGTGCCGGCAGGCGTTg                                                                                                                                         >  2:107404/1‑139 (MQ=255)
aaaGCAGCTTTTGAAATCGGTCTTGTCAAAAACATCATAAAAGAAGCGGCAACGTTCCCAATCCGCATGGAGGGGAAAATTCTTCCGTCAACAATAGATGGCAAGGAAAACAGATTATATCGCGTGCCGGCAGGCGTTg                                                                                                                                         >  8:338440/1‑139 (MQ=255)
aaaGCAGCTTTTGAAATCGGTCATGTCAAAAACATCATAAAAGAAGCGGCAACGTTCCCAATCCGCATGGAGGGGAAAATTCTTCCGTCAACAATAGATGGCAAGGAAAACAGATTATATCGCGTGCCGGCAGGCGTTg                                                                                                                                         >  3:337704/1‑139 (MQ=255)
  agcagcTTTTGAAATCGGTCTTGTCAAAAACATCATAAAAGAAGCGGCAACGTTCCCAATCCGCATGGAGGGGAAAATTCTTCCGTCAACAATAGATGGCAAGGAAAACAGATTATATCGCGTGCCGGCAGGCGTTGTc                                                                                                                                       <  7:195468/139‑1 (MQ=255)
    cagcTTTTGAAATCGGTCTTGTCAAAAACATCATAAAAGAAGCGGCAACGTTCCCAATCCGCATGGAGGGGAAAATTCTTCCGTCAACAATAGATGGCAAGGAAAACAGATTATATCGCGTGCCGGCAGGCGTTGTCgg                                                                                                                                     <  8:100944/139‑1 (MQ=255)
     agcTTTTGAAATCGGACTTGTCAAAAACATCATAAAAGAAGCGGCAACGTTCCCAATCCGCATGGAGGGGAAAATTCTTCCGTCAACAATAGATGGCAAGGAAAACAGATTATATCGCGTGCCGGCAGGCGTTGTCGgt                                                                                                                                    <  7:269294/139‑1 (MQ=255)
       cTTTTGAAATCGGTCTTGTCAAAAACATCATAAAAGAAGCGGCAACGTTCCCAATCCGCATGGAGGGGAAAATTCTTCCGTCAACAATAGATGGCAAGGAAAACAGATTATATCGCGTGCCGGCAGGCGTTGTCGgtgt                                                                                                                                  >  5:106889/1‑139 (MQ=255)
           tGAAATCGGTCTTGTCAAAAACATCATAAAAGAAGCGGCAACGTTCCCAATCCGCATGGAGGGGAAAATTCTTCCGTCAACAATAGATGGCAAGGAAAACAGATTATATCGCGTGCCGGCAGGCGTTGTCGGTGtcat                                                                                                                               <  4:135632/138‑1 (MQ=255)
             aaaTCGGTCTTGTCAAAAACATCATAAAAGAAGCGGCAACGTTCCCAATCCGCATGGAGGGGAAAATTCTTCCGTCAACAATAGATGGCAAGGAAAACAGATTATATCGCGTGCCGGCAGGCGTTGTCGGTGtcatca                                                                                                                             <  5:20446/138‑1 (MQ=255)
              aaTCGGTCTTGTCAAAAACATCATAAAAGAAGCGGCAACGTTCCCAATCCGCATGGAGGGGAAAATTCTTCCGTCAACAATAGATGGCAAGGAAAACAGATTATATCGCGTGCCGACAGGCGTTGTCGGTGTCATCAGt                                                                                                                           <  6:332118/139‑1 (MQ=255)
              aaTCGGTCTTCTCAAAAACATCATAACAGACGCGGCAACGTTCCCAATCCGCATGGAGGGGAAAATTCTTCCGTCAACAATAGATGGCAAGGAAAACAGATTATATCGCGTGCCGGCAGGCGTTGTCGGTGTCATCAGt                                                                                                                           <  2:389907/139‑1 (MQ=255)
                 cGGTCTTGTCAAAAACATCATAAAAGAAGCGGCAACGTTCCCAATCCGCATGGAGGGGAAAATTCTTCCGTCAACAATAGATGGCAAGGAAAACAGATTATATCGCGTGCCGGCAGGCGTTGTCGGTGTCATCAGTCCg                                                                                                                        >  7:252455/1‑139 (MQ=255)
                 cGGTCTTGTCAAAAACATCATAAAAGAAGCGGCAACGTTCCCAATCAGCATGGAGGGGAAAATTCTTCCGTCAACAATAGATGGCAAGGAAAACAGATTATATCGCGTGCCGGCAGGCGTTGTCGGTGTCATCAGTCCg                                                                                                                        >  1:11956/1‑139 (MQ=255)
                             taatatcatAAAAGAAGCGGCAAAGTTCCGAATCCGCATGGAGGGGAAAATTCTTCCGTCAACAATAGATGGCTAGGAAAACAGATTATATCGCGTGCCGGCAGGCGTTGTCGGTGTCATCAGTCCGTTTAACTTCCCa                                                                                                            <  4:337704/135‑1 (MQ=255)
                             aaaCATCATAAAAGAAGCGGCAACGTTCCCAATCCGCATGGAGGGGAAAATTCTTCCGTCAACAATAGATGGCAAGGAAAACAGATTATATCGCGTGCCGGCAGGCGTTGTCGGTGTCATCAGTCCGTTTAACTTCCCa                                                                                                            <  5:114771/139‑1 (MQ=255)
                                     tAAAAGAAGCGGCAACGTTCCCAATCCGCATGGAGGGGAAAATTCTTCCGTCAACAATAGATGGCAAGGAAAACAGATTATATCGCGTGCCGGCAGGCGTTGTCGGTGTCATCAGTCCGTTTAACTTCCCATTCttttt                                                                                                    <  4:270634/139‑1 (MQ=255)
                                           aagCGGCAACGTTCCCAATCCGCATGGAGGGGAAAATTCTTCCGTCAACAATAGATGGCAAGGAAAACAGATTATATCGCGTGCCGGCAGGCGTTGTCGGTGTCATCAGTCCGTTTAACTTCCCATTCTTTTTATCTAt                                                                                              <  8:396947/139‑1 (MQ=255)
                                              cGGCAACGTTCCCAATCCGCATGGAGGGGAAAATTCTTCCGTCAACAATAGATGGCAAGGAAAACAGATTATATCGCGTGCCGGCAGGCGTTGTCGGTGTCATCAGTCCGTTTAACTTCCCATTCTTTTTATCTATGaa                                                                                           <  5:241888/139‑1 (MQ=255)
                                                    cGTTCCCAATCCGCTTGGAGTGGAAAATTCTGCCGTCAACAATAGATGGCAAGGAAAACAGATTATATCGCGGGCCGGCAGGCGTTGTCGGTGTCATCAGTCCGTTTAACTTCCCATTCTTTTTATCTATGAAATCAGt                                                                                     <  4:261107/139‑1 (MQ=255)
                                                                 cATGGAGGGGAAAATTCTTCCGTCAACAATAGATGGCAAGGAAAACAGATTATATCGCGTGCCGGCAGGCGTTGTCGGTGTCATCAGTCCGTTTAACTTCCCATTCTTTTTATCTATGAAATCAGTGGCGCCTGCACTc                                                                        >  3:246968/1‑139 (MQ=255)
                                                                 cATGGAGGGGAAAATTCTTCCGTCAACAATAGATGGCAAGGAAAACAGATTATATCGCGTGCCGGCAGGCGTTGTCGGTGTCATCAGTCCGTTTAACTTCCCATTCTTTTTATCTATGAAATCAGTGGCGCCTGCACTc                                                                        >  6:377045/1‑139 (MQ=255)
                                                                 cATGGAGGGGAAAATTCTTCCGTCAACAATAGATGGCAAGGAAAACAGATTATATCGCGTGCCGGCAGGCGTTGTCGGTGTCATCAGTCCGTTTAACTTCCCATTCTTTTAATCTATGAAATCAGTTGCGCCTGCACTc                                                                        >  1:151326/1‑139 (MQ=255)
                                                                  aTGGAGGGGAAAATTCTTCCGTCAACAATAGATGGCAAGGAAAACAGATTATATCGCGTGCCGGCAGGCGTTGTCGGTGTCATCAGTCCGTTTAACTTCCCATTCTTTTTATCTATGAAATCAGTGGCGCCTGCACTCg                                                                       <  7:190319/139‑1 (MQ=255)
                                                                            aaaTTCTTCCGTCAACAATAGATGGCAAGGAAAACAGATTATATCGCGTGCCGGCAGGCGTTGTCGGTGTCATCAGTCCGTTTAACt                                                                                                                 >  8:175598/1‑87 (MQ=255)
                                                                            aaaTTCTTCCGTCAACAATAGATGGCAAGGAAAACAGATTATATCGCGTGCCGGCAGGCGTTGTCGGTGTCATCAGTCCGTTTAACt                                                                                                                 <  7:175598/87‑1 (MQ=255)
                                                                                    ccGTCAACAATAGATGTCAAGGAAAACAGATTATATCGCGTGCCGGCAGGCGTTGTCGGTGTCATCAGTCCGTTTAACTTCCCATTCTTTTTATCTATGAAATCAGTGGCGCCTGCACTCGGAGCCGGCAATGGCGt                                                       <  5:377045/137‑1 (MQ=255)
                                                                                    ccGTCAACAATAGATGGCAAGGAAAACAGATTATATCGCGTGCCGGCAGGCGTTGTCGGTGTCATCAGTCCGTTTAACTTCCCATTCTTTTTATCTATGAAATCAGTGGCGCCTGCACTCGGAGCCGGCAATGGCGt                                                       <  7:112300/137‑1 (MQ=255)
                                                                                            aaTAGATGGCAAGGAAAACAGATTATATCGCGTGCCGGCAGGCGTTGTCGGTGTCATCAGTCCGTTTAACTTCCCATTCTTTTTATCTATGAAATCAGTGGCGCCTGCACTCGGAGCCGGCAATGGCGTTGTGCTAAAg                                             <  7:143639/139‑1 (MQ=255)
                                                                                                 aTGGCAAGGAAAACAGATTATATCGCGTGCCGGCAGGCGTTGTCGGTGTCATCAGTCCGTTTAACTTCCCCTTCTTTTTATCTATGAATTCCGTGGCGCCGGCCCGCGGAGCCGGCAATGGCGTTGTGCTTAAGCCg                                          >  2:376427/1‑137 (MQ=255)
                                                                                                 aTGGCAAGGAAAACAGATTATATCGCGTGCCGGCAGGCGTTGTCGGTGTCATCAGTCCGTTTAACTTCCCATTCTTTTTATCTATGAAATCAGTGGCGCCTGCACTCGGAGCCGGCAATGGCGTTGTGCTAAAGCCGCa                                        <  7:338440/139‑1 (MQ=255)
                                                                                                   ggCAAGGAAAACAGATTATATCGCGTGCCGGCAGGCGTTGTCGGTGTCCTCAGTCCGGTTACCTTCCCATTCTTTTTATCTATGAAATCAGTGGCGCCTGCACTCGGCGCCGGCATTGGCGTTGTGCTAAAGCCGCagg                                      >  2:50284/1‑137 (MQ=255)
                                                                                                   ggCAAGGAAAACAGATTATATCGCGTGCCGGCAGGCGTTGTCGGTGTCATCAGTCCGTTTAACTTCCCATTCTTTTTATCTATGAAATCAGTGGCGCCTGCACTCGGAGCCGGCAATGGCGTTGTGCTAAAGCCGCAt                                       >  2:13208/1‑138 (MQ=255)
                                                                                                   ggCAAGGAAAACAGATTATATCGCGTGCCGCCAGGCGTTGTCGGTGTCATCAGTCCGTTTAACTTCCCATTCTATTTATCTATGAAATCAGTCGCGCCTGCACTCGGAGCCGGCAATGGCCTTGAGCTAAAGCCGCAt                                       >  3:361207/1‑138 (MQ=255)
                                                                                                     cAAGGAAAACAGATTATATCGCGTGCCGGCAGGCGTTGTCGGTGTCATCAGTCCGTTTAACTTCCCATTCTTTTTATCAATGAAATCAGTGGCGCCTGCACTCGGAGCAGGCATTGGCGTTGTGCTAAAGCCGCATGAg                                    >  1:246161/1‑139 (MQ=255)
                                                                                                      aaGGAAAACAGATTATATCGCGTGCCGGCAGGCGTTGTCGGTGTCATCAGTCCGTTTAACTTCCCATTCTTTTTATCTATGAAATCAGTGGCGCCTGCACTCGGAGCCGGCAATGGCg                                                        >  4:222165/1‑118 (MQ=255)
                                                                                                      aaGGAAAACAGATTATATCGCGTGCCGGCAGGCGTTGTCGGTGTCATCAGTCCGTTTAACTTCCCATTCTTTTTATCTATGAAATCAGTGGCGCCTGCACTCGGAGCCGGCAATGGCg                                                        <  3:222165/118‑1 (MQ=255)
                                                                                                      aaGGAAAACAGATTATATCGCGTGCCGGCAGGCGTTGTCGGTGTCATCAGTCCGTTTAACTTCCCATTCTTTTTATCTATGAAATCAGTGGCGCCTGCACTCGGAGCCGGCAATGGCGTTGTGCTAAAGCCGCATGAg                                    >  3:177926/1‑138 (MQ=255)
                                                                                                                 aTTATATCGCGTGCCGGCAGGCGTTGTCGGTGTCATCAGTCCGTTTAACTTCCCATTCTTTTTATCTATGAAATCAGTGGCGCCTGCACTCGGAGCCGGCAATGGCGTTGTGCTAAAGCCGCATGAGGAAACGCCGAtt                        >  2:213183/1‑139 (MQ=255)
                                                                                                                      atCGCGTGCCGGCAGGCGTTGTCGGTGTCATCAGTCCGTTTAACTTCCCATTCTTTTTATCTATGAAATCAGTGGCGCCTGCACTCGGAGCCGGCAATGGCGTTGTGCTAAAGCCGCATGAGGAAACGCCGATTTgcgg                   >  5:127930/1‑139 (MQ=255)
                                                                                                                        cgcgTGCCGGCAGGCGTTGTCGGTGTCATCAGTCCGTTTAACTTCCCa                                                                                                            <  2:204363/48‑1 (MQ=255)
                                                                                                                        cgcgTGCCGGCAGGCGTTGTCGGTGTCATCAGTCCGTTTAACTTCCCa                                                                                                            >  1:204363/1‑48 (MQ=255)
                                                                                                                          cgTGCCGGCAGGCTTTGTCGGTGTCATCAGTCCGTTTAACTTCCCATTCTTTTTATCTATGAAATCAGTGGCGCCTGCACTCGGAGCCGGCAATGGCGTTGTGCTAAAGCCGCATGAGGAAACGCCGATTTGcggcgg                >  3:412993/1‑138 (MQ=255)
                                                                                                                          cgTGCCGGCAGGCGTTGTCGGTGTCATCAGTCCGTTTAACTTCCCATTCTTTTTATCTATGAAATCAGTGGCGCCTGCACTCGGa                                                                     >  7:150606/1‑85 (MQ=255)
                                                                                                                          cgTGCCGGCAGGCGTTGTCGGTGTCATCAGTCCGTTTAACTTCCCATTCTTTTTATCTATGAAATCAGTGGCGCCTGCACTCGGa                                                                     <  8:150606/85‑1 (MQ=255)
                                                                                                                          cgTGCCGGCAGGCGTTGTCGGTGTCATCAGTCCGTTTAACTTCCCATTCTTTTTATCTATGAAATCAGTGGCGCCTGCACTCGGAGCCGGCAATGGCGTTGTGCTAAAGCCGCAGGAGGAAACGCCGATTTGcggcgg                >  6:402932/1‑138 (MQ=255)
                                                                                                                           gTGCCGGCAGGCGTTGTCGGTGTCATCAGTCCGTTTAACTTCCCATTCTTTTTATCTATGAAATCAGTGGCGCCTGCACTCGGAGCCGGCAATGGCGTTGTGCTAAAGCCGCATGAGGAAACGCCGATTTGCGGCGGaa              >  1:66816/1‑139 (MQ=255)
                                                                                                                           gTGCCGGCAGGCGTTGTCGGTGTCATCAGTCCGTTTAACTTCCCATTCTTTTTATCTATGAAATCAGTGGCGCCTGCACTCGGAGCCGGCAAAGGCGTTGTGCTAAAGCAGCATGAGGAAACTCCGATTTGCGGCGGaa              >  3:256500/1‑139 (MQ=255)
                                                                                                                           gTGCCGGCAGGCATTGTCGGTGTCATCAGTCCGTTTAACTTCCCATTCTTTTTATCTATGAAATCAGTGGCGCCTGCACt                                                                         <  4:96950/80‑1 (MQ=255)
                                                                                                                           gTGCCGGCAGGCATTGTCGGTGTCATCAGTCCGTTTAACTTCCCATTCTTTTTATCTATGAAATCAGTGGCGCCTGCACt                                                                         >  3:102605/1‑80 (MQ=255)
                                                                                                                           gTGCCGGCAGGCATTGTCGGTGTCATCAGTCCGTTTAACTTCCCATTCTTTTTATCTATGAAATCAGTGGCGCCTGCACt                                                                         <  4:102605/80‑1 (MQ=255)
                                                                                                                           gTGCCGGCAGGCATTGTCGGTGTCATCAGTCCGTTTAACTTCCCATTCTTTTTATCTATGAAATCAGTGGCGCCTGCACt                                                                         >  3:96950/1‑80 (MQ=255)
                                                                                                                                       gTTGTCCGTGTCCGCTTTCCTATTAACTAGCCATTCTTTATATATATGAAATCAGAGGCGCGTGCACTCGGAGCCGGAATTGGCTTTGTGCTAAAGACGCATGAGGAAACGACGATTTGCGGCGGAACGCTGATTGCGa  <  2:11956/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                          
AAAGCAGCTTTTGAAATCGGTCTTGTCAAAAACATCATAAAAGAAGCGGCAACGTTCCCAATCCGCATGGAGGGGAAAATTCTTCCGTCAACAATAGATGGCAAGGAAAACAGATTATATCGCGTGCCGGCAGGCGTTGTCGGTGTCATCAGTCCGTTTAACTTCCCATTCTTTTTATCTATGAAATCAGTGGCGCCTGCACTCGGAGCCGGCAATGGCGTTGTGCTAAAGCCGCATGAGGAAACGCCGATTTGCGGCGGAACGCTGATTGCGA  >  CP006881/770425‑770698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑