| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 787,781 | T→A | 15.5% | L323F (TTA→TTT) | U712_03815 ← | Fe(3+)‑citrate import system permease protein yfmD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 787,781 | 0 | T | A | 15.5% | 108.2 / 5.4 | 58 | L323F (TTA→TTT) | U712_03815 | Fe(3+)‑citrate import system permease protein yfmD |
| Reads supporting (aligned to +/- strand): ref base T (20/28); new base A (5/4); total (25/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAATGCACCGATTCCAATGCTGATGACGGACAGAACGATTAAAATCACGGCCAATATGAGTATCGCCAGTAAGGGGCGT‑TTTTGTTTTCTGGTTGTTTTCTTCATTTCAGGTTTCG‑CCCTTTCCTTGCCAAATATAAGAAAAACGGCGTTCCGATAATCGCGGTGACAATGCCGACCGGTGATTCATAAGGAAATGCAATCCATCTGGCCAGAACATCTGCGTACACCAGCAAAATGGCACCGAACAGTGCCGAAAACGGAAGCACGTAT > CP006881/787647‑787916 | taATGCACCGATTCCAATGCTGATGACGGACAGAACGATTAAAATCACGGCCAATATGAGTATCGCCAGTAAGGGGCGT‑GGTTGTTTTGTGGTTGTGTTGTGGCTTTGAGGTTTGG‑GCCGTTCCTTGGCAAATATaat > 2:50018/1‑137 (MQ=255) tGCACCGATTCCAATGCTGATGACGGACAGAACGATTAAAATCACGGCCAATATGAGTATCGCCAGTAAGGGGCGT‑GTTTTTTTTGTTGTTGTTTTGTTTTTTTAAGGTTTGG‑CCCGTGGCGTGCCGAAAATaaaaaa > 1:371017/1‑138 (MQ=255) cACCGATTCCAATGCTGATGACGGACAGAACGATTAAAATCACGGCCAATATGAGTATCGCCAGTAAGGGGCGT‑GTTTGTTTTTTGTTTTTTTTTTTTAATTAAGGGTTAT‑GCCTTTGCTTGGGAAATAAAATaaaaa > 8:378359/1‑138 (MQ=255) ccGATTCCAATGCTGATGACGGACAGAACGATTAAAATCACGGCCAATATGAGTATCGCCAGTAAGGGGCGTGTTTTTTTTAGTGGTTGTTTT‑TTGATTTAGGGTTTTGCCCCTTACGTTGCCAAA‑ATAAAAAAAACgg > 1:318486/1‑139 (MQ=255) cGATTCCAATGCTGATGACGGACAGAACGATTAAAATCACGGCCAATATGAGTATCGCCAGTAAGGGGCGTGTTTTTTTTTTTGGGTGTTTGTTAAATTGTGGTTTTGG‑CCGTTGCGTTGCC‑AATATAAGAAAAAAGGc > 5:316534/1‑139 (MQ=255) cGATTCCAATGCTGATGACGGACAGAACGATTAAAATCACGGCCAATATGAGTATCGCCAGTAAGGGGCGT‑GTTTGTTTTTTTGTTGTTTTGTTTATTTTAGGTTTGG‑CGCGGTGCATTGCAAAAATAAGaaaaaacgc > 4:93239/1‑135 (MQ=255) cGATTCCAATGCTGATGACGGACAGAACGATTAAAATCACGGCCAATATGAGTATCGCCAGTAAGGGGCGT‑GTTTGTTTTGTGGTTGTTTTGTTGATTTAAGGTTTAG‑CCCGTTCGTTGACAAATATAATAAAAACGGc > 7:386662/1‑139 (MQ=255) cGATTCCAATGCTGATGACGGACAGAACGATTAAAATCACGGCCAAGATGAGTATCGCCAGTAAGGGGCGT‑TTTTTGTTTTTGGGTGGTTTATTGATTTCATGGTTGG‑CCCCTTACTTGCCCAATATTATAAAAACAGc > 7:4642/1‑139 (MQ=255) aTTCCAATGCTGATGACGGACAGAACGATTAATATCACGGCCAATATGAGTATCGCCAGTAAGGGGCGT‑GTTTTTTTTATTGTTGTATTGTTGATTTGAGATTTAG‑CCCTTTGGATTGGAAATAAAAAAAAAACCGCGt > 2:104471/1‑139 (MQ=255) aTTCCAATGCTGATGACGGACAGAACGATTAAAATCACGGCCAATATGAGTATCGCCAGTAAGGGGCGT‑GTTTTTTTTTTGGTTGTTTTTTTTATTTTAGGTTTAT‑GCCGTTGCGTGGCAAAAATAAGaaaaaaaccgg > 1:124399/1‑133 (MQ=255) aTTCCAATGCTGATGACGGACAGAACGATTAAAATCACGGCCAATATGAGTATCGCCAGTAAGGGGCGT‑GGGTTTTTTTTGGTTTGTTTTTTGATTTCAGGTTTTG‑ACCGGTCCATGCCGAAAAAAATAAAAACggagg > 2:110316/1‑136 (MQ=255) aCAGAACGATTAAAATCACGGCCAATATGAGTATCGCCAGTAAGGGGCGT‑TTTTGTTTTCTGGTTGTTTTCTTCATTTCAGGTTTCG‑CCCTTTCCTTGCCAAATATAAGAAAAACGGCGTTCCGATAATCGCGGTGACa < 7:251014/139‑1 (MQ=255) aTCACGGCCAATATGAGTATCGCCAGTAAGGGGCGT‑GTTTGTTTTTTGGTTTTTTTGTTGATTTTAGGTTTAG‑GCCGTTAGGTTGCGAATATAAGAAAAAATGCGTTGCGGTAATAGCGGGGACAATGCCGccccgggc > 6:23757/1‑134 (MQ=255) aTCACGGCCAATATGAGTATCGCCAGTAAGGGGCGT‑GGTTGTTTTGGGGGTTTTTTGTTAAATTGACGTGTGG‑ACCATTCCAGTCGAAATAAAAAAAAAACCGCGTGCCGAGAATCTGGTAGCCAATTCGGACCGGTGa > 2:19319/1‑139 (MQ=255) aCGGCCAATATGAGTATCGCCAGTAAGGGGCGT‑GTTTGTTTTATGGTTTTTTTGTTAATTTAAGGTTTGG‑GCCTTTCAGTTCCAAAAAAAATAAAAACGGCGGTAAGATAATAGCGGGGACCATTCCGCCCCGTGAtt > 8:113766/1‑138 (MQ=255) gCCAATATGAGTATCGCCAGTAAGGGGCGT‑TTTTGTTTTCTGGTTGTTTTCTTCATTTCAGGTTTCG‑CCCTTTCCTTGCCAAATATAAGAAAAACGGCGTTCCGATAATCGCGGTGACAATGCCGACCGGTGATTCATa < 7:113766/139‑1 (MQ=255) gCCAATATGAGTATCGCCAGTAAGGGGCGT‑TTTTGTTTTCTGGTTGTTTTCTTCATTTCAGGTTTCG‑CCCTTTCCTTCCCAAATATAAGAAAAACGGCGTTCCGATAATCGCGGTGACAATGCCGACCGGTGATTCATa < 5:341036/139‑1 (MQ=255) tatatGAGTATCGCCAGAAAGGGGCGT‑TTTTGTTTTCTGGTTGTTTACTTCATTTCAGGTTTCG‑CCCTTTCCTTGCCAAATAAAAGAAAAACGCCGTACCGATAATCGCGGTGACAATGCCGACCGGTGATTCATAAgg < 1:104471/138‑1 (MQ=255) aaTATGAGTATCGCCAGTAAGGGGCGT‑TTTTGTTTTCTGGTTGTTTTCTTCATTTCAGGTTTCG‑CCCTTTCCTTGCCAAATATAAGAAAAACGGCGTTCCGATAATCGCGGTGACAATGCCGACCGGTGATTCATAAgg < 1:110316/139‑1 (MQ=255) tATCGCCAGTAAGGGGCGT‑TTTTGTTTTCTGGTTGTTTTCTTCATTTCAGGTTTCG‑CCCTTTCCTTGCCAAATATAAGAAAAACGGCGTTCCGATAATCGCGGTGACAATGCCGACCGGTGATTCATAAGGAAATGCaa < 2:328563/139‑1 (MQ=255) gacagCCAGTAAGGGGCGT‑TTTTGTTTTCTGGTTGTTTTCTTCATTTCAGGTTTCG‑CCCTTTCCTTGCCAAATATAAGAAAAACGGCGTTCCGATAATCGCGGTGACAATGCCGACCTGTGATTCATAAGGAAATGCaa < 7:285395/135‑1 (MQ=255) ccAGTTAGGGGCGT‑TTTTGTTTTCTGGTTGTTTTCTTCATTTCAGGTTTCG‑CCCTTTCCTTGCCAAATATAAGAAAAACGGCGTTCCGATAATCGCGGTGACAATGCCGACCGGTGATTCATAAGGAAATGCAATCCAt < 5:365739/139‑1 (MQ=255) ccAGTAAGGGGGGT‑TTTTGTTTTCTGGTTGTTTTCTTCATTTCAGGTTTCG‑CCCTTTCCTTCCAAAATATAAGAAAAACGGCGTTCCGATAATCGCGGTGACAATGCCGACCGGTGATTCATAAGGAAATGCAATCCAt < 5:38573/139‑1 (MQ=255) gggCGT‑TTTTGTTTTCTTGTTGTTTTCTTCATTTAAGTGTTCG‑CCCTTTCCTTGCCAAATATAAGAAAAACGGCGTTCCGATAATCGCGGTGACAATGCCGACCGGTGATTCATAAGGAAATGCAATCCATCTGGCCAg < 3:354662/139‑1 (MQ=255) ggCGT‑TTTTGTTTTCTGGTTGTGTTCTTCATTTCAGGTTTCG‑CCCTTTCCTTGCCAAATATAAGAAAAACGGCGTTCCGATAATCGCGGTGACAATGCCGACCGGTGATTCATAAGGAAATGCAATCCATCTGGCCAGa < 1:404578/139‑1 (MQ=255) t‑ttttGTTTTTTGGTTGGTTTCTTCATTTCAGGTTTCG‑CCCTTTCCTTGCCAAATATAAGAAAAACGGCGTTCCGATAATCGCGGTGACAATGCCGACCGGTGATTCATAAGGAAATGCAATCCATCTGGCCAGAACAt < 3:112707/139‑1 (MQ=255) t‑ttttGTTGTCGGGTTGTTTTCTGCATTTCCGGTTTCG‑CCCTTTCCTTGCCAAATATAAGAAAAACGGCGTTCCGATAATCGCGGTGACAATGCCGACCGGTGATTCATAAGGAAATGCAATCCATCTGGCCAGAACAt < 6:50362/139‑1 (MQ=255) tGGTTGTTTTCTTCATTTCAGGTTTCG‑CCCTTTCCTTGCCAAATATAAGAAAAACGGCGTTCCGATAATCGCGGTGACAATGCCGACCGGTGATTCATAAGGAAATGCAATCCATCTGGCCAGAACATCTGCGTACAcc > 4:103224/1‑139 (MQ=255) gttgttTTGTTATTTTCCGGTGGGC‑CCCTTTCGTTCCAAATTATAAGAAAAAGGGCGTTCCGATATTCGCGGTGACAATGCCGACCGGTGAGTCATAAGGAAATGCAATCCATCTGGCCAGAACATCTGCGTACACCAg < 5:247748/139‑1 (MQ=255) gttTTCTTCATTTCAGGTTTCG‑CCCTTTCCTTGCCAAATATAAGAAAAACGGCGTTCCGATAATCGCGGTGACAATGCCGACCGGTGATTCATAAGGAAATGCAATCCATCTGGCCAGAACATCTGCGTACACCAGCa > 8:65555/1‑138 (MQ=255) gatatcttcAATTCAGGTTTCG‑AACTATCCATGACAAATATAAGAAAAACGGCGTTCCGATAATCGCTTAGACAATGCCGAACGGTGATTCATTAAGATTTGCAATCTATCTGGACAGAACATCTGCGTACACCAGCa > 4:177125/5‑138 (MQ=255) ttcttcATTTCAGGTTTCG‑CCCTTTCCTTGCCAAATATAAGAAAAACGGCGTTCCGATAATCGCGGTGACAATGCCGACCGGTGATTCATAAGGAAATGCAATCCATCTGGCCAGAACATCTGCGTACACCAGCaaaa < 6:387035/138‑1 (MQ=255) ttcttcATTTCAGGTTTCG‑CCCTTTCCTTGCCAAATATAAGAAAAACGGCGTTCCGATAATCGCGGTGACAATGCCGACCGGTGATTCATAAGGAAATGCAATCCATCTGGCCAGAACATCTGCGTACACCAGCaaaa < 1:50018/138‑1 (MQ=255) ttcttcATTTCAGGTTTCG‑CCCTTTCCTTGCCAAATATAAGAAAAACGGCGTTCCGATAATCGCGGTGACAATGCCGACCGGTGATTCATAAGGAAATGCAATCCATCTGGCCAGAACATCTGCGTACACCAGCaaaa < 3:382508/138‑1 (MQ=255) ttcttcATTTCAGGTTTCG‑CCCTTTCCTTGCCAAATATAAGAAAAACGGCGTTCCGATAATCGCGGTGACAATGCCGACCGGTGATTCATAAGGAAATGCAATCCATCTGGCCAGAACATCTGCGTACACCAGCAAAAt > 8:258138/1‑139 (MQ=255) cttcATTTCAGGGTTCG‑CCCTTTCCTTGCCAAATATAAGAAAAACGGCGTTCCGATAATCGCGGTGACATTGCCGACCGGTGATTCATAAGGAAATGCAATCCATCTGGCCAGAACATCTGCGTACACCAGCAAAATg < 6:403431/138‑1 (MQ=255) cATTTCAGGTTTCG‑CCCTTTCCTTGCCAAATATAAGAAAAACGGCGTTCCGATAATCGCGGTGACAATGCCGACCGGTGATTCATAAGGAAATGCAATCCATCTGGCCAGAACATCTGCGTACACCAGCAAAATGGCa < 2:318486/138‑1 (MQ=255) cATTTCAGGTTTCG‑CCCTTTCCTTGCCAAATATAAGAAAAACGGCGTTCCGATAATCGCGGTGACAATGCCGACCGGTGATTCATAAGGAAATGCAATCCATCTGGCCAGAACATCTGCGTACACCAGCAAAATGGCa < 3:93239/138‑1 (MQ=255) ttCAGGTTTCG‑CCCTTTCCTTGCCAAATATAAGAAAAACGGCGTTCCGATAATCGCGGTGACAATGCCGACCGGTGATTCATAAGGAAATGCAATCCATCTGGc < 2:262587/104‑1 (MQ=255) ttCAGGTTTCG‑CCCTTTCCTTGCCAAATATAAGAAAAACGGCGTTCCGATAATCGCGGTGACAATGCCGACCGGTGATTCATAAGGAAATGCAATCCATCTGGc > 1:262587/1‑104 (MQ=255) ccggTTTCG‑CCCTTCCCTTCCCAAATAAACCAAAACCGGCGGCCCGAAAATCGCGGTCAAAATCCCGACGGGTGATTCAAACGCAAATGCAATCCATCTGGCCAGAACATCTGCGTACCCCCGAAAAATGCCACCGac < 6:316534/136‑2 (MQ=255) ggTTTCG‑CCCTTTCCTTGCCAAATATAAGAAAAACGGCGTTCCGATAATCGCGGTGACAATGCCGACCGGTGATTCATAAGGAAATGCAATCCATCTGGCCAGAACATCTGCGTACACCAGCAAAATGGCACCGAACAg < 3:103224/139‑1 (MQ=255) gTTTCG‑CCCTTTCCTTGCCAAATATAAGAAAAACGGCGTTCCGATAATCGCGGTGACAATGCCGACCGGTGATTCATAAGGAAATGCAATCCATCTGGCCAGaa > 3:362544/1‑104 (MQ=255) gTTTCG‑CCCTTTCCTTGCCAAATATAAGAAAAACGGCGTTCCGATAATCGCGGTGACAATGCCGACCGGTGATTCATAAGGAAATGCAATCCATCTGGCCAGaa < 4:362544/104‑1 (MQ=255) tCG‑CCCTTTCCTTGCCAAATATAAGAAAAACGGCGTTCCGATAATCGCGGTGACAATGCCGACCGGTGATTCATAAGGAAATGCAATCCATCTGGCCAGAACATCTGCGTACACCAGCAAAATGGCACCGAACAGTGcc > 7:79846/1‑139 (MQ=255) tCG‑CCCTTTCCTTGCCAAATATAAGAAAAACGGCGTTCCGATAATCGCGGTGACAATGCCGACCGGTGATTCATAAGGAAATGCAATCCATCTGGCCAGAACATCTGCGTACACCAGCAAAATGGCACCGAACAGTGcc > 8:383579/1‑139 (MQ=255) cccTTTACTTGCCAAAAAAAAGAAAAACGGCGTTCCGATAATCGCGGTGACAATGCCGACCGGTGATTCATAAGGAAATGCAATCCATCTGGCCAGAACATCTGCGTACACCAGCAAAATGGCACCGAACAGTGCCGaa < 2:371017/139‑1 (MQ=255) cTTTCCTTGCCAAATATAAGAAAAACGGCGTTCCGAGAATCGCGGTGACAATGCCGACCGGTGATTCATAAGGAAATGCAATCCATCTGGCCAGAACATCTGCGTACACCAGCAAAATGGCACCGAACAGTGCCGaaaa < 5:65531/139‑1 (MQ=255) tCCTTGCCAAATATAAGAAAAACGGCGTTCCGATAATCGCGGTGACAATGCCGACCGGTGATTCATAAGGAAATGCAATCCATCTGGCCAGAACATCTGCGTACACCAGCaaaa > 3:156937/1‑114 (MQ=255) tCCTTGCCAAATATAAGAAAAACGGCGTTCCGATAATCGCGGTGACAATGCCGACCGGTGATTCATAAGGAAATGCAATCCATCTGGCCAGAACATCTGCGTACACCAGCaaaa < 4:156937/114‑1 (MQ=255) tCCTTGCCAAATACAAGAAAAACGGCGTTCCGATAATCGCGGTGACAATGCCGACCGGTGATTCATAAGGAAATGCAATCCATCTGGCCAGAACATCTGCGTACACCAGCAAAATGGCACCGAACAGTGCCGAAAACg < 5:228195/138‑1 (MQ=255) tCCTTGCCAAATAAAAGAAAAACGGCGTTCCGATAATCGCGGTGACAATGCCGACCGGTGATTCATAAGGAAATGCAATCCATCTGGCCAGAACATCTGCGTACACCAGCAAAATGGCACCGAACAGTGCCGAAAACg < 2:124399/138‑1 (MQ=255) gCCAAATATAAGAAAAACGGCGTTCCGATAATCGCGGTGACAATGCCGACCGGTGATTCATAAGGAAATGCAATCCATCTGGCCAGAACATCTGCGTACACCAGCAAAATGGCACCGAACAGTGCCGAAAACGGAAGCa > 4:102995/1‑139 (MQ=255) ccAAATATAAGAAAAACGGCGTTCCGATAATCGCGGTGACAATGCCGACCGGTGATTCATAAGGAAATGCAATCCATCTGGCCAGAACATCTGCGTACACCAGCAAAATGGCACCGAACAGTGCCGAAAACGGAAGCa < 3:293165/138‑1 (MQ=255) ccAAATATAAGAAAAACGGCGTTCCGATAATCGCGGTGACAATGCCGACCGGTGATTCATAAGGAAATGCAATCAATCTGGCCAGAACATCTGCGTACACCAGCAAAATGGCACCGAACAGTGCCGAAAACGGAAGCAc > 5:304667/1‑139 (MQ=255) catatatAAGAAAAACGGCGTTCCGATAATCGCGGTGACAATGCCGACCGGTGATTCATAAGGAAATGCAATCCATCTGGCCAGAACATCTGCGTACACCAGCAAAATGGCACCGAACAGTGCCGAAATCGGAAGCACg < 1:19319/136‑1 (MQ=255) cAAATATAAGAAAAACGGCGTTCCGATAATCGCGGTGACAATGCCGACCGGTGATTCATAAGGAAATGCAATCCATCTGGCCAGAACATCTGCGTACACCAGCAAAATGGCACCGAACAGTGCCGAAAACGGAAGCACg < 7:258138/139‑1 (MQ=255) cAAATATAAGAAAAACGGCGTTCCGATAATCGCGGTGACAATGCCGACCGGTGATTCATAAGGAAATGCAATCCATCTGGCCAGAACATCTGCGTACACCAGCAAAATGGCACCGAACAGTGCCGAAAACGGAAGCACg < 7:378359/139‑1 (MQ=255) cAAAAATAAGAAAAACGGCGTTCCGATAATCGCGGTGACAATGCCGACCGGTGATTCATAAGGAAATGCAATCCATCTGGCCAGAACATCTGCGTACACCAGCAAAATGGCACCGAACAGTGCCGAAAACGGAAGCACg < 5:23757/139‑1 (MQ=255) atatAAGAAAAACGGCGTTCCGATAATCGCGGTGACAATGCCGACCGGTGATTCATAAGGTAATGCAATCCATCTGGCCAGAACATCTGCGTACACCAGCAAAATGGCACCGAACAGTGCCGAAAACGGAAGCACGTAt < 8:4642/139‑1 (MQ=255) | TAATGCACCGATTCCAATGCTGATGACGGACAGAACGATTAAAATCACGGCCAATATGAGTATCGCCAGTAAGGGGCGT‑TTTTGTTTTCTGGTTGTTTTCTTCATTTCAGGTTTCG‑CCCTTTCCTTGCCAAATATAAGAAAAACGGCGTTCCGATAATCGCGGTGACAATGCCGACCGGTGATTCATAAGGAAATGCAATCCATCTGGCCAGAACATCTGCGTACACCAGCAAAATGGCACCGAACAGTGCCGAAAACGGAAGCACGTAT > CP006881/787647‑787916 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |