Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 800,411 C→T 6.8% T242M (ACG→ATG)  U712_03875 → Nitric oxide synthase oxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP006881800,4110CT 6.8% 186.2 / 2.9 59T242M (ACG→ATG) U712_03875Nitric oxide synthase oxygenase
Reads supporting (aligned to +/- strand):  ref base C (34/21);  new base T (2/2);  total (36/23)
Fisher's exact test for biased strand distribution p-value = 6.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.42e-01

AATCACACATCCGGACATCGAGGCGTTTTCTGATTTGGAGCTGAAGTGGTACGGCGTGCCTATTATTTCTGATATGAAGCTTGAGGTCGGGGGCATTCATTATAATGCCGCGCCATTTAACGGCTGGTATATGGGCACGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCTGATTACAATACGGATTTATGGAAGGATCAAGCGCTAGTTGAATTGAATAAAGCTGT  >  CP006881/800274‑800549
                                                                                                                                         |                                                                                                                                          
aaTCACACATCCGGACCTCGAGGCGTTTTCTGTTTTGGAGCTGAAGTGGTACGGCGTGCCTATTATTTCTGATATGAAGCTTGAGGTCGGGGGCATTCATTATAATGCCGCGCCATTTAACGGCTGGTATATGGGCACg                                                                                                                                           <  3:209788/139‑1 (MQ=255)
     cacaTCCGGACATCGAGGCGTTTTCTGATTTGGAGCTGAAGTGGTACGGCGTGCCTATTATTTCTGATATGAAGCTTGAGGTCGGGGGCATTCATTATAATGCCGCGCCATTTAACGGCTGGTATATGGGCACGGAGAt                                                                                                                                      <  1:98430/139‑1 (MQ=255)
                      gCGTTTTCTGATTTGGAGCTGAAGTGGTACGGCGTGCCTATTATTTCTGATATGAAGCTTGAGGTCGGGGGCATTCATTATAATGCCGCGCCATTTAACGGCTGGTATATGGGCACGGAGATCGGAGCGAGAAACCTCg                                                                                                                     <  1:30589/139‑1 (MQ=255)
                                aTTTGGAGCTGAAGTGGTACGGCGTGCCTATTATTTCTGATATGAAGCTTGAGGTCGGGGGCATTCATTATAATGCCGCGCCATTTAACGGCTGGTATATGGGCACGGAGATCGGAGCGAGAAACCTCGCAGATGaa                                                                                                             >  2:309013/1‑137 (MQ=255)
                                 tttGGAGCTGAAGTGGTACGGCGTGCCTATTATTTCTGATATGAAGCTTGAGGTCGGGGGCATTCATTATAATGCCGCGCCATTTAACGGCTGGTATATGGGCACGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAg                                                                                                          <  3:412658/139‑1 (MQ=255)
                                     gAGCTGAAGTGGTACGGCGTGCCTATTATTTCTGATATGAAGCTTGAGGTCGGGGGCATTCATTATAATGCCGCGCCATTTAACGGCTGGTATATGGGCACGGAGATCGGa                                                                                                                                  >  4:130616/1‑111 (MQ=255)
                                     gAGCTGAAGTGGTACGGCGTGCCTATTATTTCTGATATGAAGCTTGAGGTCGGGGGCATTCATTATAATGCCGCGCCATTTAACGGCTGGTATATGGGCACGGAGATCGGa                                                                                                                                  <  3:130616/111‑1 (MQ=255)
                                                 tACGGCGTTCCTTTTTTTTCTGATATGAAGCTTGAGGTGGGGGGCTTTCATTATAATGCCGCGCCATTTATCGGCTGGTATATGGGCACGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCa                                                                                          <  3:408011/139‑1 (MQ=255)
                                                      cGTGCCTATTATTTCTGATATGAAGCTTGAGGTCGGTGGCATTCATTATAATGCCGCGCCATTTAACGGCTGGTATATGGGCACGTAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCaaaa                                                                                       >  2:267383/1‑137 (MQ=255)
                                                        tGCCTATTATTTCTGATATGAAGCTTGAGGTCGGGGGCATTCATTATAATGCCGCGCCATTTAACGGCTGGTATATGGGCACGGAGATCGGAGCGAGAAACCTCGCAGATGATAAGCGGTACGACAAGCTCaaaaaat                                                                                    >  5:90731/1‑137 (MQ=255)
                                                                   tCTGATATGAAGCTTGAGGTCGGGGGCATTCATTATAATGCCGCGCCATTTAACGGCTGGTATATGGGCACGGAGATCTGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGa                                                                        >  7:259017/1‑139 (MQ=255)
                                                                   tCTGATATGAAGCTTGAGGTCGGGGGCATTCATTATAATGCCGCGCCATTTAACGGCTGGTATATGGGCACGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGa                                                                        >  4:142517/1‑139 (MQ=255)
                                                                       atatGAAGCTTGAGGTCGGGGGCATTCATTATAATGCCGCGCCATTTAACGGCTGGTATATGGGCACGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCgg                                                                    >  5:169762/1‑139 (MQ=255)
                                                                       atatGAAGCTTGAGGTCGGGGGCATTCATTATAATGCCGCGCCATTTAACGGCTGGTATATGGGCACGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCgg                                                                    >  4:146181/1‑139 (MQ=255)
                                                                                 tGAGGTCGGGGGCATTCATTATAATGCCGCGCCATTTAACGGCTGGTATATGGGCACGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCt                                                          >  7:303802/1‑139 (MQ=255)
                                                                                 tGAGGTCGGGGGCATTCATTATAATGCCGAGCCATTTAACGGCTGGTATATGGGCACGGAGATCGGAGCGAGAAAACTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCt                                                          >  7:332092/1‑139 (MQ=255)
                                                                                  gggggcGGGGGCATCAATTATATTGCCGCGCCATTAAACGGCTGGTATATGGGCACGGAGATCGGAGCGAGAAACCTCGCAGATCAAAAGCGGTCCGAAACGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCt                                                          <  4:246119/133‑1 (MQ=255)
                                                                                        gggggCATTCATTATAATGCCGCGCCATTTAACGGCTGGTATATGGGCATGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCTGATTAc                                                    >  5:381156/1‑138 (MQ=255)
                                                                                        gggggCATTCATTATAATGCCGCGCCATTTAACGGCTGGTATATGGGCATGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCTGATTAc                                                    >  5:387009/1‑138 (MQ=255)
                                                                                        gggggCATTCATTATAATGCCGCGCCATTTAACGGCTGGTATATGGGCACGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCTGATTAc                                                    >  3:102506/1‑138 (MQ=255)
                                                                                           ggacttcattATAAGGCCGCGACATTTAAGGGCTGGTAAATGGGCCCGGAGAGCGGAGCGAGAAACCTCGCAGTTGAAAAGCGGTACGACAAGGTCAAAAATGTAGCGTCCGTGATCGGCATCGCCGCTGATTACAATa                                                <  5:245785/135‑1 (MQ=255)
                                                                                           ggCATTCATTATAATGCCGCGCCATTTAACGGCTGGTATATGGGCATGGAGATCGTAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCTGATTACAATa                                                <  6:381156/139‑1 (MQ=255)
                                                                                           ggCATTCATTATAATGCCGCGCCATTTAACGGCTGGTATATGGGCATGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCTGATTACAATa                                                <  6:387009/139‑1 (MQ=255)
                                                                                           ggCATTCATTATAATGCCGCGCCATTTAACGGCTGGTATATGGGCACGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCTGATTACAATa                                                <  4:102506/139‑1 (MQ=255)
                                                                                              attcattATCATCCCGCGCCATTTAACGGCGGGTATATGGGCACGGAGAGCGGCGCGATAAACCTCGAAGATGAAAAGCGGAACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCTGATTACAATACgg                                             <  8:313833/139‑1 (MQ=255)
                                                                                                       aaTGCCGCGCCATTTAACGGCTGGTATATGGGCACGGAGATCGGAGCGAGAACCCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCTGATTACAATACGGAGTTATg                                      >  7:152215/1‑137 (MQ=255)
                                                                                                        aTGCGGGGCCATTTATCGGGTGTTATTTGGGCACGGAGTTCGCAGCTAGAAACCTGGCAGATTAAAAGGGGTACGACAAGCTAAAAAATGTCGCGTCCGTGATCGGCATCGCCGCTGATTACAATACGGATTTATGGa                                    <  1:309013/138‑1 (MQ=255)
                                                                                                         tGCCGCGCCATTTAACGGCTGGTATATGGGCACGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCTGATTACAATACGGATTTATGGAAg                                  >  4:132015/1‑139 (MQ=255)
                                                                                                          gCCGCGCCATTTAACGGCTGGTATATGGGCACGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCTGATTACAATACGGATTTATGGAAg                                  >  5:65757/1‑138 (MQ=255)
                                                                                                           ccGCGCCATTTAACGGCTGGTATATGGGCACGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCGGATTACAATACGGATTTATGGAAGGa                                >  1:165180/1‑139 (MQ=255)
                                                                                                            cgcgCCATTTAACGGCTGGTTTATGGGCACGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCTGATTACAATa                                                <  3:156210/122‑1 (MQ=255)
                                                                                                            cgcgCCATTTAACGGCTGGTATATGGGCACGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATcgccg                                                            >  6:213325/1‑110 (MQ=255)
                                                                                                            cgcgCCATTTAACGGCTGGTATATGGGCACGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATcgccg                                                            <  5:213325/110‑1 (MQ=255)
                                                                                                            cgcgCCATTTAACGGCTGGTATATGGGCACGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCTGCTTACAATACGGATTTATGGAAGGAt                               >  1:208614/1‑139 (MQ=255)
                                                                                                            cgcgCCATTTAACGGCTGGTATATGGGCACGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCTGATTACAATa                                                >  4:156210/1‑122 (MQ=255)
                                                                                                            cgcgCCATCTAACGGCTGGTATATGGGCACGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCTGATTACAATACGGATTTATGGAAGGa                                >  4:283133/1‑138 (MQ=255)
                                                                                                              cgcCATTTAACGGCTGGTATATGGGCACGGAGATCGGAGCGAGAAACCTCg                                                                                                                     <  6:191910/51‑1 (MQ=255)
                                                                                                              cgcCATTTAACGGCTGGTATATGGGCACGGAGATCGGAGCGAGAAACCTCg                                                                                                                     >  5:191910/1‑51 (MQ=255)
                                                                                                              cgcCATTTAACGGCTGGTATATGGGCACGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCTGATTACAATACGGATTTATGGAAGGATc                              >  8:379587/1‑138 (MQ=255)
                                                                                                              cgcCATTTAACGGCTGGTATATGGGCACGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCTGATTACAATACGGATTTATGGAAGGATc                              >  7:74853/1‑138 (MQ=255)
                                                                                                              cgcCATTTAACGGCTGGTATATGGGCACGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCTGATTACAATACGGATTTATGGAAGGATc                              >  1:236331/1‑138 (MQ=255)
                                                                                                              cgcCATTTAACGGCTGGTATATGGGCACGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCTGATTACAATACGGATTTATGGAAGGATc                              >  3:36744/1‑138 (MQ=255)
                                                                                                              cgcCATTTAACGGCTGGTATATGGGCACGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGAATCGCAGCTGATTACAATACGGATTTATGGAAGGATc                              >  7:220342/1‑138 (MQ=255)
                                                                                                              cgcCATTTAACGGCTGGTATATGGGCACGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGAAGCGTCCGTGATCGGCATCGCCGCTGAGTACAATACGGATTTATGGaactgtct                             >  1:168098/1‑133 (MQ=255)
                                                                                                                   tttAACGGCTGGTATATGGGCACGGAGATCGGAGCGAGAACGCTCGCAGATGAAAAGCGGTACCACAACCTCAAAAAAGTAGCGTCCGTGGTCGGCATCGGCGCGGATTACAATTCGGATTTATCGATGGATCAAgcgc                        >  6:142597/1‑139 (MQ=255)
                                                                                                                       aCGGCTGGTATATGGCCACGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGTAACGACAAGCTAAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCTGATTACAATACGGATTTATGGAAGGATCAAGCGCTAg                     <  3:146181/138‑1 (MQ=255)
                                                                                                                           cTGGTATATGGGCACGGATTTCGGTGCTATAAACCTCGCAGATGAAAAGCGTTACGACAAGCTCAATAAAGTAGCGTCCGTGATCGGCATCGCCGCTGATTACAATACGGATTTATGGAAGGATCAAGCGCTAGttgaa                <  8:220342/139‑1 (MQ=255)
                                                                                                                             ggTATATGGGCACGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCTGCTTCGCCGCTGATTACAATACGGATTTATGGAAGGATAAAGCGCTAGttgattt              >  3:241138/1‑139 (MQ=255)
                                                                                                                             ggTATATGGGCACGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCTGATTACAATACGGATTTATGGAAGGATCAAGCGCTAGttgaatt              >  4:128236/1‑139 (MQ=255)
                                                                                                                                 tatGGGCACGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACACGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCTGATTACAATACGGATTTATGGAAGGATCAAGCGCTAGttgaattgaa           >  3:360694/1‑138 (MQ=255)
                                                                                                                                   tGGGCACGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAGAAAGTAGCGTCCGTGATCGGCATCGCCGCTGATTACAATACGGATTTATGGAAGGATCAAGCGCTAGTTGAATTGAATa         <  4:155298/138‑1 (MQ=255)
                                                                                                                                   tGGGCACGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGACGCTGATTACAATACGGATTTATGGAAGGATCAAGCGCTAGTTGAATTGAATa         >  4:174477/1‑138 (MQ=255)
                                                                                                                                     ggCACGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCgg                                                                    <  6:304472/77‑1 (MQ=255)
                                                                                                                                     ggCACGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCgg                                                                    >  5:304472/1‑77 (MQ=255)
                                                                                                                                     ggCACGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCTGATTACAATACGGATTTATGGAAGGATCAAgcgc                        >  8:234081/1‑121 (MQ=255)
                                                                                                                                     ggCACGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCTGATTACAATACGGATTTATGGAAGGATCAAgcgc                        <  7:234081/121‑1 (MQ=255)
                                                                                                                                     ggCACGGAGAGCGGCGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCTGATTACAATACGGATTTATGGAAGGATCAAGCGCTAGTTGAATTGAATAAAg      <  5:174630/139‑1 (MQ=255)
                                                                                                                                        aCGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCTGATTACAATACGGATTTATGGAAGGATCAAGCGCTAGttgaa                >  8:36939/1‑126 (MQ=255)
                                                                                                                                        aCGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCTGATTACAATACGGATTTATGGAAGGATCAAGCGCTAGttgaa                <  7:36939/126‑1 (MQ=255)
                                                                                                                                         cGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCa                                                                                          >  4:314680/1‑51 (MQ=255)
                                                                                                                                         cGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCa                                                                                          <  3:314680/51‑1 (MQ=255)
                                                                                                                                         cGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCAt                                                                 >  7:210879/1‑76 (MQ=255)
                                                                                                                                         cGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCAt                                                                 <  8:210879/76‑1 (MQ=255)
                                                                                                                                         cGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCTGATTACAATTCGGATTTATGGAAGGATCAAGCGCTAGTTGAATTGAATAAAGCtgt  >  3:139497/1‑139 (MQ=255)
                                                                                                                                         cGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCTGATTACAATACGGATTTATGGAAGGATCAAGCGCTAGTTGAATTGAATAAAg      <  5:205235/135‑1 (MQ=255)
                                                                                                                                         cGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCTGATTACAATACGGATTTATGGAAGGATCAAGCGCTAGTTGAATTGAATAAAg      >  6:205235/1‑135 (MQ=255)
                                                                                                                                         cGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCTGATTACAATACGGATTTATGGAAGGATCAAGCGCTAGTTGAATTGAATAAAGCtgt  >  3:8113/1‑139 (MQ=255)
                                                                                                                                         cGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCTGATTACAATACGGATTTATGGAAGGATCAAGCGCTAGTTGAATTGAATAAAGCtgt  >  2:323704/1‑139 (MQ=255)
                                                                                                                                         cGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCTGATTACAATACGGATTTATGGAAGGATCAAGCGCTAGTTGAATTGAATAAAGCtgt  >  7:110663/1‑139 (MQ=255)
                                                                                                                                         cGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCTGATTACAATACGGATTTATGGAAGGATCAAGCGCTAGTTGAATTGAATAAAGCtgt  >  2:173658/1‑139 (MQ=255)
                                                                                                                                         cGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCTGATTACAATACGGATTTATGGAAGGATCAAGCGCTAGTTGAATTGAATAAAGCtgt  >  1:79873/1‑139 (MQ=255)
                                                                                                                                         cGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCTGATTACAATACGGATTTATGGAAGGATCAAGCGCTAGTTGAATTGAATAAAGCtgt  >  3:236529/1‑139 (MQ=255)
                                                                                                                                         cGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCTGATTACAATACGGATTTATGGAAGGATCAAGCGCGAGTTGAATTGAATAACGCtgt  >  2:308344/1‑139 (MQ=255)
                                                                                                                                         cGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCTGATTACAATACGGATTTATGGAAGGATCAACCGCTAGTTGAATTGAATAAAGCtgt  >  1:184726/1‑139 (MQ=255)
                                                                                                                                         cGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCTGATTACAATACGGATATATGGAAGGATCAAGCGCTAGTTGAATTGAATAAAGCtgt  >  3:61172/1‑139 (MQ=255)
                                                                                                                                         cGGAGATCGGAGCGAGAAACATCGAAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGAACGGCATCGCCGCTGATTACAATACGGATTTATGGAAGGATCAAGCGCTAGTTGAATTGAATATAGCtgt  >  8:185239/1‑139 (MQ=255)
                                                                                                                                         |                                                                                                                                          
AATCACACATCCGGACATCGAGGCGTTTTCTGATTTGGAGCTGAAGTGGTACGGCGTGCCTATTATTTCTGATATGAAGCTTGAGGTCGGGGGCATTCATTATAATGCCGCGCCATTTAACGGCTGGTATATGGGCACGGAGATCGGAGCGAGAAACCTCGCAGATGAAAAGCGGTACGACAAGCTCAAAAAAGTAGCGTCCGTGATCGGCATCGCCGCTGATTACAATACGGATTTATGGAAGGATCAAGCGCTAGTTGAATTGAATAAAGCTGT  >  CP006881/800274‑800549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑