BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	915,787	T→G	13.4%	noncoding (53/72 nt)	U712_21220 →	tRNA‑Gly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	915,787	0	T	G	13.4%	143.8 / 6.7	84	noncoding (53/72 nt)	U712_21220	tRNA‑Gly
Reads supporting (aligned to +/- strand): ref base T (38/34); new base G (8/4); total (46/38)
Fisher's exact test for biased strand distribution p-value = 5.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGTCATGCGTTGGTTCGAATCCAGCTAGCCCAGTCTCACTTTTTATATCAACTGCATATAATGTATTAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAGTGGTAAGGCAGAGGTCTGCAAAACCTTTATCCCCGGTTCGAATCCGGGTGTCGCCTTCTTATTGCCGGGGTGGTGGAATTGGCAGACACACA  >  CP006881/915652‑915921

ccGTCATGCGTTGGTTCGAATCCAGCTAGCCCAGTCTCACTTTTTATATCAACTGCATATAATGTATTAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTGCg                                                                                                                                     >  1:199433/1‑139 (MQ=255)

 cGTCATGCGTTGGTTCGAGTCCAGCTAGCCCAGTCTCAGTTTTTATATCTACTGCATATATTGTATTATGTCCATTAAATATGCGGAAGTAGTTCAGTGGTAGAACACCCCCTTGCCAAGGTGGGGGTCGCGGGGTc                                                                                                                                      >  5:266574/1‑137 (MQ=25)

 cGTCATGCGTTGGTTCGAATCCAGCTAGCCCAGTCTCACTTTTTATATCAACTGCATATAATGTATTAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGACCACCACCTTGCCAAGGTGGGGGTCGCGGGTTCg                                                                                                                                     >  5:66441/1‑138 (MQ=255)

 cGTCATGCGTTGGTTCGAATCCAGCTAGCCCAGTCTCACTTTTTATATCAACTGCATATAATGTATTAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCg                                                                                                                                     >  1:47965/1‑138 (MQ=255)

 cGTCATGCGTTGGTTCGAATCCAGCTAGCCCAGTCTCACTTTTTATATCAACTGCATATAATGTATTAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGGTCGa                                                                                                                                    >  5:241797/1‑139 (MQ=255)

           tGGTTCGAATCCAGCTAGCCCAGTCTCACTTTTTATATCAACTGCATATAATGTATTAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCtt                                                                                                                          >  3:398231/1‑139 (MQ=255)

           tGGTTCGAATCCAGCTAGCCCAGTCTCACTTTTTATATCAACTGCATATAATGTATTAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCtt                                                                                                                          >  7:156/1‑139 (MQ=255)

           tGGTTCGAATCCAGCTAGCCCAGTCTCACTTTTTATATCAACTGCATATAATGTATTAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCtt                                                                                                                          >  6:163373/1‑139 (MQ=255)

            ggTTCGAATCCAGCTAGCCCAGTCTCACTTTTTATATCAACTGCATATAATGTATTAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGCATCCCGTCTTc                                                                                                                         >  8:245802/1‑139 (MQ=255)

             gTTCGAATCCAGCTAGCCCAGTCTCACTTTTTATATCAACTGCATATAATGTATTAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCAGTCTTcc                                                                                                                        >  8:116728/1‑139 (MQ=255)

                  aaTCCAGCTAGCCCAGTCTCACTTTTTATATCAACTGCATATAATGTATTAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATccc                                                                                                                               >  2:366485/1‑127 (MQ=255)

                  aaTCCAGCTAGCCCAGTCTCACTTTTTATATCAACTGCATATAATGTATTAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATccc                                                                                                                               <  1:366485/127‑1 (MQ=255)

                        gCTAGCCCAGTCTCACTTTTTATATCAACTGCATATAATGTATTAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGCATGCCGTCTTCCGCTCCAAATAc                                                                                                             >  6:135928/1‑139 (MQ=255)

                            gCCCAGTCTCACTTTTTATATCAACTGCATATAATGTATTAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTAcgg                                                                                                           <  3:207911/137‑1 (MQ=255)

                            gCCCAGTCTCACTTTTTATATCAACTGCATATAATGTATTAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTAcgg                                                                                                           <  6:66441/137‑1 (MQ=255)

                             cccAGTCTCACTTTTTATATCAACTGCATATAATGTATTAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGCATCCCGGCTTACGCACCAAATAagtcgg                                                                                                        >  6:231084/1‑139 (MQ=25)

                               cAGTCTCACTTTTTATATCAACTGCATATAATGTATTAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCa                                                                                                      <  8:295147/139‑1 (MQ=255)

                               cAGTCTCACTTTTTATATCAACTGCATATAATGTATTAAGGCCATTAAAAATGCGGAAGTAGTGCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCa                                                                                                      <  6:337964/139‑1 (MQ=255)

                                      aCTTTTTATATCAACTGCATATAATGTATTAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCAGTCTTCCGCTCCAATTACGGCGGCATAGCCa                                                                                                <  7:116728/138‑1 (MQ=255)

                                           ttATATCAACTGCATATAATGTATTAAGTCCATTAAATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCGATGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAGTgg                                                                                           <  4:52806/138‑1 (MQ=255)

                                           ttATATCAACTGCATATAATGTATTAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGGTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAGTgg                                                                                           <  6:241797/138‑1 (MQ=255)

                                           ttATATCAACTGCATATAATGTATTAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGGTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAGTgg                                                                                           <  3:59866/138‑1 (MQ=255)

                                           ttATATCAAATGCTTATAATGTATTACGTCCATTAAAAATGCGGAAGTAGTTCTGTGTTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAGTgg                                                                                           <  4:42603/138‑1 (MQ=21)

                                            tataTCAACTGCATATAATGTATTAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCa                                                                                                <  2:139233/132‑1 (MQ=255)

                                            tataTCAACTGCATATAATGTATTAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAGTGGt                                                                                          >  5:283073/1‑138 (MQ=255)

                                            tataTCAACTGCATATAATGTATTAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGGTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCa                                                                                                >  1:139233/1‑132 (MQ=255)

                                            taattCAACTGCATATAATGTATTACGTCCATTAAAAATGCTGATGTAGTTCAGTTGGAGAACACCACCTTGCCACGGTGGGGGTCGCGGGTTCGAAGCCCGTCTTCCGATCCAATTACGGCGGCATAGCCAAGTGGt                                                                                          <  3:51876/134‑1 (MQ=25)

                                             atatCAACTGCATATAATGTATTAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAGTGGTa                                                                                         <  6:72495/138‑1 (MQ=255)

                                                tCAACTGCATATAATGTATTAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAGTGGTAAGGc                                                                                     >  8:139122/1‑139 (MQ=255)

                                                 cAACTTCATATAATGTATTAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGGTCGAATCCCGTCTTCCGGTCCAATTAGGGCGGCATAGCCAAGTGGTAAGGCa                                                                                    <  3:382243/139‑1 (MQ=255)

                                                 cAACTGCATATAATGTATTAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGGGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAGTGGTAAGGCa                                                                                    <  7:183870/139‑1 (MQ=255)

                                                  caCTGCATTTATTGTATTATGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAGTGGTAAGGCag                                                                                   <  5:252060/138‑1 (MQ=255)

                                                    cTGCATATAATGTATTAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTAGAATACCGGCTTACTCAGCAAATAAGCCCGCATACCCCACTGGTAAGGCagag                                                                                 >  3:69345/1‑139 (MQ=18)

                                                       cATATAATGTATTAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAGTGGTAAGGCAGAGGt                                                                               <  5:163373/138‑1 (MQ=255)

                                                           taATGTATTAAGTCCATTAAAAATGCGGAAGTCGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAGTGGTAAGGCAGAGGTCTGCa                                                                          <  3:57590/139‑1 (MQ=255)

                                                           taATGTATTAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAGTGGTAAGGCAGAGGTCTGCa                                                                          <  2:47965/139‑1 (MQ=255)

                                                            aaTGTATTAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGGGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAGTGGTAAGGCAGAGGTCTGCaa                                                                         <  1:66445/139‑1 (MQ=255)

                                                             aTGTATTAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAGTGGTAAGGCAGAGGTCTGCaa                                                                         <  7:139122/138‑1 (MQ=255)

                                                              tGTATTAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAGTGGTAAGGCAGAGGTCTGCaaaa                                                                       >  7:106543/1‑139 (MQ=255)

                                                                 aTTAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGtt                                                                                                                                       <  3:89329/72‑1 (MQ=255)

                                                                 aTTAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGtt                                                                                                                                       >  4:89329/1‑72 (MQ=255)

                                                                 aTTAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCa                                                                                                      >  8:3240/1‑105 (MQ=255)

                                                                 aTTAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCa                                                                                                      <  7:3240/105‑1 (MQ=255)

                                                                 aTTAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGGTCGCATCCCGTGAGCCGCGCCAATTAAGGCGGCAGAGCCAAATGGGTAGGCACAGGTCGGGAAAAcat                                                                    >  3:209767/1‑137 (MQ=18)

                                                                  ttAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAGTGGTAAGGCAGAGGTCTGCAAAACCtt                                                                   >  3:383828/1‑139 (MQ=255)

                                                                  ttAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAGTGGTAAGGCAGAGGGCTGCAAAACCtt                                                                   >  1:306468/1‑139 (MQ=255)

                                                                   tAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATACCGTCTTCCGCGCCAATTTCGGCGGCCTAGCCAAGTGGTAAGGCAGAGGTCTGGAAAACCttt                                                                  >  4:276046/1‑139 (MQ=25)

                                                                     aGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAGTGGTAAGGCa                                                                                    >  8:236860/1‑119 (MQ=255)

                                                                     aGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAGTGGTAAGGCa                                                                                    <  7:236860/119‑1 (MQ=255)

                                                                      gTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAAACCCGTATTCCGCTCCAATTACGGCGGCATAGCCAATGGGTAATGCAGAGGTCTTCAACACCTTTAta                                                               >  8:382810/1‑138 (MQ=21)

                                                                        ccATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTGGCATCCCGTCGTGCGCTCCAATTACGGCGGCATAGCCAAGTGGTAAGGGAGAGGTCGGCCAAACCTTTATcac                                                             >  4:353759/1‑137 (MQ=21)

                                                                         cATTAAAAATGCGTCAGTAGTTCAGTGGTAGTACACCACCTTGCCATGGTGGGGGTCGCGGGTTCGAATCACGTCTTCCGCTGCAATTACGGCGGCATAGACAAGTGGTAAGGCAGAGGTCTGCAAAACCTTTATccc                                                             <  4:69345/138‑1 (MQ=25)

                                                                         cATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAGTGGTAAGGCAGAGGTCTGCAAAACCTTTATccc                                                             <  8:156/138‑1 (MQ=255)

                                                                          aTTAAAAATGCGGAAGTAGTTCAGTGGTAGAACCCCACCTTGCCAAGGTGGGGGTCGCGGGGTCGAATCCCGTCTTCCGCGCCAGTTACGGCGGCCTAGCCCAGTGGTAAGGCAGAGGTCTGCAACACCTTTATCCCCg                                                           >  8:94496/1‑139 (MQ=21)

                                                                          aTTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGCCTGCCGTCTTCCCCGCCAATTTCGGCGGCAATGCCCAGTGGTTAGGCAGAGGTCTGCGAAACCATTATCCCCg                                                           >  3:117718/1‑139 (MQ=18)

                                                                          aTTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCGTCCGCTCCAATTTCGGCGGCATAGCCCAGTGGTTAGGCACAGGTCTGCAAAACCTTTATCCCCg                                                           >  7:302259/1‑139 (MQ=21)

                                                                          aTTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGGTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATTGCCAAGTGGTAAGGCAGAGGTCTGCAAAACCTTTATACCCg                                                           >  1:62412/1‑139 (MQ=255)

                                                                          aTTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGGTCGAATCCCGTCTTACGCTCCAAATACGGCGGCATAGCCAAGTGGTAAGGCAGAGGTCTGCAAAACCTTTATACCCg                                                           >  4:386345/1‑139 (MQ=255)

                                                                                 aTGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAGTGGTAAGGCAGAGGTCTGCAAAACCTTTATCCCCGGTTCGaa                                                    >  8:224923/1‑139 (MQ=255)

                                                                                    cGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTGCAATTACGGCGGCATAGCCAAGTGGTTAGGCAGAGGTCTGCAAAACCTTTATCCCCGGTTCGAATcc                                                 >  7:317404/1‑139 (MQ=255)

                                                                                    cGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAGTGGTAAGGCAGAGGTCTGCAAAACCTTTATCCCCGGTTCGAATc                                                  <  7:401980/138‑1 (MQ=255)

                                                                                    cGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCGCCAAATTCGGCCGCATAGCCAAATGGTAAGGCAGAGGGCTGCAAAACCGTTATACCCGGTTACAATcc                                                 >  3:227782/1‑139 (MQ=18)

                                                                                        agtagtTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAGTGGTAAGGCAGAGGTCTGCAAAACCTTTATCCCCGGTTCGAATCCGGgt                                             <  6:283073/139‑1 (MQ=255)

                                                                                         gtagtTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAGTGGTAAGGCAGAGGTCTGCAAAACCTTTATCCCCGGTTCGAATCCGGgtg                                            <  2:199433/139‑1 (MQ=255)

                                                                                               cattGGTAGATCACCACCTTGCGAAGGTGGGGGTCGCGGGGTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAGTGGTAAGGCAGTGGTCTGCATAAGCTTTATCCCCGGTTCGAATCCTGGTGTCGcc                                       <  3:409733/135‑1 (MQ=25)

                                                                                                  tGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAg                                                                                              <  4:252273/80‑1 (MQ=255)

                                                                                                  tGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAg                                                                                              >  3:252273/1‑80 (MQ=255)

                                                                                                       gAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAGTGGTAAGGCAGAGGTCTGCAAAACCTTTATCCCCGGTTc                                                       <  4:239022/114‑1 (MQ=255)

                                                                                                       gAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAGTGGTAAGGCAGAGGTCTGCAAAACCTTTATCCCCGGTTc                                                       >  3:239022/1‑114 (MQ=255)

                                                                                                       gAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCGCCAAATACGGCGGCATAGCCAAGTGGTAAGGCAGAGGTCTGCAAAACCTTTATCCCCCGGTCGAATCCCGGTGTCGCCCTCTTATTg                              >  8:343633/1‑139 (MQ=37)

                                                                                                          caccacCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAGTGGTAAGGCAGAGGTCTGCAAAACCTTTATCCCCGGTTCGAATCCGGGTGTCGCCTTCTTATTGCCg                           <  4:398231/139‑1 (MQ=255)

                                                                                                            ccaccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCCATTACGGCGGCATAGCCAAGTGGTAAGGCAGAGGTCTGCAAAACCTTTATCCCCGGTTCGAATCCGGGTGTAGCCTTCTTATTGCCggg                         >  8:62800/1‑139 (MQ=255)

                                                                                                            ccaccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAGTGGTAAGGCAGAGGTCTGCAAAACCTTTATccc                                                             <  3:67535/103‑1 (MQ=255)

                                                                                                            ccaccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAGTGGTAAGGCAGAGGTCTGCAAAACCTTTATccc                                                             >  4:67535/1‑103 (MQ=255)

                                                                                                            ccaccTTGCCAAGGTGGGGGTCGCGGGTTCGAATACCGTCGTCCGCTCCAATTACGGCGGCATAGCCAAGTGGTAAGGCTGAGGTATGCAAAACCTTTATCCCCGGTTCGAATACGGGGGTCTCCGTCTTATTGCAggg                         >  6:355557/1‑139 (MQ=37)

                                                                                                            ccaccTTGCCAAGGTGGGGGTCGCGGGGTCGCATCCCGGCTTCCGCTCCAATTACGGCGGCATAGCCAACTGGTAAGGGAGAGGGCTGCAAAACCTTTATCCCCGGTTCGACTCCGGGTGTCGGCGTGTGATTGcgcgg                         >  1:66048/1‑135 (MQ=25)

                                                                                                                  tGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAGTGGTAAGGCAGAGGTCTGCAAAACCTTTATCCCCGGTTCGAATCCGGGTGTCGCCTTCTTATTGCCGGGgtggtg                   >  5:327129/1‑139 (MQ=255)

                                                                                                                     cAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAGTGGTAAGGCAGAGGTCTGCAAAACCTTTATCCCCGGTTCGAATCCGGGTGTCGCCTTCTTATTGCCGGGGTGGTGGaa                <  4:381780/139‑1 (MQ=255)

                                                                                                                       aGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAGTGGTAATGCAGAGGTCTGCAAAACCATTATCCCCGGGTCGAATCCGGGTGTCGCCTTGTGTTTTCAGTGGTGGTGGGAtt              >  5:158067/1‑139 (MQ=37)

                                                                                                                       aGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAACTGGTAAGGCAGAGGTCTGCAAAACCTTTATCCCCGGTTCGAATCCGGGTGTCGCCGTCTTATTTCCGGGGGGGTGGAAtt              >  3:337232/1‑139 (MQ=255)

                                                                                                                         gTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAGTGGTAAGGCAGAGGTCTGCAAAACCTTTATCCCCGGTTc                                                       >  1:58668/1‑96 (MQ=255)

                                                                                                                         gTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAGTGGTAAGGCAGAGGTCTGCAAAACCTTTATCCCCGGTTc                                                       <  2:58668/96‑1 (MQ=255)

                                                                                                                            ggggTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAGTGGTAAGGCAGAGGTCTGCAAAACCTTTATCCCCGGTTCGAATCCGGGTGTCGCCTTCTTATTGCCGGGGTGGTGGAATTGGCAg         <  3:276046/139‑1 (MQ=255)

                                                                                                                              ggTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAGTGGTAAGGCAGAGGTCTGC‑AAACCTTTATCCCCGGTTCGAATCCGGGTGTCGCCGTCTTTTTGGCGGGGGGGTGGATTTGGCGGaca      >  3:82955/1‑139 (MQ=38)

                                                                                                                                 cgcgGGTTCGAATCCCGTCTTCCGCTCCAATTCCGGCGGCAGAGACAAGTGGTAAGGCAGAGGTCTGCAAAACCTTTATCCCCGGTTCGAATCCGGGTGTCGCCTTCTTATTGCCGGGGTGGTGGAATTGGCAGacaca    <  5:231084/139‑1 (MQ=255)

                                                                                                                                   cgGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAGTGGTAAGGCAGAGGTCTGCAAAACCTTTATCCCCGGTTCGAATCCGGGTGTCGCCTTCTTATTGCCGGGGTGGTGGAATTGGCAGAcacaca  <  4:209767/139‑1 (MQ=255)

CCGTCATGCGTTGGTTCGAATCCAGCTAGCCCAGTCTCACTTTTTATATCAACTGCATATAATGTATTAAGTCCATTAAAAATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTACGGCGGCATAGCCAAGTGGTAAGGCAGAGGTCTGCAAAACCTTTATCCCCGGTTCGAATCCGGGTGTCGCCTTCTTATTGCCGGGGTGGTGGAATTGGCAGACACACA  >  CP006881/915652‑915921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`