| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 948,353 | T→G | 23.2% | L140R (CTT→CGT) | U712_04645 → | putative lipoprotein yhcJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 948,353 | 0 | T | G | 23.2% | 69.6 / 6.7 | 41 | L140R (CTT→CGT) | U712_04645 | putative lipoprotein yhcJ |
| Reads supporting (aligned to +/- strand): ref base T (9/22); new base G (8/2); total (17/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.27e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTTAATTTGGCACCGCTCGGGACGACATACATAACGCCCATGGGCATTTATTCAAAACGATATGAACGAATCCGAGATATATCGCGGGGAGCTGTCGTTTCTGTTCCGGATAAAGCGTTCGATTTTGGCAGGGCGCTTACGGTGCTCCAGGAAGCGGGTTTGCTAACGCTGAAAAACGGATTTAACGGAACAGGTTCAGTTGATATGATTAAAGATAATCCGAGACACCTGAAATTAAAAGCTGTCCGGCAACAGGATGCAGTGAG > CP006881/948217‑948482 | cTTAATTTGGCACCGCTCGGGACGACATACCTAACGCCCCTGGGCATTTATTCAAAACGATATGAACGAATCCGAGATATATCGCGGGGAGCTGTCGTTTCTGTTCCGGATAAAGCGTTCGATTTTGGCAGGGCGCTTa < 1:303507/139‑1 (MQ=255) aaTTTGGCACCGCTCGGGACGACATACATAACGCCCATGGGCATTTATTCAAAACGATATGAACGAATCCGAGATATATCGCGGGGTGGTGTAGTTTCTGTTCCGTATAAAGCGTTCGATTTTGGCAGGGAGCTTACg < 4:238722/138‑1 (MQ=255) tttGGCACCGCTCGGGACGACATACATAACGCCCATGGGCATTTATTCAAAACGATATGAACGAATCCGAGATATATCGCGGGGAGCTGTCGTTTCTGTTCCGGATAAAGCGTTCGATTTTGGCAGGGCGCTTACGGTg < 5:38257/139‑1 (MQ=255) acatacatAACGCCCATGGGCATTTATTCAAAACGATATGAACGAATCCGAGATATATCGCGGGGAGCTGTCGTGTTTGGTGCCGCTTAACCGTGGGCTTTTGGGGGGGCGGGTTCGGTGCTGCCCGGAGCGGGGTTGc > 1:257168/1‑139 (MQ=255) catacataACGCCCATGGGCATTTATTCAAAACGATATGAACGAATCCGAGATATATCGCGGGGAGCTGACGTTTCTGGTCCGGATAAAGCGTTCGATTTTGGCAGGGCGCTTACGGTGCTCCAGGAAGCGGGTTTGc > 8:146996/1‑138 (MQ=255) gCCCATGGGCATTTATTCAAAACGATATGAACGAATCCGAGATATATCGCGGGGAGCTGTCGTTTGTGTTGGGGTTAAAGCGGTGGATTTTTGGCGGGGGCGTTCGGTGGTCCAGGTAGCGGGTTTGGTAACGCTGaa > 7:116031/1‑138 (MQ=255) gCCCATGGGCATTTATTCAAAACGATATGAACGAATCCGAGATATATCGCGGGGAGCTGTCGTGTGGGTTGCGGCTTAATCGGTAGGTTTTGGCAGGGGGCGGACGGTGGTCCAGGTTGCGGGGTTGCGTACCCTGaa > 2:116802/1‑138 (MQ=255) gCCCATGGGCATTTATTCAAAACGATATGAACGAATCCGAGATATATCGCGGGGAGCTGTCGTGTATGTTGCGGATTAAAAGTTAGATTTTTGGAATGCGCGTTCCTTGCTCCCTGAAACCGGTTTTCTAAAGCAGaa > 4:3406/1‑138 (MQ=255) cATGGGCATTTATTCAAAACGATATGAACGAATCCGAGATATATCGCGGGGAGCTGTCGTTTCTGTTCCGGATAAAGCGTTCGATTTTGGCAGGGCGCTTACGGTGCTCCAGGAAGCGGGTTTGCTAACGCTGAAAAAc < 1:131124/139‑1 (MQ=255) aTGGGCATTTATTCAAAACGATATGAACGAATCCGAGATATATCGCGGGGAGCTGTCGTGTGTGTTGCGGTTAAAGCGTTGGCTTTTGGCCGGGCGCTGTCGGTGGTCCAGGAAGCGGGGTTGGTAACGGGGCAAAACg > 8:33059/1‑139 (MQ=255) aTGGGCATTTATTCAAAACGATATGAACGAATCCGAGATATATCGCGGGGAGCTGTCGTGTGTGTTCCGGATAAAGCGGTGGCTTTTGGGAGGGCGCGTACGGTGGTCCCGGGAGCGGGTTTGCTAACGCTGTAAAACg > 7:67994/1‑139 (MQ=255) aTGGGCATTTATTCAAAACGATATGAACGAATCCGAGATATATCGCGGGGAGCTGTCGTGTGTGGTGCGGATAAAAAGTTGGATTTTGGCAGGGCCCGTTCGGTGCTACAGGAAACGGGGTTTGTAACGCTGaaaaaag > 2:264180/1‑137 (MQ=255) tGGGCATTTATTCAAAACGATATGAACGAATCCGAGATATATCGCGGGGAGCTGTCGTGTCTGGTGCGGAGAAACCCTTGGGTTTTGGCCGGGGGGGGAAGGGGCGGCCGGAGGCGGGTTTGGTTACGATGaaaaaag > 6:286836/1‑136 (MQ=255) tttATTCAAAACGATATGAACGAATCCGAGATATATCGCGGGGAGCTGTCGTTTCTGTTCCGGATAAAGCGTTCGATTTTGGCAGGGCGCTTACGGTGCTCCAGGAAGCGGGTTTGCTAACGCTGAAAAACGGATTTaa < 8:231292/139‑1 (MQ=255) tATTCAAAACGATATGAACGAATCCGAGATATATCGCGGGGAGCTGTCGTTTCTGTTCCGGATAAAGCGTTCGATTTTGGCAGGGCGCTTACGGTGCTCCAGGAAGCGGGTTTGCTAACGCTGAAAAACGGATTTAAc < 5:340343/138‑1 (MQ=255) aaaaCGATATGAACGAATCCGAGATATATCGCGGGGAGCTGTCGTTTCTGTTCCGGATAAAGCGTTCGATTTTGGCAGGGCGCTTACGGTGCTCCAGGAAGCGGGTTTGCTAACGCTGAAAAACGGATTTAACGGAAc < 4:384350/138‑1 (MQ=255) cGATATGAACGAATCCGAGATATATCGCGGGGAGCTGTCGTTTCTGTTCCGGATAAAGCGTTCGATTTTGGCAGGGCGCGTACGGTGCTCCAGGAAGCGGGTTTGCTAACGCTGAAAAACGGATTTAACGGAACAGGtt < 5:266743/139‑1 (MQ=255) tatGAACGAATCCGAGATATATCGCGGGGAGCTGTCGTGTGTGTTGCGGGTAAAGCGTTGGATTTTTGGAGGGGGCGTACGGGGGGCCCGGTAGCGGGGTTTGTAACGCGGAAAAACGGCTTTTACGGTACAgggtgag > 6:70017/1‑134 (MQ=255) aCGAATCCGAGATATATCGCGGGGAGCTGTCGTTTCTGTTCCGGATAAAGCGTTCGATTTTGGCAGGGCGCTTACGGTGCTCCAGGAAGCGGGTTTGCTAACGCTGAAAAACGGATTTAACGGAACAGGTTCAGTTGat < 1:264180/139‑1 (MQ=255) cGAATCCGAGATATATCGCGGGGAGCTGTCGTTTCTGTTCCGGATAAAGCGTTCGATTTTGGCAGGGCGCTTACGGTGCTCCAGGAAGCGGGTTTGCTAACGCTGAAAAACGGATTTAACGGAACAGGTTCAGTTGata < 2:252797/139‑1 (MQ=255) cGAGATATATCGCGGGGAGCTGTCGTTTCTGTTCCGGATAAAGCGTTCGATTTTGGCAGGGCGCTTACGGTGCTCCAGGAAGCGGGTTTGCTAACGCTGAAAAACGGATTTAACGGAACAGGTTCAATTGATATGAtt < 8:209387/138‑1 (MQ=255) tatCGCGGGGAGCTGTCGTTGGGGTTCCGGGTTAATAGGTAGATTTTTGGCCGGCGGTTAGCGTGGGCCAGGCGGCGGGGTTTGTAACGCTTAAAAAACGATTTAAAGGCACACGTTAAGTTTTTATGTTTTAAGATaa > 6:309220/1‑139 (MQ=255) gggAGCTGTCGTTTCTGTTCCGGATAAAGCGTTCGATTTTGGCAGGGCGCTTACGGTGCTCCAGGAAGCGGGTTTGCTAACGCTGAAAAACGGATTTAACGGAACAGGTTCAGTTGATATGATTAAAGATAATCCgaga < 5:309220/139‑1 (MQ=255) tCGTTTCTGTTCCGGATAAAGCGTTCGATTTTGGCAGGGCGCTTACGGTGCTCCAGGAAGCGGGTTTGCTAACGCTGAAAAACGGATTTAACGGAACAGGTTCAGTTGATATGATTAAAGATAATCCGAGACACCTg < 1:26548/137‑1 (MQ=255) tCGTTTCTGTTCCGGATAAAGCGTTCGATTTTGGCAGGGCGCTTACGGTGCTCCAGGAAGCGGGTTTGCTAACGCTGAAAAACGGATTTAACGGAACAGGTTCAGTTGATATGATTAAAGATAATCCGAGACACCTg < 3:7354/137‑1 (MQ=255) gATAAAGCGTTCGATTTTGGCAGGGCGCTTACGGTGCTCCAGGAAGCGGGTTTGCTaa > 2:39949/1‑58 (MQ=255) gATAAAGCGTTCGATTTTGGCAGGGCGCTTACGGTGCTCCAGGAAGCGGGTTTGCTaa < 1:39949/58‑1 (MQ=255) gATAAAGCGTTCGATTTTGGCAGGGCGCTTACGGTGCTCCAGGAAGCGGGTTTGCTAACGCTGAAAAACGGATTTAACGGAACAGGTTCAGTTGATATGATTAAAGATAATCCGAGACACCTGAAATTAAAAGCTGTcc > 3:35236/1‑139 (MQ=255) tAAAGCGTTCGATTTTGGCAGGGCGCTTACGGTGCTCCAGGAAGCGGGTTTGCTAACGCTGAAAAACGGATTTAACGGAACAGGTTCAGTTGATATGATTAAAGATAATCCGAGACACCTGAAATTAAAAGCTGTCCgg < 7:146996/139‑1 (MQ=255) aGCGTTCGATTTTGGCAGGGCGCTTACGGTGCTCCAGGAAGCGGGTTTGCTAACGCTGAAAAACGGATTTAACGGAACAGGTTCAGTTGATATGATTAAAGATAATCCGAGACACCTGAAATTAAAAGCTGTCCGGCaa > 1:113039/1‑139 (MQ=255) aGCGTTCGATTTTGGCAGGGCGCTTACGGTGCTCCAGGAAGCGGGTTTGCTAACGCTGAAAAACGGATTTAACGGAACAGGTTCAGTTGATATGATTAAAGATAATCCGAGACACCTGAAATTAAAAGCTGTCCGGCaa > 8:316734/1‑139 (MQ=255) cGATTTTGGCCGGGCGCGTACGGTGCTCCAGGAAGCGGGTTTGCTAACGCTGAAAAACGGATTTAACGGAACAGGTTCAGTTGATATGATTAAAGATAATCCGAGCCACCTGAAATTAAAAGCTGTCCGGCAACAGGAt < 7:360114/139‑1 (MQ=255) tttGGCATGGCGCTTACGGTGCTCCAGGAAGCGGGTTTGCTAACGCTGAAAAACGGATTTAACGGAACAGGTTCAGTTGATATGATTAAAGATAATCCGAGACACCTGAAATTAAAAGCTGTCCGGCAACAGGATGCa < 3:3406/138‑1 (MQ=255) tGGCAGGGCGCTTACGGTGCTCCAGGAAGCGGGTTTGCTAACGCTGAAAAACGGATTTAACGGAACAGGTTCAGTTGat > 8:50565/1‑79 (MQ=255) tGGCAGGGCGCTTACGGTGCTCCAGGAAGCGGGTTTGCTAACGCTGAAAAACGGATTTAACGGAACAGGTTCAGTTGat < 7:50565/79‑1 (MQ=255) gCAGGGCGCTTACGGTGCTCCATGAAGCGGGTTTGCTAACGCTGAAAAACGGATTTAACGGAACAGGTTCAGTTGATATGATTAAAGATAATCCGAGACACCTGAAATTAAAAGCTGTCCGGCAACAGGATGCAGTGAg < 7:71987/139‑1 (MQ=255) gCAGGGCGCTTACGGTGCTCCAGGAAGCGGGTTTGCTAACGCTGAAAAACGGATTTAACGGAACAGGTTCAGTTGATATGATTAAAGATAATCCGAGACACCTGAAATTAAAAGCTGTCCGGCAACAGGATGCAGTGAg < 8:79572/139‑1 (MQ=255) gCAGGGCGCTTACGGTGCTCCAGGAAGCGGGTTTGCTAACGCTGAAAAACGGATTTAACGGAACAGGTTCAGTTGATATGATTAAAGATAATCCGAGACACCTGAAATTAAAAGCTGTCCGGCAACAGGATGCAGTGAg < 5:70017/139‑1 (MQ=255) gCAGGGCGCTTACGGTGCGCCAGGAAGCGGGTTTGCTAACGCTGAAAAACGGATTTAACGGAACAGGTTCAGTTGATATGATTAAAGATAATCCGAGACACCTGAAATTAAAAGCTGTCCGGCAACAGGATGCAGTGAg < 5:122471/139‑1 (MQ=255) gggCGCTTACGGTGCTCCAGGAAGCGGGTTTGCTAAGGCTGAAAAACGGa > 6:283253/1‑50 (MQ=255) gggCGCTTACGGTGCTCCAGGAAGCGGGTTTGCTAACGCTGAAAAACGGa < 5:283253/50‑1 (MQ=255) | CTTAATTTGGCACCGCTCGGGACGACATACATAACGCCCATGGGCATTTATTCAAAACGATATGAACGAATCCGAGATATATCGCGGGGAGCTGTCGTTTCTGTTCCGGATAAAGCGTTCGATTTTGGCAGGGCGCTTACGGTGCTCCAGGAAGCGGGTTTGCTAACGCTGAAAAACGGATTTAACGGAACAGGTTCAGTTGATATGATTAAAGATAATCCGAGACACCTGAAATTAAAAGCTGTCCGGCAACAGGATGCAGTGAG > CP006881/948217‑948482 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |