BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	3,741	G→T	9.7%	intergenic (+26/‑130)	eryR → / → ampR	/vntifkey=4//vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	3,741	0	G	T	9.7%	14949.1 / inf	5150	intergenic (+26/‑130)	eryR/ampR	/vntifkey=4//vntifkey=4
Reads supporting (aligned to +/- strand): ref base G (158/4359); new base T (537/68); total (717/4433)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 5314 total aligned reads displayed.

ATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATT  >  Exported/3603‑3879

aTCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTTATAGTTATCTATTATTTACCGGGCGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTg                                                                                                                                            <  5:182333/139‑1 (MQ=255)

aTCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTg                                                                                                                                            <  4:95865/139‑1 (MQ=255)

aTCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTg                                                                                                                                            >  8:377615/1‑139 (MQ=255)

  cAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGa                                                                                                                                          <  4:396604/139‑1 (MQ=255)

   aGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGa                                                                                                                                          <  7:129747/138‑1 (MQ=255)

     tttCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTTGAAAg                                                                                                                                       >  1:221763/1‑139 (MQ=255)

        catcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGtt                                                                                                                                     <  8:198020/138‑1 (MQ=255)

         atcaAGCAATGAAACACTCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGATGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTa                                                                                                                                    <  8:134268/138‑1 (MQ=255)

         atcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTa                                                                                                                                    <  8:10814/138‑1 (MQ=255)

         atcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTa                                                                                                                                    <  1:58036/138‑1 (MQ=255)

         atcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTAACATTACTTATGAGCAAGTATTGTCTATTTTTAATAGATATCTATTATTTAACGGGAGGAATTAATTCTATGAGTCTC‑GGTTTTTAATTTTGTAAGtat                                                                                                                                    >  1:100030/1‑136 (MQ=255)

          tcaAGCAATGAAACACGCCAAAGTAAACAATTTAGGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                  <  6:10958/139‑1 (MQ=255)

          tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                  <  4:59830/139‑1 (MQ=255)

          tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                  <  3:79729/139‑1 (MQ=255)

          tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                  <  5:122577/139‑1 (MQ=255)

          tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                  <  5:338137/139‑1 (MQ=255)

          tcaAGAAATAAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                  <  2:190555/139‑1 (MQ=255)

            aaGCAATGAAACACGCCTAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                                <  7:320269/139‑1 (MQ=255)

            aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                                <  6:137561/139‑1 (MQ=255)

            aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                                <  5:86293/139‑1 (MQ=255)

            aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                                <  8:314806/139‑1 (MQ=255)

            aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                                <  7:85623/139‑1 (MQ=255)

            aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                                <  5:361384/139‑1 (MQ=255)

            aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                                <  7:208314/139‑1 (MQ=255)

                    aaaCACGCCAAAGGAAACAATATAACGACCATTACTTAAGAGCAACTATTCTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTaaa                                                                                                                        <  2:358550/139‑1 (MQ=255)

                           ccAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATg                                                                                                                 <  3:202008/139‑1 (MQ=255)

                                gTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATATATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAt                                                                                                            <  2:98095/139‑1 (MQ=255)

                                 tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                           <  2:62418/139‑1 (MQ=255)

                                  aaaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                           <  2:285931/138‑1 (MQ=255)

                                  aaaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                           <  2:156092/138‑1 (MQ=255)

                                   aaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATaaa                                                                                                         <  7:372662/139‑1 (MQ=255)

                                     cAATTTAAGTACCATTACTTATGAGCAAGAATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAatt                                                                                                       <  4:262009/139‑1 (MQ=255)

                                        tttAAGTACCATTACTTATGATCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGATAGTTACTCGTTACTAAAGGGAATGGAGATAAattatt                                                                                                    <  1:250589/139‑1 (MQ=255)

                                        tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                    <  2:404789/139‑1 (MQ=255)

                                        tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                    <  2:318644/139‑1 (MQ=255)

                                        tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                    <  1:370082/139‑1 (MQ=255)

                                        tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGTTATTTTAACGTTTCTAAAGGGAATGTATATaaaatttt                                                                                                    >  8:168389/1‑134 (MQ=255)

                                            aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                                <  3:300826/139‑1 (MQ=255)

                                            aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                                <  2:118536/139‑1 (MQ=255)

                                            aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                                <  6:45567/139‑1 (MQ=255)

                                            aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                                <  4:365810/139‑1 (MQ=255)

                                             gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATATATTATTTAACGGGTGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGTATGGAGATAAATTATTAGat                                                                                                <  8:231030/138‑1 (MQ=255)

                                                ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTTGTAATTTTAAACTTACTAAATGGAATTGAGATAAATAATTATTtataa                                                                                            >  5:204526/1‑138 (MQ=255)

                                                         aTGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                                     <  8:264632/137‑1 (MQ=255)

                                                          tGATCAAGTATTGTCTATATTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAg                                                                                   <  1:299732/138‑1 (MQ=255)

                                                           gAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTTATTTTGAAAAATTCACCGTTCTAAATGGAATTGTGATAAATTATTTTATATACTAATGACATCt                                                                                 >  3:403224/1‑139 (MQ=255)

                                                           gAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTTGAAAATTACACATTAAAAAACGGAAATGAGAATAAATATTATATATAATAATGACAgaa                                                                                 >  8:409600/1‑137 (MQ=255)

                                                               aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                             <  4:104201/139‑1 (MQ=255)

                                                               aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                             <  5:255632/139‑1 (MQ=255)

                                                                aGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAAATTT‑GTAATTTACACGTTACAAAAGCTAAGGGAGTTAAATTATTATCAATAATACTCACAgaatgca                                                                             >  8:34509/1‑132 (MQ=255)

                                                                aGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTTTTAAGTTTAACGGTTATAAATGGAATGGTGATAAATTATTTTATATACTAATGAAAGCTTCCa                                                                             >  3:177987/1‑138 (MQ=255)

                                                                aGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GGTTTTTAATTTTGAAAGTTTAACGTTAATAAATGGAATGGAGATAAATTATTTTATATAATAATTACAGCATCCa                                                                             >  1:271948/1‑138 (MQ=255)

                                                                aGTATTGTATATTTTTAATAGTTATCTATTATTTAACGGGATGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTTCTGACAGCTTCCaa                                                                            <  6:202280/139‑1 (MQ=255)

                                                                 gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                           <  4:22979/139‑1 (MQ=255)

                                                                 gTATTGTCTATTTTTAATAGTTATCTATTATTTAAAGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                           <  6:338647/139‑1 (MQ=255)

                                                                  tATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                           <  6:27789/138‑1 (MQ=255)

                                                                   aTTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                          <  3:54821/138‑1 (MQ=255)

                                                                    ttGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaagaag                                                                        <  1:191978/139‑1 (MQ=255)

                                                                         tATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTaaa                                                                   <  7:184520/139‑1 (MQ=255)

                                                                         tATTTTTAATAGTTATCTATTATTAAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCaaaa                                                                   <  5:279503/139‑1 (MQ=255)

                                                                           tttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTaaa                                                                   <  3:263848/137‑1 (MQ=255)

                                                                             tttAATAGTTATCTATTATTTAACTGGAGTAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGTAATGGAGATAAATTATTTGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                <  4:169772/138‑1 (MQ=255)

                                                                             tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                <  3:324774/138‑1 (MQ=255)

                                                                             tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                <  5:390695/138‑1 (MQ=255)

                                                                             tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                <  2:228755/138‑1 (MQ=255)

                                                                              ttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAgg                                                               <  6:175431/138‑1 (MQ=255)

                                                                                  tAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCt                                                          <  3:122397/139‑1 (MQ=255)

                                                                                    gTTATGTATTATTTAACGGGAGGAAATAATTCTATGAGGCGC‑TTTTTTAAATTTGCAAAGTTACACGTTACTAATGGGAATGGTCATAAATTATTAGATATACTACTGACAGCTTCCCAGAAGCTAAAGAGGGCCCTAg                                                        <  8:72856/139‑1 (MQ=255)

                                                                                       aTCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGTAATTTTAACCTTAATAAATGGAATGGATATAAATTATTTTATATAATACTTACAGCGTCCAAGAAGCTAAATAGGTCCCGAgcgc                                                     >  4:329606/1‑139 (MQ=255)

                                                                                          tattatTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTa                                                  <  5:227817/139‑1 (MQ=255)

                                                                                            ttattTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCt                                                   <  3:347792/136‑1 (MQ=255)

                                                                                              attTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACgg                                               <  6:308604/138‑1 (MQ=255)

                                                                                                   aCGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAAtt                                         <  7:251788/139‑1 (MQ=255)

                                                                                                       gaggaAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgg                                     <  7:105868‑M1/139‑3 (MQ=255)

                                                                                                          gaAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggta                                  <  6:78009‑M1/139‑6 (MQ=255)

                                                                                                            aataatTCTATGAGTGGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                <  6:240523‑M1/139‑8 (MQ=255)

                                                                                                            aataatTCTATGAGGCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                <  2:25732‑M1/139‑8 (MQ=255)

                                                                                                                   cTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaa                          <  3:372296‑M1/138‑14 (MQ=255)

                                                                                                                      tgcttcgc‑tTTTTTAAATTTTGAAAGTTACACGTTACTAAAGGGAATGGATATAAATTATTATATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                      <  1:162695‑M1/135‑18 (MQ=255)

                                                                                                                      tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                      <  8:289207‑M1/139‑18 (MQ=255)

                                                                                                                      tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                      <  5:156626‑M1/139‑18 (MQ=255)

                                                                                                                      tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                      <  1:127438‑M1/139‑18 (MQ=255)

                                                                                                                      tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                      <  7:23000‑M1/139‑18 (MQ=255)

                                                                                                                      tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                      <  4:299161‑M1/139‑18 (MQ=255)

                                                                                                                      tGAGTAGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                      <  1:84309‑M1/139‑18 (MQ=255)

                                                                                                                       tagTCTC‑TTTTTTAAATTTGGAAAGTTTCACGTTACTAAAGGGAATGTAGATAAATTATTAGATATACTACTGACAGCTTCAAAGAATCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                      <  7:398766‑M1/137‑18 (MQ=255)

                                                                                                                       gAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                      <  3:147878‑M1/138‑18 (MQ=255)

                                                                                                                       gAGTCGC‑TTTTTTAAATTTGGAAAGTTAAACGTTAATAAAGGGAATGGAGTTAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                      <  7:158331‑M1/138‑18 (MQ=255)

                                                                                                                        aGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                    <  6:379372‑M1/139‑20 (MQ=255)

                                                                                                                        aGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                    <  7:297633‑M1/139‑20 (MQ=255)

                                                                                                                        aGTCGC‑TTTTTTAAATTTGGAAAGTTAAACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagga                     <  1:337772‑M1/138‑19 (MQ=255)

                                                                                                                            gC‑TTTTTTAAATTTGGAAAGTTACACTTTACTAAAGGTAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagag                 <  5:305741‑M1/138‑23 (MQ=255)

                                                                                                                               ttttttAATTTTGCAAATTTCCACGTTACTAAAGGTAATGGAGATATATTATTAGATATACTAATTACAGCTGCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat              <  3:235312‑M1/139‑26 (MQ=255)

                                                                                                                               ttttttAAATTTGGAAATTTACAAGTTAATAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat              <  7:345753‑M1/139‑26 (MQ=255)

                                                                                                                                 ttttAAATTTGTAAAGTTACACGTTACTAAAGTGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat              <  7:273621‑M1/137‑26 (MQ=255)

                                                                                                                                 ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCCAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat              <  1:399264‑M1/137‑26 (MQ=255)

                                                                                                                                 ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat              <  4:137036‑M1/137‑26 (MQ=255)

                                                                                                                                     aaaTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta         <  7:58916‑M1/138‑31 (MQ=255)

                                                                                                                                      aaTTTGTCAATTTACACGTTAATAAAGGGAATGGACATAAATTATTATTTATAATAATGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGTGAATTTGgggtacattgaaaaaggaagagtatgagtatt       <  5:58239‑M1/139‑33 (MQ=255)

                                                                                                                                      aaTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtatt       <  4:198681‑M1/139‑33 (MQ=255)

ATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATT  >  Exported/3603‑3879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`