Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 3,783 G→T 5.4% intergenic (+68/‑88) eryR → / → ampR /vntifkey=4//vntifkey=4

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported3,7830GT 5.4% 16237.0 / 115.4 5022intergenic (+68/‑88)eryR/ampR/vntifkey=4//vntifkey=4
Reads supporting (aligned to +/- strand):  ref base G (1484/3095);  new base T (342/70);  total (1845/3177)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 5168 total aligned reads displayed.

TAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTG  >  Exported/3645‑3923
                                                                                                                                           |                                                                                                                                            
 aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                                                              <  6:79994/138‑1 (MQ=255)
 aaGTACCATTACTTAAGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                                                              <  4:303567/138‑1 (MQ=255)
             ttATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                                                                                 <  1:402993/139‑1 (MQ=255)
                tGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGc                                                                                                                              <  5:386334/139‑1 (MQ=255)
                 gAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTTGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTATATATAATACTGACAGCt                                                                                                                             >  7:293562/1‑139 (MQ=255)
                 gAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGAAAGTTTCACGGTTCATAAGGGTATTGTGATTAATTATTTGTTATAATACTTACAGCt                                                                                                                             >  2:242697/1‑139 (MQ=255)
                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                                                         <  5:245534/139‑1 (MQ=255)
                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                                                         <  1:373768/139‑1 (MQ=255)
                     aaGTATTGTATATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                                                         <  5:302151/139‑1 (MQ=255)
                      aGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAATTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaa                                                                                                                        <  1:30784/139‑1 (MQ=255)
                       gTATTGTCTATTTTTTATAGTTATCTATTATATAACGGGTCGATATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTTCACGTTACTTAAGTGAATGGAGTTAAATTATTAGTTATACTACTGACAGCTTcgatg                                                                                                                       <  4:74623/139‑5 (MQ=255)
                       gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGTGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                                                                       <  1:62226/139‑1 (MQ=255)
                       gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTTATTTTTTAATTTTCAAGTTTATAAATGGTATTGAGATTAATTATTTTATTTAATAATGACCGCGTcc                                                                                                                          >  3:345846/1‑136 (MQ=255)
                         aTTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                                                                      <  3:54821/138‑1 (MQ=255)
                            gTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTTGTAAATTACACGGTAATTAAAGGAATTGTGATAAATTATTATATATAATAAATACAACTTACAACAAGc                                                                                                                   >  8:26161/1‑138 (MQ=255)
                             tCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGATAATTAAACCTTAGTAAAGGGAATTGATATAAAATTTTATATTTTATAATTACAGCTTGCAAGAAGCt                                                                                                                  >  5:166718/1‑138 (MQ=255)
                                   tttACTAGATATCTATTATTTAACGGGAGGAAATTATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAACGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                                                            <  3:84760/138‑1 (MQ=255)
                                   tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                                                            <  1:216038/138‑1 (MQ=255)
                                   tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                                                            <  5:75594/138‑1 (MQ=255)
                                   tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                                                            <  7:186342/138‑1 (MQ=255)
                                       aTAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTAAAATTTTTAATTTAGACCATACTAAAGGGAATGGTGATAAATTATTATATAAAATAGTCACCCCATGCAAGCAGCTAAACAGGTgcc                                                                                                       >  3:155348/1‑139 (MQ=255)
                                          gTTATGTATTATTTAACGGGAGGAAATAATTCTATGAGGCGC‑TTTTTTAAATTTGCAAAGTTACACGTTACTAATGGGAATGGTCATAAATTATTAGATATACTACTGACAGCTTCCCAGAAGCTAAAGAGGGCCCTAg                                                                                                    <  8:72856/139‑1 (MQ=255)
                                          gTTATCTATTATTTACCGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGCAAAGTAACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAg                                                                                                    <  6:122928/139‑1 (MQ=255)
                                          gTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAg                                                                                                    <  3:209000/139‑1 (MQ=255)
                                             aTCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTTGTAATTTTAACGTTTATAAAGGGGATGTTGATAAATTATTTTATATACTAATGACAGCTTCCAATAAGCAAAAGAGGTCCCTAgcgc                                                                                                 >  3:181254/1‑139 (MQ=255)
                                                  ttattTAACGTGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCt                                                                                               <  8:236864/136‑1 (MQ=255)
                                                    attTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACgg                                                                                           <  7:242667/138‑1 (MQ=255)
                                                         aCGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAAt                                                                                      <  6:29464/138‑1 (MQ=255)
                                                          cGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGTAATTTTAACGGTTCAAAATGGTATGGAGATAAAATATTTTATTTAATTAAGAAACCTTACAAGAAGCTAAAGAGTTGACAAGCGCAGACCGGGAAttt                                                                                    >  8:318923/1‑139 (MQ=255)
                                                            ggaggaAATAATTCTATGAGTCGC‑GTTTTTAAATTTTGATATTTTCACGTTTGTAAAGGGAATGGAGATAAATTTTTTGATTTAATTCTGCCAGCGTACAAGCAGCATAAGAGGTGACTAGCGCCGACGGGGAAttttg                                                                                  >  8:206092/1‑137 (MQ=255)
                                                             gaggaAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGTAAGTTACACGTTAATAAAGGGTATGGGGATAAATTATTTTATATAATACTGACAGCTTCCAATAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgg                                                                                 >  1:102692‑M1/1‑137 (MQ=255)
                                                              agtaaataaTTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTATATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGggg                                                                                <  5:220621‑M1/136‑4 (MQ=255)
                                                              aggaAAAAATTCTCTGAGTCGC‑TTTTTTAAATTTGCAAAGTTACACGTTACTAAAGGGAAGGGAGATAAATTATTAGATATACTACTGACAGCTGCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGggg                                                                                <  2:65272‑M1/139‑4 (MQ=255)
                                                               ggaAATAATTCTATGAGTCGCGTTTTTTAATTTTGTAAATTTA‑ACGGTAATAAAAGGAATGGAGATAAATTTTTTTATATAATTATGACAGCATACAAGAAGCTAAAGAGGTGCCTAGCGCCAACGGGGAATTTGtggg                                                                               >  2:14612/1‑136 (MQ=255)
                                                                  aataatTCTATGAGTTGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGTAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                                                            <  8:350859‑M1/139‑8 (MQ=255)
                                                                  aataatTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                                                            <  5:275139‑M1/139‑8 (MQ=255)
                                                                         cTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTATATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaa                                                                      <  1:251359‑M1/138‑14 (MQ=255)
                                                                         cTATGAGTCGA‑TTTTTTAAATTTGGAAACTTACACGTTACTAAAGGGAATGCAGATAAATTATTACCTATACTACAGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaa                                                                      <  6:361432‑M1/138‑14 (MQ=255)
                                                                         ataTGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAAGTTAATAAAGTGAATGGAGATAAATTATTATATATAATACTGACATATTCAAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaa                                                                      <  1:279994‑M1/137‑14 (MQ=255)
                                                                            tgcttcgc‑tTTTTTAAATTTTGAAAGTTACACGTTACTAAAGGGAATGGATATAAATTATTATATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                  <  1:162695‑M1/135‑18 (MQ=255)
                                                                            tGAGTCGC‑TTTTTTAAATTTGTAAAGTTAAACGTTACAAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                  <  2:373380‑M1/139‑18 (MQ=255)
                                                                            tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                  <  6:323009‑M1/139‑18 (MQ=255)
                                                                            tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                  <  5:145527‑M1/139‑18 (MQ=255)
                                                                             gtgtcGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGTATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                  <  3:320183‑M1/136‑18 (MQ=255)
                                                                             gAGTCGC‑TTTTTTAATTTTGGAAATTTACACGTTACTAAAGGGAATGGAGATAATTTATTAGATATACTACTGACTGCTTCCATGTAGCTATTGAGGGCCCTAGCGCCTACGGGGATTTT‑‑‑‑‑GGGGTACATTGAAAAAgg                                                             <  2:330040/138‑1 (MQ=255)
                                                                             gAGTCGC‑TTTTTTAATTTGTGAAATTTACCAGTTACTAAAGGGAATGGATATAAATTATTAGATATACTACTGCCAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                  <  4:240475‑M1/138‑18 (MQ=255)
                                                                             gAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                  <  6:215513‑M1/138‑18 (MQ=255)
                                                                             gAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                  <  5:9941‑M1/138‑18 (MQ=255)
                                                                             gAGTCGA‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                  <  1:125622‑M1/138‑18 (MQ=255)
                                                                              agtatc‑tttttTTAATTTGGAAAGTTACACGTTACTAAAGTGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                                                                <  1:82557‑M1/134‑20 (MQ=255)
                                                                              aGTCGC‑TTTTTTAAATTTGGAAAGTTACACTTTACTAAAGGGAATGGAGATAAATTATTATATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                                                                <  2:113339‑M1/139‑20 (MQ=255)
                                                                              aGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                                                                <  7:105921‑M1/139‑20 (MQ=255)
                                                                              aGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                                                                <  3:263680‑M1/139‑20 (MQ=255)
                                                                              aGTCGA‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                                                                <  2:272509‑M1/139‑20 (MQ=255)
                                                                               gTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                                                                <  2:409479‑M1/138‑20 (MQ=255)
                                                                               gTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                                                                <  3:401536‑M1/138‑20 (MQ=255)
                                                                                  gC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagag                                                             <  5:357131‑M1/138‑23 (MQ=255)
                                                                                       ttttAAATTTGGAAAGTTACACGTTACTAAAGGTAATTGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGACGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                          <  1:340298‑M1/137‑26 (MQ=255)
                                                                                       ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                          <  7:26952‑M1/137‑26 (MQ=255)
                                                                                       ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                          <  7:217296‑M1/137‑26 (MQ=255)
                                                                                            aaTTTGGAAAGTTACACGTTACTAAAGGGAATGTAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtatt                                                   <  8:379038‑M1/139‑33 (MQ=255)
                                                                                                 ggAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaaca                                              >  5:196684‑M1/1‑102 (MQ=255)
                                                                                                    aatttacaCGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTGCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatt                                            <  8:175423‑M1/135‑40 (MQ=255)
                                                                                                           acGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtc                                    <  7:59341‑M1/139‑48 (MQ=255)
                                                                                                               tACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccc                                >  4:411721‑M1/1‑88 (MQ=255)
                                                                                                                 cTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccct                               >  4:267751‑M1/1‑86 (MQ=255)
                                                                                                                 cTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccct                               >  8:289517‑M1/1‑86 (MQ=255)
                                                                                                                 cTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccag                               >  3:37114‑M1/1‑86 (MQ=255)
                                                                                                                 cTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGACTACGGGGAATTTGggttacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccct                               >  2:211683‑M1/1‑86 (MQ=255)
                                                                                                                    cagGGAATGCAGATACATTATTAGCTATACTCCTGCCAGCTGCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGggggacattgaaaaaggcagagtatgagtattcaacatttccgtgtcgcccttatt                           <  7:373080‑M1/138‑57 (MQ=255)
                                                                                                                       ggAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccc                        >  3:235937‑M1/1‑80 (MQ=255)
                                                                                                                       ggAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgt                                     <  5:113586‑M1/126‑47 (MQ=255)
                                                                                                                         aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGATTTTGgggtacattgaaaaaggaagagtatgagtattcaccatttccgtgtcgcccttattccctt                      >  6:51457‑M1/1‑78 (MQ=255)
                                                                                                                         aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                      >  7:349807‑M1/1‑78 (MQ=255)
                                                                                                                         aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                      >  1:185887‑M1/1‑78 (MQ=255)
                                                                                                                         aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                      >  4:112310‑M1/1‑78 (MQ=255)
                                                                                                                         aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                      >  5:324240‑M1/1‑78 (MQ=255)
                                                                                                                         aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                      >  7:860‑M1/1‑78 (MQ=255)
                                                                                                                         aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                      >  4:371611‑M1/1‑78 (MQ=255)
                                                                                                                         aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccct                      >  3:128397‑M1/1‑78 (MQ=255)
                                                                                                                         aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcg                                   <  3:29490‑M1/126‑49 (MQ=255)
                                                                                                                         aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                  <  5:48516‑M1/95‑18 (MQ=255)
                                                                                                                         aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                  >  6:256426‑M1/1‑78 (MQ=255)
                                                                                                                         aaTGGAGATAAATTATTAGAAATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                  <  4:17713‑M1/95‑18 (MQ=255)
                                                                                                                           tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACgg                                                                                           >  6:394868/1‑68 (MQ=255)
                                                                                                                           tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctttt                    >  8:141851‑M1/1‑76 (MQ=255)
                                                                                                                           tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctttt                    >  3:102404‑M1/1‑76 (MQ=255)
                                                                                                                           tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccgtattccctttt                    >  7:322374‑M1/1‑76 (MQ=255)
                                                                                                                           tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                  >  4:334641‑M1/1‑76 (MQ=255)
                                                                                                                           tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                  <  8:409176‑M1/93‑18 (MQ=255)
                                                                                                                                aTAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                  <  2:304577‑M1/88‑18 (MQ=255)
                                                                                                                                 tAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcg               <  6:150977‑M1/138‑69 (MQ=255)
                                                                                                                                  aaaTTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttatgcgg              >  7:398747‑M1/1‑69 (MQ=255)
                                                                                                                                   aaTTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccctt                              <  7:163729‑M1/121‑54 (MQ=255)
                                                                                                                                    attattAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcat           >  4:20351‑M1/1‑67 (MQ=255)
                                                                                                                                        ttaGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttg       >  8:112221‑M1/1‑63 (MQ=255)
                                                                                                                                        ttaGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgt                                     <  4:141985‑M1/109‑47 (MQ=255)
                                                                                                                                        ttaGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                  <  6:186448‑M1/80‑18 (MQ=255)
                                                                                                                                          aGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgcc     >  7:133257‑M1/1‑61 (MQ=255)
                                                                                                                                          aGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattaccttttttgcggcattttgcc     >  2:180399‑M1/1‑61 (MQ=255)
                                                                                                                                           |                                                                                                                                            
TAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTG  >  Exported/3645‑3923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑