BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	4,649	A→C	7.4%	Y260S (TAC→TCC) ‡	ampR →	/vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	4,649	0	A	C	7.4%	9929.5 / 180.8	3893	Y260S (TAC→TCC) ‡	ampR	/vntifkey=4
Reads supporting (aligned to +/- strand): ref base A (2532/993); new base C (14/350); total (2550/1343)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 4009 total aligned reads displayed.

TTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTT  >  Exported/4511‑4786

   cAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGAACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTacac                                                                                                                                        >  1:378960/1‑139 (MQ=255)

          ccTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGGTGATAAATCTGGAGCAGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTAAAACACgggg                                                                                                                                 >  5:237159/2‑139 (MQ=255)

          aCTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACCCGACgggg                                                                                                                                 >  4:188492/1‑139 (MQ=255)

          aCTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACgggg                                                                                                                                 >  8:14578/1‑139 (MQ=255)

                gcgcTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAg                                                                                                                           >  3:166733/1‑139 (MQ=255)

                 cgcTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAgg                                                                                                                          >  7:337056/1‑139 (MQ=255)

                 cgcTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGACCCGGTGAGATTGGGTCTCGCGGTATCATTTAAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGACAGTCAgc                                                                                                                          >  6:368272/1‑138 (MQ=255)

                   cTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTcctgtc                                                                                                                        >  1:277675/1‑134 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGGTGATAAATCTGGAGCCGGTCAGCGTGGGTCTCGCGGTATCATTCCAGCACTGGGGCCAGATGGTATGCCCTCCCGGAGCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAAc                                                                                                                      >  7:148718/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAAc                                                                                                                      >  5:107577/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAAc                                                                                                                      >  4:394701/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAAc                                                                                                                      >  2:72854/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAAc                                                                                                                      >  7:319894/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAAc                                                                                                                      >  1:317917/1‑138 (MQ=255)

                              cGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGa                                                                                                             <  5:140363/139‑1 (MQ=255)

                                    gctggTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGaaa                                                                                                       >  6:135604/1‑139 (MQ=255)

                                            tttGGTGATAAAGGTGGCGCGGGTGCGGGGGGGGCGCGCGGTTTCATTGCCCCAGGGGGGCCCGTGGTTACGCCCTCCCGTATCGTTTTTA‑TCTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAg                                                                                                <  8:240084/137‑1 (MQ=255)

                                               gtttaTAAAGCTGGCGCGGGGGAGGGGGGGTCGGGGGGTATCTTTGCCGCTCGGGGGCCCGATGGTAACCCCCCCCGTTTCGTTGATA‑TCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATc                                                                                             <  8:382227/134‑1 (MQ=255)

                                                cttaTAAATCGGGCGCGGGTGAGCGTGGGTCTCGCGTTATCATGGCACCACGGGGCCCATATGGAAAGCCCCCCCGTATCGTATTAA‑TCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCg                                                                                            <  2:307158/135‑1 (MQ=255)

                                                cggaaaaaTCTGGCGCCGGTGAGCGGGGGGCTCGGGGTATCATTGCCGCACTGGGGCCCGATGTTAAGCCCGCCCGTATCGTATTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGc                                                                                           <  7:2501/134‑1 (MQ=255)

                                                  gATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAAAGAAATAGACAGATCGCt                                                                                          >  1:100571/1‑138 (MQ=255)

                                                  gATAAATCTCTATCCGGTGAGCGTGCGTCTCGCGCTATCATTGCAGCACTGGGGCCAGATGATAAGCCCTCACGTATCGTAGTTA‑TCTACACGACTTGGAGACATTCAACTATCGATGAACGAAATAGACAGATCgat                                                                                          >  8:177752/1‑136 (MQ=255)

                                                   aTAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                                          >  6:191177/1‑137 (MQ=255)

                                                      taTCTGGCGCCGGGGAGCGGGGGACTCCCTGGATCATGCCAGCACTGGGCCCTGATGGTAAGCCCCCCCGTATCGTAGTTA‑TATACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGAt                                                                                     <  7:398635/138‑1 (MQ=255)

                                                             aGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAggctgt                                                                              >  7:95494/1‑135 (MQ=255)

                                                                cGGTGGGCGGGGGTCGCGGGGAATACTCGCCGCACGGGGGCCAGATGGTAACCCCCCCCGTCTGTTAGTTA‑ACACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                                           <  4:50611/139‑1 (MQ=255)

                                                                cGGTGCGGGTGGGTCGCGCGTTATCATTGCAGCACTGGGGCCATATGGTAAGCCCTCCCGTATCGTATTTA‑TCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                                           <  3:190069/139‑1 (MQ=255)

                                                                cGGTGCGGGTGGGGCGCGGGGTATCATTCCCGCTGGGGGGCCAGATGTTACGCCCGCCCTTTTCTTATTTA‑TCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                                           <  7:371872/139‑1 (MQ=255)

                                                                cGGTGAGGGGGGGGCTGGGGTTATCATTGCAGCAGGGGGGCCAGTTGGTAACCCCCCCCTTTTCGTATTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                                           <  7:175051/139‑1 (MQ=255)

                                                                cGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                                           <  5:206357/139‑1 (MQ=255)

                                                                  gTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGCCGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACt                                                                         >  6:337198/1‑139 (MQ=255)

                                                                      gCGGGGCTCTCGGGGTAAAATTGAAGCACTGGGGCCAGAGGGTACCCCCTCCCGTACCGTAGTAA‑TCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGAtt                                                                     <  8:323951/139‑1 (MQ=255)

                                                                       cGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGAtt                                                                     >  7:122273/1‑138 (MQ=255)

                                                                            gTCTCGCGGAATCATGGCCGCACGGGGGCCAGATGGAAAGCCCTCCCGTAGCGTAGTAA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCAt                                                               <  2:216667/139‑1 (MQ=255)

                                                                               tcGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTgg                                                            >  4:260171/1‑139 (MQ=255)

                                                                               tcGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTgg                                                            >  5:285040/1‑139 (MQ=255)

                                                                                cggggTATCATGGCAGCACTGGGGCCAGATGGTAACCCCGCCCGTATCTTCGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGt                                                           <  1:249977/136‑1 (MQ=255)

                                                                                cgcgGTATCATTGCAGCAGGGGGGCCAGTTGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGt                                                           <  1:34481/139‑1 (MQ=255)

                                                                                 gcgGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGCTTGGTGCCTCACTGATTAAGCATTGGt                                                           >  6:270829/1‑138 (MQ=255)

                                                                                       tCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGt                                                     >  3:67460/1‑138 (MQ=255)

                                                                                           tGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATTAACGAAATAGACAGATCTCTGAGATAGGTTCCTCACTGATTAAGCATTGGTAACTGTCata                                                 >  8:296901/1‑136 (MQ=255)

                                                                                           tGCAGCACAGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACCGGGAGTCAGGCAACTAAGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTTATTAAGCATTGGTAAATGACata                                                 >  1:158448/1‑136 (MQ=255)

                                                                                               gcaCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGCCAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAg                                            >  8:213671/1‑139 (MQ=255)

                                                                                               gcaCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                                          >  8:346097/1‑93 (MQ=255)

                                                                                               gcaCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAg                                            >  3:224304/1‑139 (MQ=255)

                                                                                               gcaCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAg                                            >  1:18980/1‑139 (MQ=255)

                                                                                               gcaCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAg                                            <  2:44042/139‑1 (MQ=255)

                                                                                               gcaCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAg                                            >  2:245568/1‑139 (MQ=255)

                                                                                                 cggggggCCAGATGGTAACCCCTCCCGTATCGTTGTTA‑TCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                                          <  4:222490/136‑1 (MQ=255)

                                                                                                 aCTGGGGCCAGTTGTTAAGCCCTCCCGTATCTTAGTTA‑TATACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                                          <  4:315917/139‑1 (MQ=255)

                                                                                                  cTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                                          >  2:37188/1‑138 (MQ=255)

                                                                                                  cTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                                          >  7:51978/1‑138 (MQ=255)

                                                                                                   tGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAAGTGTCAGACCAAGTTTa                                        >  4:293282/1‑139 (MQ=255)

                                                                                                   tGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        >  6:169815/1‑139 (MQ=255)

                                                                                                   tGGGGCCAGATGGAACGCCCTCCCGTATCGTAGTTA‑ACTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        <  5:72281/139‑1 (MQ=255)

                                                                                                   tGGGGCCAGAGTGTAAGCCATCCCGTTTCGTTTTTA‑TCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        <  4:340918/139‑1 (MQ=255)

                                                                                                   gggggCCCGATGGAACCCCCCCCCGAAACGTAGTTA‑TCTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        <  5:179409/138‑1 (MQ=255)

                                                                                                   gggggCCAGTTGGTAACCCCTCCCGTTTCTTTGTTA‑TATACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        <  6:154551/138‑1 (MQ=255)

                                                                                                   gggggCCAGATGGTACGCCCCCCCTAAGCTTATTTA‑ACAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        <  5:406586/138‑1 (MQ=255)

                                                                                                   ggggcCCTGATGTTACGCCCTCCGGTATCTTATTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        <  6:100756/138‑1 (MQ=255)

                                                                                                   ggggcCCAGATGGTACCCCCCCCCGTATGGTAGTTA‑CCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        <  3:380151/138‑1 (MQ=255)

                                                                                                   ggggcCCAGATGGAACCCCCTCCCGTATGGTATTTA‑TCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        <  1:52492/138‑1 (MQ=255)

                                                                                                     gtcccAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACAAAACAGTAATTCAGTCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGACTCACTGATTAAGCATTGGTAACTGACAGACCAAGTTAAca                                      >  6:6570/4‑138 (MQ=255)

                                                                                                     gggCCATTTGTTAACCCCTCCCGTCTCGTAGTTA‑TTTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        <  8:69563/137‑1 (MQ=255)

                                                                                                     gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAc                                                <  7:294932/129‑1 (MQ=255)

                                                                                                     gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt                                      >  3:348615/1‑139 (MQ=255)

                                                                                                     gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt                                      >  2:112136/1‑139 (MQ=255)

                                                                                                     gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt                                      >  3:138352/1‑139 (MQ=255)

                                                                                                     gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACGGTCAGACCAAGTTTACt                                      >  6:91245/1‑139 (MQ=255)

                                                                                                     gggCCAGAGGGAATGCCCACCCGAATCG‑ATTTATTCTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        <  8:52837/137‑1 (MQ=255)

                                                                                                     ggcccAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        <  7:2719/134‑1 (MQ=255)

                                                                                                           gATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatata                                <  3:297219/139‑1 (MQ=255)

                                                                                                            aTGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatata                                >  2:346648/1‑138 (MQ=255)

                                                                                                               gTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGCCCAAGTTTACTCATATATACt                            >  5:326415/1‑139 (MQ=255)

                                                                                                               gTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACt                            >  4:370351/1‑139 (MQ=255)

                                                                                                                tAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                                           >  7:70111/1‑91 (MQ=255)

                                                                                                                tAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACtt                           >  3:322284/1‑139 (MQ=255)

                                                                                                                 aaGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACtt                           >  2:180430/1‑138 (MQ=255)

                                                                                                                 aaGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATAGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACtt                           >  1:131902/1‑138 (MQ=255)

                                                                                                                     ccTCCCGTATCTTATTTA‑TATACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATAGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgat                      <  5:356078/139‑1 (MQ=255)

                                                                                                                     ccTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgat                      <  2:139697/139‑1 (MQ=255)

                                                                                                                      cTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgatt                     >  4:81137/1‑139 (MQ=255)

                                                                                                                          cGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgatctgtc                 >  3:98124/1‑134 (MQ=255)

                                                                                                                             aTCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTa               >  5:383605/1‑138 (MQ=255)

                                                                                                                              tCGTAGTTA‑TCTACACGACGGGGAGTCAGGCCACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTACCTGTCAGACCAAGTTTACTCATATATACTTAAGATTGATTaaa              >  6:39895/1‑138 (MQ=255)

                                                                                                                                 tAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGt                                                     <  2:278046/96‑1 (MQ=255)

                                                                                                                                 tAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        <  1:221756/109‑1 (MQ=255)

                                                                                                                                 tAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACt          >  3:107138/1‑139 (MQ=255)

                                                                                                                                 tAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACt          >  6:307045/1‑139 (MQ=255)

                                                                                                                                 tAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACt          <  8:407263/139‑1 (MQ=255)

                                                                                                                                   gTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACtt         >  6:22015/1‑138 (MQ=255)

                                                                                                                                      a‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                                          <  8:120236/102‑1 (MQ=255)

                                                                                                                                      a‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                                          <  4:128471/102‑1 (MQ=255)

                                                                                                                                      a‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                                          <  7:217382/102‑1 (MQ=255)

                                                                                                                                      a‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatata                                >  5:156293/1‑112 (MQ=255)

                                                                                                                                      a‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCAtt     >  1:762/1‑139 (MQ=255)

                                                                                                                                        tCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                                           <  4:164736/68‑1 (MQ=255)

                                                                                                                                         cTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAg                                                                                                <  8:267637/46‑1 (MQ=255)

                                                                                                                                         atACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGATATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCAtttt   <  3:258634/138‑1 (MQ=255)

                                                                                                                                          tACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCa                                              <  3:402797/95‑1 (MQ=255)

TTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA‑TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTT  >  Exported/4511‑4786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`