BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	6,006	T→G	5.5%	V40G (GTT→GGT)	lacZalpha →	/vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	6,006	0	T	G	5.5%	16881.8 / 176.8	6772	V40G (GTT→GGT)	lacZalpha	/vntifkey=4
Reads supporting (aligned to +/- strand): ref base T (3026/3198); new base G (470/48); total (3518/3254)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 6823 total aligned reads displayed.

ATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTAC  >  Exported/5868‑6144

 tttCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGtt                                                                                                                                           >  3:80790/1‑139 (MQ=255)

    cacacaGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGAATCTAGAGGATCACCGGGTACAGAGCTCGAATTCACTGGACGTCGTTTTTACACGTAGTGAC‑TTGGAAAACCCTGGCGGTatc                                                                                                                                        >  4:244018/1‑137 (MQ=255)

     acacaGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTAcc                                                                                                                                        <  3:231527/138‑1 (MQ=255)

     acacaGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTAcc                                                                                                                                        <  5:371743/138‑1 (MQ=255)

      cacaGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGGCGTGAC‑TGGGAACACCCGGGCGTTcccc                                                                                                                                       >  4:80816/1‑138 (MQ=255)

       acacGAAACAGCTAGGACCATGATTCCGCCAAGCTTGCATGCCTGCAGGGCGACTCTAGAGGATCCCCGGGTACCCAGCTCGAATACACTGGCCGTCGTTTTACAACGTCGTTAC‑TGGGAAAACCCTGGCGTTACCCaa                                                                                                                                     <  4:275959/139‑1 (MQ=255)

         aGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAA‑AGGGAAAACCCAGGCGTTACCCAAca                                                                                                                                   >  4:108839/1‑138 (MQ=255)

            aaaCAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTaa                                                                                                                                <  3:361051/139‑1 (MQ=255)

             aaCAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAAt                                                                                                                               <  6:339534/139‑1 (MQ=255)

             aaCAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAAt                                                                                                                               <  4:362169/139‑1 (MQ=255)

               cAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCg                                                                                                                             <  8:172908/139‑1 (MQ=255)

               cAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCg                                                                                                                             <  4:18343/139‑1 (MQ=255)

               cAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCg                                                                                                                             <  2:355125/139‑1 (MQ=255)

               cAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCg                                                                                                                             <  8:115469/139‑1 (MQ=255)

                 gCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGcc                                                                                                                           <  5:212266/139‑1 (MQ=255)

                     tGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTgc                                                                                                                       <  7:67925/139‑1 (MQ=255)

                     tGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTg                                                                                                                        >  2:15865/1‑138 (MQ=255)

                       aCCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAAt                                                                                                                               <  2:40967/129‑1 (MQ=255)

                       aCCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTgcag                                                                                                                     <  5:113031/139‑1 (MQ=255)

                       aCCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTgcag                                                                                                                     >  7:186133/1‑139 (MQ=255)

                            gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACAt                                                                                                                >  1:285652/1‑139 (MQ=255)

                            gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACAt                                                                                                                >  7:47749/1‑139 (MQ=255)

                            gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAAACGCCTTGCAGCCCAt                                                                                                                >  1:92291/1‑139 (MQ=255)

                            gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCCTTTTACAACGTCGGGAC‑GGGGAAAACCCTGGCGTTAACCAACTTAAACGCCTTgcagccgat                                                                                                                >  4:50051/1‑135 (MQ=255)

                               tACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGGTTTAAAACGTCGTGAC‑TGGGGCAACCCTGGCGTTACCCCACTTAATCGCCTTGCAGcacagacc                                                                                                             >  2:284610/1‑135 (MQ=255)

                                   ccAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCACCTTAATCGCCTTGCAGCACCTCCCCCtt                                                                                                         >  7:266163/1‑139 (MQ=255)

                                      aGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTgc                                                                                                                       >  8:33497/1‑122 (MQ=255)

                                      aGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAA‑GGGGAAAACCCAGGCGGTTCCCAACTTAAAAGCCTTGCAGCACAACCCCCATTCg                                                                                                      >  1:128234/1‑139 (MQ=255)

                                       gCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTaa                                                                                                                                <  5:95720/112‑1 (MQ=255)

                                       gCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGGTTTACAACGTCGTGGC‑TGGGGAAACCCTGGCGGTTCCCAACTTAATCGCCGTGGAGCCCCTCCCCCGTTCGc                                                                                                     >  7:150586/1‑139 (MQ=255)

                                            cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                 <  4:208548/138‑1 (MQ=255)

                                            cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                 <  6:5795/138‑1 (MQ=255)

                                            cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCt                                                                                                <  5:187926/139‑1 (MQ=255)

                                                ccTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCg                                                                                            <  3:267834/139‑1 (MQ=255)

                                                ccTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCg                                                                                            <  8:206114/139‑1 (MQ=255)

                                                ccTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAAAGGCGTGAC‑TGGGGAAAACCTGGCGTTACCCCACGTAAACGCCGTGCCGCACCACCCCCGTTCGCCCGCggggg                                                                                            >  2:125481/1‑137 (MQ=255)

                                                 cTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑GGGGAAAACCCTGGCGGTACCCCACTTACTCGCCGTGCAGCCCCTCCCCCTTTCGCCCGCgggggg                                                                                           >  5:315914/1‑136 (MQ=255)

                                                    cAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTa                                                                                          <  1:15865/137‑1 (MQ=255)

                                                     agaccGACTCTAGAGGATCCCCGGGTACCGAGCTCGCATTCACTGGGCGTCGTTTTACATCGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATa                                                                                       <  1:190959/135‑1 (MQ=255)

                                                          gtctctAGAGGATCCCCGGGTACGGAGGTCGAATTCACTGGACGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCATTTCGCCTGCTGTCGAAATAGGGat                                                                                  <  6:392575/137‑2 (MQ=255)

                                                          gACTCTAGAGGCTCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACCACGTCGTGTC‑AGGGAAAACCCTGGCGGTACCCACAGTAAACGCCGTGCAGCCCATCCCCCATTAGCCCGCTGGCCTAATatccta                                                                                  >  5:344108/1‑134 (MQ=255)

                                                          gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                  <  3:150250/139‑1 (MQ=255)

                                                          gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                  <  3:198188/139‑1 (MQ=255)

                                                          gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                  <  5:28510/139‑1 (MQ=255)

                                                          gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                  <  1:225625/139‑1 (MQ=255)

                                                           aCTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTAAAACCACGATGC‑AGGGGAAAACCAGGCGTTAGCCAAAGATATCGCCGTGCCCCCCCTCCCCCATTCGCCACCTGGCGGAATAGCGAag                                                                                 >  4:159773/1‑139 (MQ=255)

                                                           aCTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGGTTTTCAACGGCGGGGCGTGGGAAAA‑CCGGGCGGTAGCCCACTTAATCGCCATGCAGCACCAGCCCCTTTCGCCCGGCGGCGTGATATCGCag                                                                                 >  8:233464/1‑139 (MQ=255)

                                                                     aTCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGTAAACCCAGGCGTTACCCAACTTAATAGCCTTTGAGCACAGACCCCCTTAGCCAGCTGGGGGTAAAGGGAAGAGGccccccc                                                                       >  1:401036/1‑135 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTTCAACGTCGGGGC‑TGGGGAAACCCAGGCGGTTCCCCACTTAATAGCCTTGCAGCACATCCCCCTTTCCCCCGCTGGCGGAATTGCGCACAGGcccccccc                                                                      >  7:319472/1‑134 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGTC‑TGGGAAAACCCTGGCGGTACCCAACTTTATCGCCTTGCCGCCCCTACCCCGTTAGCCCGCTGGCGTAATAGCGAAGACGCCCGcccc                                                                      >  6:186540/1‑139 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGGCTTGCCGCCCATCCCCCTTTCGCCCGCTGGCGTCATAGCGAAGAGGcccccccc                                                                      >  8:300119/1‑134 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCCGCCCATCCCCCTTTCGCCCGCTGGCCTAATAGCGAAGAGGCCCGcccc                                                                      >  8:254860/1‑139 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                  >  6:369997/1‑127 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCGTGCAGCCCCGCCCCCTTTCGCCAGCTGGCGGAATAGCGAAGAGGCCCGCAcc                                                                      >  8:390870/1‑139 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGGTACCCCACTTAATCGCCTTGCAGCACATCCCCCGTTCGCCAGCGGGGGGAATAGCGAAGAGGCCCGcccc                                                                      >  6:287487/1‑139 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑AGGGAAAAACCTGGCCTTACCCAACGTAATCGACGAGCAGCACCACCCCCATTCGCCCGCACGCGGAATAACGAAGAGGCCCGcccc                                                                      >  6:209871/1‑139 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTAAAACGTAGTGAC‑TGGGAAAACCCTGGCGTTAACCCACATAATAGCCATGCAGCACCTCCCCCATTATCCAGCTGGGGGTATAGCGAAGAGGCCCGcccc                                                                      >  2:313730/1‑139 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGGTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCCACATAAAAGCCTTGCAGCACAACCCCCATTCGCCAGCAGGCGGTATAGCGAAGAGGCCCGCAcc                                                                      >  2:95639/1‑139 (MQ=255)

                                                                      cccccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTTCCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCAcc                                                                      >  5:166922/2‑139 (MQ=255)

                                                                       ccccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAAACCAGGCGTTACCCCACATAATCGCCTTTCAGCACATCCCCCAATCCCCAACAGGCGTAATAGCACACAGGCCCGCACCg                                                                     >  6:148668/1‑139 (MQ=255)

                                                                       ccccGGGTACCGAGCTCGAATTCACTGGCCGTCGGTTTACAACGGCGTGGC‑TGGGCACACCCTGGCGGGTCCCCCCTTAATCGCCGTGCCGCCCCTACCCCGTTCGCCCGCAGGCGGAATAGCGAAGAGGCCCCCCCCg                                                                     >  5:246075/1‑139 (MQ=255)

                                                                         ccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGAt                                                                   <  3:329804/139‑1 (MQ=255)

                                                                           gggTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCg                                                                 <  7:9261/139‑1 (MQ=255)

                                                                             gTACCGAGCTCGATTTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCTTAATAGCGAAGAGGCCCGCACCGATCGcc                                                               <  1:320675/139‑1 (MQ=255)

                                                                             gTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCTTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGcc                                                               <  5:55730/139‑1 (MQ=255)

                                                                             gTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGcc                                                               <  8:363917/139‑1 (MQ=255)

                                                                             gTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCCCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGcc                                                               <  6:246548/139‑1 (MQ=255)

                                                                                   aGCTCGAATTCCCTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTccc                                                         <  3:38986/139‑1 (MQ=255)

                                                                                   aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGGC‑GGGGGAAACCCGGGCGGTGCCCCACTTAATCGCCGTGCAGCCCCTCCCCCGTTCGCCCGCTGGGGGAATAGCGAAGCGGCCCGCCCCGCTCGCCCGTccc                                                         >  6:99855/1‑139 (MQ=255)

                                                                                   aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGCC‑TGGGAAAACCCTGGCGTTACCCAACTTAATAGCCTTGCAGCACATACCCCTTTCGCCAGCAGGCGTAATAGCGAAGACGCCCCCACCGATCGCCCTTcc                                                          >  5:393696/1‑138 (MQ=255)

                                                                                   aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCCCATCCCCCTTTCGCCCGCTGGCGTAATAGCGAAGAGGCCCGCACCGCTCGCCCTTccc                                                         >  3:46174/1‑139 (MQ=255)

                                                                                   aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTccc                                                         <  5:145973/139‑1 (MQ=255)

                                                                                   aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTccc                                                         <  3:29439/139‑1 (MQ=255)

                                                                                   aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGGACTAGCGAAGAGGCCCGCACCGATCGCCCTTccc                                                         >  7:217505/1‑139 (MQ=255)

                                                                                       cGAATTCACTGGCCGTCGTTTTACAACGTCGTGCC‑TGGGAAAACCCTGGCGGTTCCCCCCGTAATCGCCGTGCCGCACATCCCCCGTTCGCCCCCGGGCGGTATAGCGAAGAGGCCCCCCCCCGTCGCCCGTGccacca                                                     >  4:135453/1‑139 (MQ=255)

                                                                                               cTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCCGCCCATCCCCCGTTCGCCACCTGGCGGAATAGCGAAGAGGCCCCCCCCCCTCGCCCATCCCCACAGTTGCGCa                                             >  3:120770/1‑139 (MQ=255)

                                                                                               cTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCATTCGCCAGCTGGCGGAATATCGAAGAGGCCCCCACCGATAGCCCATCCCCACAGTTGCCCa                                             >  1:100097/1‑139 (MQ=255)

                                                                                                  gCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTAcc                                                                                                                                        >  7:240304/1‑45 (MQ=255)

                                                                                                  gCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGc                                           <  7:126855/138‑1 (MQ=255)

                                                                                                                 cgtcgtGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATAGCCTTGCAGCACATCCCCCATTCCCCCGCTGGCGTAAAAGCGAAGAGGCCCCCCCCGATCGCCCTTCCCAACACTTTCGCAGCCTgaaaggcgaatggc                           >  6:316421/1‑139 (MQ=255)

                                                                                                                   tcgtGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTgaatgg                                   <  1:257367/129‑1 (MQ=255)

                                                                                                                   tcgtGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACACCCCCCTTTCGCCAGCTGGCGTAATAGCGATGAGGCCCGCCCCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGgcgc                         >  6:125914/1‑139 (MQ=255)

                                                                                                                    cgtGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGcc                        <  7:387381/139‑1 (MQ=255)

                                                                                                                             ggAAAACCCTGGCGTTACCCAACTTACTCGCCTTGCAGCACATCCCCCATTCGCCAGCTGGCGTAATAGCGCAGAGGCCCGCACCGATCGCCCTTCCCAACAGTAGCGCAGCCTGAATGGCGAATGGCGCCTGATGc                  <  2:230773/137‑1 (MQ=255)

                                                                                                                             ggAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGca                 <  8:47749/138‑2 (MQ=255)

                                                                                                                              gAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCa                                                        >  6:171590/1‑98 (MQ=255)

                                                                                                                              gAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGt               >  1:359203/1‑139 (MQ=255)

                                                                                                                                 aagccTGGCGTTAGCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTAtt            <  8:140848/136‑1 (MQ=255)

                                                                                                                                 aaCCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCACTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGGCTGATGCGGTAtt            <  8:271986/139‑1 (MQ=255)

                                                                                                                                  aCCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                 >  2:258830/1‑53 (MQ=255)

                                                                                                                                   cccTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATa                                                                                       <  7:353032/62‑1 (MQ=255)

                                                                                                                                   cccTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTAtttt          <  4:305660/139‑1 (MQ=255)

                                                                                                                                   cccTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTAtttt          <  2:66125/139‑1 (MQ=255)

                                                                                                                                   cccTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTAtttt          >  4:332335/1‑139 (MQ=255)

                                                                                                                                     cTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCCCCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTtct        >  8:69679/1‑139 (MQ=255)

                                                                                                                                     cTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTtct        <  4:386807/139‑1 (MQ=255)

                                                                                                                                       ggCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGcc                                                               >  2:199693/1‑82 (MQ=255)

                                                                                                                                       ggCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCGGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGc                                           >  3:177264/1‑102 (MQ=255)

                                                                                                                                        gCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTcc      <  7:29152/138‑1 (MQ=255)

                                                                                                                                        gCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCGGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTtctc       <  1:63719/137‑1 (MQ=255)

ATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC‑TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTAC  >  Exported/5868‑6144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`