BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	6,256	A→C	5.1%	intergenic (+45/–)	lacZalpha → / –	/vntifkey=4/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	6,256	0	A	C	5.1%	16948.2 / 121.2	6996	intergenic (+45/–)	lacZalpha/–	/vntifkey=4/–
Reads supporting (aligned to +/- strand): ref base A (3208/3238); new base C (444/19); total (3727/3269)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 7142 total aligned reads displayed.

GCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTT  >  Exported/6118‑6394

    cTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACggg                                                                                                                                        <  1:51852/139‑1 (MQ=255)

       aagaacaaatcgctcgaggaaattgaaaagctttggataaaatgaaaaaaagcgcagctgaaatagctgcgcttttttgtgtCATAATTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCtt                                                                                                                                     >  5:400660‑M2/83‑139 (MQ=255)

                      cTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCgg                                                                                                                         <  6:29821/136‑1 (MQ=255)

                      cTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCa                                                                                                                       <  4:133461/138‑1 (MQ=255)

                      cTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCa                                                                                                                       <  8:316790/138‑1 (MQ=255)

                       ttACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGCCACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATc                                                                                                                     >  7:191544/1‑139 (MQ=255)

                           gCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCt                                                                                                                 <  4:100521/139‑1 (MQ=255)

                             aTCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTa                                                                                                               <  2:225257/139‑1 (MQ=255)

                                    cGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACaa                                                                                                        >  4:396396/1‑139 (MQ=255)

                                       tATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATc                                                                                                                     >  4:258611/1‑123 (MQ=255)

                                         tttCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCtgt                                                                                                   >  8:408330/1‑139 (MQ=255)

                                         tttCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCtgt                                                                                                   >  7:104835/1‑139 (MQ=255)

                                            cacaCCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTACGCCAGCCCCGACACCCGCCACCACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTCCAGACAAGCTGTGa                                                                                                 <  5:94266/138‑1 (MQ=255)

                                              cacCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCg                                                                                              <  4:364632/139‑1 (MQ=255)

                                                 cGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTc                                                                                                                                  >  2:29025/1‑100 (MQ=255)

                                                   cATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTcc                                                                                         <  3:25914/139‑1 (MQ=255)

                                                   cATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTcc                                                                                         >  3:112169/1‑139 (MQ=255)

                                                   cATATGGTGCACTCACAGAACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCATGTCATCTCCCCGCCTCAGCATACAGACAACCTGTGAccctgta                                                                                          >  2:355208/1‑133 (MQ=255)

                                                           gCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGCCAAGCTGTGGCCGTCTCCGGGAGCTg                                                                                 >  3:335827/1‑139 (MQ=255)

                                                           gCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGCCAAGCTGTGACCGTCTCCGGGAGCTg                                                                                 >  6:144488/1‑139 (MQ=255)

                                                           gCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCg                                                                                        <  6:354980/132‑1 (MQ=255)

                                                             aCTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCa                                                                               <  5:191066/139‑1 (MQ=255)

                                                               tctcAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGCCGGGCTTGTCTGCTCCCCGCCTCCCCATACACACCAGCTGTGACCGTCGCCCGGCGCTGCAtg                                                                             >  7:279965/1‑139 (MQ=255)

                                                               tctcAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCGGCCCGGCTTGTCATCTACCGGCATCAGCTTACAGACAAGCTCTGACCGACTCCGGGAGCGGCAtg                                                                             >  7:307213/1‑139 (MQ=255)

                                                                 tcAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGc                                                                                   >  8:142891/1‑131 (MQ=255)

                                                                  cAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCAGACCGGCATGTCTGCACCCGGCATCCGCGAACACACCACCAGAGGCCGTCACCGGGAgcagcaagggg                                                                          >  1:188715/1‑134 (MQ=255)

                                                                   aGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGa                                                                                                 >  5:219986/1‑115 (MQ=255)

                                                                   aGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCAtgt                                                                            <  6:387186/136‑1 (MQ=255)

                                                                   aGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATgtgt                                                                          >  5:294256/1‑138 (MQ=255)

                                                                   aGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCATGTCTGCTCCCGGCATCCGCATAAAGCCAAGCGGTGACCGTCTCCGGGAGCTGCATgtgt                                                                          >  6:174132/1‑138 (MQ=255)

                                                                         aTCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGAc                                                                                                          <  1:362929/100‑1 (MQ=255)

                                                                         aTCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGa                                                                                                 <  3:389397/109‑1 (MQ=255)

                                                                         aTCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGt                                                                                             <  1:153939/113‑1 (MQ=255)

                                                                         aTCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGt                                                                   >  5:138483/1‑139 (MQ=255)

                                                                         aTCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCGGCCGGGCTTGTCTGCTCCCCGCCTCCGCTTACAGACCAGCTGTGCCCGTCTCCCGGAGCGGCATGTGTCAGAGGt                                                                   >  2:11296/1‑139 (MQ=255)

                                                                          tCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGCCCGTCTCCGGGAGCTGCATGTGTCAGAGGtt                                                                  >  3:198104/1‑139 (MQ=255)

                                                                           cTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAg                                                                                    >  3:356811/1‑120 (MQ=255)

                                                                             gCTCTGATGCCGCATAGTTAAGCCAGCCCCGCCACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACCAGCTGTGACCGTCTCCGGGCGCTGCATGTGTCAGAGGtttt                                                                >  5:374740/1‑138 (MQ=255)

                                                                             gCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGCCGGGCTTGTCTGCTCCCGGCCTCCGCTTACAGCCAAGCGGTGACCGTCTCCGGGCGCGGCCTGTGTGCGAGGtttt                                                                >  5:252323/1‑138 (MQ=255)

                                                                             gCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATc                                                                                                                     >  1:221249/1‑85 (MQ=255)

                                                                             gCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGAcc                                                                                               >  3:148867/1‑107 (MQ=255)

                                                                             gCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCAGACGGGCTTTTCTGCTCCCGGCATCCGCTTAAAGACCAGCTGTGACCGTCTCCGGGCGCTGCATGAGTCCGAGGtttt                                                                >  2:84494/1‑138 (MQ=255)

                                                                               tctGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGGTTGTATGCTCCCCGCCTCCGCTTACAGACCAGCTGTGACCGTGTCCGGGAGCTGCATGTGTCAGAGGttttacc                                                             >  1:335040/1‑136 (MQ=255)

                                                                               tctGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCAt                                                                                                                      >  3:311412/1‑82 (MQ=255)

                                                                               tctGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCAc                                                             >  8:113814/1‑139 (MQ=255)

                                                                               tctGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCAGTGACCGTCTCCGGGAGCGGCATGTGTCAGAGGGTTTAAc                                                             >  8:228695/1‑139 (MQ=255)

                                                                               tctGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTACCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCAc                                                             >  6:199411/1‑139 (MQ=255)

                                                                               tctGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTACCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCAc                                                             >  8:344876/1‑139 (MQ=255)

                                                                                 tGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACTCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCAc                                                             <  3:286715/137‑1 (MQ=255)

                                                                                   aTGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGt                                                          <  6:22736/138‑1 (MQ=255)

                                                                                   aTGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGt                                                          >  2:52613/1‑138 (MQ=255)

                                                                                    tGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGTGCTTGTCTGCTCCCGGCATCCGCt                                                                                                                 <  7:57113/82‑1 (MQ=255)

                                                                                        gCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTTTATTCTACCGGCATACGCATACAAACAAGCTGTGACCGTCACCCGGAGCAGCATTTTTCAGACGTTTTAAACGTCGTCAc                                                    >  6:118544/1‑139 (MQ=255)

                                                                                        gCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTTCTACCGGCATACCCTTAAATACAATCTCTGACCGTCTCCGGGAGCTGCATGTGTCAGACGTTTTAACCGTCAacac                                                    >  4:338632/1‑139 (MQ=255)

                                                                                        gCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCAc                                                    >  4:314575/1‑139 (MQ=255)

                                                                                        gCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCAc                                                    >  4:90344/1‑139 (MQ=255)

                                                                                        gCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCGTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCGGGCTGTGTCAGAGGTTTTCCCCGTCATCAc                                                    >  8:291880/1‑139 (MQ=255)

                                                                                         cATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCAcc                                                   >  7:83219/1‑139 (MQ=255)

                                                                                                aaGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTACCGGCCTCCGCTTACAGACAAGCTGTGACCGTCTCCGGGCGCTGCATGTGTCAGAGGGTTTCACCGTCATCACC‑AAAcgcg                                           >  3:45206/1‑139 (MQ=255)

                                                                                                 agcccgccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAAcgcg                                           <  2:188715/139‑1 (MQ=255)

                                                                                                 agccagccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACggg                                                                                                                                        >  8:382017/1‑46 (MQ=255)

                                                                                                    cagccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCga                                        <  7:369411/139‑1 (MQ=255)

                                                                                                      gccCCGACACCCGCCAACACCCGCTGACGCGCCCTGCCGGGCTTGTCTGCACCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTGCCCGTGATAACCGAAACGCGCgcga                                      >  8:173752/1‑139 (MQ=255)

                                                                                                      gccCCGACACCCGCCAACACCCGCTGACGCGCCCTGCCGGGCATGTCTGCTCCCGGCCTCCGCTTACAGACAAGCAGTGACCCTCTCCGGGCGCTGCATGTGTCAGAGGTTTTAAACGTCATCACCGAAACGCGCgcga                                      >  7:167566/1‑139 (MQ=255)

                                                                                                      gccCCGACACCCGCCAACACCCGCTGACGCGCCCTGCCGCGCTTGTCTGCTCCCGGCATCCCCTTAAAGACAAGCTGTGCACGTCTCCGGGAGGTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCgaga                                      >  5:110110/1‑139 (MQ=255)

                                                                                                      gccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGCCAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCgaga                                      >  3:171312/1‑139 (MQ=255)

                                                                                                      gccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGCGCTGCATGTGTCAGAGGTTTTCACCGtcat                                                       >  7:248638/1‑122 (MQ=255)

                                                                                                      gccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTc                                                               <  7:397972/114‑1 (MQ=255)

                                                                                                      gccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCCCCGAAACGCGCgaga                                      >  1:306234/1‑139 (MQ=255)

                                                                                                      gccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCgaga                                      >  1:27336/1‑139 (MQ=255)

                                                                                                      gccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCgaga                                      >  8:261274/1‑139 (MQ=255)

                                                                                                      gccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCgaga                                      >  4:56104/1‑139 (MQ=255)

                                                                                                      gccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCCCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCgaga                                      >  4:167286/1‑139 (MQ=255)

                                                                                                      gccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCGTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGCGCTGCATGTGTCAGAGGTTTTCCCCGTCATCACCGAAACGCGCgaga                                      >  6:327787/1‑139 (MQ=255)

                                                                                                      gccCCGACACCCGCCAACACCCGCTGACGCGCCCGGACCGGCTTTTCTTCTACCGGCCACCGCTTTAACACAACCTGTGACCGGCTCCGGGCGCGGCAAGTGTCAGCAGTTTTAGCCCGAAACACCGAAAcccgcccgc                                      >  6:262306/1‑135 (MQ=255)

                                                                                                      gccCCGACACCCGCCAACACCCGCTGACGCGCCCAGACGCGCTTGTCTGCTCCCCGCATACCCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCAGCACCGAAACGCGCgaga                                      >  7:221407/1‑139 (MQ=255)

                                                                                                       ccccGACACCCGCCAACACCCGCTGACGCGCCCTGCCGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGCCCGGCGACGGGAGCTGCATGTGTCAGCGGTTTTCACCGGCATCACCGCAAcgcgcgcgcc                                     >  1:129434/1‑135 (MQ=255)

                                                                                                       ccccGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGAc                                     >  5:346744/1‑139 (MQ=255)

                                                                                                       ccccGACACCCGCCAACACCCGCTGACGCGCCCGGCCCGGCGTGTCTGCACCCCGCCACCGCTTACACACAAGCTGTGACCGGCGCCCGGAGCTGCCTGTTTGAGAGGGTTTAACCGGCCACACCGCAACCCGCGCGAc                                     >  6:42620/1‑139 (MQ=255)

                                                                                                            acacCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAg                                >  6:82292/1‑139 (MQ=255)

                                                                                                                ccGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGcc                            <  1:247710/139‑1 (MQ=255)

                                                                                                                ccGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGcc                            <  1:396373/139‑1 (MQ=255)

                                                                                                                  gCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGGCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTc                          <  7:131648/139‑1 (MQ=255)

                                                                                                                      acacCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGa                      <  3:74453/139‑1 (MQ=255)

                                                                                                                      acacCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGa                      <  2:321234/139‑1 (MQ=255)

                                                                                                                      acacCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGa                      <  2:389401/139‑1 (MQ=255)

                                                                                                                            gCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAg                                                                                                       <  5:321728/52‑1 (MQ=255)

                                                                                                                            gCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAAcgcgc                                          <  8:198030/113‑1 (MQ=255)

                                                                                                                             cTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCt               <  1:100436/139‑1 (MQ=255)

                                                                                                                                    gcCCTGACGGGGTTGTCTGCGCCCGGCATCCGCTTACAGACAAGCTGTGACCGGCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTta        <  1:80843/139‑1 (MQ=255)

                                                                                                                                    gcCCTGACGGGCTTGTCTGCTCCCGGCATCCTCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACTAAAGGGCCTCGTGATACGCCTATTTTta        <  5:65689/139‑1 (MQ=255)

                                                                                                                                    gcCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTta        <  2:252575/139‑1 (MQ=255)

                                                                                                                                       cTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTta        <  4:228529/136‑1 (MQ=255)

                                                                                                                                       cTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTta        >  3:228529/1‑136 (MQ=255)

                                                                                                                                        taaCGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGCCGAAAGGGCCTCGTGCTACGCCTATTTTTATAgg    >  7:332565/3‑139 (MQ=255)

                                                                                                                                        tGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGcc                            <  4:28717/115‑1 (MQ=255)

                                                                                                                                          aCGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGtt  <  5:16725/139‑1 (MQ=255)

                                                                                                                                          aCGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGtt  <  8:72114/139‑1 (MQ=255)

                                                                                                                                          aCGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGt   <  4:198104/138‑1 (MQ=255)

                                                                                                                                          aCGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCGGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGCAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTTTAGGtt  >  2:145497/1‑139 (MQ=255)

GCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTT  >  Exported/6118‑6394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`