Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	1,415,681	G→A	5.1%	G425S (GGC→AGC)	U712_07270 →	Zinc‑transporting ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	1,415,681	0	G	A	5.1%	253.7 / 2.4	79	G425S (GGC→AGC)	U712_07270	Zinc‑transporting ATPase
Reads supporting (aligned to +/- strand):  ref base G (36/38);  new base A (2/2);  total (38/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.11e-01

CCAAGCCATAACGGCGTACGCTGAAAGCCGCGGCGTGAATCAGTCCGGCTACATATCTATAGAAGAAACCTCAGGGTTTGGCGTTATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGTCATACAATTGTATTTGTGAAAAAGGATGATCAAATAGCAGGCTGTATCGCACTGAAGGACCAAATCAGGCC  >  CP006881/1415545‑1415811                                                                                                                                          |                                                                                                                                   ccATGCCATAACGGGGTACGCTGAATGACGCGGCGTGAATCAGTCCGGCTACATATCTATAGAAGAAACCTCAGGGTTTGGCGTTATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑Cggg                                                                                                                                  <  8:258348/139‑2 (MQ=255) ccAAGCCATAACGGCGTACGCTGAAAGCCGCGGCGTGAATCAGTCGGGCTACATATCTATAGAAGAAACCTCAGGGTTTGGCGTTATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGc                                                                                                                                  <  3:71145/139‑1 (MQ=255) ccAAGCCATAACGGCGTACGCTGAAAGCCGCGGCGTGAATCAGTCCGGCTACATATCTATAGAAGAAACCTCAGGGTTTGGCGTTATGGCAGAAGTGTCGGGGGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGc                                                                                                                                  <  7:229452/139‑1 (MQ=255)    aGCCATAACGGCGTACGCTGAAAGCCGCGGCGGGCATCAGTCCGGCTACATATCTATAGAAGAAACCTCAGGGTGTGGCGGTATGGCAGAAGTGTCGGGGGCGAAATGGAAGGGCGGTAAAGCAGGTTTTAT‑CGGCGAg                                                                                                                               >  5:187591/1‑139 (MQ=255)           aCGGCGTACGCTGAAAGGCGCGGCGTGAATCAGTCCGTCTACATATCTATAGAAGAAACCTCAGTGTTTGGCGTTATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATgg                                                                                                                        <  7:351835/139‑1 (MQ=255)               cGTACGCTGAAAGCCGCGGCGTGAATCAGTCCGGCTACATATCTATAGAAGAAACCTCAGGGTTTGGCGTTATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGgcagc                                                                                                                    >  6:47828/1‑139 (MQ=255)               cGTACGCTGAAAGCCGCGGCGTGAATCAGTCCGGCTACATATCTATAGAAGAAACCTCAGGGTTTGGCGTTATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGgcag                                                                                                                     >  8:154122/1‑138 (MQ=255)               cGTACGCTGAAAGCCGCGGCGTGAATCAGTCCGGCTACATATCTATAGAAGAAACCTCAGGGTTTGGCGTTATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGgcag                                                                                                                     >  4:208344/1‑138 (MQ=255)                gTACGCTGAAAGCCGCGGCGTGAATCAGTCCGGCTACATATCTATAGAAGAAACCTCAGGGTTTGGCGTTATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGgcagca                                                                                                                   <  1:10317/139‑1 (MQ=255)                 tACGCTGAAAGCCGCGGCGTGAATCAGTCCGGCTACATATCTATAGAAGAAACCTCAGGGTTTGGCGTTATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGgcagca                                                                                                                   >  5:280007/1‑138 (MQ=255)                        aaaGCCGCGGCGTGAATCAGTCCGGCTACATATCTATAGAAGAAACCTCAGGGTTTGGCGTTATGGCAGAAGTGTCGGGGGGGAAAAGGAAGGGCCGTAAAGCAGGTGTTAT‑AGGCGCGGTAAAGGCAGCACAAATTAt                                                                                                           >  2:408535/1‑139 (MQ=255)                               cggcgTGAATCAGTCCCGCTACATATCTATAGAAGAAACCTCAGGGTTTTGGGTTATGGCAGAAGTGTCGTGTGCGAAATGGAAGGTAGGTTAAGCATGTTTTAT‑CGGCGAGGAAATTGCAGCACAATTTTTGaaaca                                                                                                     >  5:215789/1‑138 (MQ=255)                                 gcgTGAATCAGTCCGGCTACATATCTATAGAAGAAACCTCAGGGTTTGGCGTTATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGaaacaaac                                                                                                  <  7:154122/139‑1 (MQ=255)                                   gTGAATCAGTCCGGCTACATATCTATAGAAGAAACCTCAGGGTTTGGCGTTATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAg                                                                                                <  5:13757/139‑1 (MQ=255)                                    tGAAACAGTCCGGCTACATATCTATTGAAGAAACCTCAGGGGTTGGCGTTATGGCAGAACTGTCGGGTGCGAAAAGGAAGGTAGGTAAAGAATGATTTAT‑CGGCGAGGTAAATGCAGCACAAATTAATAaacaaacaa                                                                                                >  6:13757/1‑137 (MQ=255)                                       atctctCCGGCTACATATCTATATAAGAATGCTCAGGGTTTGGCGTTATGGCAGTAGTGTCGTGTGCGATATGGAAGGTCGGTATAGGATGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATc                                                                                            <  3:199813/134‑1 (MQ=255)                                           gTCCGGCTACATATCTATAGAAGAAACCTCAGGGTTTGGCGTTATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGAt                                                                                        <  5:148930/139‑1 (MQ=255)                                           gTCCGGCTACATATCTATAGAAGAAACCTCAGGGTTTGGCGTTATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGAt                                                                                        <  1:262080/139‑1 (MQ=255)                                            tacGGCTACATATCTATTGAAGAAACCTCAGGGTTTGGCGGTATGGCAGAAGTGTCGGGTGCCAAATGGAAGGTACGGAAAGCACGATTTATACGGC‑AAGAAATGGCAGCACAATTTATGAAACAAAAATCATAAGaag                                                                                       >  2:164256/3‑137 (MQ=255)                                                      tatCTATAGAAGAAACCTCAGGGTTTGGCGTTATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAg                                                                             >  1:357293/1‑139 (MQ=255)                                                         cTATAGAAGAAACCTCAGGGTTTGGCGTTATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCgg                                                                          >  2:95290/1‑139 (MQ=255)                                                          tataGAAGAAACCTCAGGGTTTGGCGTTATGGCAGAAGTGTCGGGTGCGTAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGt                                                                         >  3:236969/1‑139 (MQ=255)                                                           ataGAAGAAACCTCAGGGTTTGGCGTTATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGt                                                                         >  3:290548/1‑138 (MQ=255)                                                           ataGAAGAAACCTCAGGGTTTGGCGTTATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGt                                                                         >  6:27178/1‑138 (MQ=255)                                                           ataGAAGAAACCTCAGGGTTTGGCGTTATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGt                                                                         >  7:101260/1‑138 (MQ=255)                                                            taGAAGAAACCTCAGGGTTTGGCGTTATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTTT‑AGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGACATTCAACGCGGTc                                                                        >  4:287952/1‑138 (MQ=255)                                                            taGAAGAAACCTCAGGGTTTGGCGTTATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGaaacaaac                                                                                                  <  3:43919/112‑1 (MQ=255)                                                            taGAAGAAACCTCAGGGTTTGGCGTTATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGaaacaaac                                                                                                  >  4:43919/1‑112 (MQ=255)                                                                 gaaACCTCAGGGTTTGGCGTTATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑AGGCGAGGAAATGGCAGCAAAAATTATGAAACAAACAGCATAAGATGTCATTAAAAGCGGACATACa                                                                   >  2:135146/1‑138 (MQ=255)                                                                    aCCTCAGGGTTTGGCGTTATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAg                                                                             <  5:150692/125‑1 (MQ=255)                                                                    aCCTCAGGGTTTGGCGTTATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAg                                                                             >  6:150692/1‑125 (MQ=255)                                                                    aCCTCAGGGTTTGGCGTTATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGTCATACAATTg                                                               >  7:53318/1‑139 (MQ=255)                                                                    aCCTCAGGGTTTGGCGTTATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGTCATACAATTg                                                               >  1:246949/1‑139 (MQ=255)                                                                    aCCTCAGGGTTTGGCGTTATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CAGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGTCATACAATTg                                                               >  6:212993/1‑139 (MQ=255)                                                                    aCCTCAGGGTTTGGCGTTATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CAGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGTCATACAATTg                                                               >  6:218399/1‑139 (MQ=255)                                                                    aCCTCAGGGTTTGGCGTTATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑AGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGTCATACAATTg                                                               >  7:16588/1‑139 (MQ=255)                                                                         aGGGTTTGTCGTTATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGTCATACAATTGTAttt                                                          >  1:280053/1‑139 (MQ=255)                                                                         aGGGTTTGGCGTTATGGCAGAAGTGTCGGGTGCGATATTGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGTCATACAATTGTAt                                                            <  2:280053/137‑1 (MQ=255)                                                                               tGGCGTTATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGa                                                                                                                             >  1:79071/1‑66 (MQ=255)                                                                               tGGCGTTATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGa                                                                                                                             <  2:79071/66‑1 (MQ=255)                                                                               tGGCGTTATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGaaacaaac                                                                                                  <  2:47580/93‑1 (MQ=255)                                                                               tGGCGTTATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGaaacaaac                                                                                                  >  1:47580/1‑93 (MQ=255)                                                                               tGGCGTTATGGAATAAGTGTCGGGGGCGAAATGGAAGGTCGGTAAAGCTGGTTTTAG‑CGGCGAGGAAATGGCAGGACAATTTATGAAACAAACCGCATCATATGTCTTTCAAGGCGGTCATACAATTGTTTTTGTGat                                                     >  6:363656/1‑137 (MQ=255)                                                                                ggCGTTATGGCAGAAGTGTCGGGTGTGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGTCATACAATTGTATTTGTGaaa                                                    >  6:111745/1‑138 (MQ=255)                                                                                ggCGTTATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTTT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGTCATACAATTGTATTTGTGaaa                                                    >  5:64820/1‑138 (MQ=255)                                                                                ggCGTTATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCaa                                                                               <  8:162026/111‑1 (MQ=255)                                                                                ggCGTTATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCaa                                                                               >  7:162026/1‑111 (MQ=255)                                                                                ggCGTTATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGTCATACAATTGTATTTGTGaaa                                                    <  5:27178/138‑1 (MQ=255)                                                                                ggCGTTATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGTCATACAATTGTATTTGTGaaa                                                    >  5:410628/1‑138 (MQ=255)                                                                                ggCGTTATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGTCATACAATTGTATTTGTGaaa                                                    >  7:191587/1‑138 (MQ=255)                                                                                     tATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGTCATACAATTGTATTTGTGAAAAAGgat                                              >  4:133652/1‑139 (MQ=255)                                                                                      aTGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGTCATACAATTGTATTTGTGAAAAAGgat                                              <  3:133652/138‑1 (MQ=255)                                                                                         gCAGAAGTGTCGGGTGCGATATGGAAGGGCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCACAGCGGTCATACAATTGTATTTGTGAAAAAGGATGATc                                          <  4:236969/139‑1 (MQ=255)                                                                                         gCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGTCATACAATTGTATTTGTGAAAAAGGATGATc                                          <  8:101260/139‑1 (MQ=255)                                                                                          cAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGTCATACAATTGTATTTGTGAAAAAGGATGATCa                                         <  2:246949/139‑1 (MQ=255)                                                                                              aGTGTCGGGTGTGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGTCATACAATTGTATTTGTGAAAAAGGATGATCAAATa                                     <  5:111745/139‑1 (MQ=255)                                                                                               gtgtCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAAGAGCATCAGATGTCATTCAAAGCGTTCATACAATTGTATTTGTGAAAAATGATGATCAAATAg                                    <  3:287952/139‑1 (MQ=255)                                                                                               gtgtCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGTCATACAATTGTATTTGTGAAAAAGGATGATCAAATAg                                    <  2:276037/139‑1 (MQ=255)                                                                                                  tCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGTCATACaa                                                                  >  6:235902/1‑106 (MQ=255)                                                                                                  tCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGTCATACaa                                                                  <  5:235902/106‑1 (MQ=255)                                                                                                  tCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGTCATACAATTGTATTTGTGAAAAAGGATGATCAAATAGCAg                                 >  5:368093/1‑139 (MQ=255)                                                                                                  tCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGTCATACAATTGTATTTGTGAAAAAGGATGATCAAATAGCAg                                 >  3:124858/1‑139 (MQ=255)                                                                                                   cGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGTCATACAATTGTATTTGTGAAAAAGGATGATCAAATAGCAgg                                <  5:47828/139‑1 (MQ=255)                                                                                                   cGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGTCATACAATTGTATTTGTGAAAAAGGATGATCAAATAGCAgg                                <  8:191587/139‑1 (MQ=255)                                                                                                   cGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CAGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGTCATACAATTGTATTTGTGAAAAAGGATGATCAAATAGCAgg                                <  5:212993/139‑1 (MQ=255)                                                                                                    gggTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGTCATACAATTGTATTTGTGAAAAAGGATGATCAAATAGCAgg                                <  3:208344/138‑1 (MQ=255)                                                                                                    gggTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CAGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGTCATACAATTGTATTTGTGAAAAAGGATGATCAAATAGCAgg                                <  5:218399/138‑1 (MQ=255)                                                                                                        gCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAATCAAACAGCATCAGATGTCATTCAAAGCGGTCATACAATTGTATTTGTGAATAAGGATGATCAAATAGCAGGCTGTa                           <  4:124858/139‑1 (MQ=255)                                                                                                         cGAAATGGAAGGTCGGTAAAGCAGGGTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGTCATACAATTGTATTTGTGAAAAAGGATGATCAAATAGCAGGCTGTAt                          <  4:290548/139‑1 (MQ=255)                                                                                                           aaaTGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAACTGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGTCATACAATTGTATTTGTGAAAAAGGATGAGCAAATAGCAGGCTGTATCg                        <  6:187591/139‑1 (MQ=255)                                                                                                                 aaGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGTCATACAATTGTATTTGTGAAAAAGGATGATCAAATAGCAGGCTGTATCGCACTGa                  <  1:171092/139‑1 (MQ=255)                                                                                                                  aGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGTCATACAATTGTATTTGTGAAAAAGGATGATCAAATAGCAGGCTGTATCGCACTGaa                 <  1:95290/139‑1 (MQ=255)                                                                                                                      cGGTAAAGCAGGTTTTTT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGTCATACAATTGTATTTGTGAAAAAGGATGATCAAATAGCAGGCTGTATCGCACTGAAGGAc             <  6:64820/139‑1 (MQ=255)                                                                                                                       ggTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGTCATACAATTGTATTTGTGAAAAAGGATGATCAAATAGCAGGCTGTATCGCACTGAAGGAc             <  4:75072/138‑1 (MQ=255)                                                                                                                                 gctatat‑aGGCGAGGAAAAGGCAGCACAATTTATGAAACAAACAGCAACACTTGTCATTCAAACAGGTCATACAATTGTAGTTGAGAAAAAGGATGATCATAAAGCAGTCTGTATCGCACTGAATGACAAAATCAGccc  <  6:349835/135‑1 (MQ=255)                                                                                                                                 gTTTTAT‑CTGCGACGAAATGGCAGCACAATTTATGAAACATACAGCATCAGAGTGCTTTCTAAGTGGTCATACAATTTTATTTGTGAAAAAGGATGAGCAAATACCAGGATGTATCGCACTGAAGGACCAAATCAGGcc  <  8:231135/139‑1 (MQ=255)                                                                                                                                 gTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGTCATACAATTGTATTTGTGAAAAAGGATGATCAAATa                                     >  1:304555/1‑104 (MQ=255)                                                                                                                                 gTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGTCATACAATTGTATTTGTGAAAAAGGATGATCAAATa                                     <  2:304555/104‑1 (MQ=255)                                                                                                                                 gTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGTCATACAATTGTATTTGTGAAAAAGGATGATCAAATATCAGGCTGTATCGCACTGAATGACCAAATCAGGcc  <  1:164256/139‑1 (MQ=255)                                                                                                                                  ttttAT‑CGGCGAGGATATGGCAGCACAATTTATGAAACAAACATCATCAGATGTCATTCAAAGCGGTCATACAATTGTATTTGTGAAAAAGTATGATCAAATAGCAGGCTGTATCGCACTGAAGGACCAAATCAGGcc  <  1:135146/138‑1 (MQ=255)                                                                                                                                          |                                                                                                                                   CCAAGCCATAACGGCGTACGCTGAAAGCCGCGGCGTGAATCAGTCCGGCTACATATCTATAGAAGAAACCTCAGGGTTTGGCGTTATGGCAGAAGTGTCGGGTGCGAAATGGAAGGTCGGTAAAGCAGGTTTTAT‑CGGCGAGGAAATGGCAGCACAATTTATGAAACAAACAGCATCAGATGTCATTCAAAGCGGTCATACAATTGTATTTGTGAAAAAGGATGATCAAATAGCAGGCTGTATCGCACTGAAGGACCAAATCAGGCC  >  CP006881/1415545‑1415811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑