Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 1,973,569 A→C 23.6% L385R (CTT→CGT)  U712_09760 ← Putative transporter yoaB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068811,973,5690AC23.6% 63.0 / 3.4 44L385R (CTT→CGT) U712_09760Putative transporter yoaB
Reads supporting (aligned to +/- strand):  ref base A (16/16);  new base C (2/9);  total (18/26)
Fisher's exact test for biased strand distribution p-value = 8.56e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTAAAAAGATAAAAAGCGCCCCTCAAACAGGGCGCTTTCTAATTAAGATGCATATTGAGGAGATGTTTCCTCCTTAACTGCTTTTTCCTCAGGTATATGAATACATTTTGTCAAGACCGCACTTGCAAGGTAAAGGGCCGCAAAGATCCAGACGACACCTTGCGCGCCGACGAGACCGATGAACAGCCAAGCGAGTGCAGGGCCGACAAACGCTGATAAACCTGCAGCTAAATTCAATACAGACATGGCTGCCCCTTTATCTTTTCCTGCC  >  CP006881/1973435‑1973706
                                                                                                                                      |                                                                                                                                         
tttAAAAAGATAAAAAGCGCCCCTCAAACAGGGCGCTTTCTAATTAAGATGCATATTGAGGAGATGTTTCCTCCTTAACTGCTTTTTCCTCAGGTATATGAATACATTTTGTCAAGACCGCACTTGCAAGGTAAAGGGc                                                                                                                                       <  3:390370/139‑1 (MQ=255)
    aaaaGATAAAAAGCGCCCCTCAAACAGGGCGCTTTCTAATTAAGATGCATATTGAGGAGATGTTTCCTCCTTAACTGCTTTTTCCTCAGGTATATGAATACATTTTGTCAAGACCGCACTTGCAAGGTAAAGGGCCGCa                                                                                                                                   <  8:287252/139‑1 (MQ=255)
    aaaaGATAAAAAGCGCCCCTCAAACAGGGCGCTTTCTAATTAAGATGCATATTGAGGAGATGTTTCCTCCTTAACTGCTTTTTCCTCAGGTATATGAATACATTTTGTCAAGACCGCACTTGCAAGGTAAAGGGCCGCa                                                                                                                                   <  4:93147/139‑1 (MQ=255)
    aaaaGAAAAAAAGCGCCCCTCAAACAGGGCGCTTTCTAATTAAGATGCATATTGAGGAGATGTTTCCTCCTTAACTGCTTTTTCCTCAGGTATATGAATACATTTTGTCAAGACCGCACTTGCAAGGTAAAGGGCCGCa                                                                                                                                   <  5:272333/139‑1 (MQ=255)
     aaaGATAAAAAGCGCCCCTCAAACAGGGCGCTTTCTAATTAAGATGCATATTGAGGAGATGATTCCTCCTTAACTGCTTTTTCCTCAGGTATATGAATACATTTTGTCAAGCCCGCACTTGCAAGGTAAAGGGCCGCaa                                                                                                                                  <  7:230479/139‑1 (MQ=255)
          tAAAACGCGCCCCTCAAACAGGGCGCTTTCTAATTAAGATGCATATTGAGGAGATGTTTCCTCCTTAACTGCTTTTTCCTCAGGTATATTAATACTTTTTGTCAAGACCGCACTTGAAAGGTAAAGGGCCGCAAAGAt                                                                                                                              <  7:98396/138‑1 (MQ=255)
                   ccccTCAAACAGGGCGCTTTCTAATTAAGATGCATATTGAGGAGATGTTTCCTCCTTAACTGCTTTTTCCTCAGGTATATGAATACATTTTGTCAAGACCGCACTTGCAAGGTAAAGGGCCGCAAAGATCCAGACGaca                                                                                                                    >  1:350897/1‑139 (MQ=255)
                   caccTCAAACAGGGCGCTTTCTAATTAAGATGAATATTGAGGAGATGTTTCCTCCTTAACTGATTTTTCCTCAGGTATTTGAATACATTTTGTCTCGACAGCACTTGCAAGGTAACTGGCCGCAAAGAACCAGACGaca                                                                                                                    >  5:338227/3‑139 (MQ=255)
                               ggCGCTTTCTAATTAAGATGCATATTGAGGAGATGTTTCCTCCTTAACTGCTTTTTCCTCAGGTATATGAATACATTTTGTCAAGACCGCACTTGCAAGGTAAAGGGCCGCAAAGATCCAGACGACACCTTGCGCGCcg                                                                                                        >  5:368703/1‑139 (MQ=255)
                                   cTTTCTAATTAAGATGCATATTGAGGAGATGTTTCCTCCTTAACTGCTTTTTCCTCAGGTATATGAATACATTTTGTCAAGACCGCCCTTGCAAGGTATAGGGCCGCAAAGATCCAGACGACACCTTGCGCGCcgac                                                                                                      >  4:323433/1‑137 (MQ=255)
                                       cTAATTAAGATGCATATTGAGGAGATGTTTCCTCCTTAACTGCTTTTTCCTCAGGTATATGAATACATTTTGTCAAGACCGCACTTGCAAGGTAAAGGGCCGCAAAGATCCCGACGACACCTTGCGCGCCGACGAGAc                                                                                                 <  7:120351/138‑1 (MQ=255)
                                             aaGATGCAAATTGAGGAGATGTTGCCTCCTTAACTGCTTTTTCCTCAGGTATATTAATAAATTTTGTCAAGCCCGCACTTGCAAGGTAACGGGCCGCAAAGATCCACACGACACCTTGCGCGCCGACGAGACCGATGa                                                                                           <  7:219726/138‑1 (MQ=255)
                                              aGATGCATATTGAGGAGATGTTTCCTCCTTAACTGCTTTTTCCTCAGTTATAGGAATACATTTTGTCACGACCGCACTTGCAAGGTAACGGGCCGAAAAGATCCAGACGACACCTTGCGCGCCGACGAGACCGATGAAc                                                                                         <  8:222882/139‑1 (MQ=255)
                                                aTGCATATTGAGGAGATGTTTCCTCCTTAACTGCTTTTTCCTCAGGTATATGAATACATTTTGTCAAGACCGCACTTGCAAGGTAAAGGGCCGCAAAGATCCAGACGACACCTTGCGCGCCGCCGAGACCGATGAACAg                                                                                       >  1:302616/1‑139 (MQ=255)
                                                aTGCATATTGAGGAGATGTTTCCTCCTTAACTGCTTTTTCCTCAGGTATATGAATACATTTTGTCAAGACCGCACTTGCAAGGTAAAGGGCCGCAAAGATCCAGACGACACCTTGCGCGCCGACGAGACCGATGAACAg                                                                                       >  3:198695/1‑139 (MQ=255)
                                                 tGCATATTGAGGAGATGTTTCCTCCTTAACTGCTTTTTCCTCAGGTATATGAATACATTTTGTCAACACCGCACTTGCAAGGTAACGGGCCGCAAAGATCCAGACGACACCTTGCGCGCCGACGAGACCGATGAACAGc                                                                                      <  1:289513/139‑1 (MQ=255)
                                                       ttGAGGATATGTTTCCTACTTAACTGCTTTTTCGTCAGGTATATGAAAACATTTTGTCAAGACCGCACTTGCAAGGTAACGGGCCGCAAAGATCCAGACGACACCTTGCGCGCCGACGAGACCGATGAACAGCCAAGc                                                                                 <  8:61966/138‑1 (MQ=255)
                                                       ttGAGGAGATGTTTCCTCCTTAACTGCTTTTTCCTAAGGTATATGAATACATTTTGTCAAGACCTCACTTGCAAGGTAACGGGCCGCAAAGATCCAGACGACACCTTGCGCGCCGACGAGACCGATGAACAGCCAAGc                                                                                 <  8:250476/138‑1 (MQ=255)
                                                         gaggagATGTTTCCTCCTTAACTGCTTTTTCCTCAGGTATATGAATACATTTTGTCAAGACCGCACTTGCAAGGTAAAGGGCCGCAAAGATCCAGACGACACCTTGCGCGCCGACGAGACCGATGAAc                                                                                         <  6:127938/128‑1 (MQ=255)
                                                         gaggagATGTTTCCTCCTTAACTGCTTTTTCCTCAGGTATATGAATACATTTTGTCAAGACCGCACTTGCAAGGTAAAGGGCCGCAAAGATCCAGACGACACCTTGCGCGCCGACGAGACCGATGAAc                                                                                         >  5:127938/1‑128 (MQ=255)
                                                          aggagATGTTTCCTCCTTAACTGCTTTTTCCTCAGGTATATGAATACATTTTGTCAAGACCGCACTTGCAAGGTATAGGGCCGCAAAGATCCAGACGACACCTTGCGCGCCGACGAGACCGAGGAACAGCCAAGCGa                                                                               >  7:381017/1‑137 (MQ=255)
                                                            gagaTGTTTCCTCCTTAACTGCTTTTTCCTCAGGTATATGAATACATTTTGTCAAGACCGCACTTGCAAGGTAAAGGCCCGCAAAGATAAAGACGACACCTTGCGCGCCGACGAGACCGATGAACATCCAAGCGAGTg                                                                            >  8:13303/1‑138 (MQ=255)
                                                              gaTGTTTCCTCCTTAACTGCTTTTTCCTCAGGTATATGAATACATTTTGTCAAGACCGCACTTGCAAGGTAAAGGGCCGCAAAGATCCAGACGACACCTTGCGCGCCGAGGAGACCGATGAACAGCCAAGCGAGTGCAg                                                                         >  1:110820/1‑139 (MQ=255)
                                                               aTGTTTCCTCCTTAACTGCTTTTTCCTCAGGTATATGAATACATTTTGTCAAGACCGCACTTGCAAGGTAAAGGGCCGCAAAGATCCAGACGACACCTTGCGCGCCGACGAGACCGATGAACAGCCAAGCGAGTGCAgg                                                                        >  5:322537/1‑139 (MQ=255)
                                                                    tcctccTTAACTGCTTTTTCCTCAGGTATATGAATACATTTTGTCAAGACCGCACTTGCAAGGTAAAGGGCCGCAAAGATCCAGACGACACCTTGCGCGCCGACGAGACCGATGAACAGCCAAGCGAGTGCAGGGCCGa                                                                   >  1:203794/1‑139 (MQ=255)
                                                                      ctcctTAACTGCTTTTTCCTCAGGTATATGAATACAATTTGTCAAGACCGAACTAGCAAGGTAAAGCGCCGCAAAGATCCAGACGACACATTGCGAGCCGAATAGACCGATCAACAGCCACGCGAGTGCAGGGCCGAc                                                                  >  3:303747/1‑138 (MQ=255)
                                                                         ctTAACTGCTTTTTCCTAAGGTATATTAAAAAATTTTGTCAAGACGGCACTTGAAAGGTAAAGGGCCTCAAAGATCCAGACGACACCTTGCGCGCCGACGAGACCGATGAACAGCCAAGCGAGTGCAGGGCCGACaa                                                                <  2:350897/137‑1 (MQ=255)
                                                                               tGCTTTACCCACAGTTATATAAAAAATTTTTGCAAACCCCCCCCTTGCACGGAAACGGCCCCCAACCAGCCCCACGACACCTTGCGCGCCGACGAGACCGATGAACAGCCAAGCGAGTGCAGGGCCGACAAACGCTGa                                                         <  2:203794/138‑1 (MQ=255)
                                                                                 cTTTTTCCTCAGGTATATCAATACATTTTGTCAACACCCCACTTGCAAGGTAAAGGGCCGCAAAGACCCAGACGACACCTTGCGCGCCGACGAGACCGATGAACAGCCAAGCGAGTGCAGGGCCGACAAACGCTGAt                                                        <  3:133038/137‑1 (MQ=255)
                                                                                     ttCCTCAGGTATATGAATACATTTTGTCAAGACCGCACTTGCAAGGTAAAGGGCCGCAAAGATCCAGACGACACCTTGCGCGCCGACGAGACCGATGAACAGCCAAGCGAGTGCAGGGCCGACAAACGCTGATAAAcc                                                   <  6:177178/138‑1 (MQ=255)
                                                                                                tatCAATACATTTTGTATAGACGGCAGTGTCAAGGTATTGGCCCTCAAAGCTCCAGACGACACCTTGCGCGCCGACTAGACCGATGAACATCCAAGCGAGTGCAGGGCCGACAAACGCTGATAAACCTGCATCTAAttt                                       <  6:338227/139‑1 (MQ=255)
                                                                                                  tGAATACATTTTGTCAAGACCGCACTTGCAAGGTAAAGGGCCGCAAAGATCCAGACGACACCTTGCGCGCCGACGAGACCGATGAACAGCCAAGCGAGTGCAGGGCCGACAAACGCTGATAAACCTGCAGCTAAATTc                                      >  1:340055/1‑138 (MQ=255)
                                                                                                    aacaCATTTTGTCAAGACCGCCCTTGCAAGGAAACGGCCCGAAAAGATCCAGACGACACCTTGCGCGCCGACGAGACCGATGAACAGCCAAGCGAGTGCAGGGCCGACAAACGCTGATAAACCTGCAGCTAAATTCAAt                                   <  8:381017/136‑1 (MQ=255)
                                                                                                     aTACATTTTGTCACTCCCCCACTTGCATGGTAACGGGCCCCAAAGAGCCAGACGACACCTTGCGCGCCGACGAGACCGATGAACAGCCAAGCGAGTGCAGGGCCGACAAACGCTGATAAACCTGCAGCTAAATTCAAt                                   <  3:254613/138‑1 (MQ=255)
                                                                                                       aCATTTTGTCAAGACCGCACTTGCAAGGTAAAGGGCCGCAAAGATCCAGACGACACCTTGCGCGCCGACGAGACCGATGAACAGCCAAGCGAGTGCAGGGCCGACAAACGCTGATAAACCTGCAGCt                                            <  2:230100/127‑1 (MQ=255)
                                                                                                       aCATTTTGTCAAGACCGCACTTGCAAGGTAAAGGGCCGCAAAGATCCAGACGACACCTTGCGCGCCGACGAGACCGATGAACAGCCAAGCGAGTGCAGGGCCGACAAACGCTGATAAACCTGCAGCt                                            >  1:230100/1‑127 (MQ=255)
                                                                                                         aTTTTGTCAAGACCGCACTGGCAAGGTACAGGGCCGCAAAGAACCCGCCGACACCTTGCGCGCCGACGAGACCGATGAACAGCCAAGCGAGTGCAGGGCCGACAAACGCTGATAAACCTGCAGCTAAATTCAATACAGa                              <  5:2253/139‑1 (MQ=255)
                                                                                                             tGTCAAGACCGCACTTGCAAGGTAAAGGGCCGCAAAGATCCAGACGCCACCTTGCGCGCCGACGAGACCGATGAACAGCCAAGCGAGTGCAGGGCCGACCAACGCTGATAAACCTGCAGCTAAATTCAATACAGACATg                          >  4:79455/1‑139 (MQ=255)
                                                                                                                 aaGCCCGCACTTGCAAGGTAAAGGGCCGCAAAGATCCAGACGACACCTTGCGCGCCGACGAGACCGATGAACAGCCAAGCGAGTGCAGGGCCGACAAACGCTGATAAACCTGCAGCTAAATTCAATACAGACATGGCTg                      <  2:110820/139‑1 (MQ=255)
                                                                                                                 aaGACCGCTATTGCATGGTAAAGGGCCGCAAATAGCCAGACGACACCTTGCGCGCCGACGAGACCGATGAACAGCCAAGCGAGTGCAGGGCCGACAAACGCTGATAAACCTGCAGCTAAATTCAATACAGACATGGCTg                      <  4:312722/139‑1 (MQ=255)
                                                                                                                 aaGACCGCACTTGCAATGTAAAGGGCCGCAAAGATCCAGACGACACCTTGCGCGCCGACGAGACCGATGAACAGCCAAGCGAGTGCAGGGCCGACAAACGCTGATAAACCTGCAGCTAAATTCAATACAGACATGGCTg                      <  7:13303/139‑1 (MQ=255)
                                                                                                                          cTTGCAAGGTAACGGCCCGCAACTATCCTGACGACACCTTGCGCGCCGACGAGACCGATGAACAGCCAAGCGAGTGCAGGGCCGACAAACGCTGATAAACCTGCAGCTAAATTCAATACAGACATGGCTGCCCCTTTAt             <  6:322537/139‑1 (MQ=255)
                                                                                                                             gCAAGGTAACGGGCCGCAAAGATCCAGACGACACCTTGCGCGCCGACGAGACCGATGAACAGCCAAGCGAGTGCAGGGCCGACAAACGCTGATAAACCTGCAGCTAAATTCAATACAGACATGGCTGCCCCTTTATCtt          >  8:245983/1‑139 (MQ=255)
                                                                                                                              cAAGGTAAAGGGCCGCAAAGATCCAGACGACACCTTGCGCGCCGACGAGACCGATGAACAGCCAAGCGAGTGCAGGGCCGACAAACGCTGATAAACCTGCAGCTAAATTCAATACAGACATGGCTGCCCCTTTATCgtt         >  1:166908/1‑139 (MQ=255)
                                                                                                                                     aaGGGCCGCAAAGATCCCGACGACACCTTGCGCGCCGACGAGACCGATGAACAGCCAAGCGAGTGCAGGGCCGACAAACGCTGATAAACCTGCAGCTAAATTCAATACAGACATGGCTGCCCCTTTATCTTTTCCTGcc  <  6:354878/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                         
TTTAAAAAGATAAAAAGCGCCCCTCAAACAGGGCGCTTTCTAATTAAGATGCATATTGAGGAGATGTTTCCTCCTTAACTGCTTTTTCCTCAGGTATATGAATACATTTTGTCAAGACCGCACTTGCAAGGTAAAGGGCCGCAAAGATCCAGACGACACCTTGCGCGCCGACGAGACCGATGAACAGCCAAGCGAGTGCAGGGCCGACAAACGCTGATAAACCTGCAGCTAAATTCAATACAGACATGGCTGCCCCTTTATCTTTTCCTGCC  >  CP006881/1973435‑1973706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑