| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 2,204,721 | T→G | 11.1% | K274T (AAA→ACA) | U712_11055 ← | Stage IV sporulation protein A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 2,204,721 | 0 | T | G | 11.1% | 129.3 / 2.7 | 58 | K274T (AAA→ACA) | U712_11055 | Stage IV sporulation protein A |
| Reads supporting (aligned to +/- strand): ref base T (22/29); new base G (5/2); total (27/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCATATAGATGATCAGGCGCGTACAAATCAATTTCTGCCACCCCTTGGCCCAGCTCAATTCCGGCTAATCCGGCACTTTCAATGAATTCAAACTCGCTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑TTAATATCCTTAACCGTTTCCTTCACGGACTCCTGATAGCTTTCACGCAACCAATGGTTTTCTTTCAGCACCATTACCCAGCTTGGGAGATTGACATTCACTTCTAGCACCGGAAACTCGTAGAGGGCCTCTC > CP006881/2204586‑2204853 | tCATATAGATGATCAGGCGCGTACAAATCAATTTCTGCCACCCCTTGGCCCAGCTCAATTCCGGCTAATCCGGCACTTTCAATGAATTCAAACTCGCTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑TTaa < 7:54709/139‑1 (MQ=255)tCATATAGATGATCAGGCGCGTACAAATCAATTTCTGCCACCCCTTGGCCCAGCTCAATTCCGGCTAATCCGGCACTTTCAATGAATTCAAACTCGCTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑TTa < 1:161385/138‑1 (MQ=255) atatAGATGATCAGGCGCGTACAAATCAATTTCTGCCACCCCTTGGCCCAGCTCAATTCCGGCTAATCCGGCACTTTCAATGAATTCAAACTCGCTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑TTAat < 3:14454/138‑1 (MQ=255) tataGATGATCAGGCGCGTACAAATCAATTTCTGCCACCCCTTGGCCCAGCTCAATTCCGGCTAATCCGGCACTTTCAATGAATTCAAACTCGCTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑TTAata > 3:55468/1‑138 (MQ=255) tataGATGATCAGGCGCGTACAAATCAATTTCTGCCACCCCTTGGCCCAGCTCAATTCCGGCTAATCCGGCACTTTCAATGAATTCAAACTCGCTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑TTAata > 2:241510/1‑138 (MQ=255) ataGATGATCAGGCGCGTACAAATCAATTTCTGCCACCCCTTGGCCCAGCTCAATTCCGGCTAATCCGGCACTTTCAATGAATTCAAACTCGATGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑GTAgtat > 5:14374/1‑138 (MQ=255) tCAGGCGCGTACAAATCAATTTCTGCCACCCCTTGGCCCAGCTCAATTCCGGCTAATCCGGCACTTTCAATGAATTCAAACTCGCTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑TTAATATCCTTAACCg > 1:85462/1‑139 (MQ=255) cAGGCGCGTACAAATCAATTTCTGCCACCCCTTGGCCCAGCTCAATTCCGGCTAATCCGGCACTTTCAATGAATTCAAACTCGCTGAATTGGCCGCAAACCCTGTCTACGTCCCGGAGCCGT‑TTAATAAACTTAACCga > 7:364616/1‑138 (MQ=255) cAGGCGCGTACAAATCAATTTCTGCCACCCCTTGGCCCAGCTCAATTCCGGCTAATCCGGCACTTTCAATGAATTCAAACTCGCTGAATTGGCCGACACCCCTGTCTACGTCCCGGAGCCGT‑TGAATATGCTTagaact > 1:234258/1‑134 (MQ=255) cAGGCGCGTACAAATCAATTTCTGCCACCCCTTGGCCCAGCTCAATTCCGGCTAATCCGGCACTTTCAATGAATTCAAACTCGCTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑TTa > 7:31547/1‑125 (MQ=255) cAGGCGCGTACAAATCAATTTCTGCCACCCCTTGGCCCAGCTCAATTCCGGCTAATCCGGCACTTTCAATGAATTCAAACTCGCTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑TTa < 8:31547/125‑1 (MQ=255) cAGGCGCGTACAAATCAATTTCTGCCACCCCTTGGCCCAGCTCAATTCCGGCTAATCCGGCACTTTCAATGAATTCAAACTCGCTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑TTAATATCCTTAAACGt > 6:372972/1‑139 (MQ=255) cAGGCGCGTACAAATCAATTTCTGCCACCCCTTGGCCCAGCTCAATTCCGGCTAATCCGGCACTTTCAATGAATTCAAACTCGCTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑GTAATATCCTTAACCGt > 1:86446/1‑139 (MQ=255) aGGCGCGTACAAATCAATTTCTGCCACCCCTTGGCCCAGCTCAATTCCGGCTAATCCGGCACTTTCAATGAATTCAAACTCGCTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑TTAATATCCTTAACCGt < 1:249391/138‑1 (MQ=255) gcgcGTACAAATCAATTTCTGCCACCCCTTGGCCCAGCTCAATTCCGGCTAATCCGGCACTTTCAATGAATTCAAACTCGCTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑TTAATATCCTTAACCGt < 7:363072/136‑1 (MQ=255) gcgcGTACAAATCAATTTCTGCCACCCCTTGGCCCAGCTCAATTCCGGCTAATCCGGCACTTTCAATGAATTCAAACTCGCTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑TTAATATACTTAACCGt > 8:363072/1‑136 (MQ=255) aaTCAATTTCTGCCACCCCTTGGCCCAGCTCAATTCCGGCTAATCCGGCACTTTCAATGAATTCAAACTCGCTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑TTAATATCCTTAACCGTTTCCTTCACGGa < 1:27162/139‑1 (MQ=255) aaTCAATTTCTGCCACCCCTTGGCCCAGCTCAATTCCGGCTAATCCGGCACTTTCAATGAATTCAAACTCGCTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑TTAATATCCTTAACCGTTTCCTTCACGGa < 4:55468/139‑1 (MQ=255) ctaggCCCAGCTCAATTCCGGCGAATCCCGCACTTTCAATGAATTCACACTCGCTGACTTGGCCGACAACCCTGGCTACGTCCCGGAGCCGC‑TAAAAATCCTAAACCGACACCTTCACGGACTCCTGATAGCTATCACg < 6:239074/136‑1 (MQ=255) aGCTCAATTCCGGCTAATCCGGCACTTTCAATGAATTCAAACTCGCTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑GTAATATCCTTAACCGGTTGCTTCACGGACTCCTGATATCTTTAACGCAACCa > 6:378580/1‑137 (MQ=255) aGCTCAATTCCGGCTAATCCGGCACTTTCAATGAATTCAAACTCGCTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGACGT‑TTAATATCCTTAACCGTTTCCTTCACGGACTCCTGATAGCTTTCACGCAACCAAt < 8:295734/139‑1 (MQ=255) gCTCAATTCCGGCTAATCCGGCACTTTCAATGAATTCAAACTCGCTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑TTAATATCCTTAACCGTTTCCTTCACGGACTCCTGATAGCTTTCACGCAACCAATg > 1:27675/1‑139 (MQ=255) cTCAATTCCGGCTAATCCGGCACTTTCAATGAATTCAAACTCGCTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑TTAATATCCTTAACCGTTTCCTTCACGGACTCCTGATAGCTTTCACGCAACCAATgg > 8:124582/1‑139 (MQ=255) cTCAATTCCGGCTAATCCGGCACTTTCAATGAATTCAAACTCGCTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑TTAATATCCTTAACCGTTTCCTTCACGGACTCCTGATAGCTTTAACGCAACCAATgg > 7:288259/1‑139 (MQ=255) cTCAATTCCGGCTAATCCGGCACTTTCAATGAATTCAAACTCGCTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑TTAATATCCTTAACCGTTTACTTCACGGCCGCCTGGTAGCGTTCCCGCCACCAAAgg > 7:214529/1‑139 (MQ=255) gCTAATCCGGCACTTTCAATGAATTCAAACTCGCTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑TTAat > 6:125059/1‑77 (MQ=255) gCTAATCCGGCACTTTCAATGAATTCAAACTCGCTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑TTAat < 5:125059/77‑1 (MQ=255) gCTAATCCGGCACTTTCAATGAATTCAAACTCGCTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑TTAATATCCATAACCCTTTCCTTCACGGACGCCTGATAGCTTTAACGCAACCAATtgttttgttt > 7:206345/1‑137 (MQ=255) cTAATCCGGCACTTTCAATGAATTCAAACTCGCTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑TTAATATCCTTAACCGTTTCCTTCACGGACTCCTGATAGCTTTCACGCAACCAATGGTTTTCTTTCAg < 6:362056/139‑1 (MQ=255) aTCCGGCACTTTCAATGAATTCAAACTCGCTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑TTAATATCCTTTACCGTTTCCTTCACGGACGCCTGATAGCTTTCACGCAACCAATGGTTTTCTTTCAGCAc > 5:139568/1‑139 (MQ=255) tacGGCACTTTCAATGAATTCAAACTCGCTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGTGTTAAT‑TCACAAAAAGTTTCAATGAAGGCCTCCAGAAAGAATTCACACAAACAATGGGTATTTTTCAacaca > 2:99559/3‑138 (MQ=255) tCCGGCACTTTCAATGAATTCAAACTCGCTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑TTTATATCCATAAACGGTTACTTAACGGACGCCTGATAGCTTTAACGCAACCAATTGTTTTGTTTCAGCAcc > 4:305379/1‑139 (MQ=255) tCCGGCACTTTCAATGAATTCAAACTCGCTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑TTAATATCCTTAACCGTTTCCTTCACGGACTCCTGATAGCTTTCACGCAACCAATGGTTTTCTTTCAGCAc > 8:37898/1‑138 (MQ=255) gCACTTTCAATGAATTCAAACTCGCTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑TTAATATCCTTAACCGTTTCCTTCACGGACTCCTGATAGCTTTCACGCAACCAATGGTTTTCTTTCAGCACCATTa < 6:33119/139‑1 (MQ=255) acattCAATGAATTCAAACTCGCTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑TTAATATCCTTAACCGTTTCCTTCACGGACTCCTGATAGCTTTCACGCAACCAATGGTTTTCTTTCAGCACCATTAcc < 2:9347/136‑1 (MQ=255) aCTTTCAATGAATTCAAACTCGCTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑TTAATATCCTTAACCGTTTCCTTCACGGACTCCTGATAGCTTTCACGCAACCAATGGTTTTCTTTCAGCACCATTAcc < 5:378580/139‑1 (MQ=255) aCTTTCAATGAATTCAAACTCGCTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑TTAATATCCTTAACCGTTTCCTTCACGGACTCCTGATAGCTTTCACGCAACCAATGGTTTTCTTTCAGCACCATTAcc < 6:55191/139‑1 (MQ=255) aCTTTCAATGAAATCAAACTCGCTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑TTAATATCCTTAACCGTTTCCTTCACGGACTCCTGATAGCTTTCACGCAACCAATGGTTTTCTTTCAGCACCATTAcc < 8:364616/139‑1 (MQ=255) ttCAAACTCGCTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑GTAATATCCTTAACCGGTTGCTTCACGGCCACCGGATAGCTTTAACCCAACCAATGGTTTTATTTAAGCACCATTACCCAGCTTgg > 5:94590/1‑135 (MQ=255) cTCGCTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑TTAATATCCTTAACCGTTTCCTTCACGGACTCCTGATAGCTTTCACGCAACCAATGGTTTTCTTTCAGCACCATTACCCAGCTTGGGAGATTGACa < 2:86446/139‑1 (MQ=255) gCTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑TTAATATCCTTAACCGTTTCCTTCACGGACTCCTGATAGCTTTCACGCAACCAATGGTTTTCTTTCAGCACCATTACCCAGCTTGGGAGATTGACAt < 1:241510/137‑1 (MQ=255) gCTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑TTAATATCCTTAACCGTTTCCTTCACGGACTCCTGATAGCTTTCACGCAACCAATGGTTTTCTTTCAGCACCATTACCCAGCTTGGGAGATTGACAt < 7:37898/137‑1 (MQ=255) cTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑TTAATATCCTTAACCGTTTCCTTCACGGACTCCTGATAGCTTTCACGCAACCAATGGTTTTCTTTCAGCACCATTTCCCAGCTTGGGAGATTGACATTCa > 7:31732/1‑139 (MQ=255) cTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑TTAATATACATAACCGGTTACGTCCCGGTCTCCTGATAGCTTTCACGCACCCAAAGGTTTTGTTTAAGCCCCATTACCCAGCTTGGGAGATTTACAGTCa > 4:338582/1‑139 (MQ=255) cTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑GTTATATACTTAACCGTTTCCGTCACGGACGCCTGATAGCTTTAACGCAACCAATGGTTTTGTTTCAGCCCCAATTACCAGCGTGGGGGATTGTCAttaa > 5:234330/1‑137 (MQ=255) aaCCCTGTCTACGTCCCGGAGCCGT‑TTAATATCCTTAACCGTTTCCTTCACGGACTCCTGATAGCTTTCACGCAACCAATGGTTTTCTTTCAGCACCATTACCCAGCTTGGGAGATTGACATTCACTTCTAGCACCGGa < 5:302760/139‑1 (MQ=255) aaCCCTGTCTACGTCCCGGAGCCGT‑GTAATATCCTTAACCGTTTCCATCACGGACTCCTGATAGCTTTCACGCAACCAATGGTTTTCTTTCAGCACCATTACCCAGCTTGGGAGATTGACATTCACTTCTAGCACCGGa < 6:14374/139‑1 (MQ=255) aaCCCTGTCGACGTCCCGGAGCCGA‑TTAATATCCTTAACCGTGTCCGTCACGGACTCCTGATAGCTTTCACGCAACCAATGGTTTTCTTTCAGCACCATTACCCAGCTTGGGAGATTGACATTCACTTCTAGCACCGGa < 2:287961/139‑1 (MQ=255) tGTCTACGTCCCGGAGCCGT‑TTAATATCCTTAACCGGTTCCTTCACGGACTCCTGATAGCTTTAACGCAACCAAAGGTTTTCTTTCAGCACCATTACCCAGCTTGGGAGATTGACATTCACTTCTAGCACCGGAAACTc > 8:119713/1‑139 (MQ=255) cTACGGCCCGGAGCCGT‑TTAATAACCTTACCCGTTACCTTCACGGACTCCTGATAGCTTTCCCGAAACCAATGGTTTTATTACAGCACAATTACCCTGCTTGGGAGATTGAAATTCAATTCTAGCACCGGAAACGCGTa < 5:372972/139‑1 (MQ=255) tACGTCCCGGAGCCGT‑TTAATATCCTTAACCGTTTGCTTCACGGACTCCTGATAGCTTTCACGCAACCAATGGTTTTCTTTAAGCACCATTACCCAGCGTGGGAGCTTGACATTCACTTCTAGCACCGGAAACTCGTag > 8:70825/1‑139 (MQ=255) cGTCCCGGAGCCGT‑TTAATATCCTTAACCGTTTCCTTCACGGACTCCTGATAGCTTTCACGCAACCAATGGTTTTCTTTCAGCACCATTACCCAGCTTGGGAGATTGACATTCACTTCTAGCACCGGAAACTCGTaga < 7:124582/138‑1 (MQ=255) cGTCCCGGAGCCGT‑TTAATATCCTTAACCGTTTCCTTCACGGACTCCTGATAGCTTTCACGCAACCAATGGTTTTCTTTCAGCACCATTACCCAGCTTGGGAGATTGACATTCACTTCTAGCACCGGAAACTCGTa < 3:305379/136‑1 (MQ=255) cGTCCCGGAGCCGT‑TTAATATCCTTAACCGTTTCCATCACGGACTCCTGATAGCTTTCACGCAACCAATGGTTTTCTTTCAGCACCATTACCCAGCTTGGGAGATTGACATTCACTTCTAGCACCGGAAACTCGTaga < 6:234330/138‑1 (MQ=255) gTCCCGGATCCCT‑GTAATATCCGTAACCGTTTCCTTCACGGACTCCTGCTAGCTTGCACGCAACCAATTGTGTTCTTTCAGCACCATTACCCAGCTTGGGAGATTGACATTCACTTCAAGCACCGGAAACTCGTAGAgg < 2:372719/139‑1 (MQ=255) tCCCGGAGCCGT‑TTAATATCCTTAACCGTTTCCTTCACGGACTCCTGATAGCTTTCACGCAACCAATGGTTTTCTTTCAGCACCATTACCCAGCTTGGGAGATTGACATTCACTTCTAGCACCGGAAACTCGTAGAggg < 5:351401/139‑1 (MQ=255) cccGGAGGCGG‑TTAATATCCATAACCGTATCCTTCACGGTCTCCTGATAGCTTTCACGCAACCAATGGTTTTCTTTCAGCACCATTACCCAGCTTGGGAGATTGACATTCACTTCTAGCACCGGAAACTCGTAGAGGGc < 2:234258/139‑1 (MQ=255) aGCCGT‑TTAATATCCTTAACCGTTTCCTTCACGGACTCCTGATAGCTTTCACGCAACCAATGGTTTTCTTTCAGCACCATTACCCAGCTTGGGAGATTGACATTCACTTCTAGCACCGGAAACTCGTAGAGGGCctctc < 8:206345/139‑1 (MQ=255) | TCATATAGATGATCAGGCGCGTACAAATCAATTTCTGCCACCCCTTGGCCCAGCTCAATTCCGGCTAATCCGGCACTTTCAATGAATTCAAACTCGCTGAATTGGCCGACAACCCTGTCTACGTCCCGGAGCCGT‑TTAATATCCTTAACCGTTTCCTTCACGGACTCCTGATAGCTTTCACGCAACCAATGGTTTTCTTTCAGCACCATTACCCAGCTTGGGAGATTGACATTCACTTCTAGCACCGGAAACTCGTAGAGGGCCTCTC > CP006881/2204586‑2204853 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |