Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 2,500,381 A→G 5.4% L119L (TTA→CTA)  U712_12785 ← Uncharacterized protein yqbG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,500,3810AG 5.4% 226.9 / 3.7 74L119L (TTA→CTA) U712_12785Uncharacterized protein yqbG
Reads supporting (aligned to +/- strand):  ref base A (28/42);  new base G (2/2);  total (30/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

ATCTTCAACCGGCACCCCGAATTTTTGCTTTCTATTTTCTTTTTTCTCCTGCAGATGGTAAATGTCACATCTGTGAGTCAATAAGGATTGATAACTCATATTGATCTCATCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGAAGAGAACTGCCATCCCCTAGAGTATAAGAATAGTCACCAATTTTTTCAGTTGTATATCCTTTAATAATTGACTCGTCG  >  CP006881/2500245‑2500517
                                                                                                                                        |                                                                                                                                        
aTCTTCAACCGGCACCCCGAATTTTTGCTTTCTATTTTCTTTTTTCTCCTGCAGATGGTAAATGTCACATCTGTGAGTCAATAAGGATTGATAACTCATATTGATCTCATCCGCACTTTCGCTTCAATCCCTTCTAAAt                                                                                                                                        <  7:326827/139‑1 (MQ=255)
    tCAACCGGCACCCCGAATTTTTGCTTTCTATTTTCTTTTTTTTCCTGCAGATGGTAAATGTCACATCTGTGAGTCAATAAGGATTGATAACTCATATTGATCTCATCCGCACTTTCGCTTCAATCCCTTCTAAAt                                                                                                                                        <  1:330518/135‑1 (MQ=255)
    tCAACCGGCACCCCGAATTTTTGCTTTCTATTTTCTTTTTTTTCCTGCAGATGGTAAATGTCACATCTGTGAGTCAATAAGGATTGATAACTCATATTGATCTCATCCGCACTTTCGCTTCAATCCCTTCTAAAt                                                                                                                                        >  2:330518/1‑135 (MQ=255)
            cacaCCGAATTTATGCTATCTATTTTCTTTTTTCTCCTGCAGATGGTAAATGTCACATCTGTGAGTCAATAAGGATTGATAACTCATATTGATCTCATCCGCACTTTCGCTTCAATCACTTCTAAATCATGGTCAGcag                                                                                                                            >  6:193211/1‑137 (MQ=255)
            cACCCCGAATTTTTGCTTTCTATTTTCTTTTTTCTCCTGCAGATGGTAAATGTCACATCTGTGAGTCAATAAGGATTGATAACTCATATTGATCTCATCCGCACTTTCGCTTCAATCCCTTCTAGATCAGGGTCAGCCg                                                                                                                            >  1:321902/1‑139 (MQ=255)
            cACCCCGAATTTTTGCTTTCTATTTTCTTTTTTCTCCTGCAGATGGTAAATGTCACATCTGTGAGTCAATAAGGATTGATAACTCATATTGATCTCATCCGCACTTTCGCTTCAATCCCTTCTAGATCAGGGTCAGCCg                                                                                                                            >  5:284394/1‑139 (MQ=255)
            cACCCCGAATTTTTGCTTTCTATTTTCTTTTTTCTCCTGCAGATGGTAAATGTCACATCTGTGAGTCAATAAGGATTGATAACTCATATTGATCTCATCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCg                                                                                                                            >  3:130545/1‑139 (MQ=255)
            cACCCCGAATTTTTGCTTTCTATTTTCTTTTTTCTCCTGCAGATGGTAAATGTCACATCTGTGAGTCAATAAGGATTGATAACTCATATTGATCTCATCCGCACTTTCGCTTCAAACCCTTCTAAATCAGGGTCAGCCg                                                                                                                            >  6:340991/1‑139 (MQ=255)
                ccGAATTTTTGCTTTCTATTTTCTTTTTTCTCCTGCAGATGGTAAATGTCACATCTGTGAGTCAATAAGGATTGATAACTCATATTGATCTCATCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGttt                                                                                                                        <  3:374035/139‑1 (MQ=255)
                    aTTTTTGCTTTCTATTTTCTTTTTTCTCCTGCAGATGGTAAATGTCACATCTGTGAGTCAATAAGGATTGATAACTCATATTGATCTCATCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTcaca                                                                                                                    <  4:130545/139‑1 (MQ=255)
                          gCTTTCTATTTTCTTTTTTCTCCTGCAGATGGTAAATGTCACATCTGTGAGTCAATAAGGATTGATAACTCATATTGATCTCATCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATc                                                                                                               <  4:329319/138‑1 (MQ=255)
                          gCTTTCTAATTTCTTTTTTCTCCTGCAGATGGTAAATGTCACATCTGTGAGTCAATAAGGATTGATAACTCATATTGATCTCATCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATc                                                                                                               <  2:401759/138‑1 (MQ=255)
                              gcTATTTTCTTTTTTCTCCTGCAGATGGTAAATGTCACATCTTTGAGTCAATAAGGATTGATAACTCATATTGATCTCATCCGCACTTTCTCTTCAATCCCTTCTAAATCATGCTCAGCCGGTTTCACATAATCTTtta                                                                                                          <  3:395796/138‑1 (MQ=255)
                                    ttCTTTTTTCTCCTGCAGATGGTAAATGTCACATCTGTGAGTCAATAATGATTGATAACTCATATTGATCTCATCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAAt                                                                                                     <  8:405372/138‑1 (MQ=255)
                                    ttCTTTTTTCTCCTGCAGATGGTAAATGTCACATCTGTGAGTCAATAAGGATTGATAACTCATATTGATCTCATCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAAt                                                                                                     <  5:340991/138‑1 (MQ=255)
                                         ttttCTGATGCAGATGGTAAATGTCACATCTGTGAGTCAATAATGATTGATAACTCATATTGATCTCATCCGCACTTTCGCTTCTATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATa                                                                                                <  5:193211/138‑1 (MQ=255)
                                         ttttCTCCTGCAGATGGTAAATGTCACATCTGTGAGTCAATAAGGATTGATAACTCATATTGATCTCATCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCa                                                                                                  <  3:229733/136‑1 (MQ=255)
                                         ttttCTCCTGCAGATGGTAAATGTCACATCTGTGAGTCAATAAGGATTGATAACTCATATTGATCTCATCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATa                                                                                                <  5:268317/138‑1 (MQ=255)
                                          tttCTCCTGCAGATGGTAAATGTCACATCTGTGAGTCAATAAGGATTGATAACTCATATTGATCTCATCCGCACTTTCGCTTCAATCCCATCTAAATCATGGTAAGCCGGTTTCACATAATCTTTTATTAATGCATac                                                                                               >  4:395796/1‑138 (MQ=255)
                                            tctcCTGCAGATGGTAAATGTCACATCTGTGAGTCAATAAGGATAGATAACTCATATTGATCTCATCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATAcaca                                                                                            >  7:398154/1‑139 (MQ=255)
                                                cTGCAGATGGTAAATGTCACATCTGTGAGTCAATAAGGATTGATAACTCATATTGATCTCATCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACAt                                                                                           <  3:222498/136‑1 (MQ=255)
                                                        ggTAAATGTCACATCTGTGAGTCAATAAGGATTGATAACTCATATTGATCTCATCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTg                                                                                 >  5:217612/1‑138 (MQ=255)
                                                         gTAAATGTCACATCTGTGAGTCAATAAGGATTGATAACTCATATTGATCTCATCCGCACTTTCGCTTCAATCCCTTCTAGATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGaa                                                                               <  2:321902/139‑1 (MQ=255)
                                                         gTAAATGTCACATCTGTGAGTCAATAAGGATTGATAACTCATATTGATCTCATCCGCACTTTCGCTTCAATCCCTTCTAGATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGaa                                                                               <  6:284394/139‑1 (MQ=255)
                                                         gTAAATGTCACATCTGTGAGTCAATAAGGATTGATAACTCATATTGATCTCATCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGaa                                                                               <  6:217612/139‑1 (MQ=255)
                                                          tAAATGTCACATCTGTGAGTCAATAAGGATTGATAACTCATATTGATCTCATCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTg                                                                                 >  2:226186/1‑136 (MQ=255)
                                                             aTGTCACATCTGTGAGTCAATAAGGATTGATAACTCATATTGATCTCATCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGAagag                                                                            >  3:181774/1‑138 (MQ=255)
                                                                   caTCTGTGAGTCAATTAGGATTGATAACTCATATTGATCTCATCCTCACTTTCGCTTCTATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGAAGAGAACTGcc                                                                     <  1:341677/139‑1 (MQ=255)
                                                                   caTCTGTGAGTCAATAAGGATTGATAACTCATATTGATCTCATCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGAAGAGAACTGcc                                                                     <  4:181774/139‑1 (MQ=255)
                                                                   caTCTGTGAGTCAATAAGGATTGATAACTCATATTGATCTCATCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGAAGAGAACTGcc                                                                     <  5:371898/139‑1 (MQ=255)
                                                                   caTCTGTGAGTCAATAAGGATTGATAACTCATATTGATCTCATCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGAAGAGAACTGcc                                                                     <  1:171976/139‑1 (MQ=255)
                                                                   caTCTGTGAGTCAATAAGGATTGATAACTCATATTGATCTCATCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGAAGAGAACTGcc                                                                     <  3:215750/139‑1 (MQ=255)
                                                                   caTCTGTGAGTCAATAAGGATTGATAACTCATATTGATCTCATCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATATTTTATTAATGCATACACATCAGGTTTTTGAAGTGTACTGcc                                                                     <  7:289939/139‑1 (MQ=255)
                                                                   caTCTGTGAGTCAATAAGGATTGATAACTCATATTGATCTCATCCACACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGAAGAGAACTGcc                                                                     <  1:128607/139‑1 (MQ=255)
                                                                         tgAGTCAATAAGGATTGATAACTCATATTGATCTCATCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGAAGAGAACTGCCATcccc                                                               <  8:90213/139‑1 (MQ=255)
                                                                          gAGTCAATAAGGATTGATAACTCATATTGATCTCATCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGAAGAGAACTGCCATcccc                                                               <  7:333060/138‑1 (MQ=255)
                                                                           aGTCAATAATGATTGATAACTCATATTGATCTCATCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGAAGAGAACTGCCATCCCCTa                                                             <  6:14997/139‑1 (MQ=255)
                                                                           aGTCAATAAGGATTGATAACTCATATTGATCTCATCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGAAGAGAACTGCCATCCCCTa                                                             <  8:325992/139‑1 (MQ=255)
                                                                           aGTCAATAAGGATTGATAACTCATATTGATCTCATCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGAAGAGAACTGCCATCCCCTa                                                             <  8:63377/139‑1 (MQ=255)
                                                                                 tAAGGATTGATAACTCATATTGATCTCATCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATa                                                                                                >  2:356378/1‑98 (MQ=255)
                                                                                 tAAGGATTGATAACTCATATTGATCTCATCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATa                                                                                                <  1:356378/98‑1 (MQ=255)
                                                                                 tAAGGATTGATAACTCATATTGATCTCATCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGAAGAGAACTGCCATCCCCTAGAGta                                                        >  2:24859/1‑138 (MQ=255)
                                                                                          aTAACTCATATTGATCTCATCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATATTTTATTAATGCATACACATCAGGTTTTTGAAGAGAACTGCCATCCCCTAGAGTATAAGAATAGt                                              >  5:273200/1‑139 (MQ=255)
                                                                                               tCATATTGATCTCATCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGAAGAGAACTGCCATCCCCTAGAGTATAAGAATAGTCAcc                                          >  2:566/1‑138 (MQ=255)
                                                                                                cATATTGATCTCATCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATATACATCAGGTTTTTGAAGAGAACTGCCATCCCCTa                                                             >  4:85586/1‑118 (MQ=255)
                                                                                                cATATTGATCTCATCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATATACATCAGGTTTTTGAAGAGAACTGCCATCCCCTa                                                             <  3:85586/118‑1 (MQ=255)
                                                                                                       aTCTCATCCGCACTTTCTCTTCATTGCGTTCTAAATCAGGGTCAGCCGGTTTCACATTTTCTTTTATTAATGGATACACTTCATGTTTTTGATGATAAATGACATCACCTAGTGTATAATAATAGTCACCAAtttttt                                  <  7:128002/138‑1 (MQ=255)
                                                                                                         ctcATCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGAAGAGAACTGCCATCCCCTAGAGTATAAGAATAGTCACCAATTTTTTCAg                               >  5:263065/1‑139 (MQ=255)
                                                                                                         ctcATCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGAAGAGAACTGCCATCCCCTAGAGTATAAGAATAGTCACCAATTTTTTCAg                               >  2:308666/1‑139 (MQ=255)
                                                                                                         ctcATCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGAAGAGAACTGCCATCCCCTAGAGTATAAGAATAGTCACCAATTTTTTCAg                               >  4:180460/1‑139 (MQ=255)
                                                                                                         ctcATCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGAAGAGAACTGCCATCCCCTAGAGTATAAGAATAGTCACCAATTTTTTCAg                               >  6:243213/1‑139 (MQ=255)
                                                                                                           cATCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGAAGAGAACTGCCATcccc                                                               >  1:33207/1‑105 (MQ=255)
                                                                                                           cATCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGAAGAGAACTGCCATcccc                                                               <  2:33207/105‑1 (MQ=255)
                                                                                                            aTCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAGTCTTTTATTAATGCCTTCACATCAGGTTTTTGATGAGAACTGCCATCCACGAGAGTATAAGATTAGTCACCACttttttagtttg                            >  8:361380/1‑133 (MQ=255)
                                                                                                            aTCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGAAGAGAACTGCCATCCCCTag                                                            <  1:204513/107‑1 (MQ=255)
                                                                                                            aTCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGAAGAGAACTGCCATCCCCTag                                                            >  2:204513/1‑107 (MQ=255)
                                                                                                            aTCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGAAGAGAACTGCCATCCCCTa                                                             <  8:139814/106‑1 (MQ=255)
                                                                                                            aTCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGAAGAGAACTGCCATCCCCTATAGTATAAGAATAGTCACCAATTTTTTCAGTTg                            >  2:185356/1‑139 (MQ=255)
                                                                                                            aTCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGAAGAGAACTGCCATCCCCTAGAGTATAAGAATAGTCACCAATTTTTTCa                                <  3:114974/135‑1 (MQ=255)
                                                                                                            aTCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGAAGAGAACTGCCATCCCCTAGAGTATAAGAATAGTCACCAATTTTTTCAGTTg                            >  3:85328/1‑139 (MQ=255)
                                                                                                            aTCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGGTTTTGAAGAGAACTGCCATCCCCTAGAGTATAAGAATAGTCACCAATTTTTTCAg                               <  8:398154/136‑1 (MQ=255)
                                                                                                            aTCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGATTTTGAAGAGAACTGCCATCCCCTa                                                             >  7:139814/1‑106 (MQ=255)
                                                                                                               cGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGAAGAGAACTGCCATCCCCTAGAGTATAAGAATAGTCACCAATTTTTTCAGTTGta                          <  6:263065/138‑1 (MQ=255)
                                                                                                                 cACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGAAGAGAACTGCCATCCCCTa                                                             <  7:155122/101‑1 (MQ=255)
                                                                                                                 cACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGAAGAGAACTGCCATCCCCTa                                                             >  8:155122/1‑101 (MQ=255)
                                                                                                                      tCGCTTCAATCCCTTCTAAATCTGGGTGAGCCGGTATCTCATAATCTTTTATTAATGCATACACATCAGGTTTTTGAAGAGAACTGCCATCCCCTAGAGTATAAGAATAGTCACCAATTTTTTCAGTTGTATATCCtt                   <  7:41691/138‑1 (MQ=255)
                                                                                                                      tCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGAAGAGAACTGCCATCCCCTa                                                             >  7:138702/1‑96 (MQ=255)
                                                                                                                      tCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGAAGAGAACTGCCATCCCCTa                                                             <  8:138702/96‑1 (MQ=255)
                                                                                                                      tCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGAAGAGAACTGCCATCCCCTAGAGTATAAGAATAGTCACCAATTTTTTCAGTTGTATATCCtt                   >  2:337538/1‑138 (MQ=255)
                                                                                                                      tCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGGTTTTGAAGAGAACTGCCATCCCCTAGAGTATAAGAATAGTCACCAATTTTTTCAGTTGTATATCCtt                   <  1:198638/138‑1 (MQ=255)
                                                                                                                       cGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGAAGAGAACTGCCATCCCCTAGAGTATAAGAATAGTCACCAATTTTTTCAGTTGTATATCCttt                  <  1:396765/138‑1 (MQ=255)
                                                                                                                              aTCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGAAGAGAACTGCCATCCCCTa                                                             >  8:289505/1‑88 (MQ=255)
                                                                                                                              aTCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGAAGAGAACTGCCATCCCCTa                                                             <  7:289505/88‑1 (MQ=255)
                                                                                                                                     cTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGAAGAGAACTGCCATCCCCTAGAGTATAAGAATAGTCACCAATTTTTTCAGTTGTATATCCTTTAATAATTGACtcgt    <  3:2757/138‑1 (MQ=255)
                                                                                                                                        aaTCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGAAGAGAACTGCCATCCCCTAGAGTATAAGAATAGTCACCAATTTTTTCAGTTGTATATCCTTTAATAATTGACtcgtcg  <  1:226186/137‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                        
ATCTTCAACCGGCACCCCGAATTTTTGCTTTCTATTTTCTTTTTTCTCCTGCAGATGGTAAATGTCACATCTGTGAGTCAATAAGGATTGATAACTCATATTGATCTCATCCGCACTTTCGCTTCAATCCCTTCTAAATCAGGGTCAGCCGGTTTCACATAATCTTTTATTAATGCATACACATCAGGTTTTTGAAGAGAACTGCCATCCCCTAGAGTATAAGAATAGTCACCAATTTTTTCAGTTGTATATCCTTTAATAATTGACTCGTCG  >  CP006881/2500245‑2500517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑