Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 2,963,937 G→A 67.3% intergenic (‑124/+156) U712_15285 ← / ← U712_15290 Manganese‑binding lipoprotein mntA/o‑succinylbenzoate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,963,9370GA67.3% 52.6 / 47.0 52intergenic (‑124/+156)U712_15285/U712_15290Manganese‑binding lipoprotein mntA/o‑succinylbenzoate synthase
Reads supporting (aligned to +/- strand):  ref base G (15/2);  new base A (30/5);  total (45/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.13e-01

GCCCTTGTCTCATATTTCTCCTCCTCTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCGCGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCATTTA  >  CP006881/2963801‑2964067
                                                                                                                                        |                                                                                                                                  
gCCCGTGTCTCATATTTCTCCTCCTCTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCa                                                                                                                                  <  1:402571/139‑1 (MQ=255)
 cccTTGTCTCATATTTCTCCTCCTCTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGCCATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATACa                                                                                                                                  >  1:328394/1‑138 (MQ=255)
           atatTTCTCCTCCTCTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATACAAAATTATcgcg                                                                                                                       >  3:74276/1‑139 (MQ=255)
           atatTTCTCCTCCTCTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATACAAAATTATcgcg                                                                                                                       >  3:47596/1‑139 (MQ=255)
                 ctcctcctcTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTATTATACATTTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATACAAATTTATCGCGTTTGGc                                                                                                                 >  8:163757/1‑139 (MQ=255)
                    ctcctcTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATACAAAATTATCGCGTTTGGCCAt                                                                                                              <  7:48068/139‑1 (MQ=255)
                            tGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATACAAAATTATCGCGTTTGGCCATTTTTGTc                                                                                                       >  6:289060/1‑138 (MQ=255)
                            tGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATACAAAATTATCGCGTTTGGCCATTTTTGTc                                                                                                       >  6:294642/1‑138 (MQ=255)
                                 tCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATACAAAATTATCTCGTTTGGCCATTTTTGTCAAGCt                                                                                                  >  5:71478/1‑138 (MQ=255)
                                 tCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATACAAAATTATCGCGTTTGGCCATTTTTGTCAAGCt                                                                                                  >  4:238933/1‑138 (MQ=255)
                                 tCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATACAAAATTATCGCGTTTGGCCATTTTTGTCAAGCt                                                                                                  >  5:76417/1‑138 (MQ=255)
                                           aTTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGTAAAATTATCGAGTTTGGCCATTTTTGTCAAGCTGATTTACTTa                                                                                        >  4:65284/1‑138 (MQ=255)
                                               ttttATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATACAAAATTATCGCGTTTGGCCATTTTTGTCAAGCTGATTTACATaaaaa                                                                                    <  7:281216/138‑1 (MQ=255)
                                                    tCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATg                                                                                                                                    >  4:352087/1‑85 (MQ=255)
                                                    tCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATg                                                                                                                                    <  3:352087/85‑1 (MQ=255)
                                                         tgttgtCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATACAAAATTATCGCGTTTGGCCATTTTTGTCAAGCTGATTTa                                                                                            >  3:173961/1‑120 (MQ=255)
                                                         tgttgtCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATACAAAATTATCGCGTTTGGCCATTTTTGTCAAGCTGATTTa                                                                                            <  4:173961/120‑1 (MQ=255)
                                                         tgttgtCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATACAAAATTATCGCGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTggg                                                                         >  1:68447/1‑139 (MQ=255)
                                                         tgttgtCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATACAAAATTATCGCGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTggg                                                                         >  7:224503/1‑139 (MQ=255)
                                                         tgttgtCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATACAAAATTATCGCGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACAGGCTggg                                                                         >  8:230232/1‑139 (MQ=255)
                                                         tgttgtCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATACAAAATAATCGCGTTTGGCCATTTTTGTCAAGATGATTTACATAAAAAAACCGGCTggg                                                                         >  1:367156/1‑139 (MQ=255)
                                                              tCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAGATTATCGCGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTcag                                                                     >  5:40142/1‑138 (MQ=255)
                                                              tCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAGATTATCGCGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGCCTGGGTcag                                                                     >  5:124103/1‑138 (MQ=255)
                                                              tCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATACAAAATTATCGCGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTcag                                                                     >  3:101046/1‑138 (MQ=255)
                                                              tCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATACAAAATTATCGCGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTcag                                                                     >  1:49480/1‑138 (MQ=255)
                                                                       aGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATACAAAATTATCGCGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTc                                                            >  7:322871/1‑138 (MQ=255)
                                                                            tgtgACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGTAAAATTATCGCGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCTGTctctta                                                       >  5:37093/1‑133 (MQ=255)
                                                                            tgtgACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCa                                                                                                                                  >  2:111428/1‑63 (MQ=255)
                                                                            tgtgACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCa                                                                                                                                  <  1:111428/63‑1 (MQ=255)
                                                                            tgtgACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCGCGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAAc                                                       >  4:53626/1‑138 (MQ=255)
                                                                            tgtgACATTAAATTAGCCAAAAGCATGTTTAATTTACACAGGACAAAAAGTTACCCTCATGCAAAATTATCGCGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGACAGAAGCCGGTCCatcc                                                       >  3:70767/1‑135 (MQ=255)
                                                                                 aaTTAATTTACCAAAAAGTATTTTTTATTTACACAGTAAAAAAATTTACAATAATAAAAAATTATCGCGTTTGCCATTTTTTGAAAAGATTATTAAAAAAAAAAACCCGGCTGGGTCAGCAGCCGGTCCAAACGAGaa                                                  <  2:68447/137‑1 (MQ=255)
                                                                                  aTTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAGATTATCGCGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAgg                                                >  2:255653/1‑139 (MQ=255)
                                                                                           gCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCGCGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTAtt                                        >  1:71133/1‑138 (MQ=255)
                                                                                            ccAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATACAAAATTATCGCGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTc                                       >  1:54843/1‑138 (MQ=255)
                                                                                                aaGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATACAAAATTATCGCGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACg                                   >  1:208216/1‑138 (MQ=255)
                                                                                                      gTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACtt                            >  8:83596/1‑139 (MQ=255)
                                                                                                      gTTTTATTTACACAGGACAAAAAGTTTCCCTCATACAAAATTATCGCGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACtt                            >  4:121845/1‑139 (MQ=255)
                                                                                                           atttaCACAGGACAAAAAGTTCCCATCAAACAAATTTTTGGGGGTTGGCTATTTTTTTCATGCTTATTAAAAAAAAAACCCCGGCTGGGTCAGCTGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTAc                        <  2:328394/138‑1 (MQ=255)
                                                                                                              taCACAGGACAAAAAGTTTCCCTCATACAAAATTATCGCGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCACACGAGAAGGGATGTATTCAACGAACACTTTTACTGt                     >  8:249382/1‑138 (MQ=255)
                                                                                                                    ggACAAAAAGTTTCCCTCATACAAAATTATCGCGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGca              >  1:258696/1‑139 (MQ=255)
                                                                                                                     gACAAAAAGTTTCCCTCATACAAAATTATCGCGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCACACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGca              >  7:364175/1‑138 (MQ=255)
                                                                                                                     gACAAAAAGTTTCCCTCATACAAAATTATCGCGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGcac             >  4:71015/1‑139 (MQ=255)
                                                                                                                     gACAAAAAGTTTCCCTCATACAAAATTATCGCGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGca              >  1:190187/1‑138 (MQ=255)
                                                                                                                     gACAAAAAGTTTCCCTCATACAAAATTATCGCGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGca              >  1:363847/1‑138 (MQ=255)
                                                                                                                               ttCCCTCATGTAAAATTATCGCGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCAtt    >  4:309735/1‑138 (MQ=255)
                                                                                                                               ttCCCTCATGCAAGATTATCGCGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCAtt    >  8:270537/1‑138 (MQ=255)
                                                                                                                               ttCCCTCATGCAAAATTATCGCGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCAtt    >  5:46225/1‑138 (MQ=255)
                                                                                                                               ttCCCTCATGCAAAATTATCGCGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCAtt    >  1:42909/1‑138 (MQ=255)
                                                                                                                               ttCCCTCATACAAAATTATCGCGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCAtt    >  3:32822/1‑138 (MQ=255)
                                                                                                                               ttCCCTCATACAAAATTATCGCGTTTGGCCATTTTTGTCAAGCTGATTTAAATAAAAAAACCGGCTGGTTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCAtt    >  3:131757/1‑138 (MQ=255)
                                                                                                                               ttCCCTCATACAAAATTATCGCGTTTGGCCATTTTTGTAAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCGGCCGGTCCAAACGAGAAGGGATGTAGTCAACGAACACTTTTAGTGTAACAGCACAGTTTCAtt    >  5:287327/1‑138 (MQ=255)
                                                                                                                               ttCCCTCATACAAAATTATCGCGTTTGGCCATTTGTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCAtt    >  5:398203/1‑138 (MQ=255)
                                                                                                                                tCCCTCATGCAAAATTATCGCGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCATTTa  >  3:81308/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                  
GCCCTTGTCTCATATTTCTCCTCCTCTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCGCGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCATTTA  >  CP006881/2963801‑2964067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑