Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	363,015	G→T	5.9%	W29L (TGG→TTG)	U712_01715 →	Nitrate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	363,015	0	G	T	5.9%	203.2 / 4.1	68	W29L (TGG→TTG)	U712_01715	Nitrate transporter
Reads supporting (aligned to +/- strand):  ref base G (27/37);  new base T (2/2);  total (29/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.19e-01

ATATCCTTGATTCAGAAAATGTAAAATAATGAGGGTCTAGAAAGGGGAATCTTATGAAGCTGTCGGAACTGAAAACTAGCGGTCATCCACTCACTTTGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGTCTCCTTTTGAGAAAGGGCTTGTCGTAGCTGTGCCGATTTTATCTGGATCTGTGTTTCGTATCATTCTTGGTATTTTAACGGACAGAA  >  CP006881/362877‑363152                                                                                                                                           |                                                                                                                                          atatCCTTGATTCAGAAAATGTAAAATAATGAGGGTCTAGAAAGGGGAATCTTATGAAGCTGTCGGAACTGAAAACTAGCGGTCATCCACTCACTTTGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATg                                                                                                                                           >  1:48068/1‑139 (MQ=255)    tCCTTGATTCAGAAAATGTAAGATAATGAGGGTCTAGAAAGGGGAATCTTATGAAGCTGTCGGAACTGAAAACTAGCGGTCATCCACTCACTTTGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGt                                                                                                                                        <  2:314676/139‑1 (MQ=255)       ttGATTCAGAAAATGTAAAATAATGAGGGTCTAGAAAGGGGAATCTTATGAAGCTGTCGGAACTGAAAACTAGCGGTCATCCACTCACTTTGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTAt                                                                                                                                     >  6:117941/1‑139 (MQ=255)         gATTCAGAAAATGTAAAATAATGAGGGTCTAGAAAGGGGAATCTTATGAAGCTGTCGGAACTGAAAACTAGCGGTCATCCACTCACTTTGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTAt                                                                                                                                     <  6:358277/137‑1 (MQ=255)         gATTCAGAAAATGTAAAATAATGAGGGTCTAGAAAGGGGAATCTTATGAAGCTGTCGGAACTGAAAACTAGCGGTCATCCACTCACTTTGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGc                                                                                                                                   >  4:314931/1‑139 (MQ=255)         gATTCAGAAAATGTAAAATAATGAGGGTCTAGAAAGGGGAATCTTATGAAGCTGTCGGAACTGAAAACTAGCGGTCATCCACTCACTTTGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGc                                                                                                                                   >  3:206546/1‑139 (MQ=255)         gATTCAGAAAATGTAAAATAATGAGGGTCTAGAAAGGGGAATCTTATGAAGCTGTCGGAACTGAAAACTAGCGGTCATCCACTCACTTTGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGc                                                                                                                                   >  1:57107/1‑139 (MQ=255)           ttCAGAAAATCTAAAATAATGAGGGGCTAGAAACGGGAAGCTTATGAAGCTGTCGGAACTGAAAACTAGCGGCCATCCACTCACTTTGCTCTGGTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGCtg                                                                                                                                 <  8:42053/139‑2 (MQ=255)                  aaTCTAAAATACTGAGCTGCTAGAAACGTGAATCGAATGCCTCTGTCGGACCCGAAAACTAGCGGTCATCCACTCACTTTGCTCTGTTGCTTTATATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTTGGgcgc                                                                                                                          <  6:275964/139‑1 (MQ=255)                        aaaTAATGAGGGTCTAGAAAGGGGAATCTTATGAAGCTGTCGGAACTGAAAACTAGCGGTCATCCACTCACTTTGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGgtg                                                                                                                    >  1:156811/1‑139 (MQ=255)                         aataatGAGGGTCTAGAAAGGGGAATCTTATGAAGCTGTCGGAACTGAAAACTAGCGGTCATCCACTCACTTTGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGgtgt                                                                                                                   <  5:113410/139‑1 (MQ=255)                          ataatGAGGGTCTAGAAAGGGGAATCTTATGAAGCTGTCGGAACTGAAAACTAGCGGTCATCCACTCACTTTGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGtt                                                                                                                  >  8:228361/1‑139 (MQ=255)                              tGACGGTATAGTAAGGTGAATCTTATGAAGCTGTCGGAACTGAAAACTAGCGGTCATCCACTCACTTTGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGTTTATGCTTGGGGCGCTGGGTGTTtata                                                                                                              <  4:39824/139‑1 (MQ=255)                                aGGGTCTAGAAAGGGGAATCTTATGAAGCTGTCGGAACTGAAAACTAGCGGTCATCCACTCACTTTGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATAtt                                                                                                            >  7:28942/1‑139 (MQ=255)                                     cTAGAAAGGGGAATCTTATGAAGCTGTCGGAACTGAAAACTAGCGGTCATCCACTCACTTTGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATTGGTTATGCTTGGGGCGCTGGGTGTTTATATTtct                                                                                                         >  3:85390/1‑137 (MQ=255)                                     cTAGAAAGGGGAATCTTATGAAGCTGTCGGAACTGAAAACTAGCGGTCATCCACTCACTTTGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATTGGTTATGCTTGGGGCGCTGGGTGTTTATATTtct                                                                                                         >  3:79665/1‑137 (MQ=255)                                      tAGAAAGGGGAATCTTATGAAGCTGTCGGAACTGAAAACTAGCGGTCATCCACTCACTTTGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAg                                                                                                      >  6:372473/1‑139 (MQ=255)                                          aaGGGGAATCTTATGAAGCTGTCGGAACTGAAAACTAGCGGTCATCCACTCACTTTGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATTGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGAtt                                                                                                  <  4:85390/139‑1 (MQ=255)                                          aaGGGGAATCTTATGAAGCTGTCGGAACTGAAAACTAGCGGTCATCCACTCACTTTGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATTGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGAtt                                                                                                  <  4:79665/139‑1 (MQ=255)                                             gggAATCTTATGAAGCTGTCGGAACTGAAATCTAGCGGTCATCCACTCACTTTGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGtt                                                                                                                                       <  7:145251/99‑1 (MQ=255)                                             gggAATCTTATGAAGCTGTCGGAACTGAAAACTAGCGGTCATCCACTCACTTTGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGtt                                                                                                                                       >  8:145251/1‑99 (MQ=255)                                                aaTCTTATGAAGCTGTCGGAACTGAAAACTAGCGGTCATCCACTCACTTTGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGcc                                                                                            >  7:166516/1‑139 (MQ=255)                                                 aTCTTATGAAGCTGTCGGAACTGAAAACTAGCGGTCATCCACTCACTTTGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCt                                                                                           <  6:294650/139‑1 (MQ=255)                                                      aTGAAGCTGTCGGAACTGAAAACTAGCGGTCATCCACTCACTTTGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGt                                                                                         <  1:123864/136‑1 (MQ=255)                                                       tGAAGCTGTCGGAACTGAAAACTAGCGGTCATCCACTCACTTTGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGTCTcc                                                                                     <  4:206546/139‑1 (MQ=255)                                                              gTCGGAACTGAAAACTAGCGGTCATCCACTCACTTTGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGTCTCCtttt                                                                                 >  1:174628/1‑136 (MQ=255)                                                                cGGAACTGAAAACTAGCGGTCATCCACTCACTTTGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGTCTCCTTTTGAGaa                                                                            <  6:103593/139‑1 (MQ=255)                                                                cGGAACTGAAAACTAGCGGTCATCCACTCACTTTGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCGGGGTGTTTATATTTCTCAGGATTTTGGCCTGTCTCCTTTTGAGaa                                                                            <  4:88488/139‑1 (MQ=255)                                                                 ggAACTGAAAACTAGCGGTCATCCACTCACTTTGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGTCTCCTTTTGAGaa                                                                            <  5:19732/138‑1 (MQ=255)                                                                    aCTGAAAACTAGCGGTCATCCACTCACTTTGCTGTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGTCTCCTTTTGAGAAAggg                                                                        <  6:88328/139‑1 (MQ=255)                                                                    aCTGAAAACTAGCGGTCATCCACTCACTTTGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGTCTCCTTTTGAGAAAggg                                                                        <  8:166516/139‑1 (MQ=255)                                                                    aCTGAAAACTAGCGGTCATCCACTCACTTTGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGTCTCCTTTTGAGAAAggg                                                                        <  8:28942/139‑1 (MQ=255)                                                                        tcaaCTAGCGGGCATCCACTCACTTTGGGCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGTCTCCTTTTGAGAAAGGGCt                                                                      <  8:124361/135‑1 (MQ=255)                                                                        aaaaCTAGCGGTCATCCACTCACTTTGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGTCTCCTTTTGAGAAAGGGCTTg                                                                    >  1:258668/1‑139 (MQ=255)                                                                           aCTAGCGGTCATCCACTCACTTTGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGTCTCCTTTTGAGAAAGGGCTTGTCg                                                                 <  2:156811/139‑1 (MQ=255)                                                                               gCGGTCATCCACTCACTTTGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTCGGTGTTTATTTTTCTAATGATTTTGGCCTGTCTCCTTTAGAGAAAGGGCTTGTCGTAgg                                                             >  1:201721/1‑138 (MQ=255)                                                                                        cactcactTTGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGTCTCCTTTTGAGAAAGGGCTTGTCGTAGCTGTGCCGAt                                                    <  3:314931/139‑1 (MQ=255)                                                                                         actcactTTGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGTCTCCTTTTGAGAAAGGGCTTGTCGTAGCTGTGCCGAtt                                                   <  5:372473/139‑1 (MQ=255)                                                                                             actTTGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGTCTCCTTTTGAGAAAGGGCTTGTCGTAGCTGTGCCGATTTTa                                                >  4:48428/1‑138 (MQ=255)                                                                                             actTTGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGTCTCCTTTTGAGAAAGGGCTTGTCGTAGCTGTGCCGATTTTAt                                               <  2:57107/139‑1 (MQ=255)                                                                                              ctTTGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGGGTTTATATTTCTCAGGATTTTGGCCTGTCTCCTTTTGAGAAAGGGCTTGTCGTAGCTGTGCCGATTTTATc                                              <  6:138087/139‑1 (MQ=255)                                                                                                 tGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGTCTCCTTTTGAGAAAGGGCTTGTCGTAGCtgtg                                                         >  1:190982/1‑125 (MQ=255)                                                                                                 tGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGTCTCCTTTTGAGAAAGGGCTTGTCGTAGCtgtg                                                         <  2:190982/125‑1 (MQ=255)                                                                                                 tGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGTCTCCTTTTGAGAAAGGGCTTGTCGTAGCTGTGCCGATTTTATCTg                                            >  3:236564/1‑138 (MQ=255)                                                                                                 tGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGTCTCCTTTTGAGAAAGGGCTTGTCGTAGCTGTGCCGATTTTATCTg                                            <  2:48068/138‑1 (MQ=255)                                                                                                      tGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGTCTCCTTTTGAGAAAggg                                                                        <  2:384672/105‑1 (MQ=255)                                                                                                      tGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGTCTCCTTTTGAGAAAggg                                                                        >  1:384672/1‑105 (MQ=255)                                                                                                       gTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGTCTCCTTTTGAGAAAggg                                                                        >  3:115476/1‑104 (MQ=255)                                                                                                       gTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGTCTCCTTTTGAGAAAggg                                                                        <  4:115476/104‑1 (MQ=255)                                                                                                       gTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGTCTCCTTTTGAGAAAGGGCTTGTCGTAGCTGTGCCGATTTTATCTGGAt                                         >  5:256252/1‑135 (MQ=255)                                                                                                       gTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGTCTCCTTTTGAGAAAGGGCTTGTCGTAGCTGTGCCGATTTTATCTGGAt                                         <  6:256252/135‑1 (MQ=255)                                                                                                           cTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGTCTCCTTTTGAGAAAGGGCTTGTCGTAGCTGTGCCGATTTTATCTGGATCTGTGtt                                  <  2:174628/138‑1 (MQ=255)                                                                                                           cTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGTCTCCTTTTGAGAAAGGGCTTGTCGTAGCTGTGCCGATTTTATCTGGATCTGTGtt                                  <  1:240747/138‑1 (MQ=255)                                                                                                           cTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGTCTCCTTTTGAGAAAGGGCTTGTCGTAGCTGTGCCGATTTTATCTGGATCTGTGtt                                  <  3:48428/138‑1 (MQ=255)                                                                                                             ttttATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGTCTCCtt                                                                                   >  6:230939/1‑87 (MQ=255)                                                                                                             ttttATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGTCTCCtt                                                                                   <  5:230939/87‑1 (MQ=255)                                                                                                             ttttATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGTCTCCTTTTGAGAAAGGGCTTGTCGTAGCTGTGCCGATTTTATCTGGATCGGTGTTTCg                               >  2:168331/1‑139 (MQ=255)                                                                                                              tttATACTTAGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGATGGCTGTTTATATTTCTCATGATTTTGGCGTCTCTCATTTTGAGAAAGGGCTAGTCGTAGCTGTGCCGATTTTATCTGGATATGTCTTTCg                               >  6:147224/1‑138 (MQ=255)                                                                                                                tataCTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGTCTCCTTTTGAGAAAGGGCTTGTCGTAGCTGTGCCGATTTTATCTGGATCTGTGTTTCGTa                             <  6:112024/138‑1 (MQ=255)                                                                                                                  taCTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGTCTCCTTTTGAGAAAGGGCTTGTCGTAGCTGTGCCGATTTTATCTGGATCTGTGTTTCTTATCa                          >  7:105734/1‑139 (MQ=255)                                                                                                                  taCTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTCGGTGTTTATATTTCTCACGATTTTGGCCTGTCTCCTTTTGAGAAAGGGCTTGTCGTAGCTGTGCCGATTTTATCTGGATCTGTGTATCGTATCa                          >  7:132049/1‑139 (MQ=255)                                                                                                                      ttGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGTCTCCTTTTGAGAAAGGGCTTGTCGTAGCTGTGCCGATTTTATCTGGATCTGTGTTTCGTATCATTCt                      >  2:253102/1‑139 (MQ=255)                                                                                                                       tGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGTCTCCTTTTGAGAAAGGGCTTGTCGTAGCTGTGCCGATTTTATCTGGATCTGTGTTTCGTATCATTCt                      <  7:316097/138‑1 (MQ=255)                                                                                                                        gATGTGAGTTTTATGATATGGGTTATGCTTGGGGAGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGTCTCCTTTTGAGAAAGGGCTTGTCGTAGCTGTGCCGATTTTATCTGGATCTGTGTTTCGTATCATTCtt                     <  2:201721/138‑1 (MQ=255)                                                                                                                               gTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGTCTCCTTTTGAGAAAGGGCTTGTCGTAGCTGTGCCGATTTTATCTGGATCTGTGTTTCGTATCATTCTTGGTAtttt             >  1:396654/1‑139 (MQ=255)                                                                                                                                ttttATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGTCTCCTTTTGAGAAAGGGCTTGTCGTAGCTGTGCCGATTTTATCTGGATCTGTGTTTCGTATCATTCTTGGTATTTTa            <  8:256144/139‑1 (MQ=255)                                                                                                                                     tGATATGGGTTATGCTTGGGGCGCTGGTTGTTTATATTTCTCAGGATTTTGGCCTGTCTCGTTTTGAGAAAGGGCTTGTCGTAGCTGTGCCGATTTTATCTGGATCTGTGTTTCGTATCATTCTTGGTATTTTAACGGa       <  5:117941/139‑1 (MQ=255)                                                                                                                                     tGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGGCTCCTTTTGAGAAAGGGCTTGTCGTAGCTGTGCCGATTTTATCTGGATCTGTGTTTCGTATCATTCTTGGTATTTTAACGGa       <  7:228361/139‑1 (MQ=255)                                                                                                                                          tGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGTCTCCTTTTGAGAAAGGGCTTGTCGTAGCTGTGCAGATTTTATCTGGATCTGTGTTTCGTATCATTCTTGGTATTTTAACGGACAGaa  >  5:398751/1‑139 (MQ=255)                                                                                                                                           |                                                                                                                                          ATATCCTTGATTCAGAAAATGTAAAATAATGAGGGTCTAGAAAGGGGAATCTTATGAAGCTGTCGGAACTGAAAACTAGCGGTCATCCACTCACTTTGCTCTGTTCCTTTTTATACTTTGATGTCAGTTTTATGATATGGGTTATGCTTGGGGCGCTGGGTGTTTATATTTCTCAGGATTTTGGCCTGTCTCCTTTTGAGAAAGGGCTTGTCGTAGCTGTGCCGATTTTATCTGGATCTGTGTTTCGTATCATTCTTGGTATTTTAACGGACAGAA  >  CP006881/362877‑363152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑