Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	2,991,089	C→T	5.4%	noncoding (76/86 nt)	U712_21340 ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,991,089	0	C	T	5.4%	206.0 / 2.4	74	noncoding (76/86 nt)	U712_21340	tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base C (36/34);  new base T (2/2);  total (38/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.70e-01

TACCAAGCTGAGCTACTTCCCGATTTCATATAGATCATGGCGCGCCCGAGAGGAGTCGAACCCCTAACCTTTTGATCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGATGGTATAGTTGGAGCGGAAGACGGGATTCGAACCCG  >  CP006881/2990956‑2991205                                                                                                                                      |                                                                                                                     tACCAAGCTGAGCTACTTCCCGATTTCATATAGCTCATGGCGCGCCCGAGAGGAGTCGAACACCTAACATTTTGATCCGTAGTCAAACGCTCAATCCAATTTAGCTACTGGCGCAACACGTAATGCCGAGGGCCGGCCt                                                                                                                 >  3:247039/1‑139 (MQ=18) tACCAAGCTGAGCTACTTCCCGATTTCATATAGATCATGGCGCGCCCGAGAGGAGTCGAACCCCTAACCTTTTGATCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACt                                                                                                                 >  3:283213/1‑139 (MQ=255)    cAAGCTGAGATACTTCCCGATTTCATATAGATCATGGCGCGCCCTAGATGACTCGAACCCCTAACCTTTTGATACGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAAAGCCGAGGGCCGGACTTGa                                                                                                              <  8:282442/139‑1 (MQ=18)      aGCTTAGCTACTTCCCGAATTCATATAGATGATGGCGCGCCCGAGACGAGTAGAACCCCTATCCTTATGATCCGTAGTCATACGCTCTATCCTATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGTAc                                                                                                            <  4:145023/139‑1 (MQ=17)      aGCTGAGCTACTTCCCGATTTCATATAGATCATGGCGCGCCCGAGAGGAGTCGAACCCCTAACCTTTTGATCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAAc                                                                                                            >  2:180368/1‑139 (MQ=255)        cTGAGCTACTTCCCGATTTCATATAGATCATGGCGCGCCCGAGAGGAGTCGAACCCCTAACCTTTTGATCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGCACCg                                                                                                          >  1:208191/1‑139 (MQ=255)           aGCTACTTCCCGATTTCATATAGATCATGGCGCGCCCGAGAGGAGTCGAACCCCTAACCTTTTGATCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCa                                                                                                       <  6:141470/139‑1 (MQ=255)           aGCTACTTCCCGATTTCATATAGATCATGGCGCGCCCGAGAGGAGTCGAACCCCTAACCTTTTGATCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCa                                                                                                       >  2:322975/1‑139 (MQ=255)           aGCTACTTCCCGATTTCATATAGATCATGGCGCGCCCGAGAGGAGTCGAACCCCTAACCTTTTGATCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCa                                                                                                       <  8:1269/139‑1 (MQ=255)           aGCTACTTCCCGATTTCATATAGATCATGGCGCGCCCGAGAGGAGTCGAACCCCTAACCTTTTGATCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCa                                                                                                       <  3:234627/139‑1 (MQ=255)                 ttCCCGATTTCATATAGATCATGGCGCGCCCGAGAGGAGTCGAACCCCTAACCTTTTGATCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTACTGCCGAGGGCCGGACTTGAACCGGCACGGTAg                                                                                                 >  4:151817/1‑139 (MQ=255)                  tCCCGATTTCATATAGATCATGGCGCGCCCGAGAGGAGTCGAACCCCTAACCTTTTGATCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGTCCGGACTTGAACAGGCACGGTAg                                                                                                 >  7:292916/1‑138 (MQ=255)                  tCCCGATTTCATATAGATCATGGCGCGCCCGAGAGGAGTCGAACCCCTAACCTTTTGATCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGt                                                                                                >  7:299700/1‑139 (MQ=255)                   cccGATTTCATATAGATCATGGCGCGCCCGAGAGGAGTCGAACCCCTAACCTTTTGATCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAg                                                                                                 <  2:241843/137‑1 (MQ=255)                     cGATTTCATATAGATCATGGCGCGCCCGAGAGGAGTCGAACCCCTAACCTTTTGATCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCTGGACTTGAACCGGCACGGTAGTCAc                                                                                             >  8:77019/1‑139 (MQ=255)                     cGATTTCATATAGATCATGGCGCGCCCGAGAGGAGTCGAACCCCTAACCTTTTGATCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCTGGACTTGAACCGGCACGGTAGTCAc                                                                                             >  4:362520/1‑139 (MQ=255)                           cATATAGATCATGGCGCGCCCGAGAGGAGTCGAACCCCTAACCTTTTGATCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATTCAGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCg                                                                                       >  2:371687/1‑139 (MQ=255)                           cATATAGATCATGGCGCGCCCGAGAGGAGTCGAACCCCTAACCTTTTGATCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTCGTCACCTACCg                                                                                       >  2:69661/1‑139 (MQ=255)                             tataGCTCATGGCGCGCCCGAGAGGAGTCGAACCCCTAACCTTTTGATCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCa                                                                                     <  7:182140/139‑1 (MQ=37)                             tataGATCATGGCGCGCCCGAGAGGAGTCGAACCCCTAACCTTTTGATCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCg                                                                                       >  2:41940/1‑137 (MQ=39)                                  aTCAGGGCGCGCCCGAGAGGAGTAGAACAGCTAACCTTTTGATCCGTAGTCAAACGCTCTATCCAAATGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGAt                                                                                 <  1:152123/138‑1 (MQ=11)                                      tGGCGCGCCCGAGAGGAGTCGAACCCCTACCCTTTTGATCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGAtttt                                                                              >  1:185170/1‑137 (MQ=17)                                      tGGCGCGCCCGAGAGGAGTCGAACCCCTAACCTTTTGATCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCa                                                                                                       <  4:63455/112‑1 (MQ=32)                                      tGGCGCGCCCGAGAGGAGTCGAACCCCTAACCTTTTGATCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCa                                                                                                       >  3:63455/1‑112 (MQ=32)                                       ggCGCGCCCGAGAGGAGTCGAACCCCTAACCTTTTGATCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAg                                                                           >  1:51862/1‑139 (MQ=32)                                           cgcCCGAGAGGAGTCGAACCCCTAACCTTTTGATCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCt                                                                       <  3:92189/139‑1 (MQ=32)                                           cgcCCGAGAGGAGTCGAACCCCTAACCTTTTGATCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCt                                                                       >  3:34271/1‑139 (MQ=32)                                             cccGAGAGGAGTCGAACCCCTAACCTTTTGATCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTaa                                                                            >  4:311712/1‑132 (MQ=32)                                             cccGAGAGGAGTCGAACCCCTAACCTTTTGATCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTaa                                                                            <  3:311712/132‑1 (MQ=32)                                                        tCGAACCCCTAACCTTTTGATCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCa                                                                                                       >  8:404199/1‑94 (MQ=33)                                                        tCGAACCCCTAACCTTTTGATCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCa                                                                                                       <  7:404199/94‑1 (MQ=33)                                                        tCGAACCCCTAACCTTTTGATCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCaa                                                          >  3:159126/1‑139 (MQ=32)                                                             cgccTAACCTTTTGATCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGGCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTcca                                                     <  1:371687/137‑1 (MQ=17)                                                             ccccTAACCTTTTGATCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTcca                                                     <  1:69661/139‑1 (MQ=32)                                                                   aCCTTTTGATCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCTGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCaccacc                                                <  3:362520/138‑1 (MQ=17)                                                                   aCCTTTTGATCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCTGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCaccacc                                                <  7:77019/138‑1 (MQ=17)                                                                   aCCTTTTGATCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCaccacc                                                <  1:41940/138‑1 (MQ=32)                                                                        ttGATCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGc                                           <  5:39859/138‑1 (MQ=32)                                                                        ttGATCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCg                                          >  2:50303/1‑139 (MQ=32)                                                                        ttGATCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCg                                          >  5:347755/1‑139 (MQ=32)                                                                        ttGATCCGTAGTCAAACGCTCTATCCAATAGAGCTACGGGCGCAAAACTTAATGCCGCGCGCCGGACTTGAACCCGCACGCTAGTCACCTACCGCAGTATTTTAAGTCCTGTGAGTCTGCCAAATCCACCACCCCGGCg                                          >  2:155855/1‑139 (MQ=11)                                                                           aTCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTg                                        <  1:180368/138‑1 (MQ=32)                                                                            tcagTAGTCATACGCTATATACAATTGAGCTACTGGCGCAAAACTTAATTCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGa                                      <  8:292916/136‑1 (MQ=11)                                                                            tCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACTGTAGGCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGa                                      <  6:67301/139‑1 (MQ=17)                                                                            tCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGa                                      <  8:240851/139‑1 (MQ=32)                                                                            tCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGa                                      <  8:299700/139‑1 (MQ=32)                                                                            tCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGa                                      <  8:83624/139‑1 (MQ=32)                                                                            tCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGa                                      <  4:283213/139‑1 (MQ=32)                                                                            tCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGa                                      <  4:247039/139‑1 (MQ=32)                                                                              cgcacccAACCGCTCTAACCAATTCCCCTACGGGCGCAAAACTAAATGCCGAGGGCCGGACTTGACCCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGGCTGCCAATTCCACCACCCCGGCGTGGa                                      <  8:316205/131‑1 (MQ=11)                                                                               gTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGa                                      >  1:2956/1‑136 (MQ=32)                                                                               gTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGa                                      <  2:2956/136‑1 (MQ=32)                                                                               gTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGACGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGGACGATTTTAAGTCCTGTGTGGCTGCCAATTACACCACCCCGGCGTggcggt                                   >  2:391787/1‑135 (MQ=11)                                                                                         gCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGGTGGGATAGTTg                           >  3:383824/1‑137 (MQ=17)                                                                                             tATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGTGGGTATAGTTGGGGCgg                     >  6:88886/1‑139 (MQ=12)                                                                                             tATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGATGGGATAGTTGGAGCgg                     >  7:36295/1‑139 (MQ=17)                                                                                                    ttGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGc                                           >  4:198658/1‑110 (MQ=32)                                                                                                    ttGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGc                                           >  4:191957/1‑110 (MQ=32)                                                                                                    ttGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGc                                           <  3:191957/110‑1 (MQ=32)                                                                                                    ttGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGc                                           <  3:198658/110‑1 (MQ=32)                                                                                                    ttGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGt                                         >  1:295633/1‑112 (MQ=32)                                                                                                    ttGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGt                                         <  2:295633/112‑1 (MQ=32)                                                                                                    ttGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGCTGGTATTGTTGGAGCGGCAGACgg              >  5:202254/1‑139 (MQ=12)                                                                                                    ttGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGATGGTATAGTTGGAGCGGAAGACgg              >  4:168104/1‑139 (MQ=32)                                                                                                       aGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGATGGTATAGTTGGGGCGGAAGACGGGAt           >  7:290555/1‑139 (MQ=17)                                                                                                         cTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGa                                      >  3:244889/1‑110 (MQ=32)                                                                                                         cTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGa                                      <  4:244889/110‑1 (MQ=32)                                                                                                         cTACGGGCGCAAAACTTAATGCCGAGCGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGa                                      <  8:160451/110‑1 (MQ=17)                                                                                                         cTACGGGCGCAAAACTTAATGCCGAGCGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGa                                      >  7:160451/1‑110 (MQ=17)                                                                                                            cGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGATGGGATATTTTGTGCGGAAGACGGGTTTCgga      >  7:296284/1‑137 (MQ=11)                                                                                                             gggCGAAAAACTTATTGCCGTGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGTTTTTAAGTCCGGTGTGTCTGCCAATTCCACCACCCCGGCGTGGATGGTATAGTTGGCGCGGAAGACGGGATTCGAAc     <  8:290555/139‑1 (MQ=11)                                                                                                                cgaaaaaCTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGACCTGTGTGTCTGCCAATTCCACCCCCCCGGCGTGGATGGTATAGTTGGAGCGGAAGACGGGATTCGAACCcg  <  3:187969/136‑1 (MQ=12)                                                                                                                  cAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCAc                                                                                             >  5:91304/1‑46 (MQ=38)                                                                                                                  cAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCAc                                                                                             <  6:91304/46‑1 (MQ=38)                                                                                                                                      |                                                                                                                     TACCAAGCTGAGCTACTTCCCGATTTCATATAGATCATGGCGCGCCCGAGAGGAGTCGAACCCCTAACCTTTTGATCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGATGGTATAGTTGGAGCGGAAGACGGGATTCGAACCCG  >  CP006881/2990956‑2991205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑