BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	3,699,895	C→T	6.0%	R35R (CGG→CGA)	U712_19055 ←	Hypothetical Protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,699,895	0	C	T	6.0%	265.6 / 3.1	83	R35R (CGG→CGA)	U712_19055	Hypothetical Protein
Reads supporting (aligned to +/- strand): ref base C (33/45); new base T (3/2); total (36/47)
Fisher's exact test for biased strand distribution p-value = 6.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.45e-01

ATATCTCCCGGATAACCCAGCTTCCGAAGGGCTTCCTTAATGATTGTTTGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGATTCACCCTTATCATCATGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTA  >  CP006881/3699757‑3700033

atatCTCCCGGATAACCCAGCTTCCGAAGGGCTTCCTTAATGATTGTTTGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGc                                                                                                                                            <  4:5812/139‑1 (MQ=255)

atatCTCCCGGATAACCCAGCTTCCGAAGGGCTTCCTTAATGATTGTTTGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGc                                                                                                                                            <  8:38804/139‑1 (MQ=255)

atatCTCCCGGATAACCCAGCTTCCGAAGGGCTTCCTTAATGATTGTTTGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGc                                                                                                                                            <  1:364171/139‑1 (MQ=255)

  atCTCCCGGATAACCCAGCTTCCGAAGGGCTTCCTTATTGATTGTTTGGGTAGCCAGAAAGAAGTTAAGAGCTTCTTTTTCTTCGTCTTTCGAGACTTGCTTTGCTGACATGTTTGTCACCGCATTGGTTTTTTGGTCg                                                                                                                                          >  1:186090/1‑139 (MQ=255)

  atCTCCCGGATAACCCAGCTTCCGAAGGGCTTCCTTAATGATTGTTTGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCg                                                                                                                                          >  7:357115/1‑139 (MQ=255)

  atCTCCCGGATAACCCAGCTTCCGAAGGGCTTCCTTAATGATTGTTTGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCg                                                                                                                                          >  2:59988/1‑139 (MQ=255)

  atCTCCCGGATAACCCAGCTTCCGAAGGGCTTCCTTAATGATTGTTTGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCg                                                                                                                                          >  8:227275/1‑139 (MQ=255)

   tctcCCGGATAACCCAGCTTCCGAAGGGCTTCCTTAATGATTGTTTGGGTAGCCAGAAAGAAGTTAAGCGCTTCTTTTTCTTCAGCTTTCGAGACTTGCTTTGCTGACATCTTTTTCGCCTCATTGTTTTGTTGGCCgg                                                                                                                                         >  8:352038/1‑139 (MQ=255)

   tctcCCGGATAACCCAGCTTCCGAAGGGCTTCCTTAATGATTGTTTGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCgg                                                                                                                                         >  3:9591/1‑139 (MQ=255)

   tctcCCGGATAACCCAGCTTCCGAAGGGCTTCCTTAATGATTGTTTGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCgg                                                                                                                                         >  4:202608/1‑139 (MQ=255)

   tctcCCGGATAACCCAGCTTCCGAAGGGCTTCCTTAATGATTGTTTGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCgg                                                                                                                                         >  2:81374/1‑139 (MQ=255)

   tctcCCGGATAACCCAGCTTCCGAAGGGCTTCCTTAATGATTGTTTGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCgg                                                                                                                                         >  8:213131/1‑139 (MQ=255)

   tctcCCGGATAACCCAGCTTCCGAAGGGCTTCCTTAATGATTGTTTGGGTAGACAGAAATAAGTTAAGAGCTTCTTTATCTTCATCTTTCTAGACTTGCTTTGCTGACATCTATTTCACCTAAATGATTTTTTGGCCgg                                                                                                                                         >  8:157283/1‑139 (MQ=255)

       ccGGATAACCCAGCTTCCGAAGGGCTTCCTTAATGATTGTTTGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGTCGGCtgt                                                                                                                                     >  6:303065/1‑139 (MQ=255)

       ccGGATAACCCAGCTTCCGAAGGGCTTCCTTAATGATTGTTTGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGc                                                                                                                                        <  4:9591/136‑1 (MQ=255)

          gATAACCCAGCTTCCGAAGGGCTTCCTTAATGATTGTTTGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACATCATTGTTTTTTTGGCCGGCtgtaat                                                                                                                                  >  8:118315/1‑139 (MQ=255)

              aCCCAGCTTCCGAAGGGCTTCCTTAATGATTGTTTGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGc                                                                                                                                            >  8:407313/1‑125 (MQ=255)

              aCCCAGCTTCCGAAGGGCTTCCTTAATGATTGTTTGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGc                                                                                                                                            <  7:407313/125‑1 (MQ=255)

               cccAGCTTCCGAAGGGCTTCCTTAATGATTGTTTGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCAGCTCATTGTTTTTTTGGCCGGCTGTAATGTAAg                                                                                                                             >  7:113397/1‑139 (MQ=255)

                  aGCTTCCGAAGGGCTTCCTTAATGATTGTTTGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAgcgc                                                                                                                          <  8:153807/139‑1 (MQ=255)

                  aGCTTCAGTAGTGCTTCCTTAATGATTGTTTGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGAGTTGCTTTGCTTACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCGGTAATGTAAgcgc                                                                                                                          <  3:381274/139‑1 (MQ=255)

                        cGAAGGGCTTCCTTAATGATTGTTTGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTaa                                                                                                                     <  2:87267/138‑1 (MQ=255)

                          aaGGGCTTCCTTAATGATTGTTTGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTaaaa                                                                                                                   >  6:329754/1‑138 (MQ=255)

                          aaGGGCTTCCTTAATGATTGTTTGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTaaaa                                                                                                                   >  3:202131/1‑138 (MQ=255)

                          aaGGGCTTCCTTAATGATTGTTTGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTaaaa                                                                                                                   >  4:155467/1‑138 (MQ=255)

                               cTTCCTTAATGATTGTTTGGGTAGACAGAACTAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAg                                                                                                             <  8:113397/139‑1 (MQ=255)

                               cTTCCTTAATGATTGTTTGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAg                                                                                                             <  5:9315/139‑1 (MQ=255)

                               cTTCCTTAATGATTGTTTGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAg                                                                                                             <  1:275858/139‑1 (MQ=255)

                               cTTCCTTAATGATTGTTTGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAg                                                                                                             >  2:174476/1‑139 (MQ=255)

                               cTTCCTTAATGATTGTTTGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAg                                                                                                             >  2:130184/1‑139 (MQ=255)

                               cTTCCTTAATGATTGTTTGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAg                                                                                                             <  1:84285/139‑1 (MQ=255)

                               cTTCCTTAATGATTGTTTGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTATCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAg                                                                                                             <  4:23437/139‑1 (MQ=255)

                                  ccTTAATGATTGTTTGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGccc                                                                                                          >  2:172097/1‑139 (MQ=255)

                                  ccTTAATGATTGTTTGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGc                                                                                                            <  1:108255/137‑1 (MQ=255)

                                  ccTTAATGATTGTTTGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCGCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGccc                                                                                                          >  4:123240/1‑139 (MQ=255)

                                  ccTTAATGATTGATAGGGTAGACAGAAATAAGTTAAGAGCTGCTTTTTCTTCATCTTTCGAGACTTGCTTTGATGACATCTTTTTACCCTCATTGTTTTTTAGGCCGGCTGTAATGAAAGCGCTTTAAAAAATCAGacc                                                                                                          >  8:139477/1‑139 (MQ=255)

                                       aTGATTGTTTGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCaa                                                                                                     >  8:303657/1‑139 (MQ=255)

                                            tGTTTGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCt                                                                                                >  8:324701/1‑139 (MQ=255)

                                            tGTTTGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCt                                                                                                >  8:44959/1‑139 (MQ=255)

                                            tGTTTGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCt                                                                                                >  7:24587/1‑139 (MQ=255)

                                            tGTTTGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCt                                                                                                >  5:205709/1‑139 (MQ=255)

                                            tGTTTGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACATCATTGTTTTTTTGGTCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCt                                                                                                >  1:183811/1‑139 (MQ=255)

                                            tGTTTGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATATTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCTCCaaggcgt                                                                                                >  8:350705/1‑134 (MQ=255)

                                              tttGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGc                                                                                                                                            >  5:114450/1‑93 (MQ=255)

                                              tttGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGc                                                                                                                                            >  8:267073/1‑93 (MQ=255)

                                              tttGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGc                                                                                                                                            <  6:114450/93‑1 (MQ=255)

                                              tttGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGc                                                                                                                                            <  7:267073/93‑1 (MQ=255)

                                              tttGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCttt                                                                                              <  1:172097/139‑1 (MQ=255)

                                              tttGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCttt                                                                                              <  1:174476/139‑1 (MQ=255)

                                               ttGGGTAGAGAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCttt                                                                                              <  5:291038/138‑1 (MQ=255)

                                                tGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGc                                                                                                                                            <  6:78841/91‑1 (MQ=255)

                                                tGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGc                                                                                                                                            >  5:78841/1‑91 (MQ=255)

                                                   gTAGTCAGTAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATATTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCa                                                                                         <  7:157283/139‑1 (MQ=255)

                                                   gTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCa                                                                                         <  8:357115/139‑1 (MQ=255)

                                                    tAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCAt                                                                                        <  2:383018/139‑1 (MQ=255)

                                                    gaGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCAt                                                                                        <  5:386417/138‑1 (MQ=255)

                                                          gAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGTCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGAtt                                                                                  <  5:303065/139‑1 (MQ=255)

                                                          gAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGAtt                                                                                  <  7:213131/139‑1 (MQ=255)

                                                          gAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGAtt                                                                                  <  4:202131/139‑1 (MQ=255)

                                                          gAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGAtt                                                                                  <  1:226443/139‑1 (MQ=255)

                                                          gAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGAtt                                                                                  <  5:107184/139‑1 (MQ=255)

                                                          gAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGAtt                                                                                  <  2:82855/139‑1 (MQ=255)

                                                          gAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGAtt                                                                                  <  5:408544/139‑1 (MQ=255)

                                                          gAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGAtt                                                                                  <  4:62397/139‑1 (MQ=255)

                                                          gAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGAtt                                                                                  <  4:83413/139‑1 (MQ=255)

                                                                 gTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGc                                                                                                                                            <  1:328714/74‑1 (MQ=255)

                                                                 gTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGc                                                                                                                                            >  2:328714/1‑74 (MQ=255)

                                                                     agagCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGATTCACCCTTatca                                                                       >  1:18923/1‑139 (MQ=255)

                                                                     agagCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGATTCACCCTTatc                                                                        >  5:255547/1‑138 (MQ=255)

                                                                      gagCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGATTCACCCTTatcat                                                                      <  1:81374/139‑1 (MQ=255)

                                                                      gagCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGATTCACCCTTatcat                                                                      <  8:183898/139‑1 (MQ=255)

                                                                        gCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGATTCACCCTTAtcatca                                                                    <  1:59988/139‑1 (MQ=255)

                                                                           tcttTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGc                                                                                                                                            <  5:323494/64‑1 (MQ=255)

                                                                           tcttTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGc                                                                                                                                            >  6:323494/1‑64 (MQ=255)

                                                                            cttTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGATTCACCCTTATCATCATGc                                                                 >  3:362963/1‑138 (MQ=255)

                                                                            cttTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGATTCACCCTTATCATCATGc                                                                 <  6:205709/138‑1 (MQ=255)

                                                                            cttTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCAATGGATTCACCCTTATCATCATGc                                                                 <  7:227275/138‑1 (MQ=255)

                                                                            cttTTTCTTCATCGTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGTCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGATTCACCCTTATCATCATGc                                                                 <  2:183811/138‑1 (MQ=255)

                                                                                   ttcATCTTTCGAGACGTGCTTTGCTGCCATATTTTTCACCTCCTTGTTTTTTTTGCCGGCTGTAATGTAAGCGCTTTAAAAAATAAGCCCGCCAATTACTTTCTTAATTGGTGTCACCCTTATCATCATGCAAGATCCg                                                         >  7:306906/1‑139 (MQ=255)

                                                                                      aTCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGATTCACCCTTATCATCATGCAAGATCCGTAc                                                      <  1:130184/139‑1 (MQ=255)

                                                                                           tCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGATTCACCCTTATCATCATGCAAGATCCGTAcc                                                     <  2:3442/135‑1 (MQ=255)

                                                                                           tCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGATTCACCCTTATCATCATGCAAGATCCGTAcc                                                     <  5:329754/135‑1 (MQ=255)

                                                                                            cGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGATTCACCCTTATCATCATGCAAGATCCGTACCAGCtt                                                >  7:327079/1‑139 (MQ=255)

                                                                                                        tgctGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGATTCACCCTTATCATCATGCAAGATCCGTACCAGCTTTTGAATCcaca                                     <  1:133707/138‑1 (MQ=255)

                                                                                                             aCATATTTTTCACGTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTTGGTTCACCCTTATCATCATGCAAGATCCGTACCATCTTTTGAATCCACaaaaaa                                <  8:306906/138‑1 (MQ=255)

                                                                                                                    tttCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGATTCACCCTTATCATCATGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACt                         <  6:255547/138‑1 (MQ=255)

                                                                                                                       cACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGATTCACCCTTATCATCATGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGaa                      <  3:202608/138‑1 (MQ=255)

                                                                                                                       cACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGATTCACCCTTATCATCATGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGaa                      <  7:324701/138‑1 (MQ=255)

                                                                                                                        aCCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGATTCACCCTTATCATCATTCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGaaa                     <  4:362963/138‑1 (MQ=255)

                                                                                                                        aCCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGATTCACCCTTATCATCATGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGaaa                     <  7:118315/138‑1 (MQ=255)

                                                                                                                            cATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCTTTGGATTCACCCTTATCATCATGCAAGATCCGTCCCAGCTTTTTAATCCACAAAAAAAGAGACTGGTAACaag                >  7:397243/1‑137 (MQ=255)

                                                                                                                            cATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGATTCACCCTTATCATCATGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACAtc                >  8:172194/1‑139 (MQ=255)

                                                                                                                            cATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGATTCACCCTTATCATCATGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACAt                 >  8:167030/1‑138 (MQ=255)

                                                                                                                             aTTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGATTCACCCTTATCATCATGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACAtct               <  5:177614/139‑1 (MQ=255)

                                                                                                                             aTTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGATTCACCCTTATCATCATGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACAtct               <  7:303657/139‑1 (MQ=255)

                                                                                                                                      ttGGCCGTCTGTAATGTAAGCGCTTTAAAAAATCAGACCGCCAATTCCTTTCTTCATTGGATTCACCCTTATCATCATGCAAGATACGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTctct       <  7:350705/138‑1 (MQ=255)

                                                                                                                                      ttGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGATTCACCCTTATCATCATGCAAGATCCGTCCCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTctct       <  7:44959/138‑1 (MQ=255)

                                                                                                                                         gCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGATTCACCCTTATCATCATGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGAc    <  7:167030/138‑1 (MQ=255)

                                                                                                                                          ccGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGATTCACCCTTATCATCATGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTa  <  3:155467/139‑1 (MQ=255)

ATATCTCCCGGATAACCCAGCTTCCGAAGGGCTTCCTTAATGATTGTTTGGGTAGACAGAAATAAGTTAAGAGCTTCTTTTTCTTCATCTTTCGAGACTTGCTTTGCTGACATCTTTTTCACCTCATTGTTTTTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGATTCACCCTTATCATCATGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTA  >  CP006881/3699757‑3700033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`