| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 3,713,242 | C→A | 16.6% | L131F (TTG→TTT) | U712_19130 ← | Putative purine permease ywdJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 3,713,242 | 0 | C | A | 16.6% | 147.2 / 3.1 | 63 | L131F (TTG→TTT) | U712_19130 | Putative purine permease ywdJ |
| Reads supporting (aligned to +/- strand): ref base C (40/11); new base A (2/10); total (42/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.19e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCAGCGATCACAACAAGCGCCAGGCCAAATACAAGACCGTCCACCCCGTCCTGTCGATAACCGATTCCGAGAATTCCTTTTATAATGGGCTGACTCAGCTGCATGACAAGAAGCAATAAATAGACGCCCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATGACCTTGAAAACACTTAACAAAAAGAAACATACAGCGCTTACAAGAAGAGCTCCCTGCAAACCCCGCAGTGTGTCTCCATATGT > CP006881/3713129‑3713379 | gCAGCGATCACAACAAGCGCCAGGCCAAATACAAGACCGTCCACCCCGTCCTGTCGATAACCGATTCCGAGAATTCCTTTTATAATGGGCTGACTCAGCTGCATGACAAGAAGCAATAAATAGACGCCCGTTACAACCg > 5:77933/1‑139 (MQ=255) acaacaAGCGCCAGGCCAAATACAAGACCGTCCACCCCGTCCTGTCGATAACCGATTCCGAGAATTCCTTTTATAATGGGCTGACTCCGCTGCATGACAAGAAGCAATAAATAGACGCCCGTTACAACCGGAGTAAAc > 8:175401/1‑138 (MQ=255) caacaaGCCCCAGGCCAAAAACAAGCCCGTCCACCCCGTCCTGTCTATAACCGATTCCTAGAATTCATTTTATATTGGGCTGACTCAGCTGCATCAAAATAACAAAAAAATCGACGCCCGTTACAACCGGAGTAAACaa < 2:288997/139‑1 (MQ=255) gcgcCAGGCCAAATACAAGACCGTCCACCCCGTCCTGTCGATAACCGATTCCGAGAATTCCTTTTATAATGGGCTGACTCCGCTGCATGAAAAGAAGCAATAAATAGACGCCCGTTACAACCGGAGTAAACAATTTTg > 8:54751/1‑138 (MQ=255) gcgcCAGGCCAAAAACAAGACCGTCCACCCCGTCCTGTCGATACCCGATTACGAGAATTGCTTATATAATGGTCTTACTCAGCTGCCTGACAAGAACAAATAAATAGACGCCCGTTGAAACCGGAGTAAACAAttttt > 6:216194/1‑137 (MQ=255) gcgcCAGGCAAAATACAAGACCGTCGACCCCGTCCTGTCGATAACCGATTCCGAGAATTCCTTTTATAATGGGCTGACTCAGCAGCATGACAAGAAGCAATAAATACACGCACTTTACAAACGGAGTAAAAAATTTTg > 4:389279/1‑138 (MQ=255) cAGGCAAATAACAACACCGTCCACCCGGTCCTGTCTATAACCGATTCCTAGAATTCATTTTATAATGGGCTGACTCAGCTGAATCACAAGAAGAAATAAATAGACGCCCGTTACAACCGGAGTAAACAATTTTGCAAGt < 7:227912/139‑1 (MQ=255) aGGCCAAATACAAGACCGTCCACCCCGTCCTGTCGATAACCGATTCCGAGAATTCCTTTTATAATGGGCTGACTCAGCTGCATGACAAGAAGCAATAAATAGACGCCCGTTAAAACCGGAGTAAACAATTTTGCAAGTc > 4:259956/1‑139 (MQ=255) aGGCCAAATACAAGACCGTCCACCCCGTCCTGTCGATAACCGATTCCGAGAATTCCTGTTATAATGGGCTGACTCAGCTGCATGACAAGAAGCAATAAATAGCCGCCCGTTACAACCGGAGTAAACAATTTTGCAAGTc > 8:381899/1‑139 (MQ=255) aGGCCAAATACAAGACCGTCCACCCACTCATATATAAAAAAAAATCCGAGAAATCCTTTTATAATGGGCTGACTCAGCTGCATGACAAGAAGCAATAAATAGAAGCCCGTTACAACCTGAGTAAACAATTTTGCAAGTc > 6:125888/1‑139 (MQ=255) tccAAGACCGCCCACCCCGACCTGATTATAACGTTTACCGATAATTACTTTTAAAATGGGCTGACTCAGCTGCATTAAAACAAGAAAAAAATACACGCCCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATg < 1:380632/137‑1 (MQ=255) cAACACCGCCCACCCGGTCCGGTCGTTACCCGTTTCCTATAATTCTTTTTATAAGGGCCTCACCCTGGTGCATCACAAGACCCAAAAAATCCACGCCCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATGAc < 4:98680/139‑1 (MQ=255) aGACCGTCCACCCCGTCCTGTCGATAACCGATTCCGAGAATTACTTTTATAATGGGCTGACTCAGCTGCATGACAAGAAGCAATAAATAGACGCCCGTTACAACCGGAGTAAACAATTTTGAAAGTCTGTCAATGACCt > 1:38479/1‑139 (MQ=255) tCCACCCCGTCCTGTCGATAACCGATTCCGAGAATTCCTTTTATAATTGGCTGACTCCGCTGCATGACAAGAAGCAATAAATAGACGCCCGTTACAACCGGAGTAAAAAATTTTGCAAGTCTGTCAATGACCTTGaaa > 3:42785/1‑138 (MQ=255) tCCACCCCGTCCTGTCGATAACCGATTCCGAGAATTCCTTTTATAATGGGCTGACTCAGCTGCATGACAAGAAGCAATAAATAGACGCCCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATGACCTTGaaaa > 1:181365/1‑139 (MQ=255) tCCACCCCGTCCTGTCGATAACCGATTCCGAGAATTCCTTTTATAATGGGCTGACTCAGCTGCATGACAAGAAGCAATAAATAGACGCCCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATGACCTTGaaaa > 6:355868/1‑139 (MQ=255) tCCACCCCGTCCTGTCGATAACCGATTCCGAGAATTCCTTTTATAATGGGCTGACTCAGCTGCATGACAAGAAGCAATAAATAGACGCCCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATGACCTTGaaaa > 3:98680/1‑139 (MQ=255) tCCACCCCGTCCTGTCGATAACCGATTCCGAGAATTCCTTTTATAATGGGCTGACTCAGCTGCATGACAAGAAGCAATAAATAGACGCCCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATGACCTTGaaaa > 3:28155/1‑139 (MQ=255) tCCACCCCGTCCTGTCGATAACCGATTCCGAGAATTCCTTTTATAATGGGCTGAATCAGCAGCATGACAAGAAGCAATAAATAGACGCCCGTTACAACCGGAGTAAAAAATTTTGCAAGTCTGTCAATGACCtgtctgt > 3:276650/1‑133 (MQ=255) tCCACCCCGTCCTGTCGATAACCGATTCCGAGAATTCCTGTTATAATGGGCTGACTCAGCTGCATGACAAGAAGCAATAAATAGACCCCCGTTACAACCGGTGTAAACAAATTTGCAAGTCTGTCAATGACCTTGaca > 6:241423/1‑136 (MQ=255) tCCACCCCGTCCTGTCGATAACCGATTACGAGAATTCCGTTTATAATGGGCTTACTAATATTCATGACAAGAAGCAAAAAATAGACGCCCGGAACAACCGGAGTAAACAATTTTGAAAGTATTTCAATGACAGTGaaa > 6:274289/1‑138 (MQ=255) cccGTCCTGTCGATAACCGATTCCGAGAATTCCGTTTATAATGGGCTTACTCAGCTGCATGCCAATCAGCAATAAAAAGACGCCCGGTACACCCGGAGTAAACAATTTTGCAAGTCTGTCAATGACCTGGAAAACACt > 6:227177/1‑138 (MQ=255) gTCCTGTCGATACCCGATTCCGAGAATTCCTTTTATAATGGGCTGACTAAGCTGCATGACAAGAAGCAAAAAATAGACGCCCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATGACCTTGAAAACACGTaa > 1:37788/1‑138 (MQ=255) tCCTGTCGATAACCGATTCCGAGAATTCCTTTTATAATGGGCTGACTCAGCTGCATGACAAGAAGCAATAAATAGACGCCCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATGACCTTGAAAACACTTAAc > 5:156457/1‑138 (MQ=255) tCCTGTCGATAACCGATTCCGAGAATTCCTTTTATAATGGGCTGACTCAGCTGCATGACAAGAAGCAATAAATAGACGCCCGTTACAACCGGAGTAAACAATTTTGCAAGGCTGTCAATGACCTTGAAAACACTTAAc > 8:387991/1‑138 (MQ=255) tCCTGTCGATAACCGATTCCGAGAATTCCTTTTATAATGGGCTGACGCAGCTGCAAGACAAGAAGCAATAAATAGACGCCCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATGACCTGGAACACACTTAAc > 1:338091/1‑138 (MQ=255) gccTGTCGAAACCGGTTTCCGAGAATTCCTTTTATATTGGGCTGACTAAGCTGCATGACAAGAAGAAATAAATAGACGCCCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATGACCTTGAAAACACTTAACa < 7:101990/138‑1 (MQ=255) ccTGTCGATAACCGATTCCGAGAATTCCTTTTATAATGGGCTGACTCAGCTGCATGACAAGAAGCAATAAATAGACg > 1:19013/1‑77 (MQ=255) ccTGTCGATAACCGATTCCGAGAATTCCTTTTATAATGGGCTGACTCAGCTGCATGACAAGAAGCAATAAATAGACg < 2:19013/77‑1 (MQ=255) ccTGTCGATAACCGATTCCGAGAATTCCTTTTATAATGGGCTGACTCAGCTGCATGACAAGAAGCAATAAATAGACGCCCGTTACAACCCGAGTAAACAATTTTGCAAGTCTGTCAATGACCTTGAAAACACTTAACa > 6:347808/1‑138 (MQ=255) gTCCATAACCGATTCCGACAAAACCTTTTAAAAGGGGCGGACTCAGCTGCATGACAAGACGCAAAAAATAGACGCCCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATGACCTTGAAAACACTTAACaaaaa < 8:16277/139‑1 (MQ=255) gaaaaCCGATTCAGAAAATTCATTTTATAATGGGGGGACTCAGCTCCATCAAAAGAACAAATAAATCGACGCCCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATGACCTTGAAAACACTTAACAAAAAGaa < 3:158522/136‑1 (MQ=255) aaCCGATTCCGAGAATTCCTTTTATAATGGGCTGACTCAGCTGCATGACAAGAAGCAATAAATAGACGCCCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATGACCTTGAAAACACTTAACAAAAAGAAAc > 1:368858/1‑138 (MQ=255) ccGATACCCATAATTCATTTTATATGGGGCCGACGCTGCTGCATCACAAGAATAAATAAATCCACGCCCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATGACCTTGAAAACACTTAACAAAAAGAAACATa < 2:181365/139‑1 (MQ=255) aTTCCGAGAATTCCTTTTATAATGGGCTGACTCAGCTGCATGACAAGAAGCAATAAATAGACGCCCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATGACCTTGAAAACACTTAACAAAAAGAAACATACAg > 6:105255/1‑139 (MQ=255) aaTTCATTTTATAAGGGGCTGACTCATCTGAATTAAAAGAATAATAAAATCGACGCCCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATGACCTTGAAAACACTTAACAAAAAGAAACATACAGCGCTTAc < 3:68601/138‑1 (MQ=255) tCCTTTTATAATGGGCTGACTCAGCTGCATGACAAGAAGCAATAAATAGCCGCCCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATGACCTTGAAAACACTTAACAAAAAGAAACATACAGCGCTTACaag > 8:400349/1‑138 (MQ=255) taATGGGCTGACTCAGCTGCATGACAAGAACCAATAAATAGACGCCCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATGACCTTGAAAACACTTAACAAAAAGAAACATACAGCGCTTACAAGAAGAGCTc < 1:244955/138‑1 (MQ=255) gggCTGACTCAGCTGCATGACAAGAAGCAATAAATAGACGCCCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATg > 8:63942/1‑82 (MQ=255) gggCTGACTCAGCTGCATGACAAGAAGCAATAAATAGACGCCCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATg < 7:63942/82‑1 (MQ=255) gggCTGACTCAGCTGCATGACAAGAAGCAATAAATAGACGCCCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATGACGTTGAAAACACTTAACAAAAAGAAACATACAGCGCTTACAAGAAGAGCTCCCTg > 8:141587/1‑138 (MQ=255) gggCTGACTCAGCTGCATGACAAGAAGCAATAAATAGACGCCCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATGACCTTGAAAACACTTAACAAAAAGAAACATACAGCGCTTACAAGAAGAGCTCCCTg > 7:142968/1‑138 (MQ=255) gctcTGACTCAGCTAGATGAAAATAAAAAATAAATACACGCCCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATGACCTTGAAAACACTTAACAAAAAGAAACATACAGCGCTTACAAGAAGAGCTCCCTGc < 2:398507/136‑1 (MQ=255) gtgttaCTCAGCTGCATGACAAGAAGAAAAAAATACACGGCCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATGACCTTGAAAACACTTAACAAAAAGAAACATACAGCGCTTACAAGAAGAGCTCCCTGc < 8:142968/133‑1 (MQ=255) ggCTGAATCAGCTGCATGACAATAAGAAATAAATATACGCCAGTTAAAAAAGTAGTAAACAATTTTGCAAGTCTGTCAATGACCTTGAAAACACTTAACAAAAAGAAACATACAGCGCTTACAAGAAGAGCTCCCTGc > 1:39184/1‑138 (MQ=255) aCTCCGCTGCATGACAAGAAGCAATAAATAGACGCCCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATGACCTTGAAAACACTTAACAAAAAGAAACATACAGCGCTTACAAGAAGAGCTCCCTGCAAAccc > 1:111135/1‑139 (MQ=255) aCTCAGCTGCATGACAAGAAGCAATAAATAGACGCCCGTTACACCCGGAGTAAACAATTTTGCAAGTCTGTCAATGACCTTGAAAACACTTAACAAAAAGAAACATACAGCGCTTACAAGAAGAGCTCCCTGCAAcccc > 1:396224/1‑139 (MQ=255) aCTCAGCTGCATGACAAGAAGCAATAAATAGACGCCCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATGACCTTGAAAACACTTAACAAAAAGAAACATACAGCGCt < 5:175847/114‑1 (MQ=255) aCTCAGCTGCATGACAAGAAGCAATAAATAGACGCCCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATGACCTTGAAAACACTTAACAAAAAGAAACATACAGCGCt > 6:175847/1‑114 (MQ=255) aCTCAGCTGCATGACAAGAAGCAATAAATAGACGCCCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATGACCTTGAAAACACTTAACAAAAAGAAACATACAGCGCTTACAAGAAGAGCTCCCTGCAAAccc > 8:266641/1‑139 (MQ=255) aGCTGCATGACAAGAAGCAATAAATAGACGCCCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATGACCTTGAAAACACTTAACAAAAAGAAACATACAGCGCTTACAAGAAGAGCTCCCTGCAAACCCCg < 6:156457/137‑1 (MQ=255) gcagCAAGACAAGAAGCAATAAATAGACGCCCGTTACAACCGGAGTAAACAATTTAGCAAGTCTGTCAATGACCTTGAAAACAAATAACAACAAGAAACATACAGCGCTTACAAGAATAGCTCCCTGCAAACCCCGCAg > 2:268236/4‑139 (MQ=255) gCTGCATGACAAGAAGCAATAAATAGACGCCCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATGACCTTGAAAACACTTAACAAAAAGAAACATACAGCGCTTAc < 5:106442/112‑1 (MQ=255) gCTGCATGACAAGAAGCAATAAATAGACGCCCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATGACCTTGAAAACACTTAACAAAAAGAAACATACAGCGCTTAc > 6:106442/1‑112 (MQ=255) gCTGCATGACAAGAAGCAATAAATAGACGCCCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATGACCTTGAAAACACTTAACAAAAAGAAACATACAGCGCTTACAAGAAGAGCTCCCTGCAAACCCCGCAg > 2:181260/1‑139 (MQ=255) gCTGCATGACAAGAAGCAATAAATAGACGCCCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATGACCTTGAAAACACTTAACAAAAAGAAACATACAGCGCTTACAAGAAGAGCTCCCTGCAAACCCCGCAg > 2:114195/1‑139 (MQ=255) cggCATGACAACACCCAAAAAATCGACGCCCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATGACCTTGAAAACACTTAACAAAAAGAAACATACAGCGCTTACAAGAAGAGCTCCCTGCAAACCCCGCAgt < 3:270198/137‑1 (MQ=255) cTGCATGACAAGAAGCAATAAATAGACGCCCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATGACCTTGAAAACACTTAACAAAAAGAAACATACAGCGCTTACAAGAAGAGCTCCCTGCAAACCCCGCAgt > 8:246348/1‑139 (MQ=255) cTGCATGACAAGAAGCAATAAATAGACGCCCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATGACCTTGAAAACACTTAACAAAAAGAAACATACAGCGCTTACAAGAAGAGCTCCCTGCAAACCCCGCAgc > 1:398507/1‑138 (MQ=255) caagaaaagaagAAATAAATACACGACCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATGACCTTGAAAACACTTAACAAAAAGAAACATACAGCGCTTACAAGAAGAGCTCCCTGCAAACCCCGCAgtg < 2:103087/131‑1 (MQ=255) cATGACAAGACGCAATAAATAGACGCCCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATGACCTTGAAAACACTTAACAAAAAGAAACATACAGCGCTTACAAGAAGAGCTCCCTGCAAACCCCGCAgtg < 6:142383/137‑1 (MQ=255) aTGACAAGAAGCAATAAATAGACGCCCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATGACCTTGAAAACACTTAACAAAAAGAAACATACAGCGCTTACAAGAAGAGCTCCCTGCAAACCCCGCAgtgtgt > 1:103087/1‑139 (MQ=255) caccAATAACAAGACTCCAGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATGACCTTGAAAACACTTAACAAAACGAAACATACAGCGCTTACAAGAAGAGCTCCCTGCAAACCACGCAGTGTGTCTCCatat < 2:39184/136‑1 (MQ=255) gCAATAAATAGACGCCCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATGACCTTGAAAACACTTAACAAAAAGAAACATACAGCGCTTACAAGAAGAGCTCCCTGCAAACCCCGCAGTGTGTCTCCATATGt < 7:246348/139‑1 (MQ=255) | GCAGCGATCACAACAAGCGCCAGGCCAAATACAAGACCGTCCACCCCGTCCTGTCGATAACCGATTCCGAGAATTCCTTTTATAATGGGCTGACTCAGCTGCATGACAAGAAGCAATAAATAGACGCCCGTTACAACCGGAGTAAACAATTTTGCAAGTCTGTCAATGACCTTGAAAACACTTAACAAAAAGAAACATACAGCGCTTACAAGAAGAGCTCCCTGCAAACCCCGCAGTGTGTCTCCATATGT > CP006881/3713129‑3713379 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |