Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,723,045 T→C 100% intergenic (‑159/‑142) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,723,0450TC100.0% 354.2 / ‑6.6 97intergenic (‑159/‑142)U712_19190/U712_19195PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (34/60);  minor base A (1/2);  total (35/62)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCC‑TTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAAC‑T‑GTTTTTT‑AGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAG  >  CP006881/3722909‑3723174
                                                                                                                                         |                                                                                                                                    
aaCAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCC‑TTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAATAATACGCTTTCATCATAAATCAt                                                                                                                                    >  7:330392/1‑139 (MQ=255)
     aaaaGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCC‑TTTTTCATTTACGCAAATTTTAGTTCTTGCATGCTTTAAGAGTCACAATCACGATTATAAAGATTAAGAATACGCTTTCATCATAAATCATGATAg                                                                                                                               >  3:269598/1‑139 (MQ=255)
                    cGCCAACGAATAAAGGACACAGCGATCC‑TTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑ta                                                                                                               >  1:271218/1‑138 (MQ=255)
                         aCGAATAAAGGACACAGCGATCC‑TTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑T‑Attttt                                                                                                         >  2:395201/1‑139 (MQ=255)
                                     cacaGCGATCC‑TTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAg                                                                                             >  4:112945/1‑139 (MQ=255)
                                     cacaGCGATCC‑TTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAg                                                                                             >  6:155834/1‑139 (MQ=255)
                                      acaGCGATCC‑TTTTTCATTTACTCAAATTTTATGTCTTGCCTGCTTTTCCAGTCACAATACAGCTTATTCAGATTAAGAATACGCTTTAATCATAAATCATGATAGCGTTTTCGTCATC‑TATTTTTTT‑AGTTAAATAGt                                                                                            <  4:269598/139‑1 (MQ=255)
                                      acaGCGATCC‑TTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCTTCAAC‑TATTTTTTT‑AGTTAAATAGt                                                                                            >  1:361582/1‑139 (MQ=255)
                                      acaGCGATCC‑TTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑ATTTAAATAGt                                                                                            >  7:311673/1‑139 (MQ=255)
                                      acaGCGATCC‑TTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGt                                                                                            <  2:58705/139‑1 (MQ=255)
                                      acaGCGATCC‑TTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGt                                                                                            >  1:256867/1‑139 (MQ=255)
                                      acaGCGATCC‑TTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGt                                                                                            <  3:38709/139‑1 (MQ=255)
                                      acaGCGATCC‑TATTTCATTTACGCAAATTTTAGGTCTTGCCTGCATTACCAGTCACAATCCAGCTTATTCATATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGt                                                                                            <  5:316929/139‑1 (MQ=255)
                                       caGCGATCCTTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGt                                                                                            <  6:164619/139‑1 (MQ=255)
                                       caGCGATCC‑TTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGtt                                                                                           <  4:239392/139‑1 (MQ=255)
                                        aGCGATCC‑TTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGttt                                                                                          >  7:110503/1‑139 (MQ=255)
                                        aGCGATCC‑TTTTTCATTTACGAAAATTTTAGGTCTTGCCTCCAATACCAGTAACAATCAAGCTTATTCAGATAAAGAATACTCTATCATAATAAAAAAAGATATCGTATACGTCAACAA‑ATTATTT‑AGTTAAAGAGtt                                                                                           >  8:110573/1‑138 (MQ=255)
                                          cGATCC‑TTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGtt                                                                                           >  1:241668/1‑136 (MQ=255)
                                          cGATCC‑TTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGtt                                                                                           <  2:241668/136‑1 (MQ=255)
                                             tCC‑TTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATat                                                                                     <  6:79125/139‑2 (MQ=255)
                                             tCC‑TTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATat                                                                                     <  1:404720/139‑2 (MQ=255)
                                             tCC‑TTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATa                                                                                      >  2:350254/1‑138 (MQ=255)
                                             tCC‑TTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATa                                                                                      >  1:310195/1‑138 (MQ=255)
                                             tCC‑TTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCAGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TAATTTTTT‑AGTTAAATTGTTTGATa                                                                                      >  5:27527/1‑138 (MQ=255)
                                                   tttCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAAcat                                                                                >  8:173450/1‑137 (MQ=255)
                                                   tttCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAAcat                                                                                >  4:297240/1‑137 (MQ=255)
                                                                           ttGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTaaaaatt                                                         >  1:306347/1‑136 (MQ=255)
                                                                           ttGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTaaaaatt                                                         >  3:126044/1‑136 (MQ=255)
                                                                            tGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaa                                                       >  7:193792/1‑138 (MQ=255)
                                                                               cTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATtg                                                    <  5:396534/139‑1 (MQ=255)
                                                                                  cTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATtgtg                                                  <  3:112945/138‑1 (MQ=255)
                                                                                  cTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATtgtg                                                  <  1:150189/138‑1 (MQ=255)
                                                                                     tACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaa                                               <  5:350459/138‑1 (MQ=255)
                                                                                       ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                             <  3:331023/138‑3 (MQ=255)
                                                                                       ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                             <  4:399294/138‑3 (MQ=255)
                                                                                       ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                             <  2:234471/138‑3 (MQ=255)
                                                                                       ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATAc                                            <  5:276562/139‑1 (MQ=255)
                                                                                       ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCAAGATAGCGGTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                             <  7:312935/138‑3 (MQ=255)
                                                                                       ccAGTCACAATCCCGCTTAATCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                             <  4:372030/138‑3 (MQ=255)
                                                                                        cactCACAATCCCCCTTATTCATTTTAAGAATAAGCTTTCATCAAAAATCATGATATCGTTTTAGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACt                                           <  8:311673/136‑1 (MQ=255)
                                                                                           tCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                        <  8:110503/139‑1 (MQ=255)
                                                                                            cacaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                        >  3:87428/1‑138 (MQ=255)
                                                                                            cacaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                        <  4:87428/138‑1 (MQ=255)
                                                                                             acaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTcaca                                      <  2:306347/139‑1 (MQ=255)
                                                                                             acaATCCAGCTTATTCAGATTAAGAATACTCTTTCATCATTAATAATGATAGAGATTTCGTCAAG‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAATATTACTTGTGATTTACTTcaca                                      <  7:110573/139‑1 (MQ=255)
                                                                                                aTCCCTCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   <  6:351319/139‑1 (MQ=255)
                                                                                                aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACATAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   <  2:361582/139‑1 (MQ=255)
                                                                                                aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   <  8:330392/139‑1 (MQ=255)
                                                                                                aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   <  5:155834/139‑1 (MQ=255)
                                                                                                aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   <  8:102037/139‑1 (MQ=255)
                                                                                                aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   <  5:73996/139‑1 (MQ=255)
                                                                                                aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   <  1:336095/139‑1 (MQ=255)
                                                                                                aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   <  1:350254/139‑1 (MQ=255)
                                                                                                aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   <  8:268356/139‑1 (MQ=255)
                                                                                                aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   <  8:203255/139‑1 (MQ=255)
                                                                                                aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   <  2:179576/139‑1 (MQ=255)
                                                                                                aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   <  2:271218/139‑1 (MQ=255)
                                                                                                aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   <  3:68585/139‑1 (MQ=255)
                                                                                                aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   <  7:205829/139‑1 (MQ=255)
                                                                                                aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑T‑ATTTTTTCAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   <  7:347147/139‑1 (MQ=255)
                                                                                                aTCCCGCTTATTCAGATTAAGAATACGCTATCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   <  4:64908/139‑1 (MQ=255)
                                                                                                aTCCCGCTTATTCAGATTAAGAATACGCTATCATCATAAATCATGATAGCGATTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAATAATTAATTGTGAAATACTTCACAata                                   <  1:15353/139‑1 (MQ=255)
                                                                                                aTCCCGCTTAATCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTGAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   <  8:211914/139‑1 (MQ=255)
                                                                                                aTCCCGCTTAATCAGATTAAGAATACGCTTTCATCATAAATAATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   <  6:109188/139‑1 (MQ=255)
                                                                                                aTCCCGCGTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   <  6:398510/139‑1 (MQ=255)
                                                                                                aTCACGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   <  7:26211/139‑1 (MQ=255)
                                                                                                   ccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                 <  8:396117/138‑1 (MQ=255)
                                                                                                   ccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                 <  8:159059/138‑1 (MQ=255)
                                                                                                   ccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                 <  2:310195/138‑1 (MQ=255)
                                                                                                    cGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGt                               >  8:93369/1‑139 (MQ=255)
                                                                                                    cGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGt                               >  1:261356/1‑139 (MQ=255)
                                                                                                     gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                              <  7:173450/139‑1 (MQ=255)
                                                                                                     gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                              <  4:330610/139‑1 (MQ=255)
                                                                                                     gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                              <  3:297240/139‑1 (MQ=255)
                                                                                                     gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                              <  4:321613/139‑1 (MQ=255)
                                                                                                     gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                              <  2:261356/139‑1 (MQ=255)
                                                                                                      cTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTATTATATAACATGTAGACAAAACTTCGTAAAAATTAATTGTGAAAGACATACCAATATCGTg                              >  4:235508/1‑138 (MQ=255)
                                                                                                         aTTCAGATTAAGAATACGCTTTCATCCTAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCt                            <  3:235508/137‑1 (MQ=255)
                                                                                                           tCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   <  3:293655/128‑1 (MQ=255)
                                                                                                           tCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   >  4:293655/1‑128 (MQ=255)
                                                                                                           tCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATAc                        >  1:85823/1‑139 (MQ=255)
                                                                                                             aGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACt                       <  8:61899/138‑1 (MQ=255)
                                                                                                               aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCtata                         >  3:115529/1‑134 (MQ=255)
                                                                                                               aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCtata                         <  4:115529/134‑1 (MQ=255)
                                                                                                                         cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   <  2:407237/114‑1 (MQ=255)
                                                                                                                         cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   >  1:407237/1‑114 (MQ=255)
                                                                                                                         cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCAt           >  6:374575/1‑138 (MQ=255)
                                                                                                                         cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCAt           >  1:368714/1‑138 (MQ=255)
                                                                                                                         cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑T‑ATTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCAtg          >  7:266856/1‑137 (MQ=255)
                                                                                                                              tcatcaTAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   <  7:276455/109‑1 (MQ=255)
                                                                                                                              tcatcaTAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   >  8:276455/1‑109 (MQ=255)
                                                                                                                               catcatAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                              >  3:146193/1‑113 (MQ=255)
                                                                                                                               catcatAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                              <  4:146193/113‑1 (MQ=255)
                                                                                                                                atcatAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaaga     >  8:366725/1‑137 (MQ=255)
                                                                                                                                 tcatAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaagaaag  <  5:374575/139‑1 (MQ=255)
                                                                                                                                    tAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   <  3:19186/103‑1 (MQ=255)
                                                                                                                                    tAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   >  4:19186/1‑103 (MQ=255)
                                                                                                                                         |                                                                                                                                    
AACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCC‑TTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAAC‑T‑GTTTTTT‑AGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAG  >  CP006881/3722909‑3723174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑