Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	924,071	(A)5→6	intergenic (‑51/‑228)	U712_04490 ← / → U712_04495	Vegetative catalase/Aliphatic sulfonates import ATP‑binding protein SsuB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	924,066	1	.	A	100.0%	110.3 / NA	32	intergenic (‑46/‑233)	U712_04490/U712_04495	Vegetative catalase/Aliphatic sulfonates import ATP‑binding protein SsuB
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (13/19);  total (13/19)

AAGTTGGTCCGCGAGAACCGGCAGTCATTGAGTTTTGATTATCTCCAACCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGT‑AAAAAGTC‑AATATTATTTTATAATTATTATAAAATAGTTATCTCTATTAATCGATATATAGTATCGAAATTGTTGAAAAACCTTGTTGTAACAGCTTTTTATGACACTTAATAATTTTTGGAGAAATAAT  >  CP006881/923933‑924196                                                                                                                                       |                                                                                                                                    aaGTTGGTCCGCGAGAACCGGCAGTCATTGAGTTTTGATTATCTCCAACCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGT‑aaaa                                                                                                                                 <  7:85911/138‑1 (MQ=255)  aGTTGGTCCGCGAGAACCGGCAGTCATTGAGTTTTGATTATCTCCAACCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGT‑aaaa                                                                                                                                 >  1:266878/1‑137 (MQ=255)  aGTTGGTCCGCGAGAACCGGCAGTCATTGAGTTTTGATTATCTCCAACCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGT‑aaaa                                                                                                                                 >  7:397581/1‑137 (MQ=255)    ttGGTCCGCGAGAACCGGCAGTCATTGAGTTTTGATTATCTCCAACCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGT‑aaaaaagt                                                                                                                             <  3:147909/139‑4 (MQ=255)         gcgcgAGAACCGGCAGTCATTGAGGTTTGATTATCTCCAACCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATCTCATCTCTAAGTAAAAAAGTC‑Aata                                                                                                                        <  2:221388/138‑1 (MQ=255)         ccGCGAGAACCGGCAGTCATTGAGTTTTGATTATCTCCAACCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTC‑Aata                                                                                                                        <  8:397581/139‑1 (MQ=255)         ccGCGAGAACCGGCAGTCATTGAGTTTTGATTATCTCCAACCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTC‑Aata                                                                                                                        <  6:59175/139‑1 (MQ=255)              agaACCGGCAGTCATTGAGTTTTGATTATCTCCAACCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTC‑AATattatt                                                                                                                   >  6:381804/1‑139 (MQ=255)                       actCATTGAGTTTTGATTATCTCCAACCCGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCTCCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTC‑AATATTATTTTATAatta                                                                                                          <  4:381224/137‑1 (MQ=255)                               aGTTTTGATTATCTCCAACCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTC‑AATATTATTTTATAATTATTATaaa                                                                                                   <  2:34274/138‑1 (MQ=255)                                      aTTATCTCCAACCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTTAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTC‑AATATTATTTTATAATTATTATAAAATAGTTAt                                                                                           <  5:381804/139‑1 (MQ=255)                                      aTTATCTCCAACCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTC‑AATATTATTTTATAATTATTATAAAATAGTTAt                                                                                           <  5:258686/139‑1 (MQ=255)                                              cAACCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTC‑AATATTATTTTATAATTATTATAAAATAGTTATCTCTAtt                                                                                    <  5:212996/138‑1 (MQ=255)                                               aaCCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTC‑AATATTATTTTATAATTATTATAAAATAGTTATCTCTATTaa                                                                                  <  1:303777/139‑1 (MQ=255)                                               aaCCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTC‑AATATTATTTTATAATTATTATAAAATAGTTATCTCTATTaa                                                                                  <  3:272356/139‑1 (MQ=255)                                               aaCCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTC‑AATATTATTTTATAATTATTATAAAATAGTTATCTCTATTaa                                                                                  <  4:393329/139‑1 (MQ=255)                                                aCCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTCAAATATTATTTTATAATTATTATAAAATAGTTATCTCTATTaa                                                                                  <  3:402875/139‑1 (MQ=255)                                                aCCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTC‑AATATTATTTTATAATTATTATAAAATAGTTATCTCTATTaa                                                                                  >  1:201998/1‑138 (MQ=255)                                                aCCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTC‑AATATTATTTTATAATTATTATAAAATAGTTATCTCTATTAAt                                                                                 <  8:377707/139‑1 (MQ=255)                                                 tcGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATGACCTCTTTGAATTTATTATTACATAATATCATGTCTTAGTAAAAAAGTC‑AATATTATTTTATAATTATTATAAAATAGTTATCTCTATTaa                                                                                  <  4:307789/136‑1 (MQ=255)                                                  cGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGGC‑AATATTATTTTATAATTATTATAAAATAGTTATCTCTATTAATCg                                                                               <  2:103473/139‑1 (MQ=255)                                                     aGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTC‑AATATTATTTTATAATTATTATAAAATAGTTATCTCTATTaa                                                                                  <  8:204996/133‑1 (MQ=255)                                                     aGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTC‑AATATTATTTTATAATTATTATAAAATAGTTATCTCTATTaa                                                                                  >  7:204996/1‑133 (MQ=255)                                                       cgcCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTC‑AATATTATTTTATAATTATTATAAAATAGTTATCTCTATTAATCGatata                                                                          <  2:266878/139‑1 (MQ=255)                                                       cgcCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAACTAAAAAAGTC‑AATATTAATTTATAATTATTATAAAATAGTTATCTCTATTAATCGatata                                                                          <  2:343327/139‑1 (MQ=255)                                                         ccccAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTC‑AATATTATTTTATAATTATTATAAAATAGTTATCTCTTTTAATCTAtattta                                                                        >  6:14919/1‑139 (MQ=255)                                                          cccAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTC‑AATATTATTTTATAATTATTATAAAATAGTTATCTCTATTAATCGATATATAg                                                                       >  7:141089/1‑139 (MQ=255)                                                                        aGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTC‑AATATTATTTTATAATTATTATAAAATAGTTATCTCTATTAATCGATATATAGTATCGAAAttgtt                                                          >  2:73200/1‑138 (MQ=255)                                                                             aTTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTC‑AATATTATTTTATAATTATTATAAAATAGTTATCTCTATTAATCGATATATAGTATCGAAATTGTTGaaaaa                                                    >  1:321042/1‑139 (MQ=255)                                                                                            aTCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTC‑AATATTATTATATAATTATTATAAAATAGTTATCTCTATTAATCGATATATAGTATCTAAATTGTTGAAAAAACTTGTTGTAACatc                                     >  8:120627/1‑137 (MQ=255)                                                                                                 ctctTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTC‑AATATTATTTTATAATTATTATAAAATAGTTATCTCTATTAATCGATATATAGTATCGAAATTGTTGAAAAACCTTGTTGTAACAGCtttt                                 >  2:8356/1‑138 (MQ=255)                                                                                                      ggAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTC‑AATATTATTTTATAATTATTATAAAATAGTTATCTCTATTAATCGATATTTAGTATCGAAATTGTTGAAAAACCTTGTTGTAACAGCTTTTTATGa                            >  4:105504/1‑138 (MQ=255)                                                                                                      ggAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTC‑AATATTATTTTATAATTATTATAAAATAGTTATCTCTATTAATCGATATATAGTATCGAAATTGTTGAAAAACCTTGTTGTAACAGCTTTTTATGa                            <  1:73200/138‑1 (MQ=255)                                                                                                                              tctcTAAGTAAAAAAGTC‑AATATTATTTTATAATTATTATAAAATAGTTATCTCTATTAATCGATATATAGTATCGAAATTGTTGAACAACCTTGTTGTACCAGCTTTTTATGACACTTAATAATTTTTGGAGAaataa   >  5:40176/1‑139 (MQ=255)                                                                                                                              tctcTAAGTAAAAAAGTC‑AATATTATTTTATAATTATTATAAAATAGTTATCTCTATTAATCGATATATAGTATCGAAATTGTTGAAAAACCTTGTTGTAACAGCTTTTTATGACACTTAATAATTTTTGGAGAaataa   >  5:360920/1‑139 (MQ=255)                                                                                                                               ctctAAGTAAAAAAGTC‑AATATTATTTTATAATTATTATAAAATAGTTATCTCTATTAATCGATATATAGTATCGAAATTGTTGAAAAACCTTGTTGTAACAGCTTTTTATGACACTTAATAATTTTTGGAGAaataat  >  5:274847/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                    AAGTTGGTCCGCGAGAACCGGCAGTCATTGAGTTTTGATTATCTCCAACCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGT‑AAAAAGTC‑AATATTATTTTATAATTATTATAAAATAGTTATCTCTATTAATCGATATATAGTATCGAAATTGTTGAAAAACCTTGTTGTAACAGCTTTTTATGACACTTAATAATTTTTGGAGAAATAAT  >  CP006881/923933‑924196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑