Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,182,935 T→C A103A (GCA→GCG)  U712_16370 ← Thioredoxin‑like protein yusE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,182,9350TC97.6% 303.5 / ‑3.1 84A103A (GCA→GCG) U712_16370Thioredoxin‑like protein yusE
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (53/29);  total (54/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGCTTTCTCCATATGTACAACCTCCTATTTGTTGGTCATACATACACATTTCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGATGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCATATCCTCTTTCGACCATCTTTCCGCCTTTAAACAGCAGAAAACAAGGGACGCTTTCAATTTGATACGCTTTTGCGA  >  CP006881/3182804‑3183068
                                                                                                                                   |                                                                                                                                     
ccGCTTTCTCCATATGTACAACCTCCTATTTGTTGGTCATACATACACATTTCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCgg                                                                                                                                   >  3:372256/1‑136 (MQ=255)
 cGCTTTCTCCATATGTACAACCTCCTATTTGTTGGTCATACATACACATTTCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCgg                                                                                                                                   >  2:274364/1‑135 (MQ=255)
 cGCTTTCTCCATATGTACAACCTCCTATTTGTTGGTCATACATACACATTTCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAga                                                                                                                               <  5:403163/139‑1 (MQ=255)
                tACAACCTCCTATTTGTTGGTCATACCTACACATTTCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGAt                                                                                                                <  5:111143/139‑1 (MQ=255)
                tACAACCTCCTATTTGTTGGTCATACATACACATTTCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGAt                                                                                                                <  4:372256/139‑1 (MQ=255)
                tACAACCTCCTATTTGTTGGTCATACATACACATTTCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGAt                                                                                                                <  1:274364/139‑1 (MQ=255)
                tACAACCTCCTATTTGTTGGTCATACATACACATTTCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGAt                                                                                                                >  7:164819/1‑139 (MQ=255)
                tACAACCTCCTATTTGTTGGTCATACATACACATTTCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAAATTCATTGGATATGCTGAGAAATATGTCATAAATGTGACGCGGAAGATGATATTTGTATGa                                                                                                                 >  5:340600/1‑138 (MQ=255)
                 aCAACCTCCTATTTGTTGGTCATACATACACATTTCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGAt                                                                                                                <  8:237014/138‑1 (MQ=255)
                  cAACCTCCTATTTGTTGGTCATACATACACATTTCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGTACGCGGAAGATGATTTTTGTTTGAt                                                                                                                >  2:76834/1‑137 (MQ=255)
                  cAACCTCCTATTTGTTGGTCATACATACACATTTCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGAt                                                                                                                >  4:269287/1‑137 (MQ=255)
                        cctATTTGTTGGTCATACATACCCATTTCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGTACGCGGAAGATGATTTTTTTTTGATCAGCTCa                                                                                                         >  7:144364/1‑138 (MQ=255)
                        cctATTTGTTGGTCATACATACACATTTCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCa                                                                                                         >  8:358760/1‑138 (MQ=255)
                         ctATTTGTTGGTCATACATACACATTTCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCata                                                                                                       <  7:246430/139‑1 (MQ=255)
                              tgttgGTCATACATACACATTTCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGATCATataaa                                                                                                   >  7:347308/1‑138 (MQ=255)
                               gttgGTCATACATACACATTTCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATataaat                                                                                                  >  4:94327/1‑138 (MQ=255)
                               gttgGTAATACATACACATTTCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGAAAATTTCATTGGATATGCTTAGAAATATGTCATAAATGGGACGCGTAAGATGATTTTTGTTTGATCAGCTCATataaaa                                                                                                  <  6:173935/138‑2 (MQ=255)
                                ttgGTCATACATACACATTTCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATataaataa                                                                                                >  1:171497/1‑139 (MQ=255)
                                 tgGTCATACATACACATTTCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGAt                                                                                                                <  2:90808/122‑1 (MQ=255)
                                 tgGTCATACATACACATTTCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGAt                                                                                                                >  1:90808/1‑122 (MQ=255)
                                 tgGTCATACATACACATTTCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATataaat                                                                                                  >  6:66931/1‑136 (MQ=255)
                                 tgGTCATACATAAACATTTCTCTCTTTGTCGCAAGAACACCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATATCATAAATGTGACGCTGAAGATGATATTTGTTTTATCAGCTCATataaat                                                                                                  >  2:26876/1‑136 (MQ=255)
                                  ggTCATACATACACATTTCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAg                                                                                               <  3:94327/138‑1 (MQ=255)
                                   gTCATACATACACATTTCTCTCTTTGTCGCAAGACCCCCTTTATAAAACGAACGACAATTTCATTGCATATGCTGAGAAATATGTCATAAATGGTACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGa                                                                                              >  1:222941/1‑138 (MQ=255)
                                   gTCATACATACACATTTCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGa                                                                                              >  4:327615/1‑138 (MQ=255)
                                        acatacaCATTTCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGa                                                                                        <  3:269287/139‑1 (MQ=255)
                                           tacaCATTTCTCTCTTTGTCGCAACAACCGATTTATAAAGCTCACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGAATTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATg                                                                                     <  5:47839/139‑1 (MQ=255)
                                             cacaTTTCTCTCTTTGTCGCAAGAACCCCTTTATAAAACTAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGa                                                                                    <  3:78564/138‑1 (MQ=255)
                                              acaTTTCTCTCTTTGTCGCAAGAACCCCTTTATAAACCGAAAGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGaaa                                                                                  <  8:55492/139‑1 (MQ=255)
                                              acaTTTCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGaaa                                                                                  >  6:167890/1‑139 (MQ=255)
                                              acaTTTCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGaaa                                                                                  >  6:334816/1‑139 (MQ=255)
                                              acaTTTCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGaaa                                                                                  >  7:69045/1‑139 (MQ=255)
                                              acaTTTCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGaaa                                                                                  >  4:221576/1‑139 (MQ=255)
                                                aTTTCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGATGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATg                                                                                >  8:238453/1‑139 (MQ=255)
                                                 tttATCTCTTTGTCTCAAGAACCACTTTATAAAACGAACGACTATTTCATTGGATATGCTGAGAAATATGTCATTAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGc                                                                               <  6:340600/139‑1 (MQ=255)
                                                  ttCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGAt                                                                                                                >  2:27476/1‑105 (MQ=255)
                                                  ttCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGAt                                                                                                                <  5:231984/105‑1 (MQ=255)
                                                  ttCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGAt                                                                                                                >  6:231984/1‑105 (MQ=255)
                                                  ttCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGAt                                                                                                                <  1:27476/105‑1 (MQ=255)
                                                  ttCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGc                                                                               >  3:356309/1‑138 (MQ=255)
                                                  ttCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGc                                                                               >  2:103129/1‑138 (MQ=255)
                                                  ttCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCa                                                                              >  4:179270/1‑139 (MQ=255)
                                                  ttCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCa                                                                              >  3:300451/1‑139 (MQ=255)
                                                  ttCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCa                                                                              >  8:294854/1‑139 (MQ=255)
                                                  ttCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCa                                                                              >  5:43410/1‑139 (MQ=255)
                                                  ttCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCa                                                                              >  5:342286/1‑139 (MQ=255)
                                                  ttCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCAATGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTATTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATgaa                                                                              >  4:69293/1‑137 (MQ=255)
                                                    ctctctTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGTGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCata                                                                            >  8:34020/1‑139 (MQ=255)
                                                        ctTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCATATCct                                                                        >  3:383376/1‑139 (MQ=255)
                                                         tttGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCATATc                                                                          <  1:196324/136‑1 (MQ=255)
                                                                   gAACCCATTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGATGATGATTTTTGTTTGATCAGATCATATAAATATGAAACGGAATGATATGCATATCCTATGTCGAcgc                                                              >  4:49577/1‑136 (MQ=255)
                                                                        ccTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCATATCCTCTTTCGACCATCTTTc                                                        <  8:164819/139‑1 (MQ=255)
                                                                        ccTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCATATCCTCTTTCGACCATCTTTc                                                        <  7:358760/139‑1 (MQ=255)
                                                                           ttATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCATATCCTCTTTCGACCATCTTTCCGc                                                     >  4:199084/1‑139 (MQ=255)
                                                                           ttATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCATATCCTCTTTCGACCATCTTTCCGc                                                     >  7:170310/1‑139 (MQ=255)
                                                                              taAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCATATCCTCTTTCGACCATCTTTCCGCCt                                                   <  3:221576/138‑1 (MQ=255)
                                                                                 aacgaacgACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCATATCCTCTTTCGACCATCTTTCCGCCttaa                                                >  3:266604/1‑136 (MQ=255)
                                                                                 aacgaacgACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCATATCCTCTTTCGACCATCTTTCCGCCTTTa                                                >  6:314078/1‑138 (MQ=255)
                                                                                 aacgaacgACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCATATCCTCTTTCGACCATCTTTCCGCCTTTa                                                >  1:252829/1‑138 (MQ=255)
                                                                                  acgaacgaCAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCATATCCTCTTTCGACCATCTTTCCGCCTTTaaa                                              <  6:389041/139‑1 (MQ=255)
                                                                                   cgaacgaCAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCATATCCTCTTTCGACCATCTTTCCGCCTTTAAAc                                             >  6:167067/1‑139 (MQ=255)
                                                                                     aacgaCAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCATATCCTCTTTCGACCATCTTTCCGCCTTTAAAcag                                           <  2:385180/139‑1 (MQ=255)
                                                                                      acgaCAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCATATCCTCTTTCGACCATCTTTCCGCCTTTAAAcag                                           >  4:753/1‑138 (MQ=255)
                                                                                       cgaCAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCATATCCTCTTTCGACCATCTTTCCGCCTTTAAAcag                                           >  2:161102/1‑137 (MQ=255)
                                                                                            aTTTCATTGGATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCATATCCTCTTTCGACCATCTTTCCGCCTTTAAACAGCAGaaaa                                    <  2:176939/139‑1 (MQ=255)
                                                                                                    ggATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCATATCCTCTTTCGACCATCTTTCCGCCTTTAAACAGCAGAAAACAAGGGa                             <  1:103129/138‑1 (MQ=255)
                                                                                                    ggATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCATATCCTCTTTCGACCATCTTTCCGCCTTTAAACAGCAGAAAACAAGGGa                             <  2:220143/138‑1 (MQ=255)
                                                                                                     gATATGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCATATCCTCTTTCGACCATCTTTCCGCCTTTAAACAGCTGAAAACAAGGGACg                           >  8:193845/1‑139 (MQ=255)
                                                                                                       tatGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCATATCCTCTTTCGACCATCTTTCCGCCTTTAAACAGCAGAAAACAAGGGACGCt                         >  7:213675/1‑139 (MQ=255)
                                                                                                       tatGCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCATATCCTCTTTCGACCATCTTTCCGCCTTTAAACAGCAGAAAACAAGGGACGCt                         >  3:291482/1‑139 (MQ=255)
                                                                                                         tGCTGAGAAATATGTCATAAATGGGATGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCATATCCTCTTTCGACCATCTTTCCGCCTTTAAACAGCAGAAAACAAGGGACGCttt                       <  7:238453/139‑1 (MQ=255)
                                                                                                          gCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCATATCCTCTTTCGACCATCTTTc                                                        >  4:250339/1‑105 (MQ=255)
                                                                                                          gCTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCATATCCTCTTTCGACCATCTTTc                                                        <  3:250339/105‑1 (MQ=255)
                                                                                                           cTGAGAAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCATATCCTCTTTCGACCATCTTTCCGCCTTTAAACAGCAGAAAACAAGGGACGCTTTc                      >  3:2223/1‑138 (MQ=255)
                                                                                                               gaAATATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCATATCCTCTTTCGACCATCTTTCCGCCTTTAAACAGCAGAAAACAAGGGACGCTTTCAAttt                 >  1:302917/1‑139 (MQ=255)
                                                                                                                aaaTATGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACTGAATGAAATGCATATCCTCTTTCGACCATCTTTCCGCCTTTAAACAGCAGAATACAAGGGACGCTTTCAAttt                 >  8:357195/1‑138 (MQ=255)
                                                                                                                   tatGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCATATCCTCTTTCGACCATCTTTCCGCCTTTAAACAGCAGAAAACAAGGGACGCTTTCAATTTGATa             >  6:403118/1‑139 (MQ=255)
                                                                                                                     tGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCATATCCTCTTTCGACCATCTTTc                                                        <  4:78724/94‑1 (MQ=255)
                                                                                                                     tGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCATATCCTCTTTCGACCATCTTTc                                                        >  3:78724/1‑94 (MQ=255)
                                                                                                                     tGTCATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCATATCCTCTTTCGACCATCTTTCCGCCTTTAAACAGCAGAAAACAAGGGACGCTTTCAATTTGATACg           >  3:384557/1‑139 (MQ=255)
                                                                                                                        cATAAATGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCATATCCTCTTTCGACCATCTTTCCGCCTTTAAACAGCAGAAAACAAGGGACGCTTTCAATTTGATACGCtt        >  2:96411/1‑139 (MQ=255)
                                                                                                                             aTGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCATATCCTCTTTCGCCCATCTTTCCGCCTTTAAACAGCAGAAAACAAGGGACGCTTTCAAGTTGATACGCTTTTGc    >  8:108430/1‑138 (MQ=255)
                                                                                                                              tGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCATATCCTCTTTCGACCATCTTTCCGCCTTTAAACAGCAGAAAACAAGGGACGCTTTCAATTTGATACGCTTTTGCGa  <  2:302917/139‑1 (MQ=255)
                                                                                                                              tGGGACGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCATATCCTCTTTCGACCATCTTTCCGCCTTTAAACAGCAGAAAACAAGGGACGCTTTCAATTTGATACGCTTTTGCGa  <  4:291482/139‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                     
CCGCTTTCTCCATATGTACAACCTCCTATTTGTTGGTCATACATACACATTTCTCTCTTTGTCGCAAGAACCCCTTTATAAAACGAACGACAATTTCATTGGATATGCTGAGAAATATGTCATAAATGGGATGCGGAAGATGATTTTTGTTTGATCAGCTCATATAAATAAGAAACGGAATGAAATGCATATCCTCTTTCGACCATCTTTCCGCCTTTAAACAGCAGAAAACAAGGGACGCTTTCAATTTGATACGCTTTTGCGA  >  CP006881/3182804‑3183068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑