| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 3,725,010 | C→T | E53E (GAG→GAA) | U712_19205 ← | Uncharacterized protein ywcI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 3,725,010 | 0 | C | T | 90.7% | 117.8 / ‑5.0 | 43 | E53E (GAG→GAA) | U712_19205 | Uncharacterized protein ywcI |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (4/35); minor base A (3/1); total (7/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.05e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCGCCATGTAAAGATGCGGGCCAGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGC‑AAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCA > CP006881/3724879‑3725138 | aCCGCCATGTAACGTTGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGC‑AAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATaaa < 5:297972/139‑1 (MQ=255)aCCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGC‑AAACATCTTCTTTTATAAAGCGCGGCGGCGTTCGCGTTATGGTTTTGATACTCATTAAACGCCCACCGCCCATCATaa > 5:368300/1‑138 (MQ=255) agCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGC‑AAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAAc < 7:16525/138‑1 (MQ=255) ccATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGC‑AAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGa < 6:273991/139‑1 (MQ=255) ccATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGC‑AAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCGGGTTTTGTTGCTCATTCCCAGCCAGCCGCCATTCATAAAACg > 2:89714/1‑138 (MQ=255) ccATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGC‑AAACATCTTCTTTTATAAAGCGCGGCGGCGTTAGCTTTGAGCTTTTATTACTCATTAAAACCCAACCCCCAATAaaaaaaaa > 6:297972/1‑136 (MQ=255) tGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGC‑AAACATCTTCTTTTATAAAGCGCGGCGGCTTTAGCTTTCTTCTTTTGTTGCTCATTAACAGCCATCCCCCAATCATAAAAACAAACCAGTcc > 8:16525/1‑137 (MQ=255) gCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTcct < 4:108210/138‑2 (MQ=255) cGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGC‑AAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGGTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCtgcg > 1:35259/1‑139 (MQ=255) ggCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGC‑AAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGc < 6:76013/139‑1 (MQ=255) ttctctcTTGCTTTGGGGATTCTTTGATGTTTTGC‑AAAAATCTTCTTTTATAATGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATCCACTGCCATCCGCCTTTCATAAAACGAAACGAGTCCTGCGGCCTGCAc < 4:92805/138‑1 (MQ=255) tcCTGCTTTGGCGATTCCTTGAAGGCTTGC‑AAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCAcagccg > 5:373973/1‑139 (MQ=255) cTGCTTTGGCGATTCCTTGAAGGCTTGC‑AAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCATCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACagccgcc < 8:89524/139‑2 (MQ=255) cTGCTTTGGCGATTCCTTGAAGGCTTGC‑AAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTATTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACagccgcc < 1:129698/139‑2 (MQ=255) cTGCTTTGGAGATTCCTTTAAGGCTTGC‑AAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGATTTCTGCTTTTCTTCCTCCTTCACAGCCATCAGCCATTCATAAAACGAAACGATTCCTGCGGCCTGCACagccgcc < 1:55722/139‑2 (MQ=255) tGGCGATTCCTTGACGGCTTGC‑AAACATCTTCTTTTAAAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCCCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCt < 1:161658/138‑1 (MQ=255) tGGCGATTCCTTGAAGGCTTGC‑AAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCt < 5:120922/138‑1 (MQ=255) ggggATTCCTTGAAGGCTTGC‑AAACATTTTCTTTTAAAACGCGCGGCGGCTTTCGCTTTCTGGTTTTCTTCCTCATTCAAAGCCATCCGCAATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt < 2:122894/136‑1 (MQ=255) ggCGATTCCTTGAAGGCTTGC‑AAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt < 3:32726/139‑1 (MQ=255) ggCGATTCCTTGAAGGCTTGC‑AAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCt < 5:276299/137‑1 (MQ=255) gCGATTCCTTGAAGGCTTGC‑AAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt < 6:373973/138‑1 (MQ=255) gCGATTCCTTGAAGGCTTGC‑AAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTAACAGCCCTCCGCCATTAATAAAACGAAACGAGTACAGCGGCCTGCACACACGCCGTTCTTTg > 4:175954/1‑139 (MQ=255) agacagccTTGAAGGCTTGC‑AAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTg < 3:132888/133‑1 (MQ=255) tCCTTGAAGGCTTGC‑AAACATCTTCTTTTATAAAGCGCTGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTAt < 7:91940/138‑1 (MQ=255) gacggCTTGC‑AAACTGATCATTTTTTCACGCGGGGCGGCTTTCGCTTTCTGCTTTTCTACCTCAATCACAGCCACCCGCCATACATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACg < 2:391580/135‑1 (MQ=255) gAAGGCTTGC‑AAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACg < 4:112540/138‑1 (MQ=255) gAAGGCTTGC‑AAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACg < 1:337114/138‑1 (MQ=255) gAAGGCTTGC‑AAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACg < 3:91949/138‑1 (MQ=255) aaGGCTTGC‑AACCATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt < 2:40568/139‑1 (MQ=255) aGGCTTGC‑AAACATCTTCTTTTATAACGCGCGGCGGGTTTCGCTTTCTGCTTTTCTTCCTCATTCACTGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt < 1:329508/138‑1 (MQ=255) gCTTGC‑AAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg < 6:304222/139‑1 (MQ=255) gCTTGC‑AAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg < 7:257657/139‑1 (MQ=255) gCTTGC‑AAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg < 2:321461/139‑1 (MQ=255) ataAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGc < 1:89714/138‑1 (MQ=255) ataAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGc < 3:397709/138‑1 (MQ=255) ggcggcTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGttttt < 6:160304/139‑1 (MQ=255) cTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCAACCGCCAATCATAAACCGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTc < 5:212173/139‑1 (MQ=255) ctgcttttCTTCCTCATTCACAGCCATCCGCAATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCAc < 1:105502/134‑1 (MQ=255) tcttcCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTACTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTtc < 5:371913/138‑1 (MQ=255) aTTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAg < 4:209330/139‑1 (MQ=255) tCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCTCAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGa < 7:93616/138‑1 (MQ=255) ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca < 7:301646/139‑1 (MQ=255) ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca < 1:56701/139‑1 (MQ=255) | ACCGCCATGTAAAGATGCGGGCCAGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGC‑AAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCA > CP006881/3724879‑3725138 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |