| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 3,725,237 | A→G | intergenic (‑69/‑460) | U712_19205 ← / → U712_19210 | Uncharacterized protein ywcI/Minor extracellular protease vpr |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 3,725,237 | 0 | A | G | 84.6% | 124.4 / ‑1.3 | 52 | intergenic (‑69/‑460) | U712_19205/U712_19210 | Uncharacterized protein ywcI/Minor extracellular protease vpr |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (34/10); minor base C (1/7); total (35/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.37e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGC‑AGC‑C‑T‑TT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCACGGGAAAA‑GACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAAC > CP006881/3725109‑3725353 | cGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGC‑AGC‑CTT‑TT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGaaaa > 6:28077/1‑138 (MQ=255) gTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGC‑AGC‑CTT‑TT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAA‑GACGGCCGAt > 4:63699/1‑139 (MQ=255) ttCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCGTGTTTTTGGCAGGC‑CGC‑C‑TGTT‑T‑A‑GCAGCCCGTTTTAGCAGCCGCAGCCCGCAAGAAAA‑GACGGCCGCtt > 2:383935/1‑139 (MQ=255) ttCATCAAAAAGACAATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCGTTTTGGCCGGC‑AGC‑C‑TATT‑T‑A‑GCAGCCCGTATTCTCCCCCGCAGCCCCCAATAAAA‑GACGGcctcag > 4:112230/1‑135 (MQ=255) caAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGC‑AGC‑CTT‑TT‑T‑A‑GCATCCCCTTTTCTCAGCCGCAGCCCGCAAGAAAA‑GACGGCCGAtttcttt > 3:340290/1‑139 (MQ=255) acacaCCATCCACACGCTCAAATATACAAGCAAAGGTTTAAAAGAAAAACATCCCTCCGTTTCTTTTTGGCAGGC‑AGCAT‑T‑TT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCCGCCCCAAATAAAA‑CACGGCCGAtttcttttct < 6:252234/135‑1 (MQ=255) aaaGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGC‑AGC‑CTT‑TT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAA‑GACGGCCGAtttcttttct > 7:216906/1‑139 (MQ=255) aaaGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGC‑AGC‑CTT‑TT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAA‑GACGGCCGAtttcgttta > 5:347805/1‑137 (MQ=255) aCCATCCACACCCGTAAAGATACACCAACACTTTTCTTTGAAAAACATCCGTCCTCTTTTTTTTGGAAGGC‑AGC‑C‑T‑TT‑T‑ACGCGGCGCGTTTGCTCAGCCCCCGCCCCCACGAAAA‑CTCGGCCGATTTCTTTTCTCGCa < 8:49583/139‑1 (MQ=255) aCCATCCACACCCGAAACGATACAACCAACGGTTTCTTACAAAAACACCCCTCCGCTTCTTTTGGGCAGCC‑AGC‑CTT‑TT‑T‑C‑CCCCCCCGTTTTCTCCCCCCCAGCCCGCAAGAAAA‑GACGGCCGATTTCTTTTCTCGCa < 8:49577/139‑1 (MQ=255) cacaCCCTTAAATATAAAAGCAATGGTTTTATAAAAAAACATCCGTCGGTTTTTTTTTGGCGGGCTGGT‑T‑T‑TT‑T‑A‑GCCGCCGGTTTTCTCCCCCGCCGCGGCAATAAAAA‑CACGGCCGATTTCTTTTCTCGCAAACTAg < 4:340290/139‑1 (MQ=255) acagcccGAAAATAAACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTGTTTTTGGCAGGC‑AGC‑CTT‑TT‑T‑A‑GCAGCCCGTTTTCTCAGCCCCCGCCCGCAATAAAC‑GACGGCCGATTTCTTTTCTCGCAAACTAg < 3:89543/135‑1 (MQ=255) gTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGC‑AGC‑CTT‑TT‑T‑A‑GCAGCCCGTTTTATCAGCCGCAGCCCGCAAGAAAA‑GACGGCCTATTTATTTTGTCGCAAACTAGCggagat > 5:244016/1‑135 (MQ=255) tAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGC‑AGC‑CTT‑TT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAA‑GACGGCCGAGTTCTTTTCTCGCAAACTAGCGGctc > 4:378060/1‑137 (MQ=255) aaGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGC‑AGC‑CTT‑TT‑T‑A‑GCATCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAA‑GACGGCCGATTTCGTTTCTCGCAAACTAGCGGCTCTat > 3:345310/1‑137 (MQ=255) aCAAGCAAACGTTTCATAGAAAAACGTCCCTCCGCTTCTTTTTGGCAGGC‑AGC‑CTT‑TT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAA‑GACGGCCGATTTCTTTTGTGGCAAACAACCGGCGATAGCcaaa > 1:203756/1‑138 (MQ=255) aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGC‑AGC‑CTT‑TT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAA‑GACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCactgt > 4:89543/1‑135 (MQ=255) aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGC‑AGC‑CTT‑TT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAA‑GACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCaaaa > 3:400520/1‑138 (MQ=255) aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGC‑AGC‑CTT‑TT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAA‑GACGGCCGATTTCTTTTCTCGCAAACAAGCGCCTCGAGCaaaa > 6:132349/1‑138 (MQ=255) aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGC‑AGC‑CTT‑TT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAA‑GACGGCCGATTTCTTTTATCGCAAACAAGCCGCTCTAGCaaaa > 4:230857/1‑138 (MQ=255) aaGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGC‑CGC‑CTT‑TT‑T‑A‑GCAGCCCGTTTTCTCCGCCGCAGCCCGCAAGAAAA‑GACGGCCGATTTCGTTTGTCCGAAACTAGCGGCGAGAGCAAAAca > 1:122685/1‑137 (MQ=255) aaGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGC‑AGC‑CTT‑TT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCCAGAAAA‑GACGGCCGATTTCGTTTCTCGCAAACGAGCGGCTCTAGCAAAACTc > 8:47171/1‑139 (MQ=255) aaGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGC‑AGC‑CTT‑TT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAA‑GACGGCCGATTTCTTTTCTCGCAAACTAGc > 4:403585/1‑123 (MQ=255) aaGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGC‑AGC‑CTT‑TT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAA‑GACGGCCGATTTCTTTTCTCGCAAACTAGc < 3:403585/123‑1 (MQ=255) aaGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGC‑AGC‑CTT‑TT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAA‑GACGGCCGATTTCTTTTCTACCAAACTACCGGCTCAAGCAAAACt > 2:41973/1‑138 (MQ=255) gaaaaCGTATCAAACAAAACCCTCCCTCCGTTTATTTCGGCCACGC‑AGC‑C‑T‑TTCT‑A‑TCCGCCGGTTCTCTCAGCCGCAGCCCCCATCAACA‑GACGCCCGATTTCTTTTCTCGCAAACAAGCGGCTCTAGCAAACCTCAt < 8:323905/137‑1 (MQ=255) gCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGC‑AGC‑CTT‑TT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAA‑GACGGCCGATTTCTTTTCTCGCAAACTAGCGGCGCTAGCAAAACTCAt > 6:399406/1‑139 (MQ=255) gCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGC‑AGC‑CTT‑TT‑T‑A‑GCAGCCCGTTTTCTCAGCCCCAGCCCGCAAGAAAA‑GACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt < 7:68126/139‑1 (MQ=255) gCAAACGTTTCATAGAAAAACATCCCTCCGCTTATTTTTGGCAGGC‑AGC‑CTT‑TT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAA‑GACGGCCGATTTCTTTTCTCGCAAACTAGCGCCTCTAGCAAAACTCAt > 5:172020/1‑139 (MQ=255) aaaCTTTCCAAATAAAAACATCCCTCCGCTTCTTTTTGGCAGCC‑AGC‑C‑T‑TT‑TAA‑GCAGCCCGTTTTCTCAGCCGCCGCCCGAAACAAAA‑GACGGCCGATTTATTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGa < 8:216906/139‑1 (MQ=255) aaaCGTTTCTTATAAAAACATCCCTCCGCTTCTTTTTGGCTGGC‑AGC‑CTT‑TT‑T‑A‑GCAGCCCTTTTTCTCCGCCGCAGCCCGCAAGAAAC‑GACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGa < 6:347805/139‑1 (MQ=255) ccGTTTCATACAAAAACATCCCTCCGCTTTTTTTTGGCAGGC‑AGC‑CTT‑TT‑T‑A‑GCAGCCCGTTTTCGCAGCCGCAGCCCGAAACAAAA‑GACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct < 1:41973/138‑1 (MQ=255) ccGTCTCATTGAAAAACAACCCTCGGCTGCTTTTTGGCCGGC‑AGC‑CTT‑TT‑T‑T‑GCCGCCCTTTTGCACCCCCGCCCCCCGAAACAAAC‑CACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct < 4:77369/138‑1 (MQ=255) acttttCATACAAAAACACCCCTCCGCTTCTTTTTGGCTGGC‑AGCAT‑T‑TT‑T‑A‑GCAGCCCTTTTCCTCACCCGCCCCCCCAAATAAAA‑CACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct < 5:377912/136‑1 (MQ=255) aCGTTTCATATAAAAACACCCCTCCGTGTATTTTTGGCAGGC‑TGC‑C‑T‑TT‑T‑ATGCCGCCCGTTTGCTCCGCCGCTCCCCGCAACAAAC‑CACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct < 4:93119/139‑1 (MQ=255) aCGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGC‑AGC‑CTT‑TT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAA‑GACGGCCGCTTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAcg > 5:115785/1‑138 (MQ=255) aCGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGC‑AGC‑CTT‑TT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAA‑GACGGCCGATTTCTTTTCTCGCAAACTAGCGGCGCTAGCAAAACGCATGAct > 7:368816/1‑139 (MQ=255) aCGTTTATTACAAAAACACCCCCCCGCTTGTTTTTGCCCGCC‑CGC‑CTT‑TT‑T‑T‑GCCCCCGGTTTTCCCCGCCCCACCCCCAAACAAAA‑GACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct < 6:82942/139‑1 (MQ=255) cGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGC‑AGC‑CTT‑TT‑T‑A‑GCAGCCCGATTTCTCAGCCGCAGCCCGCAAGAAAA‑AACGGCCCAGTACTATAAACGCAAACTAACCCCTCTAGCAAAACTAAAGAct > 8:393387/1‑138 (MQ=255) cGTGTCATACAAAAACACCCCCCCGCGTCTTTTTGGCAGGC‑CCC‑CTT‑TT‑T‑C‑CCCGCCCGGTTGCACCCCCGCCGCCCGCAATAAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct < 2:35259/139‑1 (MQ=255) aaaaCACCCCCCGGCTTCTTTTGGCCCGGC‑CGG‑T‑T‑TT‑TAA‑GCCCCCCGTTTACACCGCCCCCCCCCCCAACAAAC‑CACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGAcc < 1:121942/139‑1 (MQ=255) cTCCGCTTCTTTTTGGCAGGC‑CGC‑CTT‑TT‑T‑A‑GCAGCCCGTTTTCTAAGCCGCAGCCCGCCAGAAAA‑GACGGCCGAGTTGTTTTGTCGCAAACATGCGGCGCGAGCAAAACTCATTCATCTATGAGATACCGGGGaaa > 6:262288/1‑137 (MQ=255) cTCCGCTTCTTTTTGGCAGGC‑AGC‑CTT‑TT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAA‑GCCGGCCGAGTTCGTTTCTCGCCAACTAGCGGCTCTAGCAAAACTCAGGACTCTATGATAGACCGTGGaaa > 7:165254/1‑137 (MQ=255) cTCCGCTTCTTTTTGGCAGGC‑AGC‑CTT‑TT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAA‑GACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGaaa > 1:358022/1‑137 (MQ=255) cTCCGCTTCTTTTTGGCAGGC‑AGC‑CTT‑TT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAA‑GACGGCCGATTTAATTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTATATGATAGACCGTGGaaa > 3:318368/1‑137 (MQ=255) gc‑agC‑CTT‑TT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAA‑GACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAatat > 3:57848/1‑139 (MQ=255) t‑tt‑t‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAA‑GACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCGAGCCAAACACAGGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACCATATGattca > 2:269257/1‑134 (MQ=255) agcCCGTTTTCTCAGCCGCAGCCCGCAAGAAAA‑GACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATTTGAGTGAAAAAAcc > 5:380102/1‑138 (MQ=255) agcCCGTTTTCTCAGCCGCAGCCCGCAAGAAAA‑GACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAAcc > 8:312038/1‑138 (MQ=255) agcCCGTTTTCTCAGCCGCAGCCCGCAAGAAAA‑GACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCGATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAAcc > 1:337899/1‑138 (MQ=255) agcCCGTTTTCTCAGCCGCAGCCCGCAAGAAAA‑GACGGCCGAGTTCTTTTCTAGCAAACTAGCGGCTCTAGCAAAACTCCTGACTCTATGTTAGACAGTGGAAAGAGAATTCTTTTTGACAAAATGAGTGTAAAAAcc > 5:40408/1‑138 (MQ=255) cccGTTTTCTCAGCCGCAGCCCGCAAGAAAA‑GACGGCCGATTTCGTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAAc > 4:215313/1‑139 (MQ=255) | CGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGC‑AGC‑C‑T‑TT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCACGGGAAAA‑GACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAAC > CP006881/3725109‑3725353 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |