Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	1,158,754	A→G	T229T (ACA→ACG)	U712_05745 →	N‑acetyl‑gamma‑glutamyl‑phosphate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	1,158,754	0	A	G	100.0%	178.1 / NA	47	T229T (ACA→ACG)	U712_05745	N‑acetyl‑gamma‑glutamyl‑phosphate reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/29);  total (18/29)

AGCATCCATGGGAACTCATTTTTCTGAGCTGAACGACAATTTTAAAATTTATAAAGTCAATGAACATCAGCACACGCCGGAAATTGAGCAGGCGCTGAATGAATGGCAGCCAGGTCTCGGGCCCATTACATTTTCGGCTCACTTGGTTCCGATGACAAGGGGCATCATGGCGACGATGTATACCAGATTAACCTGTGACCTAACCGCAGATGACCTGCATGATTTATATTCGGAATTTTACCAAGATTCATATTTTGTGAGAG  >  CP006881/1158625‑1158887                                                                                                                                  |                                                                                                                                      aGCATCCATGGGAACTCATTTTTCTGAGCTGAACGACAATTTTAAAATTTATAAAGTCAATGAACATCAGCACACGCCGGAAATTGAGCAGGCGCTGAATGAATGGCAGCCAGGTCTCGGGCCCATTACGTTTTCGGCt                                                                                                                              >  2:217589/1‑139 (MQ=255)    aTCCATGGGAACTCATTTTTCTGAGCTGAACGACAATTTTAAAATTTATAAAGTCAATGAACATCAGCACACGCCGGAAATTGAGCAGGCGCTGAATGAATGGCAGCCAGGTCTCGGGCCCATTACGTTTTCGGCTCAc                                                                                                                           >  7:195252/1‑139 (MQ=255)             aaCTCATTTTTCTGAGCTGAACGACAATTTTAAAATTTATAAAGTCAATGAACATCATCACACGCCGGAATTTGAGCAGGCGCTGAATGAATGGCAGCCAGGTCTCGGGCCCATTACGTTTTCGGCTCACTTGGTTCCg                                                                                                                  <  7:339643/139‑1 (MQ=255)             aaCTCATTTTTCTGAGCTGAACGACAATTTTAAAATTTATAAAGTCAATGAACATCAGCACACGCCGGAAATTGAGCAGGCGCTGAATGAATGGCAGCCAGGTCTCGGGCCCATTACGTTTTCGGCTCACTTGGTTcc                                                                                                                   >  2:94498/1‑138 (MQ=255)             aaCTCATTTTTCTGAGCTGAACGACAATTTTAAAATTTATAAAGTCAATGAACATCAGCACACGCCGGAAATTGAGCAGGCGCTGAATGAATGGCAGCCAGGTCTCGGGCCCATTACGTTTTCGGCTCACTTGGTTCCg                                                                                                                  <  7:22888/139‑1 (MQ=255)              aCTCATTTTTCTGAGCTGAACGACAATTTTAAAATTTATAAAGTCAATGAACATCAGCACACGCCGGAAATTGAGCAGGCGCTGAATGAATGGCAGCCAGGTCTCGGGCCCATTACGTTTTCGGCTCACTTGGTTCCGa                                                                                                                 <  2:10248/139‑1 (MQ=255)               cTCATTTTTCTGTGCTGAACGACAATTTTAAAATTTATAAAGTCAATGAACATCAGCACACGCCGGAAATTGAGCAGGCGCTGAATGAATGGCAGCCAGGTCTCGGGCCCATTACGTTTTCGGCTCACTTGGTTCCGAt                                                                                                                >  5:279009/1‑139 (MQ=255)               cTCATTTTTCTGAGCTGAACGACAATTTTAAAATTTATAAAGTCAATGAACATCAGCACACGCCGGAAATTGAGCAGGCGCTGAATGAATGGCAGCCAGGTCTCGGGCCCATTACGTTTTCGGCTCACTTGGTTCCGAt                                                                                                                <  1:49601/139‑1 (MQ=255)               cTCATTTTTCTGAGCTGAACGACAATTTTAAAATTTATAAAGTCAATGAACATCAGCACACGCCGGAAATTGAGCAGGCGCTGAATGAATGGCAGCCAGGTCTCGGGCCCATTACGTTTTCGGCTCACTTGGTTCCGAt                                                                                                                >  1:242811/1‑139 (MQ=255)               cTCATTTTTCTGAGCTGAACGACAATTTTAAAATTTATAAAGTCAATGAACATCAGCACACGCCGGAAATTGAGCAGGCGCTGAATGAATGGCAGCCAGGTCTCGGGCCCATTACGTTTTCGGCTCACTTGGTTCCGAt                                                                                                                >  3:278214/1‑139 (MQ=255)                  aTTTTTCTGAGCTGAACGACAATTTTAAAATTTATAAAGTCAATGAACATCAGCACACGCCGGAAATTGAGCAGGCGCTGAATGAATGGCAGCCAGGTCTCGGGCCCATTACGTTTTCGGCTCACTTGGTTCCGATGAc                                                                                                             <  3:331524/139‑1 (MQ=255)                      ttCTGTGCTGAACGACAATTTTAAAATTTATAAAGTCAATGAACATCAGCACACGCCGGAAATTGAGCAGGCGCTGAATGAATGGCAGCCAGGTCTCGGGCCCATTACGTTTTCGGCTCACTTGGTTCCGATGACAAg                                                                                                          <  6:279009/138‑1 (MQ=255)                      ttCTGAGCTGAACGACAATTTTAAAATTTATAAAGTCAATGAACATCAGCACACGCCGGAAATTGAGCAGGCGCTGAATGAATGGCAGCCAGGTCTCGGGCCCATTACGTTTTCGGCTCACTTGGTTCCGATGACAAg                                                                                                          <  2:242811/138‑1 (MQ=255)                       tCTGAGCTGAACGACAATTTTAAAATTTATAAAGTCAATGAACATCAGCACACGCCGGAAATTGAGCAGGCGCTGAATGAATGGCAGCCAGGTCTCGGGCCCATTACGTTTTCGGCTCACTTGGTTCCGATGACAAggg                                                                                                        >  1:37759/1‑139 (MQ=255)                                aaCGACAATTTTAAAATTTATAAAGTCAATGAACATCAGCACACGCCGGAAATTGAGCAGGCGCTGAATGAATGGCAGCCAGGTCTCGGGCCCATTACGTTTTCGGCTCACTTGGTTCCGATGACAAGGGGCATCATgg                                                                                               >  7:240361/1‑139 (MQ=255)                                   gACAATTTTAAAATTTATAAAGTCAATGAACATCAGCACACGCCGGAAATTGAGCAGGCGCTGAATGAATGGCAGCCAGGTCTCGGGCCCATTACGTTTTCGTCTCACTTGGTTCCGATGACAAGGGGCATCATGGcga                                                                                            <  7:376384/139‑1 (MQ=255)                                   gACAATTTTAAAATTTATAAAGTCAATGAACATCAGCACACGCCGGAAATTGAGCAGGCGCTGAATGAATGGCAGCCAGGTCTCGGGCCCATTACGTTTTCGGCTCACTTGGTTCCGATGACAAGGGGCATCATGGcga                                                                                            <  4:278214/139‑1 (MQ=255)                                   gACAATTTTAAAATTTATAAAGTCAATGAACATCAGCACACGCCGGAAATTGAGCAGGCGCTGAATGAATGGCAGCCAGGTCTCGGGCCCATTACGTTTTCGGCTCACTTGGTTCCGATGACAAGGGGCATCATGGcga                                                                                            <  2:37759/139‑1 (MQ=255)                                   gACAATTTTAAAATTTATAAAGTCAATGAACATCAGCACACGCCGGAAATTGAGCAGGCGCTGAATGAATGGCAGCCAGGTCTCGGGCCCATTACGTTTTCGGCTCACTTGGTTCCGATGACAAGGGGCATCATGGcga                                                                                            <  7:294887/139‑1 (MQ=255)                                     cAATTTTAAAATTTATAAAGTCAATGAACATCATCACACTCCGGAAATTGAGCAGGCTCTGAATGAATGGCTGCCAGGTCTAGGGCCAATTTCGTTTTCGGCTCACTTGGTTCCGATGACAAGGGGCATCTTGGcgacg                                                                                          <  7:274543/139‑1 (MQ=255)                                       aTTTTAAAATTTATAAAGTCAATGAACATCAGCACACGCCGGAAATTGAGCAGGCGCTGAATGAATGGCAGCCAGGTCTCGGGCCCATTACGTTTTCGGCTCACTTGGTTCCGATGACAAGGGGCATCATGGCGACGAt                                                                                        <  1:217589/139‑1 (MQ=255)                                            aaatTTTATAAAGTCAATGAACATCAGCACACGCCGGAAATTGAGCAGGCGCTGAATGAATGGCAGCCAGGTCTCGGGCCCATTACGTTTTCGGCTCACTTGGTTCCGATGACAAGGGGCATCATGGCGACGATGtata                                                                                   <  5:227831/139‑1 (MQ=255)                                             aaaTTTATAAAGTCAATGAACATCAGCACACGCCGGAAATTGAGCAGGCGCTGAATGAATGGCAGCCAGGTCTCGGGCCCATTACGTTTTCGGCTCACTTGGTTCCGATGACAAGGGGCATCATGGCGACGATGtata                                                                                   <  5:206618/138‑1 (MQ=255)                                             aaaTTTATAAAGTCAATGAACATCAGCACACGCCGGAAATTGAGCAGGCGCTGAATGAATGGCAGCCAGGTCTCGGGCCCATTACGTTTTCGGCTCACTTGGTTCCGATGACAAGGGGCATCATGGCGACGATGtata                                                                                   <  8:195252/138‑1 (MQ=255)                                             aaaTTTATAAAGTAAATGAACATCAGCACACGCCGGAAATTGAGCAGGCGCTGAATGAATGGCAGCCAGGTCTCGGGCCCATTACGTTTTCGGCTCACTTGGTTCCGATGACAAGGGGCATCATGGCGACGATGtata                                                                                   <  1:94498/138‑1 (MQ=255)                                                    taAAGTCAATGAACATCAGCACACGCCGGAAATTGAGCAGGGGCTGAATGAATGGCAGCCAGGTCTCGGGGACATTACGTTTTCGGCTCACTTGGTTCCGATGACAAGGGGCATCATGGCGACGATGTATACCAGAtt                                                                            <  5:241004/138‑1 (MQ=255)                                                    taAAGTCAATGAACATCAGCACACGCCGGAAATTGAGCAGGCGCTGAATGAATGGCAGCCAGGTCTCGGGCCCATTACGTTTTCGGCTCACTTGGTTCCGATGACAAGGGGCATCATGGCGACGATGTATACCAGAtt                                                                            <  5:156345/138‑1 (MQ=255)                                                                  aTCAGCACACGCCGGAAATTGAGCAGGCGCTGAATGAATGGCAGCCAGGGCTCGGGCCCATTACGTTTTCGGCTCACTTGGTTCCGATGACAAGGGGCATCATGGCGACGATGTATACCAGATTAACCTGTGACCTAAc                                                             <  3:348889/139‑1 (MQ=255)                                                                     aGCACACGCCGGAAATTGAGCAGGCGCTGAATGAATGGCAGCCAGGTCTCGGGCCCATTACGTTTTCGGCTCACTTGGTTCCGATGACAAGGGGCATCATGGCGACGATGTATACCAGATTAACCTGTGACCTAACCg                                                           <  7:337615/138‑1 (MQ=255)                                                                         cacGCCGGAAATTGAGCAGGCGCTGAATGAATGGCAGCCAGGTCTCGGGCCCATTACGTTTTCGGCTCACTTGGTTCCGATGACAAGGGGCATCATGGCGACGATGTATACCAGATTAACCTGTGACCTaa                                                              >  4:356768/1‑131 (MQ=255)                                                                         cacGCCGGAAATTGAGCAGGCGCTGAATGAATGGCAGCCAGGTCTCGGGCCCATTACGTTTTCGGCTCACTTGGTTCCGATGACAAGGGGCATCATGGCGACGATGTATACCAGATTAACCTGTGACCTaa                                                              <  3:356768/131‑1 (MQ=255)                                                                                 aaaTTGAGCAGGCGCTGAATGAATGGCAGCCAGGTCTCGGGCCCATTACGTTTTCGGCTCACTTGGTTCCGATGACAAGGGGCATCATGGCGACGATGTATACCAGATTaa                                                                          <  5:104362/111‑1 (MQ=255)                                                                                 aaaTTGAGCAGGCGCTGAATGAATGGCAGCCAGGTCTCGGGCCCATTACGTTTTCGGCTCACTTGGTTCCGATGACAAGGGGCATCATGGCGACGATGTATACCAGATTaa                                                                          >  6:104362/1‑111 (MQ=255)                                                                                 aaaTTGAGCAGGCGCTGAATGAATGGCAGCCAGGTCTCGGGCCCATTACGTTTTCGGCTCACTTGGTTCCGATGACAAGGGGCATCATGGCGACGATGTATACCAGATTAACCTGTGACCTAACCGCAGATGACCTGCa                                              >  6:40466/1‑139 (MQ=255)                                                                                  aaTTGAGCAGGCGCTGAATGAATGGCAGCCAGGTCTCGGGCCCATTACGTTTTCGGCTCACTTGGTTCCGATGACAAGGGGCATCATGGCGACGATGTATACCAGATTAACCTGTGACCTAACCGCAGATGACCTGCAt                                             <  2:254463/139‑1 (MQ=255)                                                                                            gcgcTGATTGAATGGCAGGCAGGTCTCGGGCCCATTACGTTTTCGGCTCACTTGGTTCCGATGACAAGGGGCATCATGGCGACGATGTATACCAGATTAACCTGTGACCTAACCGCAGATGACCTGCATGATTTATAtt                                   <  5:381989/139‑1 (MQ=255)                                                                                            gcgcTGAATGAATGGCAGCCAGGTCTCGGGCCCATTACGTTTTCGGCTCACTTGGTTCCg                                                                                                                  >  6:126947/1‑60 (MQ=255)                                                                                            gcgcTGAATGAATGGCAGCCAGGTCTCGGGCCCATTACGTTTTCGGCTCACTTGGTTCCg                                                                                                                  <  5:126947/60‑1 (MQ=255)                                                                                            gcgcTGAATGAATGGCAGCCAGGTCTCGGGCCCATTACGTTTTCGGCTCACTTGGTTCCGATGACAAGGGGCATCATGGCGACGATGTATACCAGATTAACCTGTGACCTAACCGCAGATGACCTGCATGATTTATAtt                                   <  7:61604/139‑1 (MQ=255)                                                                                                 gaatgaatGGCAGCCAGGTCTCGGGCCCATTACGTTTTCGGCTCACTTGGTTCCGATGACAAGGGGCATCATGGCGACGATGTATACCAGATTAACCTGTGACCTAACCGCAGATGACCTGCATGATTTATATTCGGaa                              >  5:289847/1‑139 (MQ=255)                                                                                                      aatgGCAGCCAGGTCTCGGGCCCATTACGTTTTCGGCTCACTTGGTTCCGATGACAAGGGGCATCATGGCGACGATGTATACCAGATTAACCTGTGACCTAACCGCAGATGACCTGCATGATTTATATTCGGAATTTTa                         >  6:115350/1‑139 (MQ=255)                                                                                                      aatgGCAGCCAGGTCTCGGGCCCATTACGTTTTCGGCTCACTTGGTTCCGATGACAAGGGGCATCATGGCGACGATGTATACCAGATTAACCTGTGACCTAAACGCAGATGACCTGCATGATTTATATTCGGAATTTTa                         >  8:49425/1‑139 (MQ=255)                                                                                                        tgGCAGCCAGGTCTCGGGACCATTACGTTTTCGGCTCACTTGGTTCCGATGACAAGGGGCATCATGGCGACGATGTATACCAGATTAACCTGTGACCTAACCGCAGATGACCTGCATGATTTATATTCGGAATTTTAcc                       <  8:240361/139‑1 (MQ=255)                                                                                                              ccAGGTCTCGGGCCCATTACGTTTTCGGCTCACTTGGTTCCGATGACAAGGGGCATCATGGCGACGATGTATACCAGATTAACCTGTGACCTAACCGCAGATGACCTGCATGATTTATATTCGGAATTTTACCAAGAtt                 <  5:40466/139‑1 (MQ=255)                                                                                                                            cATTACGTTTTCGGCTCACTTGGTTCCGATGACAAGGGGCATCATGGAGACGATGTATACCAGATTAACCTGTGACCTAACCGCAGATGACCTGCATGATTTATATTCGGAATTTTACCAAGATTCATATTTTGTga     >  2:257272/1‑137 (MQ=255)                                                                                                                             aTTACGTTTTCGGCTCACTTGGTTCCGATGACAAGGGGCATCATGGCGACGATGAATACAAGATTAACATTTGACCTAACCGCAGATGACCTGCATGATTTATATTCGAAATTTTACCAAGATTCATATTTTGTGagag  >  1:365045/1‑139 (MQ=255)                                                                                                                             aTTACGTGTTCGGCTCACTTGGTTCCGATGACAAGCGGCATCATGGCGAAGATGTATACAAGATAACCCTGTGACCTAACCGCAGATGACGTGCATGATTAATATTCGGATTTTAACCATCATTCTTATTTTGTGagag  >  2:248368/1‑139 (MQ=255)                                                                                                                                  |                                                                                                                                      AGCATCCATGGGAACTCATTTTTCTGAGCTGAACGACAATTTTAAAATTTATAAAGTCAATGAACATCAGCACACGCCGGAAATTGAGCAGGCGCTGAATGAATGGCAGCCAGGTCTCGGGCCCATTACATTTTCGGCTCACTTGGTTCCGATGACAAGGGGCATCATGGCGACGATGTATACCAGATTAACCTGTGACCTAACCGCAGATGACCTGCATGATTTATATTCGGAATTTTACCAAGATTCATATTTTGTGAGAG  >  CP006881/1158625‑1158887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑