Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,724,467	T→C	K120K (AAA→AAG)	U712_19200 ←	SacPA operon antiterminator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,724,467	0	T	C	100.0%	315.6 / NA	86	K120K (AAA→AAG)	U712_19200	SacPA operon antiterminator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (43/43);  total (43/43)

CTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGAAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCGTATTCTTTTTTATAGAGCGCTTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCAGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCCCCTTCCGCATAGCTG  >  CP006881/3724332‑3724602                                                                                                                                        |                                                                                                                                        ctcCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCctt                                                                                                                                       >  3:182616/1‑138 (MQ=255) ctcCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCctt                                                                                                                                       >  6:146569/1‑138 (MQ=255) ctcCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCctt                                                                                                                                       >  7:291035/1‑138 (MQ=255) ctcCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCAGATCGCCCACAGGCCGCTCTCATATTCTTTTTTATAGAGCGCctt                                                                                                                                       >  7:391878/1‑138 (MQ=255) ctcCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATATTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCTATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCctt                                                                                                                                       >  3:170615/1‑138 (MQ=255)    cGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTAtc                                                                                                                                   >  3:396846/1‑139 (MQ=255)          catcTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATg                                                                                                                              <  3:154625/138‑1 (MQ=255)           atcTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATgca                                                                                                                            <  8:97559/139‑1 (MQ=255)           atcTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATgca                                                                                                                            <  5:270167/139‑1 (MQ=255)           atcTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATgca                                                                                                                            <  5:320636/139‑1 (MQ=255)                cGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAAt                                                                                                                       >  8:385852/1‑139 (MQ=255)                      aTGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCATCAATTTATTc                                                                                                                 >  1:191761/1‑139 (MQ=255)                         gATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTg                                                                                                               <  7:258538/138‑1 (MQ=255)                         gATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGc                                                                                                              <  4:44123/139‑1 (MQ=255)                          aTGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGca                                                                                                             >  3:82293/1‑139 (MQ=255)                             tgAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACaa                                                                                                          <  3:33164/139‑1 (MQ=255)                             tgAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGACCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACaa                                                                                                          >  4:160727/1‑139 (MQ=255)                               aaGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAag                                                                                                         >  1:392504/1‑138 (MQ=255)                                   gCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGcc                                                                                                    <  7:385852/139‑1 (MQ=255)                                    cAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGcc                                                                                                    >  2:188216/1‑138 (MQ=255)                                    cAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGcc                                                                                                    >  4:314541/1‑138 (MQ=255)                                    cAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGcc                                                                                                    >  4:4816/1‑138 (MQ=255)                                    cAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGcc                                                                                                    >  2:21922/1‑138 (MQ=255)                                    cAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGAGCCTTTATCTCATGAAGCAATATATTCTGCACAACCAGcc                                                                                                    >  5:44763/1‑138 (MQ=255)                                       tataACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCAtt                                                                                                >  3:391183/1‑139 (MQ=255)                                               gCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATcc                                                                                        >  6:37073/1‑139 (MQ=255)                                                 ttCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCtt                                                                                      >  6:59067/1‑139 (MQ=255)                                                 ttCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCtt                                                                                      >  2:35828/1‑139 (MQ=255)                                                         cAGGCAGAGCCACGCCCAATGTCTCTGTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCCGCCCAGTTTGAATCCTTTCGATCGc                                                                              >  8:158647/1‑139 (MQ=255)                                                          aGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCa                                                                             <  3:160727/139‑1 (MQ=255)                                                                agaCACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGa                                                                        >  8:101066/1‑138 (MQ=255)                                                                agaCACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGa                                                                        >  4:9125/1‑138 (MQ=255)                                                                      gCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATgg                                                                 >  1:24065/1‑139 (MQ=255)                                                                      gCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATgg                                                                 >  5:271638/1‑139 (MQ=255)                                                                      gCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATgg                                                                 >  7:133780/1‑139 (MQ=255)                                                                              tctcTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATCTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAg                                                         <  1:188216/139‑1 (MQ=255)                                                                                tctTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATgca                                                                                                                            >  8:145360/1‑70 (MQ=255)                                                                                tctTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATgca                                                                                                                            <  7:145360/70‑1 (MQ=255)                                                                                  ttttACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCTATTTATTCTGCCCAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAACgcgc                                                      >  4:156051/1‑138 (MQ=255)                                                                                  ttttACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgcgc                                                      >  1:41737/1‑138 (MQ=255)                                                                                  ttttACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgcgc                                                      >  7:20854/1‑138 (MQ=255)                                                                                  ttttACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgcg                                                       <  2:41737/137‑1 (MQ=255)                                                                                  ttttACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgcg                                                       <  1:21922/137‑1 (MQ=255)                                                                                  ttttACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgcg                                                       <  3:225067/137‑1 (MQ=255)                                                                                  ttttACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgcg                                                       <  5:146569/137‑1 (MQ=255)                                                                                  ttttACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgcg                                                       <  4:82293/137‑1 (MQ=255)                                                                                  ttttACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgcg                                                       <  4:121131/137‑1 (MQ=255)                                                                                    ttACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGcgcg                                                     <  2:45073/137‑1 (MQ=255)                                                                                    ttACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGa                                                    >  7:336985/1‑138 (MQ=255)                                                                                     tACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGat                                                   <  8:20854/138‑1 (MQ=255)                                                                                          gTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAAt                                             >  3:280477/1‑139 (MQ=255)                                                                                          gTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAAt                                             >  6:13575/1‑139 (MQ=255)                                                                                          gTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAAt                                             >  6:127411/1‑139 (MQ=255)                                                                                          gTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAAt                                             >  8:233907/1‑139 (MQ=255)                                                                                            ccGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAAtgt                                           <  2:385522/139‑1 (MQ=255)                                                                                            ccGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAAtgt                                           >  6:314933/1‑139 (MQ=255)                                                                                             cGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAAtgt                                           <  1:35828/138‑1 (MQ=255)                                                                                              gATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGa                                         <  2:24065/139‑1 (MQ=255)                                                                                                  gCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCg                                      <  6:205740/138‑1 (MQ=255)                                                                                                  gCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCg                                      <  8:291035/138‑1 (MQ=255)                                                                                                       caGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATg                                                                                                                              >  2:380045/1‑45 (MQ=255)                                                                                                       caGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATg                                                                                                                              <  1:380045/45‑1 (MQ=255)                                                                                                              aTCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGATTCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGcgccg                         >  1:47941/1‑139 (MQ=255)                                                                                                              aTCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGa                                         <  4:356524/123‑1 (MQ=255)                                                                                                              aTCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGa                                         >  3:356524/1‑123 (MQ=255)                                                                                                               tctcATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGAGGcgccgc                        >  8:50333/1‑139 (MQ=255)                                                                                                                   atATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGc                    <  7:233907/138‑1 (MQ=255)                                                                                                                   atATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGc                    <  4:222250/138‑1 (MQ=255)                                                                                                                   atATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCACATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCcgcg                       <  3:9125/135‑1 (MQ=255)                                                                                                                      ttCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTc                  <  6:271638/138‑1 (MQ=255)                                                                                                                      ttCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTc                  <  4:280477/138‑1 (MQ=255)                                                                                                                         ttttttATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCt              >  2:217421/1‑139 (MQ=255)                                                                                                                         ttttttATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCt              >  7:261132/1‑139 (MQ=255)                                                                                                                           ttttATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCa                                                                             <  4:329218/74‑1 (MQ=255)                                                                                                                           ttttATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCa                                                                             >  3:329218/1‑74 (MQ=255)                                                                                                                           ttttATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTc            >  5:110884/1‑139 (MQ=255)                                                                                                                           ttttATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCGCCTTc            >  4:238816/1‑139 (MQ=255)                                                                                                                           ttttATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATACTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTctcc               <  3:79508/136‑1 (MQ=255)                                                                                                                              tataGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCg          <  6:110884/138‑1 (MQ=255)                                                                                                                                taGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCa        <  5:59067/138‑1 (MQ=255)                                                                                                                                taGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCa        <  5:37073/138‑1 (MQ=255)                                                                                                                                taGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCa        <  1:217421/138‑1 (MQ=255)                                                                                                                                taGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCa        <  3:314541/138‑1 (MQ=255)                                                                                                                                taGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCAt       <  3:4816/139‑1 (MQ=255)                                                                                                                                taGAGCGACTTTATCTCATGCAGCAATTTAATCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCa        <  6:44763/138‑1 (MQ=255)                                                                                                                                  gagCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAg     <  2:392504/139‑1 (MQ=255)                                                                                                                                   agCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCg          <  4:398494/133‑1 (MQ=255)                                                                                                                                   agCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCg          >  3:398494/1‑133 (MQ=255)                                                                                                                                     cgcCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTc                  >  3:301175/1‑123 (MQ=255)                                                                                                                                     cgcCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTc                  <  4:301175/123‑1 (MQ=255)                                                                                                                                     cgcCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTGCTTCCGCATAGCtg  >  1:164288/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                        CTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGAAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCGTATTCTTTTTTATAGAGCGCTTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCAGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCCCCTTCCGCATAGCTG  >  CP006881/3724332‑3724602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑