Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,723,067 1 bp→AT intergenic (‑181/‑120) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,723,0661.A92.0% 374.0 / 22.1 112intergenic (‑180/‑121)U712_19190/U712_19195PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base . (4/5);  new base A (36/67);  total (40/72)
Fisher's exact test for biased strand distribution p-value = 7.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0068813,723,0670GT92.9% 360.9 / 6.4 112intergenic (‑181/‑120)U712_19190/U712_19195PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (35/69);  minor base A (5/3);  total (40/72)
Fisher's exact test for biased strand distribution p-value = 1.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

AACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAAC‑T‑GT‑T‑TTTTAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAGGAAAAAACAATGGAAACTCAAGCA  >  CP006881/3722933‑3723201
                                                                                                                                        ||                                                                                                                                       
caCGCATAAAGGACACAGCGATCCTTTATCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑T‑At‑t‑tt                                                                                                                                    <  2:259726/138‑1 (MQ=255)
  cGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑T‑At‑t‑tttt                                                                                                                                  <  5:117948/139‑1 (MQ=255)
  cGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑T‑At‑t‑tttt                                                                                                                                  >  3:119639/1‑139 (MQ=255)
          ggACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTaaa                                                                                                                           <  8:48532/139‑1 (MQ=255)
          ggACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTaaa                                                                                                                           <  1:246733/139‑1 (MQ=255)
          ggACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTaaa                                                                                                                           <  8:47694/139‑1 (MQ=255)
          ggACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTaaa                                                                                                                           <  5:66730/139‑1 (MQ=255)
          ggACACAGAGATCCTTTATCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGGCACAATCCCGCTTATTCAGATTTAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTaaa                                                                                                                           <  8:326728/139‑1 (MQ=255)
           gACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑T‑At‑t‑tttt                                                                                                                                  >  4:232259/1‑130 (MQ=255)
           gACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑T‑At‑t‑tttt                                                                                                                                  <  3:232259/130‑1 (MQ=255)
             cacaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAg                                                                                                                        >  8:391122/1‑139 (MQ=255)
              acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATTGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAAAAGt                                                                                                                       >  7:98618/1‑139 (MQ=255)
              acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGt                                                                                                                       <  6:35596/139‑1 (MQ=255)
              acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGt                                                                                                                       >  4:357777/1‑139 (MQ=255)
              acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGt                                                                                                                       <  2:222008/139‑1 (MQ=255)
              acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATACCGCTTATTCAGATTAAGAAAACGATTTCATCATAAATCAAGATAGCGTTAACGTCAAC‑T‑AT‑TATTTTAGATAAATAGt                                                                                                                       >  6:10203/1‑139 (MQ=255)
               caGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTAGCAGTCACAATCACGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATATCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGtt                                                                                                                      <  7:329409/139‑1 (MQ=255)
                aGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGttt                                                                                                                     >  6:57346/1‑139 (MQ=255)
                aGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGttt                                                                                                                     >  7:215182/1‑139 (MQ=255)
                     tCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATa                                                                                                                 >  5:230879/1‑138 (MQ=255)
                          tttCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAAcat                                                                                                           >  3:334759/1‑137 (MQ=255)
                           ttCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAAcatg                                                                                                          >  1:279589/1‑136 (MQ=255)
                            tCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTGAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAAcatg                                                                                                          <  6:296005/138‑4 (MQ=255)
                                                  ttGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTaaaaatt                                                                                    >  4:198349/1‑136 (MQ=255)
                                                    gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCTTCAAC‑T‑ATAT‑TTTAAGTTAAATAGTTTGATATAACATCTAGACAAAAATTCTTataaataaat                                                                                 >  2:122545/1‑134 (MQ=255)
                                                    gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaa                                                                                  <  3:73173/138‑2 (MQ=255)
                                                        gCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTTATtgtg                                                                             <  5:87340/139‑1 (MQ=255)
                                                         cTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATTTAGACAAAAATTCGTAAAAATTAATtgtg                                                                             >  6:323076/1‑138 (MQ=255)
                                                         cTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATtgtg                                                                             <  1:220737/138‑1 (MQ=255)
                                                            tACCAGTCACAATCCCGCTTATTCATATTAATAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTT‑TT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaa                                                                          <  8:253551/138‑1 (MQ=255)
                                                              ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                        <  5:350801/138‑3 (MQ=255)
                                                              ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                        <  5:283094/138‑3 (MQ=255)
                                                              ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                        <  7:16647/138‑3 (MQ=255)
                                                              ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                        <  5:323076/138‑3 (MQ=255)
                                                              ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                        <  1:245178/138‑3 (MQ=255)
                                                              ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                        <  3:272136/138‑3 (MQ=255)
                                                              ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATAc                                                                       >  7:61552/1‑139 (MQ=255)
                                                              ccAGTCACAATCCCGCTTATTCAGATTAAGAATAAGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                        <  8:101989/138‑3 (MQ=255)
                                                               cAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACt                                                                      <  7:32828/139‑1 (MQ=255)
                                                                  tCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                                   <  5:285547/139‑1 (MQ=255)
                                                                  tCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                                   <  4:372745/139‑1 (MQ=255)
                                                                   cacaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTaaa                                                                                                                           <  1:81147/82‑1 (MQ=255)
                                                                   cacaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTaaa                                                                                                                           >  2:81147/1‑82 (MQ=255)
                                                                       aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  7:355587/139‑1 (MQ=255)
                                                                       aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  2:293346/139‑1 (MQ=255)
                                                                       aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  2:153539/139‑1 (MQ=255)
                                                                       aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  2:115681/139‑1 (MQ=255)
                                                                       aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  4:334759/139‑1 (MQ=255)
                                                                       aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  4:119639/139‑1 (MQ=255)
                                                                       aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  7:35482/139‑1 (MQ=255)
                                                                       aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  3:79694/139‑1 (MQ=255)
                                                                       aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  8:215182/139‑1 (MQ=255)
                                                                       aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  1:377698/139‑1 (MQ=255)
                                                                       aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  1:276549/139‑1 (MQ=255)
                                                                       aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  8:379124/139‑1 (MQ=255)
                                                                       aTCCCGCCTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGGTTTCGTCAAC‑TATT‑T‑TTTTAGTTAACTAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  4:23500/139‑1 (MQ=255)
                                                                       aTCCAGCTTAATCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAATAATTAATTGTGAAATACTTCACAata                                                              <  4:45016/139‑1 (MQ=255)
                                                                          ccGCTTATTCAGATTAAGAATACGCTTTCATCATATATCATGATAGCGTTTTCGTCAACTT‑TT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAATATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                                            <  4:194849/138‑1 (MQ=255)
                                                                          ccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                                            <  1:327877/138‑1 (MQ=255)
                                                                          ccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                                            <  3:79510/138‑1 (MQ=255)
                                                                          ccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                                            <  4:36304/138‑1 (MQ=255)
                                                                            gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                         <  1:191647/139‑1 (MQ=255)
                                                                            gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                         <  3:215728/139‑1 (MQ=255)
                                                                            gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                         <  4:125384/139‑1 (MQ=255)
                                                                            gCTTATTCAGATTAAGAATACGCTTTCATCATAAAACATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                         <  1:52480/139‑1 (MQ=255)
                                                                             cTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGc                                                        >  1:129866/1‑139 (MQ=255)
                                                                             cTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGc                                                        >  7:242452/1‑139 (MQ=255)
                                                                                aTTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGc                                                        >  4:24137/1‑136 (MQ=255)
                                                                                aTTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGc                                                        <  3:24137/136‑1 (MQ=255)
                                                                                  tCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATAc                                                   >  1:40057/1‑139 (MQ=255)
                                                                                    aGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTa                                                 >  8:259914/1‑139 (MQ=255)
                                                                                      aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACaa                                                                >  2:323628/1‑122 (MQ=255)
                                                                                      aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACaa                                                                <  1:323628/122‑1 (MQ=255)
                                                                                      aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  5:2159/124‑1 (MQ=255)
                                                                                      aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  1:376465/124‑1 (MQ=255)
                                                                                      aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              >  2:376465/1‑124 (MQ=255)
                                                                                      aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  8:288118/124‑1 (MQ=255)
                                                                                      aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              >  7:288118/1‑124 (MQ=255)
                                                                                      aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              >  6:2159/1‑124 (MQ=255)
                                                                                         aagaATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCt                                             <  4:124161/137‑1 (MQ=255)
                                                                                         aagaATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCt                                             <  2:40057/137‑1 (MQ=255)
                                                                                              tACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                         >  6:100947/1‑121 (MQ=255)
                                                                                              tACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                         <  5:100947/121‑1 (MQ=255)
                                                                                                ctcTTTCATCATAAATCATGATAGCGTTTTCGTCAAA‑TAAT‑T‑TTTTAATTAAATAATTTGAAATAAAATGTATAAAAAAATTCGTAAAAATTAATTGTGAAATAATTAAAAATATCTTGCTATACTATGATAAATCAt                                      >  6:136429/3‑138 (MQ=255)
                                                                                                cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              >  7:281947/1‑114 (MQ=255)
                                                                                                cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCAt                                      >  7:311060/1‑138 (MQ=255)
                                                                                                cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCAt                                      >  1:315963/1‑138 (MQ=255)
                                                                                                cGCTTTCATCATAAACCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCAt                                      >  1:352140/1‑138 (MQ=255)
                                                                                                      catcatAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              >  3:289007/1‑108 (MQ=255)
                                                                                                      catcatAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  4:289007/108‑1 (MQ=255)
                                                                                                        tcatAAATCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCAGAATATCGTGCTATACTATGCTCAATCATGaaagaaag                             <  1:267417/139‑1 (MQ=255)
                                                                                                             aaTCATGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGa                        <  5:57346/139‑1 (MQ=255)
                                                                                                                 aTGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATg                                               <  3:124888/112‑1 (MQ=255)
                                                                                                                 aTGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATg                                               >  4:124888/1‑112 (MQ=255)
                                                                                                                 aTGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaagaaag                             >  6:206467/1‑130 (MQ=255)
                                                                                                                 aTGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaagaaag                             <  5:206467/130‑1 (MQ=255)
                                                                                                                  tGATAGCGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGaaaaga                   >  3:342463/1‑137 (MQ=255)
                                                                                                                        cGTTTTCGTCAAC‑TATT‑T‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACATCACAATATCGTCCTATAGTATGATCAATCATCAAAGAAAGCAGGAAAAGACAAtc              >  8:61380/1‑137 (MQ=255)
                                                                                                                                    actatt‑t‑ttttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCa  <  8:242452/134‑1 (MQ=255)
                                                                                                                                    actatt‑t‑ttttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCa  <  6:202420/134‑1 (MQ=255)
                                                                                                                                        ||                                                                                                                                       
AACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAAC‑T‑GT‑T‑TTTTAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAGGAAAAAACAATGGAAACTCAAGCA  >  CP006881/3722933‑3723201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑