Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,724,208 T→G 100% R207R (AGA→CGA)  U712_19200 ← SacPA operon antiterminator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,724,2080TG100.0% 199.4 / NA 58R207R (AGA→CGA) U712_19200SacPA operon antiterminator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (34/24);  total (34/24)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TATTCACTTTTCAAAAACTCTGCCAGCTCAAGCGCACATTGATAGGCAAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGACTGACTGCGTATCTCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTCTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCAT  >  CP006881/3724072‑3724343
                                                                                                                                        |                                                                                                                                       
tatTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCa                                                                                                                                       <  4:87724/139‑1 (MQ=255)
 atTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCa                                                                                                                                       >  1:90586/1‑138 (MQ=255)
 atTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCa                                                                                                                                       >  7:207201/1‑138 (MQ=255)
 atTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCa                                                                                                                                       >  2:244279/1‑138 (MQ=255)
          tCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGa                                                                                                                              >  7:5015/1‑138 (MQ=255)
           cAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGAc                                                                                                                             <  1:36845/138‑1 (MQ=255)
                aCTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCg                                                                                                                       <  1:206603/139‑1 (MQ=255)
                aCTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCg                                                                                                                       <  2:285929/139‑1 (MQ=255)
                                     aTTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGtttt                                                                                                   >  1:249146/1‑138 (MQ=255)
                                         aTAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATc                                                                                               <  2:30080/138‑1 (MQ=255)
                                                 aTGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACa                                                                                                                            <  4:90908/101‑1 (MQ=255)
                                                 aTGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACa                                                                                                                            >  3:90908/1‑101 (MQ=255)
                                                 aTGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTACATTTGATTCCAACCGGCAGACCGCGTATCGCAAATGCGTGACAAGCCGTTCATAGGAAATGCTGTTTTCATCCACCTTTc                                                                                       >  8:163278/1‑138 (MQ=255)
                                                           tttttGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTa                                                                             <  5:184165/138‑1 (MQ=255)
                                                           ttattGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTACATCCACCTTTCGATTAAAGTAt                                                                            >  6:159154/4‑139 (MQ=255)
                                                                   tGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTAt                                                                    >  6:258096/1‑139 (MQ=255)
                                                                     aaaTAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTAtt                                                                   <  3:216594/138‑1 (MQ=255)
                                                                                ctcctcGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCAtt                                                       >  7:30515/1‑139 (MQ=255)
                                                                                ctcctcGTCCATACGATGGAGCGCTTCATTTGATTCAAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCAtt                                                       >  2:88462/1‑139 (MQ=255)
                                                                                     cGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTAGCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATGTTCTCTATCATTTCttt                                                  >  8:216547/1‑139 (MQ=255)
                                                                                       tCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGa                                                >  4:79991/1‑139 (MQ=255)
                                                                                          aTACGATGGAGCGCTTCATTTGATTCTAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCa                                             <  1:88462/139‑1 (MQ=255)
                                                                                          aTACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGGGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCa                                             <  8:60990/139‑1 (MQ=255)
                                                                                           tACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCAt                                            >  6:200308/1‑139 (MQ=255)
                                                                                           tACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCAt                                            >  3:2584/1‑139 (MQ=255)
                                                                                           tACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCAt                                            >  6:116691/1‑139 (MQ=255)
                                                                                           tACGATGGAGCGCTTCATTTGACTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATGATTTATTTGATCAt                                            >  4:149030/1‑139 (MQ=255)
                                                                                            aCGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCGACCTTTCGATTAAAGTATTGTTTTTTTTTCTAAATCATTTTTTTGATCaag                                           >  2:270332/1‑137 (MQ=255)
                                                                                             cGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATgg                                          <  2:161610/139‑1 (MQ=255)
                                                                                                tGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGAc                                                                                                                             <  7:1406/53‑1 (MQ=255)
                                                                                                tGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGAc                                                                                                                             >  8:1406/1‑53 (MQ=255)
                                                                                                tGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTTTTTTATCGATACTTTCTTTGTTCATGGTCg                                       >  2:133723/1‑139 (MQ=255)
                                                                                                tGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCg                                       >  6:278499/1‑139 (MQ=255)
                                                                                                tGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCg                                       >  5:175733/1‑139 (MQ=255)
                                                                                                tGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCg                                       >  8:134676/1‑139 (MQ=255)
                                                                                                  gAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTa                                     <  5:234252/139‑1 (MQ=255)
                                                                                                   aGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATc                                                                                               <  1:64951/80‑1 (MQ=255)
                                                                                                   aGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATc                                                                                               >  2:64951/1‑80 (MQ=255)
                                                                                                   aGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTAt                                    >  4:55311/1‑139 (MQ=255)
                                                                                                     cgcTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGc                                  >  2:165716/1‑139 (MQ=255)
                                                                                                       cTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCtt                                <  4:2584/139‑1 (MQ=255)
                                                                                                       cTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCtt                                <  7:134676/139‑1 (MQ=255)
                                                                                                        ttCATTTGATTCCATCCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTAATCCCCCTTTCGAATAAAGTATTGTTTTATTTTCACTATCATTTCTTTTATCTTGGTCGTATGCtt                                >  3:104049/1‑138 (MQ=255)
                                                                                                        ttCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCtt                                >  7:283475/1‑138 (MQ=255)
                                                                                                          cATTTGATTCCAACCGGCTGACCGCTTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATTCGGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTGTGAGAATTGTCGTATGCTTcct                             >  6:43128/1‑137 (MQ=255)
                                                                                                          cATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAg                             >  8:178506/1‑139 (MQ=255)
                                                                                                            tttGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAgcg                           <  5:257960/139‑1 (MQ=255)
                                                                                                            tttGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAgcg                           <  8:5015/139‑1 (MQ=255)
                                                                                                              tGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAgcgc                          <  6:175733/138‑1 (MQ=255)
                                                                                                                aTTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTg                        <  6:135615/138‑1 (MQ=255)
                                                                                                                 ttCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATACAACTTTCGATTAAAGTCTTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGaa                      <  5:43128/139‑1 (MQ=255)
                                                                                                                    cAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATAc                   <  6:263568/139‑1 (MQ=255)
                                                                                                                      aCCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACAt                 >  5:158897/1‑139 (MQ=255)
                                                                                                                       ccGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATg                <  7:256383/139‑1 (MQ=255)
                                                                                                                       ccGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATg                <  8:283475/139‑1 (MQ=255)
                                                                                                                          gctgaccgCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTATCGATTAAAGTATTGTTTTATTTTCTCTATCATTTATTTGATCATGGTCTTATGCTTCAGCGCTGAATACATGctc             >  4:68747/2‑139 (MQ=255)
                                                                                                                           ctgaccgCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGctct            >  4:288217/1‑139 (MQ=255)
                                                                                                                                     aTCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCCCCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGcatccat  >  7:125510/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                       
TATTCACTTTTCAAAAACTCTGCCAGCTCAAGCGCACATTGATAGGCAAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGACTGACTGCGTATCTCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTCTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCAT  >  CP006881/3724072‑3724343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑