| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 3,831,557 | G→A | 100% | intergenic (‑6/+90) | U712_19755 ← / ← U712_19760 | Transcription antiterminator LicT/Uncharacterized protein yxiP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 3,831,557 | 0 | G | A | 98.9% | 240.0 / ‑5.8 | 70 | intergenic (‑6/+90) | U712_19755/U712_19760 | Transcription antiterminator LicT/Uncharacterized protein yxiP |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (33/36); total (34/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGGCTTCATCGACATCATCGCCGGACTTTTTCTGAAACGCGAGCCCCCTGCCCATGACGACCAATTCTTTCCCCTGTTCATTGACCACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATGTCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCTTTTTGTACTAGGTCAATATCATCAGGGAAAGACGGAAGCTGGTCAGTACAATTCACTCCGTACCTGCTGCCCGGGCTGTTT > CP006881/3831425‑3831685 | gggCTTCATCGACATCATCGCCGGACTTTTTCTGAAACGCGAGCCCCCTGCCCATGACGACCAATTCTTTCCCCTGTTCATTGACCACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTc < 5:39371/139‑1 (MQ=255) gCTTCATCGACATCATCGCCGGACTTTTTCTGAAACGCGAGCCCCCTGCCCATGACGACCAATTCTTTCCCCTGTTCATTGACCACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTcc > 5:283980/1‑138 (MQ=255) cATCGACATCATCGCCGGACTTTTTCTGAAACGCGAGCCCCCTGCCCATGACGACCAATTCTTTCCCCTGTTCATTGACCACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAAt < 8:284936/138‑1 (MQ=255) cATCGACATCATCGCCGGACTTTTTCTGAAACGCGAGCCCCCTGCCCATGACGACCAATTCTTTCCCCTGTTCATTGACCACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAAt < 2:34092/138‑1 (MQ=255) cATCGACATCATCGCCGGACTTTTTCTGAAACGCGAGCCCCCTGCCCATGACGACCAATTCTTTCCCCTGTTCATTGACCACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAAt < 3:13108/138‑1 (MQ=255) gACATCATCGCCGGACTTTTTCTGAAACGCGAGCCCCCTGCCCATGACGACCAATTCTTTCCCCTGTTCATTGACCACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAATctact < 5:109230/139‑1 (MQ=255) gACATCATCGCCGGACTTTTTCTGAAACGCGAGCCCCCTGCCAATGACGACCAATTCTTTCCCCTGTTCATTGACCACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAATctact < 1:276725/139‑1 (MQ=255) tcatcGCCGGACTTTTTCTGAAACGCGAGCCCCCTGCCCATGACGACCAATTCTTTCCCCTGTTCATTGACCACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAg > 6:34687/1‑138 (MQ=255) atcGCCGGACTTTTTCTGAAACGCGAGCCCCCTGCCCATGACGACCAATTCTTTCCCCTGTTCATTGACCACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGaa < 1:251678/138‑1 (MQ=255) atcGCCGGACTTTTTCTGAAACGCGAGCCCCCTGCCCATGACGACCAATTCTTTCCCCTGTTCATTGACCACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGaa < 7:113374/138‑1 (MQ=255) atcGCCGGACTTTTTCTGAAACGCGAGCCCCCTGCCCATGACGACCAATTCTTTCCCCTGTTCATTGACCACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGaa < 2:191504/138‑1 (MQ=255) atcGCCGGACTTTTTCTGAAACGCGAGCCCCCTGCCCATGACGACCAATTCTTTCCCCTGTTCATTGACCACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGaa < 5:272321/138‑1 (MQ=255) atcGCCGGACTTTTTCTGAAACGCGAGCCCCCTGCCCATGACGACCAATTCTTTCCCCTGTTCATTGACCACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGaa < 5:240663/138‑1 (MQ=255) atcGCCGGACTTTTTCTGAAACGCGAGCCCCCTGCCCATGACGACCAATTCTTTCCCCTGTTCATTGACCACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGaa < 4:120202/138‑1 (MQ=255) atcGCCGGACTTTTTCTGAAACGCGAGCCCCCTGCCCATGACGACCAATTCTTTCCCCTGTTCATTGACCACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGAAc < 6:283980/139‑1 (MQ=255) ccGGACTTTTTCTGAAACGCGAGCCCCCTGCCCATGACGACCAATTCTTTCCCCTGTTCATTGACCACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGAACAGAc < 4:97576/139‑1 (MQ=255) acGGACGGTTTCTGAAACGCGAGCCCCCTGCCCATGACGACCAATTCTTTCCCCTGGTCATTGACCACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGAACAGAc < 8:25298/138‑1 (MQ=255) tttCTGAAACGCGAGCCCCCTGCCCCTGACGACCAATTCTTTCCCCTGTTCATTGACCACGCTGATCACATTATTTTTGCTCACCTTCGGAAATTTCATGGCATATCCCTCCAAATATACTAAGAACAGACTATAACtt > 3:255786/1‑139 (MQ=255) cTGAAACGCGAGCCCCCTGCCCATGACGACCAATTCTTTCCCCTGTTCATTGACCACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGAACAGCCTATATCTTGa < 2:265445/138‑1 (MQ=255) gcgAGCCCCCTGCCCATGACGACCAATTCTTTCCCCTGTTCATTGACCACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACAt < 3:164901/139‑1 (MQ=255) cccccTGCCCATGACGACCAATTCTTTCCCCTGTTCATTGACCACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCttt < 8:235455/139‑1 (MQ=255) ccccTGCCCATGACGACCAATTCTTTCCCCTGTTCATTGACCACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCtttt < 5:268920/139‑1 (MQ=255) cccATGACGACCAATTCTTTCCCCTGTTCATTGACCACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCTTTTTGTAc < 3:227750/138‑1 (MQ=255) ccAATTCTTTCCCCTGTTCATTGACCACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGaa > 5:169247/1‑94 (MQ=255) ccAATTCTTTCCCCTGTTCATTGACCACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGaa < 6:169247/94‑1 (MQ=255) ttCCCCTGTTCATTGACCACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCAAAATCAACTAAGAACAGACTATATCTTGATTGTACATTATTTTTTTACTAGTGCAAAATCATCAgg > 8:239151/1‑138 (MQ=255) tCCCCTGTTCATTGACCACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCTTTTTGTACTAGGTCAATATCATCAggg > 3:132279/1‑138 (MQ=255) tCCCCTGTTCATTGACCACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCTTTTTGTACTAGGTCAATATCATCAggg > 3:16696/1‑138 (MQ=255) ccccTGTTCATTGACCACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCTTTTTGTACTAGGTCAATATCATCAGGGa < 5:34687/138‑1 (MQ=255) tGTTCATTGACCACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCTTTTTGTACTAGGTCAATATCATCAGGGAAAGAc > 6:256798/1‑139 (MQ=255) tGTTCATTGACCACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCTTTTTGTACTAGGTCAATATCATCAGGGAAAGAc > 2:213770/1‑139 (MQ=255) gTTCATTGACCACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAAt > 3:103639/1‑69 (MQ=255) gTTCATTGACCACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAAt < 4:103639/69‑1 (MQ=255) ttCATTGACCACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCTTTTTGTACTAGGTCAATATCATCAGGGAAAGACg > 5:95136/1‑138 (MQ=255) ttCATTGACCACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCTTTTTGTACTAGGTCAATATCATCAGGGAAAGACg > 6:195828/1‑138 (MQ=255) tCATTGACCACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCTTTTTGTACTAGGTCAATATCATCAGGGAAAGACgg > 8:74051/1‑138 (MQ=255) aTTGACCACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCTTTTTGTACTAGGTCAATATCATCAGGGAAAGACGGAAg < 2:41036/139‑1 (MQ=255) tGACCACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAATctact > 3:230214/1‑68 (MQ=255) tGACCACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAATctact < 4:230214/68‑1 (MQ=255) ccACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCTTTTTGTACTAGGTCAATATCATCAGGGAAAGACGGAAGCTggg < 3:113077/139‑2 (MQ=255) ccACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCTTTTTGTACTAGGTCAATATCATCAGGGAAAGACGGAAGCTGGt < 7:74051/139‑1 (MQ=255) ccACGCTGAGCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCTTTTTGTACTAGGTCAATATCATCAGGGAAAGACGGAAGCTGGt < 5:152375/139‑1 (MQ=255) cTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCGAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCTTTTTGTACTAGGTCAATATCATCAGGGAAAGACGGAAGCTGGTCAGTa < 5:99367/139‑1 (MQ=255) caTTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCTTTTTGTACTAGGTCAATATCATCAGGGAAAGACGGAAGCTGGTCAGTACAATTCa < 7:239151/139‑1 (MQ=255) caTTATTGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCTTTTTGTACTAGGTCAATATCATCAGGGAAAGACGGAAGCTGGTCAGTACAATTCa < 6:130491/139‑1 (MQ=255) tattGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCTTTTTGTACTAg > 4:249906/1‑92 (MQ=255) tattGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCTTTTTGTACTAg < 3:249906/92‑1 (MQ=255) attGTTGATCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCTTTTTGTACTAGGTCAATATCATCAGGGAAAGACGGAAGCTGGTCAGTACAATTCACTcc > 7:269098/1‑139 (MQ=255) tgttgATCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCTTTTTGTACTAGGTCAATATCATCAGGGAAAGACGGAAGCTGGTCAGTACAATTCACTCCg > 1:204990/1‑138 (MQ=255) tgttgATCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCTTTTTGTACTAGGTCAATATCATCAGGGAAAGACGGAAGCTGCTCAGTACAATTCACTCCg > 8:237729/1‑138 (MQ=255) ttgATCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCTTTTTGTACTAGGTCAATATCATCAGGGAAAGACGGAAGCTGGTCAGTACAATTCACTCCGTAc > 4:215518/1‑139 (MQ=255) aTCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGaa > 3:60303/1‑47 (MQ=255) aTCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGaa < 4:60303/47‑1 (MQ=255) aTCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCTTTTTGTACTAGGTCAATATCATCAGGGAAAGACGGAAGCTGGTCAGTACAATTCACTCCGt < 3:241441/134‑1 (MQ=255) aTCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCTTTTTGTACTAGGTCAATATCATCAGGGAAAGACGGAAGCTGGTCAGTACAATTCACTCCGt > 4:241441/1‑134 (MQ=255) aTCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCTTTTTGTACTAGGTCAATATCATCAGGGAAAGACGGAAGCTGGTCAGTACAATTCACTCCGTACctg > 3:17784/1‑139 (MQ=255) aTCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCTTTTTGTACTAGGTCAATATCATCAGGGAAAGACGGAAGCTGGTCAGTACAATTCACTCCGTACctg > 1:271230/1‑139 (MQ=255) aTCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCTTTTTGTACTAGGTCAATATCATCAGGGAAAGACGGAAGCTGGTCAGTACAATTCACTCCGTACctg > 5:270515/1‑139 (MQ=255) aTCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCTTTTTGTACTAGGTCAATATCATCAGGGAAAGACGGAAGCTGGTCAGTACAATTCACTCCGTACctg > 7:162224/1‑139 (MQ=255) aTCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCTTTTTGTACTAGGTCAATATCATCAGGGAAAGACGGAAGCTGGTCAGTACAATTCACTCCGTACctg > 2:163496/1‑139 (MQ=255) aTCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCTTTTTGTACTAGGTCAATATCATCAGGGAAAGACGGAAGCTGGTCAGTACAATTCACTCCGTACctg > 4:149507/1‑139 (MQ=255) aTCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCTTTTTGTACTAGGTCAATATCATCAGGGAAAGACGGAAGCTGGTCAGTACAATTCACTCCGTACctg > 2:228681/1‑139 (MQ=255) aTCACCTTCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCTTTTTGTACTAGGTCAATATCATCAGGGAAAGACGGAAGCTGGTCAGTACAATTCACTCCGTACctg > 3:277450/1‑139 (MQ=255) aTCACCATCGCAATTTTCATGGCATGACCCTCCAAATCAACTAAGAACAGACTATATATTCATTGAACATTCTTTTTTTACTAGGTCAATATAATCAGGGAAAGACGGAAGCTCGTCAGTACAATTCACTCCGAACctt > 8:114502/1‑138 (MQ=255) tCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCTTTTTGTACTAGGTCa > 7:31941/1‑81 (MQ=255) tCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCTTTTTGTACTAGGTCa < 8:31941/81‑1 (MQ=255) tCGCAATTTTCATGGCATATCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCTTTTTGTACTAGGTCAATATCATCAGGGAAAGACGGAAGCTGGTCAGTACAATTCACTCCGTACCTGCTGCCCg > 3:189554/1‑139 (MQ=255) ttCATGGCATATCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCTTTTTGTACTAGGTCAATATCATCAGGGAAAGACGGAAGCTGGTCAGTACAATTCACTCCGTACCTGCTGCCCGGGCTGttt > 4:110550/1‑139 (MQ=255) aTGGCATATCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCTTTTTGTACTAGGTCAATATCATCAGGGAAAGACGGAAGCTGGTCAGTACAATTCACTCCGTACCTGCTGCCCGGGc < 7:234237/131‑1 (MQ=255) aTGGCATATCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCTTTTTGTACTAGGTCAATATCATCAGGGAAAGACGGAAGCTGGTCAGTACAATTCACTCCGTACCTGCTGCCCGGGc > 8:234237/1‑131 (MQ=255) | GGGCTTCATCGACATCATCGCCGGACTTTTTCTGAAACGCGAGCCCCCTGCCCATGACGACCAATTCTTTCCCCTGTTCATTGACCACGCTGATCACATTATTGTTGATCACCTTCGCAATTTTCATGGCATGTCCCTCCAAATCTACTAAGAACAGACTATATCTTGATTGTACATTCTTTTTGTACTAGGTCAATATCATCAGGGAAAGACGGAAGCTGGTCAGTACAATTCACTCCGTACCTGCTGCCCGGGCTGTTT > CP006881/3831425‑3831685 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |