BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	131	G→C	6.1%	intergenic (–/‑2847)	– / → eryR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	131	0	G	C	6.1%	18155.8 / 168.4	6733	intergenic (–/‑2847)	–/eryR	–/–
Reads supporting (aligned to +/- strand): ref base G (3132/2619); new base C (12/459); total (3152/3581)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 6752 total aligned reads displayed.

‑‑‑‑‑‑TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGC  >  pSIJ663_pHT315_araE_xy/1‑268

caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAATTGa                                                                                                                                          <  5:168383‑M1/132‑1 (MQ=255)

caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGa                                                                                                                                          <  6:288533‑M1/132‑1 (MQ=255)

caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGa                                                                                                                                          <  3:15594‑M1/132‑1 (MQ=255)

      tCCATCCTCCAATGGTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATACAACGGAAATGAAAAAAGc                                                                                                                                   <  2:244584/139‑1 (MQ=255)

      tCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGc                                                                                                                                   >  7:5674/1‑139 (MQ=255)

      tCCAACCTCCAACGTTGGAGAGGGAGTTTAAGGTCGCAAATATTAATGGTTCGGGTGAACCTTATCAAATTTTCGTTGATTAAATAGAAACATCGCGGTAAAA‑TT‑CCCAGAAACTAAATACACCGCAAATCAAAAAAGc                                                                                                                                   <  6:53087/139‑1 (MQ=255)

        cagcccccAAAGTTGGAGAGTGATTTTTATGTCGCAAAAATTAATGTTTCTGGTGAACCTTATCAAATTTTCTTTGATTTAATACAAACATAGCTGAAAAA‑TT‑AGCATAAAATTAATAGAACGCAAATAAAAAACGCCa                                                                                                                                 <  8:139756/133‑1 (MQ=255)

        cATCCTCCAAAGTTGGTGAGTGAGTTTTATGTCGAAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGTAAATGAAAAAAGCCa                                                                                                                                 <  8:276951/139‑1 (MQ=255)

        cATCCTCCAAAGTTGGAGTGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                 <  3:211106/139‑1 (MQ=255)

        cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTTAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                 <  5:112064/139‑1 (MQ=255)

        cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGACCGGAAATGAAAAAAGCCa                                                                                                                                 <  4:236224/139‑1 (MQ=255)

        cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                 >  8:80170/1‑139 (MQ=255)

        cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                 >  6:269719/1‑139 (MQ=255)

        cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                 >  2:191241/1‑139 (MQ=255)

        cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATACAACGGAAATGAAAAAAGCCa                                                                                                                                 <  3:284028/139‑1 (MQ=255)

          tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAACGCCAct                                                                                                                               >  4:167789/1‑139 (MQ=255)

           cctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                 <  1:51966/136‑1 (MQ=255)

           cctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCActc                                                                                                                              >  7:225473/1‑139 (MQ=255)

                      ggAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAt                                                                                                                   >  1:182349/1‑139 (MQ=255)

                       gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                 <  5:143360/124‑1 (MQ=255)

                       gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                                  >  2:30591/1‑139 (MQ=255)

                            tgagtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTa                                                                                                             >  2:146707/1‑139 (MQ=255)

                            tgagtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTa                                                                                                             >  1:164866/1‑139 (MQ=255)

                            tgagtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTa                                                                                                             >  7:172381/1‑139 (MQ=255)

                            tgagtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTa                                                                                                             >  1:100481/1‑139 (MQ=255)

                               gtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGTAAATTAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                           <  3:248373/138‑1 (MQ=255)

                                        cGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                                 >  4:139878/1‑139 (MQ=255)

                                          cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAGTAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                                 >  1:221539/1‑137 (MQ=255)

                                          cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                                 >  5:191221/1‑137 (MQ=255)

                                          cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                                 >  4:104341/1‑137 (MQ=255)

                                             ttattatTTTTTCTGGTTAACATTATCAAATTTTCGTTTATTTATTAGAAACATTGC‑GTAAAA‑TTAACCTGTAACTAATTTTAACGTAAATGAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                            <  4:69612/138‑1 (MQ=255)

                                             attttATTGTTTGTGGTCAACATTATCAAATTTTCTTTGTTTTAATAGAAAAATCGCTGAAAAA‑TT‑AGCAGAAAATTAAAACAACGTAAATCAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                            <  2:115486/136‑1 (MQ=255)

                                             atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                            <  4:124025/139‑1 (MQ=255)

                                             atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                            <  7:210148/139‑1 (MQ=255)

                                             atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGAAAAA‑TT‑AGCAGAAACTAAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                            <  3:116578/139‑1 (MQ=255)

                                             atatTAATGTTTCTGGTGAACCTTATAAAATTTTCGTTGATTTAAAAGAAACATACCGGAAAAA‑TA‑AGCAGTAACTAAATAGACCGGAAATCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                            <  7:278583/139‑1 (MQ=255)

                                             atatTAATGTTTCTGGTGAACATTATCAAATTTTCGTTGTTTTAAAAGAAACATAGGGGAAAAA‑TA‑AGCAGTAACTTAATAGACCGGAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                            <  6:206611/139‑1 (MQ=255)

                                              tatTAATGTTTCTGGTGAACCTTATAAAATTTTCGTTTATTTAATACAAAAATAGCGTTAAAA‑TT‑ACCTGAAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                            <  2:75959/138‑1 (MQ=255)

                                                   ttGTTTCTGGTCAACTTTATATATTTTTCGTTTATTTAATAGAAACATTGCGTTAAAA‑TT‑AGCATTAATTTAATAGACCGTAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                                       <  4:46615/137‑1 (MQ=255)

                                                   aTGTTTGTGTTTAACATTATAAAATTTTGGTTGTTTTAATCGAAACTTAGGGTTAAAA‑TT‑AGCTGTAAATTAAATTACGGGTAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                                       <  6:9788/138‑1 (MQ=255)

                                                   aTGTTTCTTGTTAACCTTATCAATTTTTCGTTGATTTAATACAAACATAGCGGAAAAA‑TT‑ACCAGTAACTAAATATAACGCAATTCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                                       <  1:91578/138‑1 (MQ=255)

                                                   aTGTTTCTGGTGAACCTTATCAAATTTTCGTTTATTTAATAGAAACATAGCGGAAAAA‑TT‑AGCCTTAAATTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                                       <  7:122052/138‑1 (MQ=255)

                                                   aTGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                                       <  5:27829/138‑1 (MQ=255)

                                                        tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGTTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                                 >  3:84251/1‑139 (MQ=255)

                                                        tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                           <  7:261471/113‑1 (MQ=255)

                                                        tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                           <  7:193000/113‑1 (MQ=255)

                                                        tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                                 >  3:196917/1‑139 (MQ=255)

                                                        tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                                 >  7:141289/1‑139 (MQ=255)

                                                         cTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACTGAATTGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCt                                                                                >  5:33750/1‑139 (MQ=255)

                                                         cTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                           <  5:129761/112‑1 (MQ=255)

                                                         cTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCt                                                                                >  1:202846/1‑139 (MQ=255)

                                                            gttaaacTTATAAAATTTTGTTTGATTAAATAAAAACATCG‑GGTAAAATTT‑AGAAGAAACTAAATATAACGTAAATCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                             <  6:181460/133‑1 (MQ=255)

                                                            gtcaaCATTATAAAATTTTCTTTGATTTAATACAAACATAGCGTTAAAA‑TT‑AGAATTAAATTAATAGAACGTAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTg                                                                               <  1:70697/134‑1 (MQ=255)

                                                            gTGACCCTTATCAAATTTTCGTTTATTTAATAGAAACATAGCGTTAAAT‑TT‑AGCAGTAAATTAATAGAAGGGAAATGAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                             <  5:23882/139‑1 (MQ=255)

                                                            gTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                             <  3:226896/139‑1 (MQ=255)

                                                             tGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTAc                                                                            <  8:200470/139‑1 (MQ=255)

                                                              gAACCTTATCAAATTTTCGTTGATTAAATAGAAACATAGCGGTAAAA‑AT‑AGCACTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGCCTACAAACCTATAGCGAGAATCACTTAAACCTGTACa                                                                           >  6:151836/1‑139 (MQ=255)

                                                               aaCCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                          >  5:215323/1‑139 (MQ=255)

                                                               aaCCTTATCAAATTTTCGTTGATTTAAAAGAAACATAGCGGTAAAA‑TT‑ACCAGTAACTAAAAAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                          <  5:55632/139‑1 (MQ=255)

                                                               aaCCTTAACAAATTATCGTTGATTAAATAGAAACAAACCGCAAAAA‑TA‑ACCAGAAACTAAATACAACGCAAATCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                          <  8:260022/139‑1 (MQ=255)

                                                                cacTTATAAAATTTTGGTTTATTTAATATAAACATAGCGTTAAAA‑TT‑CGCATTAACTTAATAGAACGCAAATTAAAACAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                          <  4:192025/136‑1 (MQ=255)

                                                                aCCTTATCAAATTTTCGTTGATTTAATAGAAACATCGCTGTAAAA‑TT‑CGCTGTAACTTAAAAGACCGCAATTTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                          <  7:97525/138‑1 (MQ=255)

                                                                aCCTTATAAATTTTTATTTCATTTAAAAGAAACATGGCGTTAAAA‑TT‑AGCAGTAACTTAATATCAGGGATTTGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTTGCGAGAATGACTTAATCCTGTACAg                                                                          <  7:78585/138‑1 (MQ=255)

                                                                 ccTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAAAAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGc                                                                         >  6:250533/1‑138 (MQ=255)

                                                                    tATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATa                                                                     >  1:121852/1‑139 (MQ=255)

                                                                     aTCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATa                                                                     <  6:132814/138‑1 (MQ=255)

                                                                     aTCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTAAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATAc                                                                    <  8:115868/139‑1 (MQ=255)

                                                                      tCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATAc                                                                    <  7:24236/138‑1 (MQ=255)

                                                                             ttCGTTTATTTAATAGAAACATAGCGGTAAAA‑TT‑ATCAGTAACTTAATATAACTTAAATTAAAAAAGCCACTCTCATAAGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                             <  4:93996/138‑1 (MQ=255)

                                                                             ttCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                             <  1:31393/138‑1 (MQ=255)

                                                                             ttCGTTGATTAAATAGAAACATCGCGGTAAAA‑TT‑AGCAGTAACTAAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                             <  6:229550/138‑1 (MQ=255)

                                                                              tCGTTGATTTAAAAGAAAAATAGCGTTAAAA‑TA‑AGCAGTAACTTAATATCACGGAAATCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTc                                                            <  1:246361/138‑1 (MQ=255)

                                                                                       tAATAGAAAAATCGGGTTAAAT‑TT‑AGCAGTATCTTAATCGCACGCACATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGTCTTCGACTTATaa                                                   <  2:170038/138‑1 (MQ=255)

                                                                                               aCATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                             <  2:5173/104‑1 (MQ=255)

                                                                                               aCATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGcc                                           >  4:282769/1‑138 (MQ=255)

                                                                                                    gCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACtt                                      >  8:59143/1‑138 (MQ=255)

                                                                                                       gTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                            <  7:155750/81‑1 (MQ=255)

                                                                                                        tAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCTAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCaaa                                  >  8:21895/1‑138 (MQ=255)

                                                                                                         aaaa‑TT‑ATCATTAAATTAATAGAACGCAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAAt                                 <  8:156946/138‑1 (MQ=255)

                                                                                                         aaaa‑TT‑AGCAGTAACTTAATACACCGTAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAAt                                 <  6:72236/138‑1 (MQ=255)

                                                                                                         aaaa‑TT‑AGAAGTAAATTATTAGAACGGAAATCAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAAt                                 <  2:214241/138‑1 (MQ=255)

                                                                                                                 aGCAGTAACTTAATAGACCGGAAATGAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTa                           <  4:260258/138‑1 (MQ=255)

                                                                                                                 aGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTAt                          >  7:36350/1‑139 (MQ=255)

                                                                                                                        aCTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCtt                   >  1:270378/1‑139 (MQ=255)

                                                                                                                        aCTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGACATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCtt                   >  3:177/1‑139 (MQ=255)

                                                                                                                            aaTAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGttt               >  3:133566/1‑139 (MQ=255)

                                                                                                                                gAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                            <  8:178887/58‑1 (MQ=255)

                                                                                                                                  aCGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGCCTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTcgcg          >  8:37962/1‑138 (MQ=255)

                                                                                                                                   cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTa                                                                                     >  3:267329/1‑62 (MQ=255)

                                                                                                                                   cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTg                                                                               <  6:117355/68‑1 (MQ=255)

                                                                                                                                   cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa        <  5:73515/139‑1 (MQ=255)

                                                                                                                                   cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa        >  1:144995/1‑139 (MQ=255)

                                                                                                                                   cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAAACTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa        >  5:260729/1‑139 (MQ=255)

                                                                                                                                   cGGAAATGAAAAAAGACACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa        <  5:97843/139‑1 (MQ=255)

                                                                                                                                    ggAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAAc       <  8:236427/139‑1 (MQ=255)

                                                                                                                                     taaaTCAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACa      <  2:98320/138‑1 (MQ=255)

                                                                                                                                     caaaTGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACa      <  3:62518/138‑1 (MQ=255)

                                                                                                                                      aaaTGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTc                                                            >  3:41079/1‑84 (MQ=255)

                                                                                                                                       aaTGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAg    <  4:212212/139‑1 (MQ=255)

                                                                                                                                       aaTGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAg    <  2:267556/139‑1 (MQ=255)

‑‑‑‑‑‑TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TT‑AGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGC  >  pSIJ663_pHT315_araE_xy/1‑268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`