BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	137	A→C	5.0%	intergenic (–/‑2841)	– / → eryR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	137	0	A	C	5.0%	16687.3 / 163.6	6554	intergenic (–/‑2841)	–/eryR	–/–
Reads supporting (aligned to +/- strand): ref base A (3110/2979); new base C (26/428); total (3145/3409)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 6809 total aligned reads displayed.

‑TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGCTTATTAG  >  pSIJ663_pHT315_araE_xy/1‑275

agacagcctccAAAGTTGGATTGTGAGTTTTTTGTCGCAAATTTTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTTATTTAATAGAAACATTGCGGTAAAATTC‑GCATAAAATTAATAGACCGCAAATTAAAAAAg                                                                                                                                           <  6:284756/133‑1 (MQ=255)

 tCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTTAATAGAACGGAAATGATAAAAGc                                                                                                                                          >  5:140221/1‑139 (MQ=255)

 tCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGc                                                                                                                                          >  7:5674/1‑139 (MQ=255)

 tCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGc                                                                                                                                          >  6:94484/1‑139 (MQ=255)

 tCCATCCTCAAAAGTGGGTGAGTGAGTTTTTTGTCGCAAATATTAATGTTTCTGGTGAACATTAAAAAATTTTCGTTTATTTAATAGAAACATCGCGTTAAAATTC‑GCCGAAACTAAATAGAACGCAAATCAAAAACGc                                                                                                                                          <  4:87050/139‑1 (MQ=255)

   cATCCTCCAAAGTTGGTGAGTGAGTTTTATGTCGAAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTTAATAGAACGTAAATGAAAAAAGCCa                                                                                                                                        <  8:276951/139‑1 (MQ=255)

   cATCCTCCAAAGTTGGCGCGTGAGTTTTATGTCGCAAATATTAATGTTGCTGGTGAACCTTATCAAATTTTCGTTGATTTAATACAAACATAGCGGTAAAATTA‑GCAGTAACTTAATCGACCGTAAATCAAAAAAGCCa                                                                                                                                        <  4:139247/139‑1 (MQ=255)

   cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGTTAAAATTA‑GCAGTAACTTAATAGAACGTAAATGAAAAAAGCCa                                                                                                                                        <  3:281284/139‑1 (MQ=255)

   cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                        <  3:39796/139‑1 (MQ=255)

   cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTATCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                        >  5:5598/1‑139 (MQ=255)

     tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                        >  5:107811/1‑137 (MQ=255)

     tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                                      >  3:107932/1‑139 (MQ=255)

          cAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTTAATAGATCGGAAATGAAAAAAGCCACTCTCa                                                                                                                                  >  7:138404/1‑138 (MQ=255)

          cAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCa                                                                                                                                  >  7:78854/1‑138 (MQ=255)

          cAAAGTTGGAGAGGGCGTTTTATGGCCCAAAAATTAATGTTTCGGGTCAACCTTATCAAATTTTCGTTTATTTAATAGAAACATCGCGGTAAAATTA‑GCAGTAACTAAATACAACGCAAATCAAAAAAGCCACTCTCat                                                                                                                                 <  6:267648/139‑1 (MQ=255)

          aatcgTTGGAGAGTTTGTTTTATGTCCAAAATATTTATGTTTCTGGTTAACCTTATCAAATTTTCGTTGATTTAATACAAACATAGCTGTAAATTTA‑GCAGTAAATTAATACAACGTAAATGAAAAAAGCCACTCTCat                                                                                                                                 <  4:188146/135‑1 (MQ=255)

           aaaGTTGGTGAGTTTGTTTTATGTCTAAAATATTAATTTTTCTGGTGAACCTTATAAATTTTTCGTTTATTTAATATAAAAATAGCGTTAAAATTA‑GCATTAACTTAATATAACGGAAATTAAAAAAGCCACTCTCata                                                                                                                                <  2:207518/139‑1 (MQ=255)

                 ggAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAt                                                                                                                          >  5:33615/1‑139 (MQ=255)

                  gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGcctgt                                                                                                                         >  6:134591/1‑135 (MQ=255)

                  gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                                         >  7:224910/1‑139 (MQ=255)

                  gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                                         >  2:46141/1‑139 (MQ=255)

                     tttgagtTTTATGTGGAAAATATTAATGTTTTTGGTGAACATTATCAAATTTTCGTTTATTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTTAATAGAACGTAAATGAAAAAAGCCACTCTCATATGCTATTGGc                                                                                                                      <  4:163924/137‑1 (MQ=255)

                       tgagtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTa                                                                                                                    >  1:100481/1‑139 (MQ=255)

                       tgagtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTAAATAGAACGGAAATGATAAAAGCCACTCTCATATGCTATTGGCTa                                                                                                                    >  3:265739/1‑139 (MQ=255)

                         agtTTTTTGCCGAAAATATTAAGGTTTCTGGTCACCCTTATCAAATTTTGGTTTTTTAATTAGAAACAAAGCGGTAAATTAT‑GAAGAAACTAAAAAGAACGCAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                                  <  6:69700/139‑1 (MQ=255)

                         agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAc                                                                                                                   >  3:80971/1‑138 (MQ=255)

                                  tCGAAATTATTATTTTTTCTGGTTAACCTAATCAATTTTTGTTTTATTTAATTTAAACATAGCGTTAAATTTC‑GCATTAAGTAATTATAACTCAAATCAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTa                                                                                                          <  6:11738/138‑1 (MQ=255)

                                   gacatatatTAATGTTTCTGGTGAACCTTATCAAATTTTCTTTGATTAAAAACAAACATAGCGGTAAAATTA‑CCAGTAACTAAATACAACGCAAATCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                                        <  4:278270/134‑1 (MQ=255)

                                   cGCAAATATAAATGTTGCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGTAAAAATTA‑GCAGAAACTAAATAGAACGCAATTGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                                        <  2:68927/139‑1 (MQ=255)

                                    gCAAATATTAATGTTTATGGTGAACATTATCAAATTTTCGTTTATTTAATACAAACATAGCGGTAAAATTA‑GCAGAAACTTAATAGAACGTAATTTAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                                        <  4:122922/138‑1 (MQ=255)

                                     cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                                        >  7:115823/1‑137 (MQ=255)

                                        atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                                   <  8:23781/139‑1 (MQ=255)

                                        atatTAATGTTTCTGGTCAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATAC‑GCAGTAACTAAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                                   <  5:21094/139‑1 (MQ=255)

                                         tattatTGTTTCTGGTGATCCTTATCAATTTTTCGTTGATTTAATAGAAACATTGCGGAAAATTTA‑CCAGTAATTTAATAGAAGGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                                   <  6:228826/138‑1 (MQ=255)

                                         tatTAATGTTTCTGGTGAACCTTAACAAATTTTCGTTTAATAAATACAAACATAGCTGAAAAATAA‑GCAGAAAATAAAAACACCGGAAATTAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAAt                                                                                                  <  1:289413/139‑1 (MQ=255)

                                             aaTGTTTCTGGTTAACCATATAAAATTTTCGTTGATTTAAAACAAACATAGCGGAAAAAATA‑GCATTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                                              <  8:117608/139‑1 (MQ=255)

                                              aTGTTTCTGGTGAACCTTATCAAATTTTCGTTGTTTTAATAGAAACATAGCGGTAAAATTA‑GGAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACtt                                                                                             <  7:98459/139‑1 (MQ=255)

                                              aTGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                                              <  4:254688/138‑1 (MQ=255)

                                              aTGTTTCTGGTGAACCTTATAAAATTTTCGTTCATTTATTAGAAACTTAGCGTTAAAATTA‑TCAGTATCTTATTTGAACGTAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                                              <  8:219088/138‑1 (MQ=255)

                                              aTGTTTCTGGTGAACATTATCAAATTTTCGTTGATTTAATATAAACATACCGGTAAAATTA‑GCATTAACTTAATAGAACGGAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                                              <  1:200681/138‑1 (MQ=255)

                                                gTTTCTGGTGAACCTTATAAAATTTTCGTTTATTTAAAATAAAAATAGCGGTAAAATTA‑GCATTAACTTAATAGAACGGAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTa                                                                                            <  2:138301/138‑1 (MQ=255)

                                                   tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTTAATAGAACGGAATTGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAGTGACTTAATca                                                                                        >  3:148737/1‑138 (MQ=255)

                                                   tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                                        >  3:129449/1‑139 (MQ=255)

                                                    cTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCt                                                                                       >  7:171005/1‑139 (MQ=255)

                                                     tGGTGAACCTTATAAAATTTTCTTTTATTTAATAGAAACATAGCGTTAAAATTA‑GCTGTAACTTAATAGACCGTAAATCAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTg                                                                                      <  4:207616/139‑1 (MQ=255)

                                                       gttaaCCTTATAAAATTTTCTTTGATTTAATAGAAACATAGCGGAAAAATTA‑GCAGAAACTTAATAGAACGCAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTg                                                                                      <  5:162093/134‑1 (MQ=255)

                                                       gtcaaCCTTATAAAATTTTCGTTGATTTAATACAAACATAGCTGTAAAATTA‑GCAGTAACTTAATATAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTg                                                                                      <  1:164165/134‑1 (MQ=255)

                                                       gTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                                    <  7:241571/139‑1 (MQ=255)

                                                       gTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCAGAAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                                    <  6:206351/139‑1 (MQ=255)

                                                          aaCCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTTAATAGAACGTAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                                 <  5:75071/139‑1 (MQ=255)

                                                          aaCCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTTAATAGAACGGAAATGATAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                                 >  7:19191/1‑139 (MQ=255)

                                                          aaCCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                                 >  8:8056/1‑139 (MQ=255)

                                                          aaCCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGAGAGAATGAGTTAATCATgaa                                                                                    >  1:217791/1‑134 (MQ=255)

                                                          aaCCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTTAATAGAACGGAAATCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                                 <  1:67903/139‑1 (MQ=255)

                                                           aCCTTATAAAATTTTCGTTTATTTAATATAAACATTGCGTTAAAATTA‑GCAGTAAATTAATAGAACTCAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                                 <  4:103229/138‑1 (MQ=255)

                                                            ccTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGcc                                                                               <  5:234636/139‑1 (MQ=255)

                                                              ttATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGctgt                                                                             >  7:261895/1‑136 (MQ=255)

                                                               tagcAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCTGTAACTTAATAGAACTGAAATGAAAAAAGCAACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCGATa                                                                            >  6:249545/4‑139 (MQ=255)

                                                               tATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATa                                                                            >  1:121852/1‑139 (MQ=255)

                                                               tATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATa                                                                            >  3:246462/1‑139 (MQ=255)

                                                                 tCAAATTTTCTTTGATTTAATATAAACATAGCGGTAAAATTC‑GCAGTAACTTAATAGAACGGAAATGAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACa                                                                          <  8:156492/139‑1 (MQ=255)

                                                                 tCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATa                                                                            >  8:58664/1‑137 (MQ=255)

                                                                 tCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATa                                                                            >  3:172135/1‑137 (MQ=255)

                                                                      ttttCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTg                                                                                      <  6:50435/122‑1 (MQ=255)

                                                                      ttttCGTTGATTTAATAGAAACATAGAGGTAAAATTA‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTCCCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACt                                                                     >  1:90346/1‑139 (MQ=255)

                                                                        ttCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTTAATCGACCGTAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                                    <  6:1561/138‑1 (MQ=255)

                                                                        ttCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                                    <  8:178420/138‑1 (MQ=255)

                                                                               aTTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTta                                                             >  1:267556/1‑138 (MQ=255)

                                                                                         aaaaTAGC‑GTAAAATTATCCATTAAATAAAAACAACGTAAATAAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGcc                                                  <  2:284213/136‑1 (MQ=255)

                                                                                         aaCATAGCGGTAAAATTA‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGgcgc                                                   >  2:91283/1‑138 (MQ=255)

                                                                                          aCATAGCGGTAAAATTA‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGcc                                                  >  2:235735/1‑138 (MQ=255)

                                                                                              aGCGGTAAAATTA‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACtt                                             >  4:64732/1‑139 (MQ=255)

                                                                                               gCGGTAAAATTA‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                                 >  1:180764/1‑102 (MQ=255)

                                                                                                  gTAAAATTA‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCaaa                                         >  6:181918/1‑139 (MQ=255)

                                                                                                   tAAAATTA‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATc                                                                                         <  7:280089/90‑1 (MQ=255)

                                                                                                      aaTTA‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAg                                     >  8:19995/1‑139 (MQ=255)

                                                                                                          a‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTAt                                 >  7:36350/1‑139 (MQ=255)

                                                                                                          a‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTAt                                 >  5:277930/1‑139 (MQ=255)

                                                                                                             cAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTAttt                               >  8:140095/1‑139 (MQ=255)

                                                                                                                tAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGcc                            >  2:185761/1‑139 (MQ=255)

                                                                                                                  aCTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                                   <  3:5989/66‑1 (MQ=255)

                                                                                                                       aTAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGtttt                     >  4:233479/1‑139 (MQ=255)

                                                                                                                          gAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGATAATGACTTAATCATGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTAAAATAAGTTAATTGCCTTGtttta                    >  5:187106/1‑136 (MQ=255)

                                                                                                                          gAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTcgc                  >  5:125850/1‑139 (MQ=255)

                                                                                                                             cagAAATGAAAAAAGCCACTCTCATATGCTATTGTCTACCAACCTTTATCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTCTTTTCGCGaa               >  3:224161/3‑139 (MQ=255)

                                                                                                                             cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                                   <  2:110627/55‑1 (MQ=255)

                                                                                                                             cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGcc                                                                               >  3:208105/1‑75 (MQ=255)

                                                                                                                             cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGac               >  5:258786/1‑138 (MQ=255)

                                                                                                                             cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa               >  1:241441/1‑139 (MQ=255)

                                                                                                                             cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa               >  8:259997/1‑139 (MQ=255)

                                                                                                                             cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa               >  8:37836/1‑139 (MQ=255)

                                                                                                                             cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa               >  7:209104/1‑139 (MQ=255)

                                                                                                                             cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa               >  1:50235/1‑139 (MQ=255)

                                                                                                                             cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa               >  2:164322/1‑139 (MQ=255)

                                                                                                                             cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa               <  2:259433/139‑1 (MQ=255)

                                                                                                                             cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa               >  7:153899/1‑139 (MQ=255)

                                                                                                                             cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa               >  4:40744/1‑139 (MQ=255)

                                                                                                                             cGGAAATGAAAAAAGACACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa               <  5:97843/139‑1 (MQ=255)

                                                                                                                                aaaTGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAAt                                        <  1:143032/111‑1 (MQ=255)

                                                                                                                                      aaaacGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGCtta      <  3:192027/139‑1 (MQ=255)

‑TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTA‑GCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGCTTATTAG  >  pSIJ663_pHT315_araE_xy/1‑275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`