BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	3,783	G→T	5.0%	intergenic (+68/‑88)	eryR → / → ampR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	3,783	0	G	T	5.0%	14915.1 / 100.6	4521	intergenic (+68/‑88)	eryR/ampR	–/–
Reads supporting (aligned to +/- strand): ref base G (1367/2779); new base T (294/65); total (1673/2848)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 4643 total aligned reads displayed.

AAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCA  >  pSIJ663_pHT315_araE_xy/3646‑3918

aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                                                         <  3:15734/138‑1 (MQ=255)

aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                                                         <  1:190365/138‑1 (MQ=255)

aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                                                         <  7:79035/138‑1 (MQ=255)

 aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                                                                       <  8:152828/139‑1 (MQ=255)

  gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACTGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                                                                      <  2:54207/139‑1 (MQ=255)

  gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                                                                      <  5:291850/139‑1 (MQ=255)

  gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                                                                      <  8:167894/139‑1 (MQ=255)

     ccATTACTTATGAGGAAGTCTTGTCTATATTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                                                                    <  2:139443/138‑1 (MQ=255)

       aTTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTTAATTTTTAAAGTTTAAAGTTACTAAAAGGAATTGAGATAAATTATtatatataat                                                                                                                                  >  8:36476/1‑136 (MQ=255)

            gacagAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                                                                            <  7:127792/135‑1 (MQ=255)

               tGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGc                                                                                                                         <  5:254676/139‑1 (MQ=255)

                gAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTTAATTTTGAAATTTAAAAGTTAATAAAGTGAATTGATATAAATTATTATATATAATAATGACACCt                                                                                                                        >  6:134047/1‑139 (MQ=255)

                gAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAATTTTTTAATTTTCACATTAATAAATGGAATTGAGATTAATTTTTTTATTTAAAAATGACAGcg                                                                                                                        >  4:240719/1‑138 (MQ=255)

                gAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAATTTTGAAAGTTTCACGTTAATAAATGGAATTGAGAAAAATTAATTTATATAATAATGAcctgt                                                                                                                        >  8:135004/1‑135 (MQ=255)

                    aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                                                    <  8:251056/139‑1 (MQ=255)

                    aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                                                    <  3:176302/139‑1 (MQ=255)

                     aGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaa                                                                                                                   <  4:106158/139‑1 (MQ=255)

                      gTATTGTTTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                                                                  <  7:232406/139‑1 (MQ=255)

                      gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                                                                  <  3:285758/139‑1 (MQ=255)

                      gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGATTCCaag                                                                                                                  <  7:288660/139‑1 (MQ=255)

                       tATTGTCTATTTTTAATTGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGTAAGTTAGACGTTACTAAAGGTAATGGAGATAAATTATTAGATATACTTCTGACAGCTTCCaag                                                                                                                  <  2:78031/138‑1 (MQ=255)

                         ttGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaagaag                                                                                                               <  6:209141/139‑1 (MQ=255)

                         ttGTATATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaagaag                                                                                                               <  8:213250/139‑1 (MQ=255)

                            tCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTAAATTTTGTAATTTTAACCTTAATAAATTGAATGTATTTAAATTATTATATAAAATAATTACAGCTTGCaataacca                                                                                                             >  2:233111/1‑135 (MQ=255)

                                 ttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAATTTTTTAATTTAAAACTGAATAAATGGGATGGAGATAAATTATTAGATATAAAACTTACATCATCCAAGAAACaaaat                                                                                                         >  6:258485/1‑136 (MQ=255)

                                  tttAATAGTTATCTTTTATTTATCGGGAGGAAATTATTCTATGAGGCGGTTTTTTAAATTTGGAATTTTACAAGTTAGTAAAGGTATTGTAGATAATTTTTTAGATATACTACTGACAGCTTCCAAGAAGCTAAtgag                                                                                                       <  4:19397/138‑1 (MQ=255)

                                  tttAATAGTTATCTATTATTTAACTGTAGTAAATAATTCTATGAGTCGATTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                                                       <  1:65004/138‑1 (MQ=255)

                                  tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                                                       <  7:179368/138‑1 (MQ=255)

                                  tttAATAGTTATCTATTATTTAACGGGACGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                                                       <  1:17382/138‑1 (MQ=255)

                                          ttATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCTCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAATAGGTCCCTAg                                                                                               <  3:64742/138‑1 (MQ=255)

                                                    tttAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTTAATTTTGAAAATTTAACCTTAATAAACGGAAAGGAGCTAAATTATTTTTTATAATAAAGACAACTTCCAAAAAGCTAAACAGGTACCCAGCGCCTaacggg                                                                                    >  4:218293/1‑134 (MQ=255)

                                                    tttAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACggg                                                                                     <  3:222392/138‑1 (MQ=255)

                                                    tttAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACggg                                                                                     <  7:46413/138‑1 (MQ=255)

                                                    tttAACGGGAGGAAATAATTCTAGGAGTCGCTTTTTTAAATTTGGAAGCTTACACGTTACTAAAGGGAATTGAGAAAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACggg                                                                                     <  1:120458/138‑1 (MQ=255)

                                                       aaCGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAATTTTGAAAGTTACACGTTTCTAAAGGGAATTGAGATAAAATATTAGATATAATAATGAAAGCATCCAAGAAGCTAAAGAGGTCCCAAGCCCCGACGGGGAAt                                                                                 >  7:27287/1‑139 (MQ=255)

                                                           ggaggaAATAATTCTATGAGTCGCGGTTTTTAATTTTTTTATTTTAAAGTTTATAAAGGGGATTGAGTTATATTTTTATATTTTAGAATGTCCGCTTCCAAGATCCTAAAGAGGTCCCGAGCGCCGACGGGGAATTTgg                                                                             >  2:122531/1‑138 (MQ=255)

                                                            gaggaAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgg                                                                            <  5:1708‑M1/139‑3 (MQ=255)

                                                                   taatTATATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacatt                                                                     <  4:69369‑M1/139‑10 (MQ=255)

                                                                        cTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaa                                                                 <  3:45034‑M1/138‑14 (MQ=255)

                                                                        cTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaa                                                                 <  3:85365‑M1/138‑14 (MQ=255)

                                                                           tGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                             <  7:214873‑M1/139‑18 (MQ=255)

                                                                           tGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                             <  5:129261‑M1/139‑18 (MQ=255)

                                                                           tGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                             <  1:156560‑M1/139‑18 (MQ=255)

                                                                           tGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                             <  3:139897‑M1/139‑18 (MQ=255)

                                                                           tGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaa                                                                <  7:63960‑M1/136‑15 (MQ=255)

                                                                           tGAGTCGCGTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                             <  2:205511‑M1/139‑18 (MQ=255)

                                                                            gAGTCTATTTTTTAATTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAAATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                             <  3:263883‑M1/138‑18 (MQ=255)

                                                                            gAGTCGCTTTTTTAAATTTGGAAAGTTACAAGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgtgtacattgaaaaagg                                                             <  5:226484‑M1/138‑18 (MQ=255)

                                                                            gAGTCGATTTTTTAAATTTGTAAAGTTACACGTTACTAAAGGTAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                             <  1:42415‑M1/138‑18 (MQ=255)

                                                                             aGTCGCTTTTTTAAATTTGGAAAGTTCCACGTTACTAAAGGGAATGGAGAAAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAACGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                                                           <  8:114872‑M1/139‑20 (MQ=255)

                                                                             aGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                                                           <  6:7134‑M1/139‑20 (MQ=255)

                                                                             aGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                                                           <  5:48365‑M1/139‑20 (MQ=255)

                                                                                     ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                     <  6:47266‑M1/137‑26 (MQ=255)

                                                                                     ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                     <  5:149500‑M1/137‑26 (MQ=255)

                                                                                     ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                     <  6:232191‑M1/137‑26 (MQ=255)

                                                                                         aaaTTTGTAAATTTACACGTTACTAAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta                                                <  5:182343‑M1/138‑31 (MQ=255)

                                                                                         aaaTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTGAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta                                                <  1:136211‑M1/138‑31 (MQ=255)

                                                                                          aaTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtatt                                              <  4:140454‑M1/139‑33 (MQ=255)

                                                                                              tGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGATAAAGAGGTCCCTAGAGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcact                                          >  5:110622‑M1/1‑103 (MQ=255)

                                                                                                        cacGTTACTAAAGGGAATGGAGATAAATTATTAGATATCCTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgt                                <  7:113093‑M1/139‑47 (MQ=255)

                                                                                                             tACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccc                           >  7:250249‑M1/1‑88 (MQ=255)

                                                                                                               cTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAATAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccct                          >  7:148479‑M1/1‑86 (MQ=255)

                                                                                                               cTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccct                          >  6:96319‑M1/1‑86 (MQ=255)

                                                                                                               cTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccct                          >  1:217093‑M1/1‑86 (MQ=255)

                                                                                                               cTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccct                          >  1:255970‑M1/1‑86 (MQ=255)

                                                                                                               cTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                             >  4:168171‑M1/1‑86 (MQ=255)

                                                                                                               cTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                             <  5:20220‑M1/103‑18 (MQ=255)

                                                                                                                     ggAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattacc                   >  8:60252‑M1/1‑80 (MQ=255)

                                                                                                                     ggAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta                                                <  2:288148‑M1/110‑31 (MQ=255)

                                                                                                                       attggAGATAAATTATTAGATATCCTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGTGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcacta                 >  1:82415‑M1/3‑78 (MQ=255)

                                                                                                                       aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggatgagtatgagtattcaacatttccgagtcgcccttattcccta                 >  5:71216‑M1/1‑78 (MQ=255)

                                                                                                                       aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                 >  6:155842‑M1/1‑78 (MQ=255)

                                                                                                                       aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                 >  1:23859‑M1/1‑78 (MQ=255)

                                                                                                                       aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                 >  3:200583‑M1/1‑78 (MQ=255)

                                                                                                                       aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaaca                                         <  8:274147‑M1/115‑38 (MQ=255)

                                                                                                                       aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatcagtattcaacatttccgtgtcgcccttattccctt                 >  7:199874‑M1/1‑78 (MQ=255)

                                                                                                                       aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                                                       >  7:163286‑M1/1‑78 (MQ=255)

                                                                                                                       aaTGGAGATAAATTATTAGATAAACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                             <  3:119337‑M1/95‑18 (MQ=255)

                                                                                                                       aaTGGAGATAAATTATTAGAAAAACTACTGACACCTTCCCAGAAGCTAAAGAGGTACCTAGCGCCTACGGGGAAT‑‑‑TA‑‑GGGGTACATTGAATTAGGATGAGTATGAGTATACACCATTTCCGTGTCGCCCTTATTCCCtt            >  8:187992/1‑139 (MQ=255)

                                                                                                                         tcgagataAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGACCCTAGCGCCTACGCGGAACTACgcgtacatagaaaaaggaagagtatgagtattcaacattaccgagacgcccttattcccattt               >  1:97689‑M1/3‑76 (MQ=255)

                                                                                                                         tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctttt               >  4:50442‑M1/1‑76 (MQ=255)

                                                                                                                         tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctttt               >  5:90854‑M1/1‑76 (MQ=255)

                                                                                                                         tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctttt               >  1:100196‑M1/1‑76 (MQ=255)

                                                                                                                         tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctttt               >  2:159629‑M1/1‑76 (MQ=255)

                                                                                                                         tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctttt               >  6:283902‑M1/1‑76 (MQ=255)

                                                                                                                         tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctttt               >  2:180427‑M1/1‑76 (MQ=255)

                                                                                                                         tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctttt               >  4:90666‑M1/1‑76 (MQ=255)

                                                                                                                         tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                             >  1:279710‑M1/1‑76 (MQ=255)

                                                                                                                             gaTAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgc           <  3:243778‑M1/139‑68 (MQ=255)

                                                                                                                              aTAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                             <  4:290630‑M1/88‑18 (MQ=255)

                                                                                                                                aaaTTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcgg         >  2:98237‑M1/1‑69 (MQ=255)

                                                                                                                                aaaTTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcgg         >  7:9446‑M1/1‑69 (MQ=255)

                                                                                                                                 aaTTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggca       >  4:124386‑M1/1‑68 (MQ=255)

                                                                                                                                 aaTTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggca       >  6:115151‑M1/1‑68 (MQ=255)

                                                                                                                                 aaTTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                     <  4:26628‑M1/93‑26 (MQ=255)

                                                                                                                                  attattAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcat      >  2:255183‑M1/1‑67 (MQ=255)

                                                                                                                                   ttattaGATATACTACTGACATCTTCCAAGAAGCTAAAGAGGGCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcatt     <  5:26996‑M1/139‑74 (MQ=255)

                                                                                                                                      ttaGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttg  >  8:23272‑M1/1‑63 (MQ=255)

                                                                                                                                      ttaGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaa                                                                 >  8:81667‑M1/1‑63 (MQ=255)

                                                                                                                                        aGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                                                       >  6:180775‑M1/1‑61 (MQ=255)

AAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCA  >  pSIJ663_pHT315_araE_xy/3646‑3918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`