Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 1,538,461 T→C 7.8% S54P (TCG→CCG)  U712_07945 → Uncharacterized protein ylbN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068811,538,4610TC 7.8% 141.8 / 4.6 51S54P (TCG→CCG) U712_07945Uncharacterized protein ylbN
Reads supporting (aligned to +/- strand):  ref base T (16/31);  new base C (2/2);  total (18/33)
Fisher's exact test for biased strand distribution p-value = 6.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.71e-01

GCATCAAATGCCAAAACAAAGTTTTGAGTTTGATGAAACAGTTGAGTTAAATGAGCTGACAAAGCTGAATTCTGACATTCGCCGCATTTCTCCCGTTCGGGTGAAGGGCAGAGCGGATATCAAATCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGCCATGCTCAAGAACACTCGTTGATGTTCCGTATCCATTTGAAATTTCAACAAAAGAACTGTTTATATTTCATCACACGGATGATATAGAAGATGATGACGT  >  CP006881/1538325‑1538597
                                                                                                                                        |                                                                                                                                        
gCATCAAATGCCAAAACAAAGTTTTGAGTTTGATGAAACAGTTGAGTTAAATGAGCTGACAAAGCTGAATTCTGACATTCGCAGCATTTCTCCCGTTCGGGTGAAGGGCAGAGCGGATATCAAATCCAAACAGGATTCg                                                                                                                                        >  1:209916/1‑139 (MQ=255)
         gCCAAAACAAAGTTTTGAGTTTGATGAAACAGTTGAGTTAAATGAGCTGACAAAGCTGAATTCTGACATTCGCCGCATTTCTCCCGTTCGGGTGAAGGGCAGAGCGGATATCAAATCCAAACAGGTTTCGTTTGAttt                                                                                                                                >  4:249448/1‑138 (MQ=255)
                        tGAGTTTGATGAAACAGTTGAGTTAAATGAGCTGACAAAGCTGAATTCTGACATTCGCCGCATTTCTCCCGTTCGGGTGAAGGGCAGAGCGGATATCAAATCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGa                                                                                                                 >  3:97391/1‑138 (MQ=255)
                         gtgTTTGATGAAACAGTTGAGTTAAATGAGCTGACAATGGTGAATTCTGACATTCGCCGAATTTCTCCCGTTCGGGGGAAGGGCAGAGCGGATATCAAATCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGaa                                                                                                                <  4:148007/136‑1 (MQ=255)
                         gAGTTTGATGAAACAGTTGAGTTAAATGAGCTGACAAAGCTGAATTCTGACATTCGCCGCATTTCTCCCGTTCGGGTGAAGGGCAGAGCGGATATCAAATCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGaa                                                                                                                <  8:248335/138‑1 (MQ=255)
                          aGTTTGATGAAACAGTTGAGTTAAATGAGCTGACAAAGCTGAATTCTGACATTCGCCGCATTTCTCCCGTTCGGGTGAAGGGCAGAGCGGATATCAAATCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGaaa                                                                                                               <  7:117346/138‑1 (MQ=255)
                                   aaaCAGTTGAGTTAAATGAGCTGACAAAGCTGAATTCTGACATTCGCCGCATTTCTCCCGTTCGGGTGAAGGGCAGAGCGGATATCAAATCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGcc                                                                                                     <  1:289017/139‑1 (MQ=255)
                                   aaaCAGTTGAGTTAAATGAGCTGACAAAGCTGAATTCTGACATTCGCCGCATTTCTCCCGTTCGGGTGAAGGGCAGAGCGGATATCAAATCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGcc                                                                                                     <  8:215623/139‑1 (MQ=255)
                                   aaaCAGTTGAGTTAAATGAGCTGACAAAGCTGAATTCTGACATTCGCCGCATTTCTCCCGTTCGGGTGAAGGGCAGAGCGGATATCAAATCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGcc                                                                                                     <  3:42789/139‑1 (MQ=255)
                                    aaCAGTTGAGTTAAATGAGCTGACAAAGCTGAATTCTGACATTCGCCGCATTTCTCCCGTTCGGGTGAAGGGCAGAGCGGATATCAAATCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGcc                                                                                                     <  6:183054/138‑1 (MQ=255)
                                         ttGAGTTAAATGAGCTGACAAAGCTGAATTCTGACATTCGCCGCATTTCTCCCGTTCGGGTGAAGGGCAGAGCGGATATCAAATCCAAAGAGGTTTCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGCCATGCt                                                                                                >  2:281736/1‑138 (MQ=255)
                                         ttGAGTTAAATGAGCTGACAAAGCTGAATTCTGACATTCGCCGCATTTCTCCCGTTCGGGTGAAGGGCAGAGCGGATATCAAATCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGCCATGCt                                                                                                >  6:129596/1‑138 (MQ=255)
                                              ttAAATGAGCTGACAAAGCTGAATTCTGACATTCGCCGCATTTCTCCCGTTCGGGTGAAGGGCAGAGCGGATATCAAATCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGCCATGCTCAAGaa                                                                                          <  2:162590/139‑1 (MQ=255)
                                               tAAATGAGCTGACAAAGCTGAATTCTGACATTCGCCGCATTTCTCCCGTTCGGGTGAAGGGCAGAGCGGATATCAAATCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGCCATGCTCAAGaa                                                                                          <  2:209916/138‑1 (MQ=255)
                                                     aGCTGACAAAGCTGAATTCTGACATTCGCCGCATTTCTCCCGTTCGGGTGAAGGGCAGAGCGGATATCAAATCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGCCATGCTCAAGAACACTCg                                                                                    <  4:99035/138‑1 (MQ=255)
                                                      gCTGACAAAGCTGAATTCTGACATTCGCCGCATTTCTCCCGTTCGGGTGAAGGGCAGAGCGGATATCAAATCCAAACAGGTTCCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGCCATGCTCAAGAACACt                                                                                      <  8:62753/135‑1 (MQ=255)
                                                      gCTGACAAAGCTGAATTCTGACATTCGCCGCATTTCTCCCGTTCGGGTGAAGGGCAGAGCGGATATCAAATCCAAACAGGTTCCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGCCATGCTCAAGAACACt                                                                                      >  7:62753/1‑135 (MQ=255)
                                                       cTGACAAAGCTGAATTCTGACATTCGCCGCATTTCTCCCGTTCGGGTGAAGGGCAGAGCGGATATCAAATCCAAACAGGTTTCGTTTGATTTTTCAATTTCAGGCGAAATGCTTTTGCCATGCTCAAGAACACTCGTTg                                                                                 >  1:251391/1‑139 (MQ=255)
                                                       cTGACAAAGCTGAATTCTGACATTCGCCGCATTTCTCCCGTTCGGGTGAAGGGCAGAGCGGATATCAAATCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGCCATGCTCAAGAACACTCGTTg                                                                                 <  8:208416/139‑1 (MQ=255)
                                                       cTGACAAAGCTGAATTCTGACATTCGCCGCATTTCTCCCGTTCGGGTGAAGGGCAGAGCGGATATCAAATCCAAACAGGTTTAGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGCCATGCTCAAGAACACTCGTTg                                                                                 <  8:177253/139‑1 (MQ=255)
                                                               gCTGAATTCTGACATTCGCCGCATTTCTCCCGTTCGGGTGAAGGGCAGAGCGGATATCAAATCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGCCa                                                                                                    >  8:74901/1‑112 (MQ=255)
                                                               gCTGAATTCTGACATTCGCCGCATTTCTCCCGTTCGGGTGAAGGGCAGAGCGGATATCAAATCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGCCa                                                                                                    <  7:74901/112‑1 (MQ=255)
                                                                          aCATTCGCCGCATTTCTCCCGTTCGGGTGAAGGGCAGAGCGGATATCAAATCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGCCATGCTCAAGAACACTCGTTGAGGGTCCGTATCCATTTg                                                               >  7:51689/1‑138 (MQ=255)
                                                                           cATTCGCCGCATTTCTCCCGTTCGGGTGAAGGGCAGAGCGGATATCAAATCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGCCATGCTCAAGAACACTCGTTGATGTTCCGTATCCATTTGaa                                                             <  4:136817/139‑1 (MQ=255)
                                                                                            ccGTTCGGGTGAAGGGCAGAGCGGATATCAAATCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGCCATGCTCAAGAACACTCGTTGATGTTCCGTATCCATTTGAAATTTCAACAAAAGaa                                              <  7:243979/137‑1 (MQ=255)
                                                                                            ccGTTCGGGTGAAGGGCAGAGCGGATATCAAATCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGCCATGCTCAAGAACACTCGTTGATGTTCCGTATCCATTTGAAATTTCAACAAAAGaa                                              <  1:178795/137‑1 (MQ=255)
                                                                                            ccGTTCGGGTGAAGGGCAGAGCGGATATCAAATCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGCCATGCTCAAGAACACTCGTTGATGTTCCGTATCCATTTGAAATTTCAACAAAAGaa                                              <  3:102424/137‑1 (MQ=255)
                                                                                            ccGTTCGGGTGAAGGGCAGAGCGGATATCAAATCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGCCATGCTCAAGAACACTCGTTGATGTTCCGTATCCATTTGAAATTTCAACAAAAGaa                                              <  3:249448/137‑1 (MQ=255)
                                                                                            ccGTTCGGGTGAAGGGCAGAGCGGATATCAAATCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGCCATGCTCAAGAACACTCGTTGATGTTCCGTATCCATTTGAAATTTCAACAAAAGaa                                              <  7:257532/137‑1 (MQ=255)
                                                                                            ccGTTCGGGTGAAGGGCAGAGCGGATATCAAATCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGCCATGCTCAAGAACACTCGTTGATGTTCCGTATCCATTTGAAATTTCAACAAAAGaa                                              <  7:227694/137‑1 (MQ=255)
                                                                                            ccGTTCGGGTGAAGGGCAGAGCGGATATCAAATCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGCCATGCTCAAGAACACTCGTTGATGTTCCGTATCCATTTGAAATTTCAACAAAAGa                                               <  4:97391/136‑1 (MQ=255)
                                                                                               ttCGGGTGAAGGGCAGAGCGGATATCAAATCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGATATGCTTTTGCCATGCTCAAGAACACTCGTTGATGTTCCGTATCCATTTGAAATTTCAACAAAAGAACTGtt                                         <  8:13894/139‑1 (MQ=255)
                                                                                               ttCGGGTGAAGGGCAGAGCGGATATCAAATCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGCCATGCTCAAGAACACTCGTTGATGTTCCGTATCCATTTGAAATTTCAACAAAAGAACTGtt                                         >  7:226975/1‑139 (MQ=255)
                                                                                                   ggTGAAGGGCAGAGCGGATATCAAATCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGCCATGCTCAAGAACACTCGTTGATGTTCCGTATCCATTTGAAATTTCAACAAAAGAACTGTTtata                                     <  8:123306/139‑1 (MQ=255)
                                                                                                    gTGAAGGGCAGAGCGGATATCAAATCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGCCATGCTCAAGAACACTCGTTGATGTTCCGTATCCATTTGAAATTTCAACAAAAGAACTGTTTatat                                    >  4:109649/1‑139 (MQ=255)
                                                                                                              gagCGGATATCAAATCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGCCATGCTCAAGAACACTCGTTGATGTTCCGTATCCATTTGAAATTTCAACAAAAGAACTGTTTATATTTCATCacac                          >  4:176623/1‑139 (MQ=255)
                                                                                                               agCGGATATCAAATCCAAACAGGTTCCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGCCATGCTCAAGAACACTCGTTGATGTTCCGTATCCATTTGAAATTTCAACAAAAGAACTGTTTATATTTCATAACACg                         >  6:166191/1‑139 (MQ=255)
                                                                                                                 cGGATATCAAATCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGCCATGCTCAAGAACACTCGTTGATGTTCCGTATCCATTTGAAATTTCAACAAAAGAACTGTTTATATTTCATCACACGga                       <  5:129596/139‑1 (MQ=255)
                                                                                                                 cGGATATCAAATCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGCCATGCTCAAGAACACTCGTTGATGTTCCGTATCCATTTGAAATTTCAACAAAAGAACTGTTTATATTTCATCACACGga                       <  6:71121/139‑1 (MQ=255)
                                                                                                                 cGGATATCAAATCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGCCATGCTCAAGAACACTCGTTGATGTTCCGTATCCATTTGAAATTTCAACAAAAGAACTGTTTATATTTCATCACACGga                       <  2:226376/139‑1 (MQ=255)
                                                                                                                 cGGATATCAAATCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGCCATGCTCAAGAACACTCGTTGATGTTCCGTATCCATTTGAAATTTCAACAAAAGAACTGTTTATATTTCATCACACGga                       <  8:226975/139‑1 (MQ=255)
                                                                                                                   gATATCAAATCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGCCATGCTCAAGAACACTCGTTGATGTTCCGTATCCATTTGAAATTTCAACAAAAGAACTGTTTATATTTCATCACACGgatg                     <  5:289834/139‑1 (MQ=255)
                                                                                                                    atatCAAATCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGCCATGCTCAAGAACACTCGTTGATGTTCCGTATCCATTTGAAATTTCAACAAAAGAACTGTTTATATTTCATCACACGgatga                    >  1:53859/1‑139 (MQ=255)
                                                                                                                       tCAAATCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGCCATGCTCAAGAACACTCGTTGATGTTCCGTATCCATTTGAAATTTCAACAAAAGAACTGTTTATATTTCATCACACGGATGata                  <  3:109649/138‑1 (MQ=255)
                                                                                                                         aaaTCCACACAGGTTTCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGCCATGCTCAAGAACACTCGTTGATGTTCCGTATCCATTTGATATTTAAACAAAAGAACTGTTTATATTTCATCACACGGATGata                  >  1:223646/1‑136 (MQ=255)
                                                                                                                         aaaTCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGCCATGCTCAAGAACACTCGTTGATGTTCCGTATCCATTTGAAATTTCAACAAAAGAACTGTTTATATTTCATCACACGGATGAtata                <  4:211822/138‑1 (MQ=255)
                                                                                                                         aaaTCCAAACAGGTTCCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGCCATGCTCAAGAACACTCGTTGATGTTCCGTATCCATTTGAAATTTCAACAAAAGAACTGTTTATATTTCATCACACGGATGAtata                <  5:166191/138‑1 (MQ=255)
                                                                                                                           aTCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGCCATGCTCAAGAACACTCGTTGATGTTCCGTATCCATTTGAAATTTCAACAAAAGAACTGTTTATATTTCATCACACGga                       <  6:42728/129‑1 (MQ=255)
                                                                                                                           aTCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGCCATGCTCAAGAACACTCGTTGATGTTCCGTATCCATTTGAAATTTCAACAAAAGAACTGTTTATATTTCATCACACGGATGATATag               >  8:275806/1‑137 (MQ=255)
                                                                                                                           aTCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGCCATGCTAAAGAACACTCGTTGATGTTCCGTATCCATTTGAAATTTCAACAAAAGAACTGTTTATATTTCATCACACGga                       >  5:42728/1‑129 (MQ=255)
                                                                                                                                     gTTTCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGCCATGCTCAAGAACACTCGTTGATGTTCCGTATCCATTTGAAATTTCAACAAAAGAACTGTTTATATTTCATCACACGGATGATATAGAAGATGATGACg   >  4:238439/1‑139 (MQ=255)
                                                                                                                                      tttCGTTTGATTTTACAAATTCAGGCGAAATGCTTTTGCCATGCTCAAGAACACTCGTTGATGTTCCGTATCCATTTGAAATTTCAACAAAAGAACTGTTTATATTTCATCACACGGATGATATAGAAGATGATGACGt  <  1:28759/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                        
GCATCAAATGCCAAAACAAAGTTTTGAGTTTGATGAAACAGTTGAGTTAAATGAGCTGACAAAGCTGAATTCTGACATTCGCCGCATTTCTCCCGTTCGGGTGAAGGGCAGAGCGGATATCAAATCCAAACAGGTTTCGTTTGATTTTACAATTTCAGGCGAAATGCTTTTGCCATGCTCAAGAACACTCGTTGATGTTCCGTATCCATTTGAAATTTCAACAAAAGAACTGTTTATATTTCATCACACGGATGATATAGAAGATGATGACGT  >  CP006881/1538325‑1538597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑