BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	4,648	T→A	10.5%	Y260N (TAC→AAC) ‡	ampR →

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	4,648	0	T	A	10.5%	10147.7 / 280.7	3785	Y260N (TAC→AAC) ‡	ampR
Reads supporting (aligned to +/- strand): ref base T (2477/851); new base A (7/422); total (2487/1298)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3848 total aligned reads displayed.

TTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTT  >  pSIJ663_pHT315_araE_xy/4511‑4786

 tGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTa                                                                                                                                           >  4:218470/1‑138 (MQ=255)

  gcagcaCCACTTCTGCGCTCGGCCCTTCGGGCGGGCGGTTTTATTCCTCATAAATCGGGAGCCGGTGAGGGGGGGGCTCGCGTTATCATTGCACCACTGGGGCCAGATGGTAACCCCTCCCGTATCGTAGTTATCAac                                                                                                                                          <  8:273537/138‑1 (MQ=255)

  gCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTaca                                                                                                                                         >  7:191513/1‑139 (MQ=255)

   cAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTacac                                                                                                                                        >  1:253241/1‑139 (MQ=255)

                 cgcTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAgg                                                                                                                          >  2:163260/1‑139 (MQ=255)

                 cgcTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAgg                                                                                                                          >  3:277569/1‑139 (MQ=255)

                  gcTCGGCCCTGCCGGCTGGGTGGTTTATTGCTGAAAAATCGGGCGCGGGGGAGGGGGGGGCGCGGGTTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTAATCACCACGACGGGGAGTCAGGc                                                                                                                         <  6:277833/139‑1 (MQ=255)

                   cTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAGTCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGc                                                                                                                         >  1:129097/1‑138 (MQ=255)

                   cTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGc                                                                                                                         >  4:198978/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTCTCGTAGTTATCTACACGACGGGGAGTCAGGCATCt                                                                                                                     >  1:100031/1‑139 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACCCGACGGGGAGTCAGGCAAc                                                                                                                      >  5:215002/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACCCGACGCGGAGTCAGGCAAc                                                                                                                      >  4:265020/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                      >  6:36089/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                      >  7:256303/1‑138 (MQ=255)

                            tCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGACAGGCAACTATGGAt                                                                                                               >  3:246681/1‑139 (MQ=255)

                              ggggtgggtggTTTATTGCTTATAAATGGGGTGCCGGTGAGGGTGGGTCGCGGGGTATCATTGCAGCCCGGGGCCCAGTTGGTACCCCCTCCCGTTTCGTTTTTATCTACACGACGGGGAGTCAGGCAACTATGGATGa                                                                                                             <  8:219964/135‑1 (MQ=255)

                              gggctggcgGGTTTTTTGCTGATAAATGTGGAGCGGGTGAGCGTGGGGCTCGGGGTATCATTGCAGCACTGGGGCCAGTTGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGa                                                                                                             <  1:5742/138‑1 (MQ=255)

                              cGGCTGGCTGTTTTATTGCTCATAAATCGGCCGCCGGTTAGCGGGGGTCTCGCGGAAAAATAGCACCTCGGGGGCCAGATGGAATGCCAACCTGTATGTTTTTAATCAACACGACGGGGAGTCAGGCAACTATGGATGa                                                                                                             <  8:26233/139‑1 (MQ=255)

                              cGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCGCGCGTTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGa                                                                                                             <  7:41005/139‑1 (MQ=255)

                                gctggctgGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTCAGCCCTCCCGTATCGTAGTTATCTACACGCCGGGGAGTCAGGCAACTATGGATGAAc                                                                                                           >  3:22691/1‑139 (MQ=255)

                                    gctggTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGaaa                                                                                                       >  4:9364/1‑139 (MQ=255)

                                           tATTGCTTATAATTCTGTAGCGGGTGAGGGGGGGTCTCGCGTTATCATTGCAGCAGTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATATACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACa                                                                                                 <  3:131356/138‑1 (MQ=255)

                                                   aTAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                                          >  4:240623/1‑137 (MQ=255)

                                                   aTAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                                          >  7:232651/1‑137 (MQ=255)

                                                   aTAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCATCCCGTATCGTAGTTATCTACACGAAGGGGAGTCAGGCACCTATGGATGAACGAAATAGAAAGAACGCTga                                                                                        >  7:102444/1‑139 (MQ=255)

                                                      aagcTGGAGCGGGTGAGCGGGGGTCTCGCGGTTTCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTTTCGTTGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGAt                                                                                     <  6:110901/136‑1 (MQ=255)

                                                         cTGGCGCCGGTGCGGGGGGGCCGCGCGGTATCATTGCAGCACTGGGGCCTGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAgg                                                                                  <  6:254949/139‑1 (MQ=255)

                                                              gCCGGTGCGCGGGGGTCCCGCTTTAACATTCCAGAACTGGGCCCTGTTGTTAACCCCTCCTTAATCTTTTTTTTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCt                                                                             <  8:108514/139‑1 (MQ=255)

                                                                gggggcgCGGGGGGCTCGCGGTATCTTTGCAGCAGGGGGGCCAGTTGTTAAGCCCTCCCGTATCTTAGTAATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                                           <  4:143312/133‑1 (MQ=255)

                                                                gggTGCGCGTGGGGATCGCGGTATCATTGCCCCAGTGGCCCCAGATGTTAAGCCCTCCCGTAGCGTAGTTATCTACACGACGGGGAGTCAGGCAAATATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                                           <  7:91146/138‑1 (MQ=255)

                                                                  gagAGCGTGGGTCTCGCGGAATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATATACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGCCAGATCGATGAGATAGGTGCCTCACt                                                                         >  8:186206/3‑139 (MQ=255)

                                                                  gTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACt                                                                         >  1:151771/1‑139 (MQ=255)

                                                                  gTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACt                                                                         >  5:196851/1‑139 (MQ=255)

                                                                  gTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCGCACt                                                                         >  5:150301/1‑139 (MQ=255)

                                                                      gggTGGGGCGCGGGTTATAATTGCCGCAGGGGGGCCATATGGTACGCCCTCCGGTATCGTAGTTTACTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGAtt                                                                     <  7:142337/137‑1 (MQ=255)

                                                                      gCGGGGGTCTCGCGTTATCATTGCCGCACGGGGGCCATATGGTACGCCCTCCCGTAGCGTAGTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGAtt                                                                     <  7:269562/139‑1 (MQ=255)

                                                                       ggTGGGTCGCGCGTTATATTGGCAGCAGTGGGGCCTTTTGTTAAGCCCGCCGGTATGGTTGTTATATACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGAtt                                                                     <  5:256665/137‑1 (MQ=255)

                                                                         tGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATAGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAg                                                                  >  8:35086/1‑139 (MQ=255)

                                                                         agggTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACCCGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAg                                                                  >  5:235009/2‑139 (MQ=255)

                                                                            gTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCAt                                                               >  3:39513/1‑139 (MQ=255)

                                                                                           tGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAc                                                >  1:42497/1‑139 (MQ=255)

                                                                                             cagcaCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAcc                                               >  2:4843/1‑138 (MQ=255)

                                                                                               gcaCTGGGGCCAGATGGTAAGCCCTCCCGTTTGGTTGGTTTCTTCCCGGCGGGGAGGAAGGCCACTTTGGATGCACGAAATAGACAGGTCGCTGAGATAGGTTCCGGCCTGCTTAAGCATTGGTAACTTGCAGGCCAAg                                            >  2:109772/1‑139 (MQ=255)

                                                                                                 ctgggggCCATATGGTACGCCCTCCGTTATTGTTTTTATCTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                                          <  1:277617/136‑1 (MQ=255)

                                                                                                 ccgggggCCAGATGGTACGCCCTCCCTTATCGTATTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                                          <  8:133196/136‑1 (MQ=255)

                                                                                                   tGGGGCCAGTTGTTACCCCCGCCCGTATCGTTTTTATCTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGAGCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTAAGACCAAGTTTa                                        <  6:211348/139‑1 (MQ=255)

                                                                                                   gggggCCAGATGTACAGCCCTCCCGCATCGTAGTAATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        <  6:59047/138‑1 (MQ=255)

                                                                                                   gggggCACGATGGTAAGCCCTCCCGTATCGTAGTTATAAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        <  5:50859/138‑1 (MQ=255)

                                                                                                     gggCCTGTTGGAAAGCCCTCCCGTATCGTATTTATCTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        <  8:46221/137‑1 (MQ=255)

                                                                                                     gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTTGCAGACCAAGTTTACt                                      >  6:230846/1‑139 (MQ=255)

                                                                                                     gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt                                      >  4:284595/1‑139 (MQ=255)

                                                                                                     gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt                                      >  1:188029/1‑139 (MQ=255)

                                                                                                     gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt                                      >  5:29764/1‑139 (MQ=255)

                                                                                                       gCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                                           <  4:167495/100‑1 (MQ=255)

                                                                                                       gCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCa                                    >  7:55376/1‑139 (MQ=255)

                                                                                                          aGATGTTAAGCCCTCCCGTATCGTAGTTATCTACAAGACGGGGATTCCGGCAACTATGGAGGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCTGACAAAGTTTACTCa                                    >  6:77532/1‑136 (MQ=255)

                                                                                                          aGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAc                                                <  7:159114/124‑1 (MQ=255)

                                                                                                          aGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGAGTAAGCATTGGTAACTGTCAGACCAAGTTTAGTCata                                  >  6:183758/1‑138 (MQ=255)

                                                                                                            aTGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                                          <  7:207950/128‑1 (MQ=255)

                                                                                                               gTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTAAGGATGACCGAAATAGACAGCTCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACt                            >  3:187594/1‑139 (MQ=255)

                                                                                                                tAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACTAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACtt                           >  2:256228/1‑139 (MQ=255)

                                                                                                                 aaGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGAATTGGTAACTGTCAGACCAAGTTTACTCATATATACtt                           >  4:51116/1‑138 (MQ=255)

                                                                                                                        cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATgg                                                                                                                 >  1:170322/1‑45 (MQ=255)

                                                                                                                        cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCAGTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgattga                   >  6:14725/1‑139 (MQ=255)

                                                                                                                        cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                                          >  4:127061/1‑116 (MQ=255)

                                                                                                                        cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgattga                   >  6:12970/1‑139 (MQ=255)

                                                                                                                        cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgattga                   >  5:110977/1‑139 (MQ=255)

                                                                                                                        cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgattga                   >  2:13825/1‑139 (MQ=255)

                                                                                                                        cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgattga                   >  1:83610/1‑139 (MQ=255)

                                                                                                                        cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgattga                   >  4:110450/1‑139 (MQ=255)

                                                                                                                         ccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgattgat                  >  5:132609/1‑139 (MQ=255)

                                                                                                                              tCGTAGTTATCTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgat                      <  3:60951/130‑1 (MQ=255)

                                                                                                                                 tAGTTATCTACACGACGTGGAGTCAGGCAACTATGGATGAACGAATTAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCATACCAAGTTTACTCATATATACTTTAGATTGATTTaaaaat          >  7:121996/1‑137 (MQ=255)

                                                                                                                                 tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                                          >  5:276677/1‑59 (MQ=255)

                                                                                                                                 tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGAtt                                                                     <  5:99894/80‑1 (MQ=255)

                                                                                                                                 tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGc                                                                 >  3:115109/1‑84 (MQ=255)

                                                                                                                                 tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAc                                                >  2:94115/1‑101 (MQ=255)

                                                                                                                                 tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgat                      <  7:178613/127‑1 (MQ=255)

                                                                                                                                 tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgat                      <  2:22414/127‑1 (MQ=255)

                                                                                                                                 tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTaaaaat          >  2:6885/1‑137 (MQ=255)

                                                                                                                                 tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACt          >  8:168667/1‑139 (MQ=255)

                                                                                                                                 tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACt          >  3:166168/1‑139 (MQ=255)

                                                                                                                                  atttATCTTCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGATGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACt          <  8:81363/136‑1 (MQ=255)

                                                                                                                                  aGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACtt         >  7:29391/1‑139 (MQ=255)

                                                                                                                                   gTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAcc                                               <  3:86367/100‑1 (MQ=255)

                                                                                                                                   gTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACtt         >  1:2120/1‑138 (MQ=255)

                                                                                                                                    ttATCTACACGACGGGTAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCa       >  4:75967/1‑139 (MQ=255)

                                                                                                                                      aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGt                                                           >  5:170126/1‑85 (MQ=255)

                                                                                                                                      aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGt                                                           <  2:283470/85‑1 (MQ=255)

                                                                                                                                      aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                                          <  2:17689/102‑1 (MQ=255)

                                                                                                                                      aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatat                                 >  5:74277/1‑111 (MQ=255)

                                                                                                                                      aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgat                      <  8:152474/122‑1 (MQ=255)

                                                                                                                                      aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTa               <  8:198592/129‑1 (MQ=255)

                                                                                                                                      aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCAtt     >  3:205516/1‑139 (MQ=255)

                                                                                                                                      aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCAtt     >  1:232291/1‑139 (MQ=255)

                                                                                                                                      aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCAtt     >  8:59220/1‑139 (MQ=255)

                                                                                                                                       tCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCAtt     <  3:145205/138‑1 (MQ=255)

                                                                                                                                         tACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGAt                                                                                     <  8:238163/56‑1 (MQ=255)

                                                                                                                                         tACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGAtt                                                                     >  2:197973/1‑72 (MQ=255)

                                                                                                                                         tACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCAtttt   >  7:71854/1‑138 (MQ=255)

TTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTT  >  pSIJ663_pHT315_araE_xy/4511‑4786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`