Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 1,603,312 C→A 9.1% S770S (TCC→TCA)  U712_08240 → Calcium‑transporting ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068811,603,3120CA 9.1% 132.6 / 3.4 44S770S (TCC→TCA) U712_08240Calcium‑transporting ATPase
Reads supporting (aligned to +/- strand):  ref base C (20/20);  new base A (2/2);  total (22/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.48e-01

GACGGACGGATTGCCGGCAATGGCGCTCGGCATGGATCAGCCGGAAGGAGACGTCATGAAGCGCAAGCCCCGTCATCCGAAAGAAGGGGTATTTGCCCGCAAGCTAGGATGGAAGGTCGTTTCCAGAGGATTTTTAATCGGAGTTGCCACTATTTTAGCGTTTATCATTGTGTACCACCGCAATCCGGAAAACTTGGCTTATGCCCAGACGATCGCGTTTGCCACATTGGTGCTCGCTCAGCTTATTCACG  >  CP006881/1603189‑1603439
                                                                                                                           |                                                                                                                               
gacggacgGATTGCCGGCAATGGCGCTCGGCATGGATCAGCCGGAAGGAGACGTCATGAAGCGCAAGCCCCGTCATCCGAAAGAAGGGGTATTTGCCCGCAAGCTAGGATGGAAGGTCGTTTCCAGAGGATTTTTAATc                                                                                                                  >  4:187043/1‑139 (MQ=255)
    gacggaTTGCCGGCAATGGCGCTCGGCATGGATCAGCCGGAAGGAGACGTCATGAAGCGCAAGCCCCGTCATCCGAAAGAAGGGGTATTTGCCCGCAAGCTAGGATGGAAGGTCGTTTCCAGAGGATTTTTAATCGGAg                                                                                                              >  8:158836/1‑139 (MQ=255)
        gaTTGCCGGCAATGGCGCTCGGCATGGATCAGCCGGAAGGAGACGTCATGAAGCGCAAGCCCCGTCATCCGAAAGAAGGGGTATTTGCCCGCAAGCTAGGATGGAAGGTCGTTTCCAGAGGATTTTTAATCGGAGTTGc                                                                                                          <  6:74370/139‑1 (MQ=255)
        gaTTGCCGGCAATGGCGCTCGGCATGGATCAGCCGGAAGGAGACGTCATGAAGCGCAAGCCCCGTCATCCGAAAGAAGGGGTATTTGCCCGCAAGCTAGGATGGAAGGTCGTTTCCAGAGGATTTTTAATCGGAGTTGc                                                                                                          <  8:230729/139‑1 (MQ=255)
           tGCCGGCAATGGGGCTCGGCATGGATCTGCCGTAAGGAGAAGTCATGAAGCGCTAGCGCCGTCATCCGAAAGAAGGGGTATTTGCCCGCAAGCTAGGATGGAAGGTCGTTTCCAGAGGATTTTTAATCGGAGTTGCCa                                                                                                        <  4:11504/138‑1 (MQ=255)
                            ggCATGGATCAGCCGGAAGGAGACGTCATGAAGCGCAAGCCCCGTCATCCGAAAGAAGGGGTATTTGCCCGCAAGCTAGGATGGAAGGTCGTTTCCAGAGGATTTTTAATCGGAGTTGCCACTATTTTAGCGTTTAt                                                                                        <  2:220605/137‑1 (MQ=255)
                            ggCATGGATCAGCCGGAAGGAGACGTCATGAAGCGCAAGCCCCGTCATCCGAAAGAAGGGGTATTTGCCCGCAAGCTAGGATGGAAGGTCGTTTCCAGAGGATTTTTAATCGGAGTTGCCACTATTTTAGCGTTTATCa                                                                                      <  7:43075/139‑1 (MQ=255)
                              cATGGATCAGCCGGAAGGAGACGTCATGAAGCGCAAGCCCCGTCATCCGAAAGAAGGGGTATTTGCCCGCAAGCTAGGATGGAAGGTCGTTTCCAGAGGATTTTTAATCGGAGTTGCCACTATTTTAGCGTTTATCAtt                                                                                    <  3:187043/139‑1 (MQ=255)
                                             aGGAGACGTCATGAAGCGCAAGCCCCGTCATCCGAAAGAAGGGGTATTTGCCCGCAAGCTAGGATGGAAGGTCGTTTCCAGAGGATTTTTAATc                                                                                                                  <  7:68114/94‑1 (MQ=255)
                                             aGGAGACGTCATGAAGCGCAAGCCCCGTCATCCGAAAGAAGGGGTATTTGCCCGCAAGCTAGGATGGAAGGTCGTTTCCAGAGGATTTTTAATc                                                                                                                  >  8:68114/1‑94 (MQ=255)
                                             aGGAGACGTCATGAAGCGCAAGCCCCGTCATCCGAAAGAAGGGGTATTTGCCCGCAAGCTAGGATGGAAGGTCGTTTCCAGAGGATTTTTAATCGGAGTTGCCACTATTTTAGCGTTTa                                                                                         <  1:198221/119‑1 (MQ=255)
                                             aGGAGACGTCATGAAGCGCAAGCCCCGTCATCCGAAAGAAGGGGTATTTGCCCGCAAGCTAGGATGGAAGGTCGTTTCCAGAGGATTTTTAATCGGAGTTGCCACTATTTTAGCGTTTa                                                                                         >  2:198221/1‑119 (MQ=255)
                                             aGGAGACGTCATGAAGCGCAAGCCCCGTCATCCGAAAGAAGGGGTATTTGCCCGCAAGCTAGGATGGAAGGTCGTTTCCAGAGGATTTTTAATCGGAGTTGCCACTATTTTAGCGTTTATCATTGTGTACCACCGCAAt                                                                     >  3:191437/1‑139 (MQ=255)
                                             aGGAGACGTCATGAAGCGCAAGCCCCGTCATCCGAAAGAAGGGGTATTTGCCCGCAAGCTAGGATGGAAGGTCGTTTCCAGAGGATTTTTAATCGGAGTTGCCACTATTTTAGCGTTTATCATTGTGTACCACCGCAAt                                                                     >  7:118174/1‑139 (MQ=255)
                                                 gaCGTCATGAAGCGCAAGCCCCGTCATCCGAAAGAAGGGGTATTTGCCCGCAAGCTAGGATGGAAGGTCGTTTCCAGAGGATTTTTAATCGGAGTTGCCACTATTTTAGCGTTTATCATTGTGTACCACCGCAATCCgg                                                                 <  3:52865/139‑1 (MQ=255)
                                                  aCGTCATGAAGCGCAAGCCCCGTCATCCGAAAGAAGGGGTATTTGCCCGCAAGCTAGGATGGAAGGTCGTTTCCAGAGGATTTTTAATCGGAGTTGCCACTATTTTAGCGTTTATCATAGTGTACCACCTCAATCCGGa                                                                <  5:239131/139‑1 (MQ=255)
                                                         gAAGCGCAAGCCCCGTCATCCGAAAGAAGGGGTATTTGCCCGCAAGCTAGGATGGAAGGTCGTTTCCAGAGGATTTTTAATCGGAGTTGCCACTATTTTAGCGTTTATCATTGTGTACCACCGCAATCCGGAAAACTTg                                                         >  2:199409/1‑139 (MQ=255)
                                                            gcgcAAGCCCCGTCATCCGAAAGAAGGGGTATTTGCCCGCAAGCTAGGATGGAAGGTCGTTTCCAGAGGATTTTTAATCGGAGTTGCCACTATTTTAGCGTTTATCATTGTGTACCACCGCAATCCGGAAAACTTGGCt                                                      <  1:199409/139‑1 (MQ=255)
                                                               cAAGCCCCGTCATCCGAAAGAAGGGGTATTTGCCCGCAAGCTAGGATGGAAGGTCGTTTCCAGAGGATTTTTAATCGGAGTTGCCACTATTTTAGCGTTTATCATTGTGTCCCACCGCAATCCGGAAAACTTGGCTTAt                                                   >  8:47380/1‑139 (MQ=255)
                                                               cAAGCCCCGTCATCCGAAAGAAGGGGTATTTGCCCGCAAGCTAGGATGGAAGGTCGTTTCCAGAGGATTTTTAATCGGAGTTGCCACTATTTTAGCGTTTATCATTGTGTACCACCGCAATCCGGAAAAc                                                            <  2:28607/130‑1 (MQ=255)
                                                               cAAGCCCCGTCATCCGAAAGAAGGGGTATTTGCCCGCAAGCTAGGATGGAAGGTCGTTTCCAGAGGATTTTTAATCGGAGTTGCCACTATTTTAGCGTTTATCATTGTGTACCACCGCAATCCGGAAAACTTGGCTTAt                                                   >  3:248078/1‑139 (MQ=255)
                                                               cAAGCCCCGTCATCCGAAAGAAGGGGTATTTGCCCGCAAGCTAGGATGGAAGGTCGTTTCCAGAGGATTTTTAATCGGAGTTGCCACTATTTTAGCGTTTATCATTGTGTACCACCGCAATCCGGAAAACTTGGCTTAt                                                   >  4:271238/1‑139 (MQ=255)
                                                               cAAGCCCCGTCATCCGAAAGAAGGGGTATTTGCCCGCAAGCTAGGATGGAAGGTCGTTTCCAGAGGATTTTTAATCGGAGTTGCCAATATTTTAGCGTTTATCATTGTGTACCACCGCAATACGGAAAAc                                                            >  1:28607/1‑130 (MQ=255)
                                                                       gTCATCCGAAAGAAGGGGTATTTGCCCGCAAGCTAGGATGGAAGGTCGTTTCCAGAGGATTTTTAATCGGAGTTGCCACTATTTTAGCGTTTATCATTGTGTACCACCGCAATCCGGAAAACTTGGCTTATGCCCAGAc                                           >  5:102789/1‑139 (MQ=255)
                                                                       gTCATCCGAAAGAAGGGGTATTTGCCCGCAAGCTAGGATGGAAGGTCGTTTCAAGAGGATTTTTAATCGGAGTTGCCACTATTTTAGCGTTTATCATTGTGTACCACCGCAATCCGGAAAACtt                                                          <  2:80633/124‑1 (MQ=255)
                                                                       gTCATCCGAAAGAAGGGGTATTTGCCCGCAAGCTAGGATGGAAGGTCGTTTCAAGAGGATTTTTAATCGGAGTTGCCACTATTTTAGCGTTTATCATTGTGTACCACCGCAATCCGGAAAACtt                                                          >  1:76406/1‑124 (MQ=255)
                                                                       gTCATCCGAAAGAAGGGGTATTTGCCCGCAAGCTAGGATGGAAGGTCGTTTCAAGAGGATTTTTAATCGGAGTTGCCACTATTTTAGCGTTTATCATTGTGTACCACCGCAATCCGGAAAACtt                                                          >  1:80633/1‑124 (MQ=255)
                                                                       gTCATCCGAAAGAAGGGGTATTTGCCCGCAAGCTAGGATGGAAGGTCGTTTCAAGAGGATTTTTAATCGGAGTTGCCACTATTTTAGCGTTTATCATTGTGTACCACCGCAATCCGGAAAACtt                                                          <  2:76406/124‑1 (MQ=255)
                                                                              gAAAGAAGGGGTATTTGCCCGCAAGCTAGGATGGAAGGTCGTTTCCAGAGGATTTTTAATCGGAGTTGCCACTAttt                                                                                                  >  7:82135/1‑77 (MQ=255)
                                                                              gAAAGAAGGGGTATTTGCCCGCAAGCTAGGATGGAAGGTCGTTTCCAGAGGATTTTTAATCGGAGTTGCCACTAttt                                                                                                  <  8:82135/77‑1 (MQ=255)
                                                                                     ggggTATTTGCCCGCAAGCTAGGAGGGAAGGTCGTTTCCAGAGGATTTTTAATCGGAGTTGCCACTATTTTAGCGTTTATCATTGTGTACCACCGCAATCCGGAAAACTTGGCTTATGCCCAGACGATCGCGTTTGCca                             <  4:24556/139‑1 (MQ=255)
                                                                                             tGCCCGCAAGCTAGGATGGAAGGTCGTTTCCAGAGGATTTTTAATCGGAGTTGCCACTATTTTAGCGTTTATCATTGTGTACCACCGCAATCCGGAAAACTTGGCTTATGCCCAGACGATCGCGTTTGCCACATTGGTg                     >  3:253856/1‑139 (MQ=255)
                                                                                                 cGCAAGCTAGGATGGAAGGTCGTTTCCAGAGGATTTTTAATCGGAGTTGCCACTATTTTTGCGTTTATCATTGTGTACCCCCGCAATCCGGAAAACTTGGCTTATGCCCAGACGATCGCGTTTGCCACATTGGTgctcg                 >  7:50458/1‑139 (MQ=255)
                                                                                                 cGCAAGCTAGGATGGAAGGTCGTTTCCAGAGGATTTTTAATCGGAGTTGCCACTATTTTAGCGTTTATCATTGTGTACCACCGCAATCCGGAAAACTTGGCTTATGCCCAGACGATCGCGTTTGCCACATTGGTgctcg                 >  2:46536/1‑139 (MQ=255)
                                                                                                   cAAGCTAGGATGGAAGGTCGTTTCCAGAGGATTTTTAATCGGAGTTGCCACTATTTTAGCGTTTATCATTGTGTACCACCGCAATCCGGAAAACTTGGCTTATGCCCAGACGATCGCGTTTGCCACATTGGTgctcgct               <  4:191437/139‑1 (MQ=255)
                                                                                                   cAAGCTAGGATGGAAGGTCGTTTCCAGAGGATTTTTAATCGGAGTTGCCACTATTTTAGCGTTTATCATTGTGTACCACCGCAATCCGGAAAACTTGGCTTATGCCCAGACGATCGCGTTTGCCACATTGGTgctcgct               <  8:118174/139‑1 (MQ=255)
                                                                                                   cAAGCTAGGATGGAAGGTCGTTTCCAGAGGATTTTTAATCGGAGTTGCCACTATTTTAGCGTTTATCATTGTGTACCACCGCAATCCGGAAAACTTGGCTTATGCCCAGACGATCGCGTTTGCCACATTGGTgctcgct               >  3:202538/1‑139 (MQ=255)
                                                                                                   cAAGCTAGGATGGAAGGTCGTTTCCAGAGGATTTTTAATCGGAGTTGCCACTATTTTAGCGTTTATCATTGTGTACCACCGCAATCCGGAAAACTTGGCTTATGCCCAGACGATCGCGTTTGCCACATTGGTg                     >  5:9266/1‑133 (MQ=255)
                                                                                                   cAAGCTAGGATGGAAGGTCGTTTCCAGAGGATTTTTAATCGGAGTTGCCACTATTTTAGCGTTTATCATTGTGTACCACCGCAATCCGGAAAACTTGGCTTATGCCCAGACGATCGCGTTTGCCACATTGGTg                     <  6:9266/133‑1 (MQ=255)
                                                                                                   cAAGCTAGGATGGAAGGTCGTTTCCAGAGGATTTTTAATCGGAGTTGCCACTATTTTAGCGTTTATCATTGTGTAACCCCGCAATCCGGAAAACTTGGGTTATGCCCATACGCTCGCGTTTGCCACAATGGTgctcgcg               >  1:121663/1‑138 (MQ=255)
                                                                                                       cTAGGATGGAAGGTCGTTTCCAGAGGATTTTTAATCGGAGTTGCCACTATTTTAGCGTTTATCATTGTGTACCACCGCAATCCGGAAAACTTGGCTTATGCCCAGACGATCGCGTTTGCCACATTGGTGCTCGCTCAGc           <  4:202538/139‑1 (MQ=255)
                                                                                                       cTAGGATGGAAGGTCGTTTCCAGAGGATTTTTAATCGGAGTTGCCACTATTTTAGCGTTTATCATTGTGTACCACCGCAATCCGGAAAACTTGGCTTATGCCCAGACGATCGCGTTTGCCACATTGGTGCTCGCTCAGc           <  3:271238/139‑1 (MQ=255)
                                                                                                               gAAGGTCGTTTCCAGAGGATTTTTAATCGGAGTTGCCACTATTTTAGCGTTTATCATTGTGTACCACCGCAATCCGGAAAACTTGGCTTATGCCCAGACGATCGCGTTTGCCACATTGGTGCTCGCTCAGCTTATTCa    <  5:138840/138‑1 (MQ=255)
                                                                                                                aaGGTCGTTTCCAGAGGATTTTTAATCGGAGTTTCCACAATTTTAGCGTTTATCATTGTGTACCACAGCAATCCGGAAAACATGGCTTATGACCAGACGCTCGCGTTTGCCACATTGGTGGTCGCTCAGCTTATTcgcg  >  7:110080/1‑139 (MQ=255)
                                                                                                                           |                                                                                                                               
GACGGACGGATTGCCGGCAATGGCGCTCGGCATGGATCAGCCGGAAGGAGACGTCATGAAGCGCAAGCCCCGTCATCCGAAAGAAGGGGTATTTGCCCGCAAGCTAGGATGGAAGGTCGTTTCCAGAGGATTTTTAATCGGAGTTGCCACTATTTTAGCGTTTATCATTGTGTACCACCGCAATCCGGAAAACTTGGCTTATGCCCAGACGATCGCGTTTGCCACATTGGTGCTCGCTCAGCTTATTCACG  >  CP006881/1603189‑1603439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑