Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 1,634,454 T→G 14.3% N79T (AAC→ACC)  U712_08390 ← Uncharacterized protein ylqB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068811,634,4540TG14.3% 62.9 / 2.4 40N79T (AAC→ACC) U712_08390Uncharacterized protein ylqB
Reads supporting (aligned to +/- strand):  ref base T (14/20);  new base G (4/2);  total (18/22)
Fisher's exact test for biased strand distribution p-value = 3.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

GTAATACGTATAGTAACCAGCAGCTGGCCCCAAATACATTGGAACCTCAAATGTTCCGTTTGCATCAGTCGTTCCTGAAGCAGTTTGTGTTGTGTTTCCGACCTTCGTGTCCGCTTCAAATCTTACGGGCGCGTTTGGCACTGGCTGT‑CCGTTTTGGTCGAGTAATTTGCCGCTTACTGTAATATTGTACTTGACTCGCCAATATTGACCTTGTCCGTAATTGATTTTACCGTATACCCCTCCATCTGTGCTGATATTTGTGATTGAG  >  CP006881/1634321‑1634588
                                                                                                                                     |                                                                                                                                       
gtAATACGTATAGTAACCAGCAGCTGGCCCCAAATACATTGGAACCTCAAATGTTCCGTTTGCATCAGTCGTTCCTGAAGCAGTTTGTGTTGTGTTTCCGACCTTCGTGTCCGCTTCAAATCTTACGGGCGCGGTTGGc                                                                                                                                    >  3:164102/1‑139 (MQ=255)
 tAATACGTATAGTAACCAGCAGCTGGCCCCAAATACATTGGAACCTCAAATGTTCCGTTTGCATCAGTCGTTCCTGAAGCAGTTTGTGTTGTGTTTCCGACCTTCGTGTCCGCTTCAAATCTTACGGGCGCGTTTGGCa                                                                                                                                   <  8:99174/139‑1 (MQ=255)
      cGTATAGTAACCAGCAGCTGGCCCCAAATACATTGGAACCTCAAATGTTCCGTTTGCATCAGTCGTTCATGAAGCAGTTTGTGTTGTGTTTCCGACCTTCGTGTCCGCTTCAAATCTTACGGGCGCGTTTGGCACTggg                                                                                                                              >  5:64319/1‑138 (MQ=255)
        tataGTAACCAGCAGCTGGCCCCAAATACATTGGAACCTCAAATGTTCCGTTTGCATCAGTCGTTCCTGAAGCAGTTTGTGTTGTGTTTCCGACCTTCGTGTCCGCTTCAAATCTTACGGGCGCGTTTGGCACTGGCTg                                                                                                                            >  6:271167/1‑139 (MQ=255)
         ataGTAACCAGCAGCTGGCCCCAAATACATTGGAACCTCAAATGTTCCGTTTGCATCAGTCGTTCCTGAAGCAGTTTGTGTTGTGTTTCCGACCTTCGTGTCCGCTTCAAATCTTACGGGCGCGTTTGGCCCTGGCTGt                                                                                                                           >  7:41520/1‑139 (MQ=255)
               aCCAGCAGCTGGCCCCAAATACATTGGAACCTCAAATGTTCCGTTTGCATCAGTCGTTCCTGAAGCAGTTTGTGTTGTGTTTCCGACCTTCGTGTCCGCTTCAAATCTTACTGGCGCGTTTGGGACGGCCTGT‑CCGttt                                                                                                                    >  6:125369/1‑139 (MQ=255)
               aCCAGCAGCTGGCCCCAAATACATTGGAACCTCAAATGTTCCGTTTGCATCAGTCGTTCCTGAAGCAGTTTGTGTTGTGTTTCCGACCTTCGTGTCCGCTTCAAATCTTACGGGCGCGTTTGGCACTGGCTGT‑CCGttt                                                                                                                    >  1:34368/1‑139 (MQ=255)
                    cagcTGGCCCCAAATACATTGGAACCTCAAATGTTCCGTTTGCATCAGTCGTTCCTGAAGCAGTTTGTGTTGTTTTTCCGACCTTCGTGTCCGCTTCAAATCTTACGGGCGCGGTTGGCACTGGCTGT‑GCGTGTTTGtg                                                                                                               >  7:75182/1‑138 (MQ=255)
                    cagcTGGCCCCAAATACATTGGAACCTCAAATGTTCCGTTTGCATCAGTCGTTCCTGAAGCAGTTTGTGTTGTGTTTCCGACCTTCGTGTCCGCTTCAAATCTTACGGGCGCGTTTGGCGCGGGCTGT‑GCGGTTTGGTc                                                                                                               >  6:265848/1‑139 (MQ=255)
                       cTGGCCCCAAATACATTGGAACCTCAAATGTTCCGTTTGCATCAGTCGTTCCTGAAGCAGTTTGTGTTGTGTTTCCGACCTTCGTGTCCGCTTCAAATCTTACGGGCGCGTTTGGCACGGGCTGT‑CCGTTTTGGTCGAg                                                                                                            >  3:90080/1‑139 (MQ=255)
                           ccccAAATACATTGGAACCTCAAATGTTCCGTTTGCATCAGTCGTTCCTGAAGCAGTTTGTGTTGTGTTTCCGACCTTCGTGTCCGCTTCAAATCTTACGGGCGCGTTTGGCACTGGCTGT‑CCGTTTTGGTCGAGTAAt                                                                                                        >  5:114640/1‑139 (MQ=255)
                                   aCATTGGAACCTCAAATGTTCCGTTTGCATCAGTCGTTCCTGAAGCAGTTTGTGTTGTGTTTCCGACCTTCGTGTCCGCTTCAAATCTTACGGGCGCGTTTGGCACTGGCTGT‑CCGTTTTGGTCGAGTAATTTGCCGCt                                                                                                <  6:75336/139‑1 (MQ=255)
                                    cATTGGAACCTCAAATGTTCCGTTTGCATCAGTCGTTCCTGAAGCAGTTTGTGTTGTGTTTCCGACCTTCGTGTCCGCTTCAAATCTTACGGGCGCGTTTGGCACTGGCTGT‑CCGTTTTGGTCGAGTAATTTGCCGCt                                                                                                <  5:125369/138‑1 (MQ=255)
                                    cATTGGAACCTCAAATGTTCCGTTTGCATCAGTCGTTCCTGAAGCAGTTTGTGTTGTGTTTCCGACCTTCGTGTCCGCTTCAAATCTTACGGGCGCGTTTGGCACTGGCTGT‑CCGTTTTGGTCGAGTAATTTGCCGCt                                                                                                <  6:178968/138‑1 (MQ=255)
                                     aTTGGAACCTCAAATGTTCCGTTTGCATCAGTCGTTCCTGAAGCAGTTTGTGTTGTGTTTCCGACCTTCGTGTCCGCTTCAAATCTTACGGGCGCGTTTGGCACTGGCTGT‑CCGTTTTGGTCGAGTAATTTGCCGCTTa                                                                                              <  6:25230/139‑1 (MQ=255)
                                         gAACCTCAAATGTTCCGTTTGCATCAGTCGTTCCTGAAGCAGTTTGTGTTGTGTTTCCGACCTTCGTGTCCGCTTCAAATCTTACGGGCGCGTTTGGCACTGGCTGT‑CCGTTTTGGTCGAGTAATTTGCCGCTTACTg                                                                                           <  3:164123/138‑1 (MQ=255)
                                               cAAATGTTCCGTTTGCATCAGTCGTTCCTGAAGCAGTTTGTGTTGTGTTTCCGACCTTCGTGTCCGCTTCAAATCTTACGGGCGCGGTTGGCACTGGCTGT‑CCGTTTTGGTAGAGTAATTTGCCGCTTACTGTTATAtt                                                                                    >  3:285487/1‑139 (MQ=255)
                                                 aaTGTTCCGTTTGCATCAGTCGTTCCTGAAGCAGTTTGTGTTGTGTTTCCGACCTTCGTGTCCGCTTCAAATCTTACGGGCGCGTTTGGCCCGGGCGGG‑CCGTTTTGGTCGAGTAATTTGCCGCTTACTGTAATATTGt                                                                                  >  8:4799/1‑139 (MQ=255)
                                                  aTGTTCCGTTTGCATCAGTCGTTACTGAAGGAGGTTGTGTTGTGTTTCCGACGTTCGTGTCCGCTTCAAATCTTCCGGGCGCGTTTGGCACTGGCGGT‑CCGTTTTGGTCGAGTAATTTGCCGCTTACTGTAATATTGTa                                                                                 <  7:123701/139‑1 (MQ=255)
                                                       ccGTTTGCATCAGTCGTTCCTGAAGCAGTTTGTGTTGTGTTTCCGACCTTCGTGTCCGCTTCAAATCTTACGGGCGCGTTTGGCACTGGCTGT‑CCGTTTTGGTCGAGTAATTTGCCGCTTACTGTAATATTGTACTTGa                                                                            >  7:89469/1‑139 (MQ=255)
                                                       ccGTTTGCATCAGTCGTTCCTGAAGCAGTTTGTGTTGTGTTTCCGACCTGCGTGTCCGCTTCAAATCTTACGGGCGCGTTTGGCACTGGCTGT‑CCGTTTTGGTCGAGTAATTTGCCGCTTACTGTAATATTGTACTTGa                                                                            <  6:100348/139‑1 (MQ=255)
                                                        cGTTTGCATCAGTCGTTCCTGAAGCAGTTTGTGTTGTGTTTCCGACCTTCGTGTCCGCTTCAAATCTTACGGGCGCGTTTGGCACTGGCTGT‑CCGTTTTGGTCGAGTAATTTGCCGCTTACTGTAATATTGTACTTGa                                                                            <  3:41605/138‑1 (MQ=255)
                                                          tttGCATCAGTCGTTCCTGAAGCAGTTTGTGTTGTGTTTCCGACCTTCGTGTCCGCTTCAAATCTTACGGGCGCGTTTGGGCCGGGCTGT‑CCGTTTTTGTCGAGTAATTTTCGGCTTTGTGGAATATTTTAATTGaggg                                                                         >  3:154699/1‑136 (MQ=255)
                                                             gCATCAGTCGTTCCTGAAGCAGTTTGTGTTGTGTTTCCGACCTTCGTGTCCGCTTCAAATCTTACGGGCGCGTTTGGCACTGGCTGT‑CCGTTTTGGTCGAGTAATTTGCCGCTTACTGTAATATTGTACTTGACTCGc                                                                       <  6:114640/138‑1 (MQ=255)
                                                                        tCCTGAAGCAGTTTGTGTTGTGTTTCCGACCTTCGTGTCCGCTTCAAATCTTACGGGCGCGTTTTGCACTGGCTGT‑CCGTTTTGGGGGAGTTATTTGCCGCTTACTGTAATATTTTACTTGAATCGCCAATATTGAccg                                                           >  7:165290/1‑138 (MQ=255)
                                                                         ccTGAAGCAGTTTGTGTTGTGTTTCCGACCTTCGTGTCCGCTTCAAATCTTACGGGCGCGTTTGGCACTGGCTGT‑CCGTTTTGGTCGAGTAATTTGCCGc                                                                                                 <  8:48117/100‑1 (MQ=255)
                                                                         ccTGAAGCAGTTTGTGTTGTGTTTCCGACCTTCGTGTCCGCTTCAAATCTTACGGGCGCGTTTGGCACTGGCTGT‑CCGTTTTGGTCGAGTAATTTGCCGc                                                                                                 >  7:48117/1‑100 (MQ=255)
                                                                             aaGCAGTTTGTGTTGTGTTTCCGACCTTCGTGTCCGCTTCAAATCTTACGGGCGCGGTTGGCACTGGCTGT‑CCGTTTTGGTCGAGTAATTTGCCGCTTACTGTAATATTGTACTTGACTCGCCAATATTGACCTTGTc                                                       <  5:97550/138‑1 (MQ=255)
                                                                                         ttgtgttTCCGACCTTCGTGTCCGCTTCAAATCTTACGGGCGCGTTTGGCACTGGCTGT‑CCGTTTTGGTCGAGTAATTTGCCGCTTACTGTAATATTGTACTTGACTCGCCAATATTGACCTTGTCCGTAATTGAttt                                           <  5:131972/138‑1 (MQ=255)
                                                                                          tggggttCCGACCTTCGTGTCCGCTTCAAATCTTACGGGCGCGTTTGGCACTGGCTGT‑CCGTTTTGGTCGAGTAATTTGCCGCTTACTGTAATATTGTACTTGACTCGCCAATATTGACCTTGTCCGTAATTGATTTTa                                         <  4:151762/134‑1 (MQ=255)
                                                                                              tttCCGACCTTCGTGTCCGCTTCAAATCTTACGGGCGCGTTTGGCACTGGCTGT‑CCGTTTTGGTCGAGTAATTTGCCGCTTTCTGTAATATTGTTCTTGACTCGCCAATATTGACCTTGTCCGTTATTGATTTTACCGt                                     >  3:116610/1‑139 (MQ=255)
                                                                                                 ccGACCTTCGTGTCCGCTTCAAATCTTACGGGCGCGTTTGGCACTGGCTGT‑CCGTTTTGGTCGAGTAATTTGCCGCTTACTGTAATATTGTACTTGACTCGCCAATATTGACCTTGTCCGTAATTGATTTTACCGtat                                   <  2:34368/138‑1 (MQ=255)
                                                                                                 ccGACCTTCGTGTCCGCTTCAAATCTTACGGGCGCGTTTGGCACTGGCTGT‑CCGTTTTGGTCGAGTAATTTGCCGCTTACTGTAATATTGTACTTGACTCGCCAATATTGACCTTGTCCGTAATTGATTTTACCGtat                                   <  5:271167/138‑1 (MQ=255)
                                                                                                     ccTTCGTGTCCGCTTCAAATCTTACGGGCGCGGTTGGCACTGGCTGT‑CCGTTTTGGTCGAGTAATTTGCCGCTTACTGTAATATTGTACTTGACTCGCCAATATTGACCTTGTCCGTAATTGATTTTACCGTATAcccc                              <  4:164102/139‑1 (MQ=255)
                                                                                                          gtgtGCGCTTCAAATCTTACGGGCGCGTTTGGCACTGGCTGT‑CCGTTTTGGTCGAGTAATTTGCCGCTTACTGTAATATTGTACTTGACTCGCCAATATTGACCTTGTCCGTAATTGATTTTACCGTATACCCCTCCAt                         <  8:75182/139‑1 (MQ=255)
                                                                                                          gtgtCCGCTTCAAATCTTACGGGCGCGTTTGGCACTGGCTGT‑CCGTTTTGGTCGAGTAATTTGCCGCTTACTGTAATATTGTACTTGACTCGCCAATATTGACCTTGTCCGTAATTGATTTTACCGTATACCCCTCCAt                         <  8:89469/139‑1 (MQ=255)
                                                                                                            gtCCGCTTCAAATCTTACGGGCGCGTTTGGCACTGGCTGT‑CCGTTTTGGTCGAGTAATTTGCCGCTTACTGTAATATTGTACTTGACTCGCCAATATTGACCTTGTCCGTAATTGATTTTACCGTATACCCCTCCATCt                       <  4:90080/139‑1 (MQ=255)
                                                                                                               cGCTTCAAATCTTACGGGCGCGGTTTGCACTGGCGGTACCGTTTT‑GTCGAGTTATTTGCCGCATAAAGTAATATTTTAAATGACAAGCCAAAAATTAACATGTGCGTAAATTATATTAACGGTTACCCCGCCaacagtt                    >  2:19811/1‑133 (MQ=255)
                                                                                                                                 cgcgTTTGGCACTGGCTGT‑CCGTTTTGGTCGAGTAATTTGCCGCTTACTGTAATATTGTACTTGACTCGCCAATATTGACCTTGTCCGTAATTGATTTTACCGTATACCCCTCCATCTGTGCTGATATTTGTGATTGAg  <  5:265848/139‑1 (MQ=255)
                                                                                                                                 cgcgTTTGGCACTGGCTGT‑CCGTTTTGGTCGAGTAATTTGCCGCTTACTGTAATATTGTACTTGACTCGCCAATATTGACCTTGTCCGTAATTGATTTTACCGTATACCCCTCCATCTGTGCTGATATTTGTGATTGAg  <  8:41520/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                       
GTAATACGTATAGTAACCAGCAGCTGGCCCCAAATACATTGGAACCTCAAATGTTCCGTTTGCATCAGTCGTTCCTGAAGCAGTTTGTGTTGTGTTTCCGACCTTCGTGTCCGCTTCAAATCTTACGGGCGCGTTTGGCACTGGCTGT‑CCGTTTTGGTCGAGTAATTTGCCGCTTACTGTAATATTGTACTTGACTCGCCAATATTGACCTTGTCCGTAATTGATTTTACCGTATACCCCTCCATCTGTGCTGATATTTGTGATTGAG  >  CP006881/1634321‑1634588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑