Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 1,701,244 G→A 7.7% A125T (GCA→ACA)  U712_08750 → Riboflavin biosynthesis protein ribC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068811,701,2440GA 7.7% 205.4 / 5.6 65A125T (GCA→ACA) U712_08750Riboflavin biosynthesis protein ribC
Reads supporting (aligned to +/- strand):  ref base G (32/28);  new base A (3/2);  total (35/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.19e-01

AAGACAAAATAAACCAAATTGAACAATTAGGCACAGAAGTTCTGTATGTCGTTAAATTTAATGAAGTGTTTGCTTCTCTTTCTCCTAAGCAGTTTATAGACCAGTATATTATCGGCCTTAATGTGCAGCACGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTGGGTGCACAATGGTAGAAAAATTAACGGAGCAGGATAAAAAAATCAGTTCTTCGTAT  >  CP006881/1701107‑1701378
                                                                                                                                         |                                                                                                                                      
aaGACAAAATAAACCAAATTGAACAATTAGGCACAGAAGTTCTGTATGTCGTTAAATTTAATGAAGTGTTTGCTTCTCTTTCTCCTAAGCAGTTTATAGACCAGTATATTATCGGCCTTAATGTGCAGCACGCAGTgg                                                                                                                                        <  3:201101/138‑1 (MQ=255)
       aaTAAACCAAATTGAACAATTAGGCACAGAAGTTCTGTATGTCGTTAAATTTAATGAAGTGTTTGCTTCTCTTTCTCCTAAGCAGTTTATAGACCAGTATATTATCGGCCTTAATGTGCAGCACGCAGTGGCAGGCttt                                                                                                                                >  6:260505/1‑139 (MQ=255)
                aaTTGAACAATTAGGCACAGAAGTTCTGTATGTCGTTAAATTTAATGAAGTGTTTGCTTCTCTTTCTCCTAAGCAGTTTATAGACCAGTATATTATCGGCCTTAATGTGCAGCCCGCAGTGGCAGGCTTTGACTTtacg                                                                                                                       >  8:5428/1‑139 (MQ=255)
                aaTTGAACAATTAGGCACAGAAGTTCTGTATGTCGTTAAATTTAATGAAGTGTTTGCTTCTCTTTCTCCTAAGCAGTTTATAGACCAGTATATTATCGGCCTTAATGTGCAGCACGCAGTGGCAGGCTTTGACTTtacg                                                                                                                       >  8:1288/1‑139 (MQ=255)
                 aTTGAACAATTAGGCACAGAAGTTCTGTATGTCGTTAAATTTAATGAAGTGTTTGCTTCTCTTTGTCCTAAGCAGTTTATAGACCAGTATATTATCGGCCTTAATGTGCAGCACGCAGTGGCAGGCTTTGACTTtacg                                                                                                                       >  1:129525/1‑138 (MQ=255)
                 aTTGAACAATTAGGCACAGAAGTTCTGTATGTCGTTAAATTTAATGAAGTGTTTGCTTCTCTTTCTCCTAAGCAGTTTATAGACCAGTATATTATCGGCCTTAATGTGCAGCACGCAGTGGCAGGCTTTGACTTtacgt                                                                                                                      <  7:125951/139‑1 (MQ=255)
                          ttAGGCACAGAAGTTCTGTATGTCGTTAAATTTAATGAAGTGTTTGCTTCTCTTTCTCCTAAGCAGTTTATAGACCAGTATATTATCGGCCTTAATGTGCAGCACGCAGTGGCAGGCTTTGACTTTACGTACGGCaaa                                                                                                              >  7:201349/1‑138 (MQ=255)
                                     aGTTCTGTATGTCGTTAAATTTAATGAAGTGTTTGCTTCTCTTTCTCCTAAGCAGTTTATAGACCAGTATATTATCGGCCTTAATGTGCAGCACGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGa                                                                                                  <  8:201349/139‑1 (MQ=255)
                                     aGTTCTGTATGTCGTTAAATTTAATGAAGTGTTTGCTTCTCTTTCTCCTAAGCAGTTTATAGACCAGTATATTATCGGCCTTAATGTGCAGCACGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGa                                                                                                  <  5:260505/139‑1 (MQ=255)
                                       ttCTGTATGTCGTTAAATTTAATGAAGTGTTTGCTTCTCTTTCTCCTAAGCAGTTTATAGACCAGTATATTATCGGCCTTAATGTGCAGCACGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGaa                                                                                                 <  2:129525/138‑1 (MQ=255)
                                              tGTCGTTAAATTTAATGAAGTGTTTGCTTCTCTTTCTCCTAAGCAGTTTATAGACCAGTATATTATCGGCCTTAATGTGCAGCACGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCa                                                                                                       <  2:9184/125‑1 (MQ=255)
                                              tGTCGTTAAATTTAATGAAGTGTTTGCTTCTCTTTCTCCTAAGCAGTTTATAGACCAGTATATTATCGGCCTTAATGTGCAGCACGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCa                                                                                                       >  1:9184/1‑125 (MQ=255)
                                                tCGTTAAATTTAATGAAGTGTTTGCTTCTCTTTCTCCTAAGCAGTTTATAGACCAGTATATTATCGGCCTTAATGTGCAGCACGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGAc                                                                                       <  7:164591/139‑1 (MQ=255)
                                                   ttAAATTTAATGAAGTGTTTGCTTCTCTTTCTCCTAAGCAGTTTATAGACCAGTATATTATAGGCCTTAATGTGCAGCACGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCAt                                                                                    >  3:85116/1‑139 (MQ=255)
                                                                  tgtTTGCTTCTCTTTCTCCTAAGCAGTTTATAGACCAGTATATTATCGGCCTTAATGTGCAGCACGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACAATGCCGGATGATTTAGa                                                                     >  1:173809/1‑139 (MQ=255)
                                                                                tctcCTAAGCAGTTTATAGACCAGTATATTATCGGCCTTAATGTGCAGCACGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTGGGt                                                       >  4:224368/1‑139 (MQ=255)
                                                                                           gTTTATAGACCAGTATATTATCGGCCTTAATGTGCAGCACGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTGGGTGCACAATGGTa                                            <  7:235201/139‑1 (MQ=255)
                                                                                           gTTTATAGACCAGTATATTATCGGCCTTAATGTGCAGCACGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTGGGTGCACAATGGTa                                            <  7:2499/139‑1 (MQ=255)
                                                                                            tttATAGACCAGTATATTATCGGCCTTAATGTGCAGCACGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTGGGTGCACAATgg                                              >  1:206196/1‑136 (MQ=255)
                                                                                                      aGTATATTATCGGCCTTAATGTGCAGCACGCAGTGGc                                                                                                                                       >  7:216987/1‑37 (MQ=255)
                                                                                                      aGTATATTATCGGCCTTAATGTGCAGCACGCAGTGGc                                                                                                                                       <  8:216987/37‑1 (MQ=255)
                                                                                                      aGTATATTATCGGCCTTAATGTGCAGCACGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTGGGTGCACAATGGTAGAAAAATTAAc                                 >  8:170256/1‑139 (MQ=255)
                                                                                                        tataTTATCGGCCTTAATGTGCAGCACGCAGTGGCAGTCTTTGACTTTACGTACGGCAAATAAGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTGGTTGCACAATGTTAGAAAAATTAACg                                >  1:285764/1‑138 (MQ=255)
                                                                                                        tataTTATCGGCCTTAATGTGCAGCACGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTGGGTGCACAATGGTAGAAAAATTAACg                                >  1:88230/1‑138 (MQ=255)
                                                                                                          tattatCGGCCTTAATGTGCAGCACGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTGGGTGCACAATGGTAGAAAAATTAACGGAg                             <  8:148988/139‑1 (MQ=255)
                                                                                                            ttatCGGCCTTAATGTGCAGCCGGCAGTGGCGGGCTTTGAATTTACTTACGGCAAATACGGCAAGGGAACAATGAAGCCCAGCCGGGAGGTTTTAGACGGAAAAGCTGGGTGCACAATGGTAGAAAATTTacggcag                             >  2:160954/1‑131 (MQ=255)
                                                                                                             tatCGGCCTTAATGTGCAGCACGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTGGGTGCACAATGGTAGAAAAATTAACGGAGCAg                          >  2:221884/1‑139 (MQ=255)
                                                                                                               tCGGCCTTAATGTGCAGCACGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTGGGTGCACAATGGTAGAAAAATTAACGGAGCAGGa                        >  4:272082/1‑139 (MQ=255)
                                                                                                                  gCCTTAATGTGCAGCACGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAAGAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTGGGTGCACAATGGTAGAAAAATTAACGGAGCAGGATa                      >  2:137758/1‑138 (MQ=255)
                                                                                                                  gCCTTAATGTGCAGCACGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGa                                                                     <  7:11889/91‑1 (MQ=255)
                                                                                                                  gCCTTAATGTGCAGCACGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGa                                                                     >  8:11889/1‑91 (MQ=255)
                                                                                                                  gCCTTAATGTGCAGCACGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCt                                                           >  5:160365/1‑101 (MQ=255)
                                                                                                                  gCCTTAATGTGCAGCACGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTggg                                                        <  1:148919/104‑1 (MQ=255)
                                                                                                                  gCCTTAATGTGCAGCACGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTggg                                                        >  2:148919/1‑104 (MQ=255)
                                                                                                                  gCCTTAATGTGCAGCACGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTGGGTg                                                      >  8:24226/1‑106 (MQ=255)
                                                                                                                  gCCTTAATGTGCAGCACGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTGGGTg                                                      <  7:24226/106‑1 (MQ=255)
                                                                                                                  gCCTTAATGTGCAGCACGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTGGGTGCACAATGGTAGAAAAATTAACGGAGCAGGATa                      >  4:184166/1‑138 (MQ=255)
                                                                                                                  gCCTTAATGTGCAGCACGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTGGGTGCACAATGGTAGAAAAATTAACGGAGCAGGATa                      >  1:74247/1‑138 (MQ=255)
                                                                                                                  gCCTTAATGTGCAGCACGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTGGGTGCACAATGGTAGAAAAATTAACGGAGCAGGATa                      >  2:109730/1‑138 (MQ=255)
                                                                                                                  gCCTTAATGTGCAGCACGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTGGGTGCACAATGCTAGAAAAATTACCGGAGCAGGATa                      >  2:142622/1‑138 (MQ=255)
                                                                                                                  gCCTTAATGTGCAGCACGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGAAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCt                                                           <  6:160365/101‑1 (MQ=255)
                                                                                                                   ccTTATTGTGCAGCACGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTGGGTGCACAATGGTAGAAAAATTAACGGAGCAGGATaaa                    <  8:237967/139‑1 (MQ=255)
                                                                                                                   ccTTAATGTGCAGCACGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTGGGTGCACAATGGTAGAAAAATTAACGGAGCAGGATaaa                    <  3:224368/139‑1 (MQ=255)
                                                                                                                     ttAATGTGCAGCACGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTGGGTGCACAATGGTAGAAAAATTAACGGAGCAGGATaaaa                   >  7:246223/1‑138 (MQ=255)
                                                                                                                             cagcaCGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTGGGTGCACAATGGTAGAAAAATTAACGGAGCAGGATAAAAAAATCAGtt          <  1:186116/139‑1 (MQ=255)
                                                                                                                             cagcaCGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTGGGTGCACAATGGTAGAAAAATTAACGGAGCAGGATAAAAAAATCAGtt          >  2:186116/1‑139 (MQ=255)
                                                                                                                             cagcaCGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTGGGTGCACAATGGTAGAAAAATTAACGGAGCAGGATAAAAAAATCAGtt          >  8:182673/1‑139 (MQ=255)
                                                                                                                             cagcaCGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTGGGTGCACAATGGTAGAAAAATTAACGGAGCAGGATAAAAAAATCAGtt          <  2:206196/139‑1 (MQ=255)
                                                                                                                             cagcaCGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTGGGTGCACAATGGTAGAAAAATTAACGGAGCAGGATAAAAAAATCAGtt          <  2:88230/139‑1 (MQ=255)
                                                                                                                             cagcaCGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTGGGTGCACAATGGTAGAAAAATTAACGGAGCAGGATAAAAAAATCAGtt          <  2:74247/139‑1 (MQ=255)
                                                                                                                             cagcaCGCAGTGACAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTGGGTGCACAATGGTAGAAAAATTAACGGAGCAGGATAAAAAAATCAGtt          >  1:201445/1‑139 (MQ=255)
                                                                                                                             cagcaCGCAGTGACAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTGGGTGCACAATGGTAGAAAAATTAACGGAGCAGGATAAAAAAATCAGtt          >  1:205421/1‑139 (MQ=255)
                                                                                                                             cagcaCGCAGTGACAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTGGGTGCACAATGGTAGAAAAATTAACGGAGCAGGATAAAAAAATCAGtt          >  1:249481/1‑139 (MQ=255)
                                                                                                                              aaaggtcAGTGGCAGGCTTTTCCTATACGTAAGGAAAATACGGAAAGGTTACAATTAAGACCATGCCGTATTATTTAGACGGAAAAGCTTGGTGCACAATGGTAGAAAAATTAACGGAGCAGGATAAAAAAATCAGtt          <  6:155993/132‑1 (MQ=255)
                                                                                                                               gcaCGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTGGGTGCACAATGGTAGAAAAATTAACGGAGCAGGATAAAAAAATCAGttct        <  7:1288/139‑1 (MQ=255)
                                                                                                                               gcaCGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTGGGTGCACAATGGTAGAAAAATTAACGGAGCAGGATAAAAAAATCAGttct        <  7:5428/139‑1 (MQ=255)
                                                                                                                                caCGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTGGGTGCACAATGGTAGAAAAATTAACGGAGCAGGATAAAAAAATCAGttctt       >  1:192409/1‑139 (MQ=255)
                                                                                                                                  cGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTGGGTGCACAATGGTAGAAAAATTAACGGAg                             <  7:26333/115‑1 (MQ=255)
                                                                                                                                  cGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTGGGTGCACAATGGTAGAAAAATTAACGGAg                             >  8:26333/1‑115 (MQ=255)
                                                                                                                                  cGCAGGGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTGGGTGCACAATGGTAGAAAAATTAACGGAGCAGGATAAAAAAATCAGTTCTTCg     <  8:246223/139‑1 (MQ=255)
                                                                                                                                    cAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTGGGTGCACAATGGTAGAAAAATTAACGGAGCAGGATAAAAAAATCAGTTCTTCGta   <  2:192409/139‑1 (MQ=255)
                                                                                                                                    cAGTGACAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTGGGTGCACAATGGTAGAAAAATTAACGGAGCAGGATAAAAAAATCAGTTCTTCGta   <  2:205421/139‑1 (MQ=255)
                                                                                                                                    cAGTGACAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTGGGTGCACAATGGTAGAAAAATTAACGGAGCAGGATAAAAAAATCAGTTCTTCGta   <  2:201445/139‑1 (MQ=255)
                                                                                                                                     aGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGaa                                                                                                 >  1:210228/1‑44 (MQ=255)
                                                                                                                                     aGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGaa                                                                                                 <  2:210228/44‑1 (MQ=255)
                                                                                                                                     aGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTGGGTGCACAATGGTAGAAAAATTAACGGAGCAGGATAAAAAAATCAGTTCTTCGtat  <  7:182673/139‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                      
AAGACAAAATAAACCAAATTGAACAATTAGGCACAGAAGTTCTGTATGTCGTTAAATTTAATGAAGTGTTTGCTTCTCTTTCTCCTAAGCAGTTTATAGACCAGTATATTATCGGCCTTAATGTGCAGCACGCAGTGGCAGGCTTTGACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGACGGAAAAGCTGGGTGCACAATGGTAGAAAAATTAACGGAGCAGGATAAAAAAATCAGTTCTTCGTAT  >  CP006881/1701107‑1701378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑