Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 1,965,296 T→C 5.9% Q531R (CAG→CGG)  U712_09710 ← Glutamate synthase [NADPH] large chain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068811,965,2960TC 5.9% 211.6 / 2.8 68Q531R (CAG→CGG) U712_09710Glutamate synthase [NADPH] large chain
Reads supporting (aligned to +/- strand):  ref base T (37/27);  new base C (2/2);  total (39/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAACTGTTCATTGGATAAAACAGGTGTATACAGTTTAATCCGGCGAACATTTCGTTCACTTGGATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGCTGTTTAAAGTAGTTAAACAGCGATTGCGCTCGGTCAGAAAGTACAGCAAGCGGAGCGTCATTCCCCATTGAACCAAGGGGATCTTTGCCT  >  CP006881/1965181‑1965433
                                                                                                                   |                                                                                                                                         
aaaCTGTTCATTGGATAAAACAGGTGTATACAGTTTAATCCGGCGAACATTTCGTTCACTTGGATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGc                                                                                                                    <  3:142206/139‑1 (MQ=255)
aaaCTGTTCATTGGATAAAACAGGTGTATACAGTTTAATCCGGCGAACATTTCGTTCACTTGGATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGc                                                                                                                    <  8:263411/139‑1 (MQ=255)
      ttCATTGGATAAAACAGGTGTATACAGTTTAATCCGGCGAACATTTCGTTCACTTGGATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTc                                                                                                              >  6:123898/1‑139 (MQ=255)
      ttCATTGGATAAAACAGGTGTATACAGTTTAATCCGGCGAACATTTCGTTCACTTGGATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTc                                                                                                              >  7:200774/1‑139 (MQ=255)
      ttCATTGGATAAAACAGGTGTATACAGTTTAATCCGGCGAACATTTCGTTCACTTGGATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTc                                                                                                              >  3:254094/1‑139 (MQ=255)
      ttCATTGGATAAAACAGGTGTATACAGTTTAATCCGGCGAACATTTCGTTCACTTGGATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTc                                                                                                              >  2:187571/1‑139 (MQ=255)
      ttCATTGGATAAAACAGGTGTATACAGTTTAATCCGGCGAACATTTCGTTCACTTGGATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTc                                                                                                              >  3:124811/1‑139 (MQ=255)
      ttCATTGGATAAAACAGGTGTATACAGTTTAATCCGGCGAACATTTCGTTCACTTGGATGAAGAAGATCGCCTTACGCCCCGAGCCAAGTCATCGTTCAAGTGACAAGCTGCTCACGAATCGCGTCGAGAGGCTGGTTc                                                                                                              >  5:82100/1‑139 (MQ=255)
           tGGAAAATACAGGTGTATACAGTTTAATCCGGCGAACATTTCGTTGACTTGGATGAAGAAGCGCGCGTTCCGCACCGAGCCAAGTCTTCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTGCGt                                                                                                            <  6:82100/136‑1 (MQ=255)
                aaaaCAGGTGTATACAGTTTAATCCGGCGAACATTTCGTTCACTTGGATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTtgtg                                                                                                    >  7:185760/1‑139 (MQ=255)
                aaaaCAGGTGTATACAGTTTAATCCGGCGAACATTTCGTTCACTTGGATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTtgtg                                                                                                    >  7:189973/1‑139 (MQ=255)
                    cAGGTGTATACAGTATAATCCGGCGAACATTTCGTTCACTTGGATGAAGAAGAGCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCaaa                                                                                                <  4:46474/139‑1 (MQ=255)
                       gtgtATACAGTTTAATCCGGCGAACATTTCGTTCACTTGGATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAATCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAg                                                                                             >  4:4365/1‑139 (MQ=255)
                       gtgtATACAGTTTAATCCGGCGAACATTTCGTTCACTTGGATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAg                                                                                             >  7:168508/1‑139 (MQ=255)
                       gtgtATACAGTTTAATCCGGCGAACATTTCGTTCACTTGGATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAg                                                                                             <  3:73750/139‑1 (MQ=255)
                       gtgtATACAGTTTAATCCGGCGAACATTTCGTTCACTTGGATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAg                                                                                             >  3:257422/1‑139 (MQ=255)
                       gtgtATACAGTTTAATCCGGCGAACATTTCGTTCACTTGGATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAg                                                                                             <  5:202598/139‑1 (MQ=255)
                       gtgtATACAGTTTAATCCGGCGAACATTTCGTTCACTTGGATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTGCGTTACTTGTGCAAACAg                                                                                             <  5:125448/139‑1 (MQ=255)
                            taCAGTTTAATCCGGCGAACATTTCGTTCACTTGGATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGCTGtt                                                                                        >  6:273265/1‑139 (MQ=255)
                            taCAGTTTAATCCGGCGAACATTTCGTTCACTTGGATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGCTGtt                                                                                        >  3:209229/1‑139 (MQ=255)
                            taCAGTTTAATCCGGCGAACATTTCGTTCACTTGGATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGCTGtt                                                                                        >  4:161287/1‑139 (MQ=255)
                               aGTTTAATCCGGCGAACATTTCGTTCACTTGGATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGCTGTTTaa                                                                                     <  4:181422/139‑1 (MQ=255)
                                 tttAATCCGGCGAACATTTCGTTCACTTGGATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCa                                                                                                  >  3:191469/1‑124 (MQ=255)
                                 tttAATCCGGCGAACATTTCGTTCACTTGGATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCa                                                                                                  <  4:191469/124‑1 (MQ=255)
                                 tttAATCCGGCGAACATTTCGTTCACTTGGATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGCTGTTTAAag                                                                                   >  6:290567/1‑139 (MQ=255)
                                 tttAATCCGGCGAACATTTCGTTCACTTGGATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGCTGTTTAAag                                                                                   >  7:37401/1‑139 (MQ=255)
                                 tttAATCCGGCGAACATTTCGTTCACTTGGATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGCTGTTTAAag                                                                                   <  8:185760/139‑1 (MQ=255)
                                 tttAATCCGGCGAACATTTCGTTCACTTGGATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGCTGTTTAAag                                                                                   <  8:189973/139‑1 (MQ=255)
                                 tttAATCCGGCGAACATTTCGTTCACTTGGATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGCTGTTTAAag                                                                                   >  1:27127/1‑139 (MQ=255)
                                 tttAATCCGGCGAACATTTCGTTCACTTGGATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGCTGTTTAAag                                                                                   >  3:74977/1‑139 (MQ=255)
                                 tttAATCCGGCGAACATTTCGTTCACTTGGATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGCTGTTTAAag                                                                                   >  3:55271/1‑139 (MQ=255)
                                 tttAATCCGGCGAACATTTCGTTCACTTGGATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGCTGTTTAAag                                                                                   >  1:23098/1‑139 (MQ=255)
                                 tttAATCCGGCGAACATTTCGTTCACTTGGATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGATGTTTAAag                                                                                   >  7:15856/1‑139 (MQ=255)
                                  ttAATCCGGCGAACATTTCGTTCACTTGGATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGCTGTTTAAag                                                                                   <  4:55271/138‑1 (MQ=255)
                                    aaTCCGGCGAACATTTCGTTCACTTGGATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGCTGTTTAAagtag                                                                                >  3:231268/1‑139 (MQ=255)
                                      tCCGGCGAACATTTTGTTCACTTGGATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGCTGTTTAAagtagt                                                                               >  7:43928/1‑138 (MQ=255)
                                      tCCGGCGAACATTTCGTTCACTTGGATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTGCGTTACTTGTGCAAACAGCTGTTTAAAGTAGtt                                                                              >  1:262660/1‑139 (MQ=255)
                                           cGAACATTTCGTTCACTTGGATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGCTGTTTAAAGTAGTTaaa                                                                           >  3:60952/1‑137 (MQ=255)
                                           cGAACATTTCGTTCACTTGGATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGCTGTTTAAAGTAGTTaaa                                                                           >  6:99619/1‑137 (MQ=255)
                                                      ttCACTTGGATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGCTGTTTAAAGTAGTTAAACAGCGATTGCGCt                                                              <  4:124811/139‑1 (MQ=255)
                                                      ttCACTTGGATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGCTGTTTAAAGTAGTTAAACAGCGATTGCGCt                                                              <  8:200774/139‑1 (MQ=255)
                                                          cTTGGATGAAGAAGATCGCCTTCCGCACCTAGCCAAGTCATCGTTGAAGTGACAATCCGCTCACGAATCGCGTCGCTTGGCGTGTTCTTTACTTGTGCAAAAAGCGGTTTAAAGTAGTTAAACAGCTCTTGCtctctg                                                           >  6:202752/1‑136 (MQ=255)
                                                          cTTGGATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCCGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGCTGTTTAAAGTAGTTAAACAGCGATTGCGCTCGGt                                                          >  6:206805/1‑139 (MQ=255)
                                                              gATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGCTGTTTAAAGTAGTTAAACAGCGATTGCGCTCGGTCAGa                                                      <  5:99619/139‑1 (MQ=255)
                                                              gATGAAGAAGATCGCCTTCCGCACCGAGCCAAGGCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGGCGATTGGCGGGTTCGTTACTTGTGCAAACAGCTGTTTAAAGTAGTTAAACAGCGATTGCGCTCGGTCAGa                                                      <  2:117043/139‑1 (MQ=255)
                                                                   agaagaTCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTtgt                                                                                                     <  6:160972/87‑1 (MQ=255)
                                                                   agaagaTCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTtgt                                                                                                     >  5:160972/1‑87 (MQ=255)
                                                                     aagaTCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGCTGTTTAAAGTAGTTAAACAGCGATTGCGCTCGGTCAGAAAGTa                                                 >  3:244099/1‑137 (MQ=255)
                                                                           gCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGCTGTTTAAAGTAGTTAAACAGCGATTGCGCTCGGTCAGAAAGTACAGCAAg                                          <  3:4365/138‑1 (MQ=255)
                                                                            ccTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGCTGTTTAAag                                                                                   >  7:243639/1‑96 (MQ=255)
                                                                            ccTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGCTGTTTAAag                                                                                   <  8:243639/96‑1 (MQ=255)
                                                                            ccTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGCTGTTTAAAGTAGTTAAACAGCGATTGCGCTCGGTCAGAAAGTACAGCAAGCg                                        <  3:103272/139‑1 (MQ=255)
                                                                            ccTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGCTGTTTAAAGTAGTTAAACAGCGATTGCGCTCGGTCAGAAAGTACAGCAAGCg                                        <  7:139379/139‑1 (MQ=255)
                                                                             cTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGCTGTTTAAAGTAGTTAAACAGCGATTGCGCTCGGTCAGAAAGTACAGCAAGCgg                                       <  3:161287/139‑1 (MQ=255)
                                                                               tCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAg                                                                                             <  4:237181/83‑1 (MQ=255)
                                                                               tCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAg                                                                                             >  3:237181/1‑83 (MQ=255)
                                                                                ccGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGCTGTTTAAAGTAGTTAAACAGCGATTGCGCTCGGTCAGAAAGTACAGCAAGCGGAg                                     <  8:15856/138‑1 (MQ=255)
                                                                                       gAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGCTGTTTAAAGTAGTTAAACAGCGATTGCGCTCGGTCAGAAAGTACAGCAAGCGGAGCGTCATTc                             >  8:147159/1‑139 (MQ=255)
                                                                                        aGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGCTGTTTAAAGTAGTTAAACAGCGATTGCGCTCGGTCAGAAAGTACAGCAAGCGGCGCGTCATTcc                            >  8:125242/1‑139 (MQ=255)
                                                                                           cAAGTCATCGTTGAAGTGACCAGCCGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGCTGTTTAAAGTAGTTAAACAGCGATTGCGCTCGGTCAGAAAGTACAGCAAGCGGAGCGTCATTCCCCa                         <  5:206805/139‑1 (MQ=255)
                                                                                           cAAGTCATCGTTGAAGTGACCAGCCGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGATGTTTAAAGTATTTAAACAGCGATTGCGCTCGGTAAGAAAGTACAGCAAGCGGAGCGTCATTCCCCa                         <  5:202752/139‑1 (MQ=255)
                                                                                                   cGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAACCAGCTGTTTAAAGTAGTTAAACAGCGATTGCGCTCGGTCAGAAAGTACAGCAAGCGGAGCGTCATTCCCCATTGAAcc                  >  4:215546/1‑138 (MQ=255)
                                                                                                   cGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGGTCGTTACGTGTGCAAACAGCTGTTTAAAGTAGTTAAACAGCGATTGCGCGCGGTCATAAAGTACCGCAAGCGGAGCGGCCTTGCCCATTGCAcc                  >  2:148918/1‑138 (MQ=255)
                                                                                                     ctGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTGCGTTACTTGTGCAAACAGCTGTTTAAAGTAGTTAAACAGCGATTGCGCTCGGTCAGAAAGTACAGCAAGCGGAGCGTCATTCCCCATTGAACCAAg               <  1:27546/138‑1 (MQ=255)
                                                                                                       gAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGCTGTTTAAAGTAGTTAAACAGCGATTGCGCTCGGTCAGAAAGTACAGCAAGCGGAGCGTCATTCCCCATTGAACCAAggg             >  7:160536/1‑139 (MQ=255)
                                                                                                         aGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGCTGTTTAAAGTAGTTAAACAGCGATTGCGCTCGGTCAGAAAGTACAGCAAGCGGAGCGTCATTCCCCATTGAACCAAGGGGa           <  8:43928/139‑1 (MQ=255)
                                                                                                             aCAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGCTGTTTAAAGTAGTTAAACAGCGATTGCGCTCGGTCAGAAAGTACAGCAAGCGGAGCGTCATTCCCCATTGAACCAAGGGGATCtt       >  4:279212/1‑139 (MQ=255)
                                                                                                               aaGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGCTGTTTAAAGTAGTTAAACAGCGATTGCGCTCGGTCAGAAAGTACAGCAAGCGGAGCGTCATTCCCCATTGAACCAAGGGGATCTTTg     <  2:262660/139‑1 (MQ=255)
                                                                                                                 gctgctCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGCTGTTTAAAGTAGTTAAACAGCGATTGCGCTCGGTCAGAAAGTACAGCAAGCGGAGCGTCATTCCCCATTGAACCAAGGGGATCTTTGcc   <  5:123898/139‑1 (MQ=255)
                                                                                                                  ctgctCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGCTGTTTAAAGTAGTTAAACAGCGATTGCGCTCGGTCAGAAAGTACAGCAAGCGGAGCGTCATTCCCCATTGAACCAAGGGGATCTTTGCct  >  2:87541/1‑139 (MQ=255)
                                                                                                                   |                                                                                                                                         
AAACTGTTCATTGGATAAAACAGGTGTATACAGTTTAATCCGGCGAACATTTCGTTCACTTGGATGAAGAAGATCGCCTTCCGCACCGAGCCAAGTCATCGTTGAAGTGACAAGCTGCTCACGAATCGCGTCGATTGGCGGGTTCGTTACTTGTGCAAACAGCTGTTTAAAGTAGTTAAACAGCGATTGCGCTCGGTCAGAAAGTACAGCAAGCGGAGCGTCATTCCCCATTGAACCAAGGGGATCTTTGCCT  >  CP006881/1965181‑1965433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑