| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 2,301,541 | T→C | 6.1% | I28I (ATT→ATC) | U712_11635 → | Uncharacterized protein yqzJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 2,301,541 | 0 | T | C | 6.1% | 200.7 / 3.6 | 65 | I28I (ATT→ATC) | U712_11635 | Uncharacterized protein yqzJ |
| Reads supporting (aligned to +/- strand): ref base T (21/40); new base C (2/2); total (23/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.10e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01 | |||||||||||
ACAACAGCTTGTACATATAGTAAAATGAAGCTATTGAATGGATGTGATGACAATGTTTGTGGAATCGATAAATGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCGTGCGGGCGAGCAATGGAAATCAAAACGAACAGATGAACATCAGACGGTCTTTCACATTAACCGAACAGACTTTCTTATTATTATATATCATCGCATTACAACTTGGATA > CP006881/2301406‑2301673 | acaacaGCTTGTACATATAGTAAAATGAAGCTATTGAATGGATGTGATGACAATGTTTGTGGAATCGATAAATGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCa < 4:112408/139‑1 (MQ=255)acaacaGCTTGTACATATAGTAAAATGAAGCTATTGAATGGATGTGATGACAATGTTTGTGGAATCGATAAATGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCa < 1:124382/139‑1 (MQ=255) aacaGCTTGTACATATAGTAAAATGAAGCTATTGAATGGATGTGATGACAATGTTTGTGGAATCGATAAATGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCAtt < 8:266403/139‑1 (MQ=255) aacaGCTTGTACATATAGTAAAATGAAGCTATTGAATGGATGTGATGACAATGTTTGTGGAATCGATAAATGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCAtt < 8:124026/139‑1 (MQ=255) aacaGCTTGTACATATAGTAAAATGAAGCTATTGAATGGATGTGATGACAATGTTTGTGGAATCGATAAATGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCAtt < 4:271833/139‑1 (MQ=255) aacaGCTTGTACATATAGTAAAATGAAGCTATTGAATGGATGTGATGACAATGTTTGTGGAATCGATAAATGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCAtt < 2:3435/139‑1 (MQ=255) aacaGCTTGTACATATAGTAAAATGAAGCTATTGAATGGATGTGATGACAATGTTTGTGGAATCGATAAATGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCAtt > 6:136937/1‑139 (MQ=255) caGCTTGTACATATAGTAAAATGAAGCTATTGAATGGATGTGATGACAATGTTTGTGGAATCGATAAATGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTcc < 8:17517/139‑1 (MQ=255) cTTGTACATATAGTAAAATGAAGCTATTGAATGGATGTGATGACAATGTTTGTGGAATCGATAAATGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCctct < 1:163372/139‑1 (MQ=255) cTTGTACATATAGTAAAATGAAGCTATTGAATGGATGTGATGACAATGTTTGTGGAATCGATAAATGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCctct < 2:88959/139‑1 (MQ=255) cTTGTACATATAGTAAAATGAAGCTATTGAATGGATGTGATGACAATGTTTGTGGAATCGATAAATGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCctct < 8:208516/139‑1 (MQ=255) cTTGTACATATAGTAAAATGAAGCTATTGAATGGATGTGATGACAATGTTTGTGGAATCGATAAATGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCctct < 8:204679/139‑1 (MQ=255) cTTGTACATATAGTAAAATGAAGCTATTGAATGGATGTGATGACAATGTTTGTGGAATCGATAAATGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCctct > 7:208516/1‑139 (MQ=255) cTTGTACATATAGTAAAATGAAGCTATTGAATGGATGTGATGACAATGTTTGTGGAATCGATAAATGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCctct > 7:204679/1‑139 (MQ=255) cATATAGTAAAATGAAGCTATTGAATGGATGTGATGACAATGTTTGTGGAATCGATAAATGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCCTCTTACgg < 1:46048/138‑1 (MQ=255) taGTAAAATGAAGCTATTGAATGGATGTGATGACAATGTTTGTGGAATCGATAAATGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTg < 1:152079/139‑1 (MQ=255) aaaaTGAAGCTATTGAATGGATGTGATGACAATGTTTGTGGAATCGATAAATGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCg < 4:255520/138‑1 (MQ=255) aaaaTGAAGCTATTGAATGGATGTGATGACAATGTTTGTGGAATCGATAAATGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCGt > 7:84192/1‑139 (MQ=255) aaaaTGAAGCTATTGAATGGATGTGATGACAATGTTTGTGGAATCGATAAATGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCGt > 1:240603/1‑139 (MQ=255) aaaTGAAGCTATTGAATGGATGTGATGACAATGTTTGTGGAATCGATAAATGACGGTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCGTg < 3:90866/139‑1 (MQ=255) aGCTATTGAATGGATGTGATGACAATGTTTGTGGAATCGATAAATGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCAtt < 5:99007/113‑1 (MQ=255) aGCTATTGAATGGATGTGATGACAATGTTTGTGGAATCGATAAATGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCAtt > 6:99007/1‑113 (MQ=255) aGCTATTGAATGGATGTGATGACAATGTTTGTGGAATCGATAAATGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCGTGCGGGc > 2:256586/1‑138 (MQ=255) cTATTGAATGGATGTGATGACAATGTTTGTGGAATCGATAAATGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCGTGCGGGCGAg > 6:154324/1‑139 (MQ=255) aaTGGATGTGATGACAATGTTTGTGGAATCGATAAATGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCGTGCGGGCGAGCAATgg < 4:5469/139‑1 (MQ=255) gATGTGATGACAATGTTTGTGGAATCGATAAATGACGTTTTATTCTTAGTCGATTTTTTCCCAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCGGGCGGGCGAGCAATGGCAAt > 5:7024/1‑139 (MQ=255) atgaCAATGTTTGTGGAATCGATAAATGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCGTg < 8:227515/115‑1 (MQ=255) atgaCAATGTTTGTGGAATCGATAAATGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCGTg > 7:227515/1‑115 (MQ=255) aCAATGTTTGTGGAATCGATAAATGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCGTGCGGGCGAGCAATGGAAATCAAAACGaa < 2:170568/139‑1 (MQ=255) cAATGTTTGTGGAATCGATAAATGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCGTGCGGGCGAGCAATGGAAATCAAAACGaa < 5:108960/138‑1 (MQ=255) aaTGTTTGTGGAATCGATAAATGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATCGCATTCCTCTTACGGGAGTGCCGTGCGGGCGAGCAATGGAAATCAAAACGaa > 6:277119/1‑137 (MQ=255) aaTGTTTGTGGAATCGATAAATGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATCGCATTCCTCTTACGGGAGTGCCGTGCGGGCGAGCAATGGAAATCAAAACGaa > 6:272955/1‑137 (MQ=255) tGTTTGTGGAATCGATAAATGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCGTGCGGGCGAGCAATGGAAATCAAAACGAACAGa < 2:240603/139‑1 (MQ=255) gTTTGTGGAATCGATAAATGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCGTGCGGGCGAGCAATGGAAATCAAAACGAACAGAt > 2:55161/1‑139 (MQ=255) tgGAATCGATAAATGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCGTGCGGGCGAGCAATGGAAATCAAAACGAACAGAt > 1:246355/1‑134 (MQ=255) tgGAATCGATAAATGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCGTGCGGGCGAGCAATGGAAATCAAAACGAACAGAt < 2:246355/134‑1 (MQ=255) tCGATAAATGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCGTGCGGGCGAGCAATGGAAATCAAAACGAACAGATGAACATCAGa < 3:88010/139‑1 (MQ=255) tCGATAAATGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCGTGCGGGCGAGCAATGGAAATCAAAACGAACAGATGAACATCAGa < 1:256586/139‑1 (MQ=255) tCGATAAATGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCGTGCGGGCGAGCAATGGAAATCAAAACGAACAGATGAACATCAGa < 1:280669/139‑1 (MQ=255) tCGATAAATGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCGTGCGGGCGAGCAATGGAAATCAAAACGAACAGATGAACATCAGa < 3:227862/139‑1 (MQ=255) gATAAATGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCGTGCGGGCGAGCAATGGAAATCAAAACGAACAGATGAACATCAGAc < 5:155537/138‑1 (MQ=255) aTGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCGTGCGGGCGAGCAATGGAAATCAAAACGAACAGATGAACATCAGACGGTCtt < 2:181110/139‑1 (MQ=255) aCGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCGTGCGGGCGAGCAATGGAAATCAAAACGAACAGATGAACATCAGAc > 1:9219/1‑130 (MQ=255) aCGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCGTGCGGGCGAGCAATGGAAATCAAAACGAACAGATGAACATCAGAc < 2:9219/130‑1 (MQ=255) ttttATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCGTGCGGGCGAGCAATGGAAATCAAAACGAACAGATGAACATCAGACGGTCTTTCACAt > 5:110296/1‑139 (MQ=255) aTTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCCTATTACGTGAGTGCCGTGCGTGCGATCAATGGATATCAAAACGAACAGATGAACATCAGACGGTCTTTCACATTaa < 4:145630/138‑1 (MQ=255) aGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCGTGCGGGCGAGCAATGGAAATCAAAACGAACAGATGAACATCAGACGGTCTTTCACATTAACCGaa > 5:55261/1‑137 (MQ=255) aGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCGTGCGGGCGAGCAATGGAAATCAAAACGAACAGATGAACATCAGACGGTCTTTCACATTAACCGaa < 6:110296/137‑1 (MQ=255) aGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCAATCCTCTTACGGGAGTGCCGTGCGGGCGAGCAATGGAAATCAAAACGAACAGATGAACATCAGACGGTCTTTCACATTAACCGaa > 3:191535/1‑137 (MQ=255) gTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCGTGCGGGCGAGCAATGGAAATCAAAACGAACAGATGAACATCAGACGGTCTTTCACATTAACCGAACa < 8:26056/138‑1 (MQ=255) gATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCGTGCGGGCGAGCAATGGAAATCAAAACGAACAGATGAACATCAGACGGTCTTTCACATTAACCGAACAGAc < 3:197924/138‑1 (MQ=255) aTTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCGTGCGGGCGAGCAATGGAAATCAAAACGAACAGATGAACATCAGACGGTCTTTCACATTAACCGAACAGACtt > 2:81478/1‑139 (MQ=255) tttCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCGTGCGGGCGAGCAATGGAAATCAAAACGAACAGATGAACATCAGACGGTCTTTCACATTAACCGAACAGACTTTCt < 8:84192/138‑1 (MQ=255) tttCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATCGCATTCCTCTTACGGGAGTGCCGTGCGGGCGAGCAATGGAAATCAAAACGAACAGATGAACATCAGACGGTCTTTCACATTAACCGAACAGACTTTCt < 5:277119/138‑1 (MQ=255) tttCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATCGCATTCCTCTTACGGGAGTGCCGTGCGGGCGAGCAATGGAAATCAAAACGAACAGATGAACATCAGACGGTCTTTCACATTAACCGAACAGACTTTCt < 5:272955/138‑1 (MQ=255) cacaATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCGTGCGGGCGAGCAATGGAAATCAAAACGAACAGATGAACATCAGACGGTCTTTCACATTAACCGAACAGACTTTCttat < 5:154324/138‑1 (MQ=255) acaATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCGTGCGGGCGAGCAATGGAAATCAAAACGAACAGATGAACATCAGACGGTCTTTCACATTAACCGAACAGACTTTCttatta < 7:1555/139‑1 (MQ=255) attattCTTCCTGCTCTAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCGTGCGGGCGAGCAATGGAAATCAAAACGAACAGATGAACATCAGACGGTCTTTCACATTAACCGAACAGACTTTCttattatta < 2:183235/139‑1 (MQ=255) ccTGCTCTAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCGTGCGGGCGAGCAATGGAAATCAAAACGAACAGATGAACATCAGACGGTCTTTCACATTAACCGAACAGACTTTCTTATTATTATATatcat < 1:55161/138‑1 (MQ=255) tGCTCTAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCGTGCGGGCGAGCAATGGAAATCAAAACGAACAGATGAACATCAGACGGTCTTTCACATTAACCGAACAGACTTTCTTATTATTATATATCATCg > 1:152402/1‑138 (MQ=255) gCTCTAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCGTGCGGGCGAGCAATGGAAATCAAAACGAACAGATGAACATCAGACGGTCTTTCACATTAACCGAACAGACTTTCtta < 6:232034/121‑1 (MQ=255) gCTCTAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCGTGCGGGCGAGCAATGGAAATCAAAACGAACAGATGAACATCAGACGGTCTTTCACATTAACCGAACAGACTTTCtta > 5:232034/1‑121 (MQ=255) ctctAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCGTGCGGGCGAGCAATGGAAATCAAAACGAACAGATGAACATCAGACGGTCTTTCACATTAACCGAACAGACTTTCtta < 6:183105/120‑1 (MQ=255) ctctAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCGTGCGGGCGAGCAATGGAAATCAAAACGAACAGATGAACATCAGACGGTCTTTCACATTAACCGAACAGACTTTCtta > 5:183105/1‑120 (MQ=255) cGGGATTGCATTCCTCTTACGGGAGTGCCGTGCGGGCGAGCAATGGAAATCAAAACGAACAGATGACCATCAGACGGTCTTTCACATTAACCGAACAGACTTTCTTATTATTATATATCATCGCATTACAACTTGGATa > 2:10774/1‑139 (MQ=255) | ACAACAGCTTGTACATATAGTAAAATGAAGCTATTGAATGGATGTGATGACAATGTTTGTGGAATCGATAAATGACGTTTTATTCTTAGTCGATTTTTTCACAATTATTCTTCCTGCTCTAACGGCAATCGGGATTGCATTCCTCTTACGGGAGTGCCGTGCGGGCGAGCAATGGAAATCAAAACGAACAGATGAACATCAGACGGTCTTTCACATTAACCGAACAGACTTTCTTATTATTATATATCATCGCATTACAACTTGGATA > CP006881/2301406‑2301673 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |