Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 2,304,097 T→G 27.4% T189P (ACC→CCC)  U712_11650 ← Uncharacterized protein yqjE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,304,0970TG27.4% 36.4 / 5.7 32T189P (ACC→CCC) U712_11650Uncharacterized protein yqjE
Reads supporting (aligned to +/- strand):  ref base T (4/19);  new base G (7/2);  total (11/21)
Fisher's exact test for biased strand distribution p-value = 2.60e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTGTGCCGCCTTCAAACCGGCCGATATTAGCTGTTGTTTCTTCATCGATACGGCCCAATGGCATTTTTGAGATGGCTTTGCTAGCGATTGTAATCGCTGAAATGCCTTTTTCAGGTTCAACCCCGGCGTGAGCGGTTTTCCCGAAAATTGCCGCTCTTACCTTCGCCTGTGTCGGCGCGGCCACAATGATATTTCCGACTTTTCCGTCTGAATCAAGAGCATAGCCGTAGGATGCAGTGATCATAGAACGGT  >  CP006881/2303961‑2304213
                                                                                                                                        |                                                                                                                    
tgtgtgCCGCCTTCAAACCGGCCGATATTAGCTGTTGTTTCTTCATCGATACGGCCCAATGGCATTTTTGAGATGGCTTTGCTAGCGATTGTAATCGCTGAAATGCCTTTTTCAGGTTCAACCCAGGCGTGGGGGGttt                                                                                                                    >  2:207286/1‑139 (MQ=255)
  tgtgCCGCCTTCAAACCGGCCGATATTAGCTGTTGTTTCTTCATCGATACGGCCCAATGGCATTTTTGAGATGGCTTTGCTAGCGATTGTAATCGCTGAAATGCCTTTTTCAGGTTCAACCCCGGCGTGGGGGGgtttg                                                                                                                  >  5:180936/1‑138 (MQ=255)
       cgccTTCACACCGGCCGATATTAGCTGTTGTTTCTTCATCGATACGGCCCAATGGCATTTTTGAGATGGCTTTGCTAGCGATTGTAATCGCTGAAATGCCTTTTTCAGGTTCAACCCCGGCGTGAGCGGTTTTCCCGaa                                                                                                             <  1:74031/139‑1 (MQ=255)
        gccTTCAAACCGGCCGATATTAGCTGTTGTTTCTTCATCGATACGGCCCAATGGCATTTTTGAGATGGCTTTGCTAGCGATTGTAATCGCTGAAATGCCTTTTTCAGGTTCAACCCCGGCGTGAGCGGGTTTGCCGaa                                                                                                             >  1:178046/1‑138 (MQ=255)
             cAAACCGGCCGATATTAGCTGTTGTTTCTTCATCGATACGGCCCAATGGCATTTTTGAGATGGCTTTGCTAGCGATTGTAATCGCTGAAATGCCTTTTTCAGGTTCAACCCCGGCGTGAGCGGTTTTCCCGAAAATTg                                                                                                        <  1:8715/138‑1 (MQ=255)
             cAAACCGGCCGATATTAGCTGTTGTTTCTTCATCGATACGGCCCAATGGCATTTTTGAGATGGCTTTGCTAGCGATTGTAATCGCTGAAATGCCTTTTTCAGGTTCAACCCCGGCGTGAGCGGTTTTCCCGAAAATTg                                                                                                        <  3:157382/138‑1 (MQ=255)
               aaCCGGCCGATATTAGCTGTTGTTTCTTCATCGATACGGCCCAATGGCATTTTTGAGATGGCTTTGCTAGCGATTGTAATCGCTGAAATGCCTTTTTCAGGTTCAACCCCGGCGTGAGCGGTTTTCCCGAAAATTGCCg                                                                                                     <  4:178912/139‑1 (MQ=255)
                  cGGCCGATATTAGCTGTTGTTTCTTCATCGATACGGCCCAATGGCATTTTTGAGATGGCTTTGCTAGCGATTGTAATCGCTGAAATGCCTTTTTCAGGTTCAACCCCGGCGTGAGCGGTTTTCCCGAAAATTGCCGctc                                                                                                  <  5:145393/139‑1 (MQ=255)
                  cGGCCGATATTAGCTGTTGTTTCTTCATCGATACGGCCCAATGGCATTTTTGAGATGGCTTTGCTAGCGATTGTAATCGCTGAAATGCCTTTTTCAGGTTCAACCCCGGCGTGAGCGGTTTTCCCGAAAATTGCCGctc                                                                                                  <  5:108120/139‑1 (MQ=255)
                   ggCCGATATTAGCTGTTGGTTCTTCATCGAGACGGCCCAATGGCATTTTTGAGATGGCATTGCTAGCGATTGTAATCGCTGAAATGCCTTTTTCAGGTTCAACCCCGGCGTGAGCGTGTTTACCGAAAATTGCCGctct                                                                                                 <  7:149346/139‑1 (MQ=255)
                    gCCGATATTAGCTGTTGTTTCTTCATCGATACGGCCCAATGGCATTTTTGAGATGGCTTTGCTAGCGATTGTAATCGCTGAAATGCCTTTTTCAGGTTCAACCCCGGCGTGAGCGGGTTTAACGAAAATTTCCGctcg                                                                                                 >  4:204649/1‑137 (MQ=255)
                    gCCGATATTAGCTGTTGTTTCTTCATCGATACGGCCCAATGGCATTTTTGAGATGGCCTTGCTAGCGATTGTAATCGCTGAAATGCCTTTTTCAGGTTCAACCCCGGCGTGGGCGGGTTTGGCGAAAATTTACGCTCtt                                                                                                >  2:226125/1‑139 (MQ=255)
                    gCCGATATTAGCTGTTGTTTCTTAATCGATACGGCCCAATGGCATTTTTGAGAAGGCTTTGCTAGCGATTGTTATCGCTGAAATGCCTTTTTCAGGTTCCACCCCGGCGTGCCGGGGTGTACCGAAAATTTCCGctaat                                                                                                >  2:205827/1‑136 (MQ=255)
                      cGATATTAGCTGTTGTTTCTTCATCGATACGGCCCAATGGCATTTTTGAGATGGCTTTGCTAGCGATTGTAATCGCTGAAATGCCTTTTTCAGGTTCAACCCCGGCGTGAGCGGTTTTAACGAAAATTTCCGCTCTTAc                                                                                              >  6:108120/1‑139 (MQ=255)
                             aGCTGTTGTTTCTTCATCGATACGGCCCAATGGCATTTTTGAGATGGCTTTGCTAGCGATTGTAATCGCTGAAATGCCTTTTTCAGGTTCAACCCCGGCGTGAGCGGTTTTCCCGAAAATTGCCGCTCTTACCTTCGc                                                                                        <  2:256123/138‑1 (MQ=255)
                                   tgttTCTTCATCGATACGGCCCAATGGCATTTTTGAGATGGCTTTGCTAGCGATTGTAATCGCTGAAATGCCTTTTTCAGGTTCAACCCCGGCGTGAGCGGTTTTGCCGAAAATTGCCGCTCTTACCTTCGCCTgtgtg                                                                                 >  5:138240/1‑138 (MQ=255)
                                     tttCTTCATCGATACGGCCCAATGGCATTTTTGAGATGGCTTTGCTAGCGATTGTAATCGCTGAAATGCCTTTTTCAGGTTCAACCCCGGCGTGGAGGCGTTTGTCGAAAATTTCCGCGATTACCTTAGCCGGTGTggg                                                                               >  3:36951/1‑139 (MQ=255)
                                            aTCGATACGGCCCAATGGCATTTTTGAGATGGCTTTGCTAGCGATTGTAATCGCTGAAATGCCTTTTTCAGGTTCAACCCCGGCGTGAGCGGTTTTCCCGAAAATTGCCGCTCTTACCTTCGCCTGTGTCGGCGCGGc                                                                         <  3:204649/138‑1 (MQ=255)
                                               gATACGGCCCAATGGCATTTTTGAGATGGCTGTGCTAGCGATTGTAATCGCTGAAATGCCTTTTTCAGGTTCAACCCCGGCGTGAGCGGTTTTCCCGAAAATAGCCGCTCTTACCTTCGCCTGTGTCGGCGCGGCcac                                                                      <  5:185165/138‑1 (MQ=255)
                                                      cccAATGGCATTTTTGAGATGGCTTTGCTAGCGATTGTAATCGCTGAAATGCCTTTTTCAGGTTCAACCCCGGCGTGCGCGGGTTTGCAGAAAATTTGCGCGGTTACCTTAGACTGTTGGGGCGCGGCCCCAAtctatt                                                              >  6:43669/1‑134 (MQ=255)
                                                                tttttGAGATGGCTTTGCTAGCGATTGTAATCGCTGAAATGCCTTTTTCAGGTTCAACCCCGGCGTGCGGGGGTTTTCCGAAAATTTTCCGTCGTAACGGAGCCCGTGTAGGCGGGGCCCCCAAGATATTTGCgccgtt                                                    >  2:109245/1‑134 (MQ=255)
                                                                tttttGAGATGGCTTTGCTAGCGATTGTAATCGCTGAAATGCCTTTTTCAGGTTCAACCCCGGCGTGAGCGGTTTTCCCGAAAATTGCCGCTCTTACCTTCGCCTGTGTCGGCGCGGCCACAATGATATTTCCGACttt                                                    >  7:30516/1‑139 (MQ=255)
                                                                                    gCGATTGTAATCGCTGAAATGCCTTTTTCAGGTTCACCCCCGGCGTGAGCGGTTTTCCCGAAAATTGCCGCTCTTACCTTCGCCTGTGTCGGCGCGGCCACAATGATATTTCCGACTTTTCCGTCTGAATCAAGAGCa                                 <  6:171875/138‑1 (MQ=255)
                                                                                    gCGATTGTAATCGCTGAAATGCCTTTTTCAGGTTCAACCCCGGCGTGAGCGGTTTTCCCGAAAATTGCCGCTCTTACCTTCGCCTGTGTCGGCGCGGCCACAATGATATTTCCGACTTTTCCGTCTGAATCAAGAGCa                                 <  3:16528/138‑1 (MQ=255)
                                                                                    gCGATTGTAATCGCTGAAATGCCTTTTTCAGGTTCAACCCCGGCGTGAGCGGTTTTCCCGAAAATTGCCGCTCTTACCTTCGCCTGTGTCGGCGCGGCCACAATGATATTTCCGACTTTTCCGTCTGAATCAAGAGCa                                 <  5:43669/138‑1 (MQ=255)
                                                                                    gCGATTGTAATCGCTGAAATGCCTTTTTCAGGTTCAACCCCGGCGTGAGCGGTTTTCCCGAAAATTGCCGCTCTTACCTTCGCCTGTGTCGGCGCGGCCACAATGATATTTCCGACTTTTCCGTCTGAATCAAGAGCa                                 <  8:127069/138‑1 (MQ=255)
                                                                                      gATTGTAATCGCTGAAATGCCTTTTTCAGGTTCAACCCCGGCGTGAGCGGTTTTCCCGAAAATTGCCGCTCTTACCTTCGCCTGTGTCGGCGCGGCCACAATGATATTTCCGACTTTTCCGTCTGAATCAAGAGCATAg                              <  8:107945/139‑1 (MQ=255)
                                                                                        ttGTAATCGCTGAAATGCCTTTTTCAGGTTCAACCCCGGCGTGAGCGGTTTTCCCGAAAATTGCCGCTCTTACCTTCGCCTGTGTCGGCGCGGCCACAATGATATTTCCGACTTTTCCGTCTGAATCAAGAGCATAGcc                            <  8:30516/139‑1 (MQ=255)
                                                                                           tAATCGCTGAAATGCCTTTTTCAGGTTCAACCCCGGCGTGAGCGGTTTTCCCGAAAATTGCCGCTCTTACCTTCGCCTGTGTCGGCGCGGCCACAATGATATTTCCGACTTTTCCGTCTGAATCAAGAGCATAGCCGTa                         <  1:226125/139‑1 (MQ=255)
                                                                                           tAATCGCTGAAATGCCTTTTTCAGGTTCAACCCCGGCGTGAGCGGTTTTCCCGAAAATTGCCGCTCTTACCTTCGCCTGTGTCGGCGCGGCCACAATGATATTTCCGACTTTTCCGTCTGAATCAAGAGCATAGCCGTa                         <  8:159763/139‑1 (MQ=255)
                                                                                                    aaaTGCCTTTTTCAGGTTCAACCCCGGCGTGAGCGGTTTTCCCGAAAATTGCCGCTCTTACCTTCGCCTGTGTCGGCGCGGCCACAATGATATTTCCGACTTTTCCGTCTGAATCAAGAGCATAGCCGTAGGATGCAGt                <  3:196731/139‑1 (MQ=255)
                                                                                                               tCAGGTTCAACCCCGGCGTGAGCGGGTTTCCCGAAAATTGCCGCTCTTACCTTCGCCTGTGTCGGCGCGGCCACAATGATATTTCCGACTTTTCCGTCTGAATCAAGAGCATAGCCGTAGGATGCAGTGATCATAGaa      <  6:180936/138‑1 (MQ=255)
                                                                                                                cAGGTTCAACCCCGGCGTGAGCGGTTTTCCCGAAAATTGCCGCTCTTACCTTCGCCTGTGTCGGCGCGGCCACAATGATATTTCCGACTTTTCCGTCTGAATCAAGAGCATAGCCGTAGGATGCAGTGATCATAGAAc     <  1:207286/138‑1 (MQ=255)
                                                                                                                   gTTCAACCCCGGCGTGAGCGGTTTTACCGAAAATTGGCGCACATTACTTCGGCATTGTAGGGGCGGCCACAAAGTTATTTAACACATTTACGTCAGAATAAAAATCAAAACAAAATGTTGGAGTTATCATAGAACGGt  >  8:227030/1‑138 (MQ=255)
                                                                                                                                        |                                                                                                                    
TGTGTGCCGCCTTCAAACCGGCCGATATTAGCTGTTGTTTCTTCATCGATACGGCCCAATGGCATTTTTGAGATGGCTTTGCTAGCGATTGTAATCGCTGAAATGCCTTTTTCAGGTTCAACCCCGGCGTGAGCGGTTTTCCCGAAAATTGCCGCTCTTACCTTCGCCTGTGTCGGCGCGGCCACAATGATATTTCCGACTTTTCCGTCTGAATCAAGAGCATAGCCGTAGGATGCAGTGATCATAGAACGGT  >  CP006881/2303961‑2304213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑