| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 2,896,953 | T→C | 70.7% | K72E (AAG→GAG) | U712_14940 ← | Uncharacterized protein yteU |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 2,896,953 | 0 | T | C | 70.7% | 61.0 / 28.2 | 41 | K72E (AAG→GAG) | U712_14940 | Uncharacterized protein yteU |
| Reads supporting (aligned to +/- strand): ref base T (6/6); new base C (17/12); total (23/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.53e-01 | |||||||||||
CAAATATCATAATGGCGAAGCGCAGGATGTGCAGAAGGAAATGACTTGGATAAATTAAGGCCAAATCAATATAAATGATCACTCCGATGAGCGCCAGTACAGCGCCGAGCAGATTTGAACGAAAAAATTCACCCTTGTATTCCTGCCAAAACGTTTTGAGCACCGGAACGTTGTCCTGCCCCTGGATCCACTTCCGCATGACAGCGAATAAAGCCGCGGTCGCGGGCATGATGCCAAACACGCCGAGACCGAGCAACGTA > CP006881/2896818‑2897077 | cAAATATCATAATGGCGAAGCGCAGGATGTGCAGAAGGAAATGACTTGGATAAATTAAGGCCAAATCAATATAAATGATCACTCCGATGAGCGCCAGTACAGCGCCGAGCAGATTTGAACGAAAAAATTCACCCTTGTa < 7:145871/139‑1 (MQ=255) aTGTGCAGAAGTAAATGACTTGGATAAATTAAGGCCAAATCAATATAAATCATCACTACGATGAGCGCCAGTACAGCGCCGAGCAGATTTGAAAGAAAAAATTCACCCTCGTATTCCTGCCAAAACGTTTTGAGCACCg < 3:158987/139‑1 (MQ=255) tgCAGAAGGAAATGACTTGGATAAATTAAGGCCAAATCAATATAAATGATCACTCCGATGAGCGCCAGTACAGCGCCGAGCAGATTTGAACGAAAAAATTCACCCTCGTATTCCTGCCAAAACGTTTTGAGCACCGGaa > 3:47365/1‑139 (MQ=255) gCAGAAGGAAATGACTTGGATAAATTAAGGCCAAATCAATATAAATGATCACTCCGATGAGCGCCAGTACAGCGCCGAGCAGATTTGAACGAAAAAATTCACCCTCGTATTCCTGCCAAAACGTTTTGAGCACCTGAAc > 1:234355/1‑139 (MQ=255) gCAGAAGGAAATGACTTGGATAAATTAAGGCCAAATCAATATAAATGATCACTCCGATGAGCGCCAGTACAGCGCCGAGCAGATTTGAACGAAAAAATTCACCCTCGTATTCCTGCCAAAACGTTTTGAGCACCGGAAc > 5:244608/1‑139 (MQ=255) cAGAAGGAAATGACTTGGATAAATTAAGGCCAAATCAATATAAATGATCACTCCGATGAGCGCCAGTACAGCGCCGAGCAGATTTGAACGAAAAAATTCACCCTTGTATTCCTGCCAAAACGTTTTGAGCACCGGAACg < 5:39990/139‑1 (MQ=255) cgAAATGACTTGGATAAATTAAGGCCAAATCAATATAAATGATCACTCCGATGAGCGCCAGTACAGCGCCGAGCAGATTTGAACGAAAAAATTCACCCTCGTATTCCTGCCAAAACGTTTTGAGCACCGGAACGTTGTc < 6:273969/138‑1 (MQ=255) aaTGACTTGGATAAATTAAGGCCAAATCAATATAAATGATCACTCCGATGAGCGCCAGTACAGCGCCGAGCAGATTTGAACGAAAAAATTCACCCTTGTATTCCTGCCAAAACGTTTTGAGCACCGGAACGTTGTCCTg > 5:281601/1‑139 (MQ=255) gATAAATTAAGGCCAAATCAATATAAATGATCACTCCGATGAGCGCCAGTACAGCGCCGAGCAGATTTGAACGAAAAAATTCACCCTTGTATTCCTGCCAAAACGTTTTGAGCACCGGAACGTTGTCCTGCCCCt > 7:106107/1‑135 (MQ=255) gATAAATTAAGGCCAAATCAATATAAATGATCACTCCGATGAGCGCCAGTACAGCGCCGAGCAGATTTGAACGAAAAAATTCACCCTTGTATTCCTGCCAAAACGTTTTGAGCACCGGAACGTTGTCCTGCCCCt < 8:106107/135‑1 (MQ=255) aTCAATATAAATGATCACTCCGATGAGCGCCAGTACAGCGCCGAGCAGATTTGAACGAAAAAATTCACCCTTGTATTCCTGCCAAAACGTTTTGAGCACCGGAACGTTGTCCTGCCCCTGGATCCACTTCCGCATGACa < 4:184296/139‑1 (MQ=255) aTCAATATAAATGATCACTCCGATGAGCGCCAGTACAGCGCCGAGCAGATTTGAACGAAAAAATTCACCCTCGTATTCCTGCCAAAACGTTTTGAGCACCGGAACGTTGTCCTGCCCCTGGATCCACTTCCGCATGACa < 3:103825/139‑1 (MQ=255) aTCAATATAAAAGATCACTCCGATGAGCGCCAGTACAGCGCCGAGCAGATTTGAACGAAAAAATTCACCCTCGTATTCCTGCCAAAACGTTTTGAGCACCGGAACGTTGTCCTGCCCCTGGATCCACTTCCGCATGACa < 8:200960/139‑1 (MQ=255) aTCACTCCGATGAGCGCCAGTACAGCGCCGAGCAGATTTGAACGAAAAAATTCCCCCTCGTATTCCTGCCAAAACGTTTTGAGCACCGGAACGTTGTCCTGCCCCTGGATCCACTGCCGCATGACAGCGAATAAAGCcg > 6:29516/1‑139 (MQ=255) aTCACTCCGATGAGCGCCAGTACAGCGCCGAGCAGATTTGAACGAAAAAATTCACCCTTGTATTCCTGCCAAAACGTTTTGAGCACCGGAACGTTGTCCTGCCCCTGGATCCACTTCCGCATGACAGCGAATAAAGCcg < 3:11545/139‑1 (MQ=255) aTCACTCCGATGAGCGCCAGTACAGCGCCGAGCAGATTTGAACGAAAAAATTCACCCTCGTATTCCTGCCAAAACGTTTTGAGCACCGGAACGTTGTCCTGCCCCTGGATCCACTTCCGCATGACAGCGAATAAAGCcg > 7:142769/1‑139 (MQ=255) cACTCCGATGAGCGCCAGTACAGCGCCGAGCAGATTTGAACGAAAAAATTCACCCTCGTATTCCTGCCAAAACGTTTTGAGCACCg < 6:150532/86‑1 (MQ=255) cACTCCGATGAGCGCCAGTACAGCGCCGAGCAGATTTGAACGAAAAAATTCACCCTCGTATTCCTGCCAAAACGTTTTGAGCACCg > 5:150532/1‑86 (MQ=255) cACTCCGATGAGCGCCAGTACAGCGCCGAGCAGATTTGAACGAAAAAATTCACCCTCGTATTCCTGCCAAAACGTTTTGAGCACCGGAACGTTGTCCTGCCCCTGGATCCACTTCCGCATGACAGCGAATAAAGCcgcg > 1:183207/1‑139 (MQ=255) cACTCCGATGAGCGCCAGTACAGCGCCGAGCAGATTTGAACGAAAAAATTCACCCTCGTATTCCTGCCAAAACGTTTTGAGCACCGGAACGTTGTCCTGCCCCTGGATCCACTTCCGCATGACAGCGAATAAAGCcgcg > 5:245666/1‑139 (MQ=255) tCCGATGAGCGCCAGTACAGCGCCGAGCAGATTTGAACGAAAAAATTCACCCTCGTATTCCTGCCAAAACGTTTTGAGCACCGGAACGTTGTCCTGCCCCTGGATCCACTTCCGCATGACAGCGAATAAAGCCGCGGTc > 1:116215/1‑139 (MQ=255) gATGAGCGCCAGTACAGCGCCGAGCAGATTTGAACGAAAAAATTCACCCTTGTATTCCTGCCAAAACGTTTTGAGCACCGGAACGTTGTCCTGCCCCTGGATCCACTTCCGCATGACAGCGAATAAAGCCGCGGTcgcg < 6:281601/139‑1 (MQ=255) gcgcCAGTACAGCGCCGAGCAGATTTGAACGAAAAACTTCACCCTCGTATTCCTGCCAAAACGTTTTGAGCACCGGAACGTTGTCCTGCCCCTGGATCCACTTCCGCATGACAGCGAATAAAGCCGCGGTCGCGGGCat < 4:26430/139‑1 (MQ=255) gcgcCAGTACAGCGCCGAGCAGATTTGAACGAAAAAATTCACCCTCGTATTCCTGCCAAAACGTTTTGAGCACCGGAACGTTGTCCTGCCCCTGGATCCACTTCCGCATGACAGCGAATAAAGCCGCGGTCGCGGGCat < 2:165149/139‑1 (MQ=255) gcgcCAGTACAGCGCCGAGCAGATTTGAACGAAAAAATTCACCCTCGTATTCCTGCCAAAACGTTTTGAGCACCGGAACGTTGTCCTGCCCCTGGATCCACTTCCGCATGACAGCGAATAAAGCCGCGGGCGCGGGCat < 6:201265/139‑1 (MQ=255) gTACAGCGCCGAGCAGATTTGAACGAAAAAATTCACCCTTGTATTCCTGCCAAAACGTTTTGAGCACCGGAACGTTGTCCTGCCCCTGGATCCACTTCCGCATGACAGCGAATAAAGCCGCGGTCGCGGGCATGATGc > 2:198397/1‑138 (MQ=255) cAGCGCCGAGCTTATTTGAACTAAAAAATGCACCCTCTTTTTGCTGCCAAACGGTTTTGAGCACCGGAACGTTGTCCTGCCCCTGGTTCCACTTCCGCATGACAGCTAATAAACCCGCGGGCGGGGGAATGTTGCCaaa < 6:244608/139‑1 (MQ=255) cgcCGAGCAGATTTGAACGAAAAAATTCACCCTCGTATTCCTGCCAAAACGTTTTGAGCACCGGAACGTTGTc > 6:7137/1‑73 (MQ=255) cgcCGAGCAGATTTGAACGAAAAAATTCACCCTCGTATTCCTGCCAAAACGTTTTGAGCACCGGAACGTTGTc < 5:7137/73‑1 (MQ=255) cgcCGAGCAGATTTGAACGAAAAAATTCACCCTCGTATTCCTGCCAAAACGTTTTGAGCACCGGAACGTTGTCCTGCCCCt < 8:42079/81‑1 (MQ=255) cgcCGAGCAGATTTGAACGAAAAAATTCACCCTCGTATTCCTGCCAAAACGTTTTGAGCACCGGAACGTTGTCCTGCCCCt > 7:42079/1‑81 (MQ=255) cgcCGAGCAGATTTGAACGAAAAAATTCACCCTCGTATTCCTGCCAAAACGTTTTGAGCACCGGAACGTTGTCCTGCCCCTGGATCCACTTCCGCATGACAGCGAATAAAGCCGCGGTCGCGGGCa > 8:20317/1‑126 (MQ=255) cgcCGAGCAGATTTGAACGAAAAAATTCACCCTCGTATTCCTGCCAAAACGTTTTGAGCACCGGAACGTTGTCCTGCCCCTGGATCCACTTCCGCATGACAGCGAATAAAGCCGCGGTCGCGGGCa < 7:20317/126‑1 (MQ=255) cgcCGAGCAGATTTGAACGAAAAAATTCACCCTCGTATTCCTGCCAAAACGTTTTGAGCACCGGAACGTTGTCCTGCCCCTGGATCCACTTCCGCATGACAGCGAATAAAGCCGCGGTCGCGGGCATGATGCCAAacac > 8:223669/1‑139 (MQ=255) cgcCGAGCAGATTTGAACGAAAAAATTCACCCTCGTATTCCTGCCAAAACGTTTTGAGCACCGGAACGTTGTCCTGCCCCTGGATCCACTTCCGCATGACAGCGAATAAAGCCGCGGTCGCGGGCATGATGCCAAacac > 2:61307/1‑139 (MQ=255) ccGAGCAGATTTGAACGAAAAAATTCACCCTTGTATTCCTGCCAAAACGTTTTGAGCACCGGAACGTTGTCCTGCCCCTGGATCCACTTCCGCATGACATCGAATAAAGCCGCGGTCTCGGGCATGATGCCAAACACGc > 5:138458/1‑139 (MQ=255) aGCAGATTTGAACGAAAAAATTCACCCTTGTATTCCTGCCAAAACGTTTTGAGCACCGGAACGTTGTCCTGCCCCTGGATCCACTTCCGCATGACAGCGAATAAAGCCGCGGTCGCGGGCATGATGCCAAACACGcc > 2:905/1‑137 (MQ=255) aGCAGATTTGAACGAAAAAATTCACCCTCGTGTTCCTGCCAAAACGTTTTGAGCACCGGAACGTTGTCCTGCCCCTGGATCCACTTCCGCATGACAGCGAATAAAGCCGCGGTCGCGGGCATGCTGCCAAACACGcc > 4:179373/1‑137 (MQ=255) aGCAGATTTGAACGAAAAAATTCACCCTCGTATTCCTGCCAAAACGTTTTGAGCACCGGAACGTTGTCCTGCCCCTGGATCCACTTCCGCATGACAGCGAATAAAGCCGCGGTCGCGGGCATGATGCCAAACACGcc > 6:252610/1‑137 (MQ=255) aaCGAAAAAATTCACCCTTGTATTCCTGCCAAAACGTTTTGAGCACCGGAACGTTGTCCTGCCCCTGGATCCACTTCCGCATGACAGCGAATAAAGCTGCGGTCGCGGGCATGATGCCAAACACGCCGAGACCGAGCaa > 3:186408/1‑139 (MQ=255) aaaaaTTCACCCTCGTATTCCTGCCAAAACGTTTTGAGCACCGGAACGTTGTCCTGCCCCTGGATCCACTTCCGCATGACAGCGAATAAAGCCGCGGTCGCGGGCATGATGCCAAACACGCCGAGACCGAGCAACGTa > 7:225100/1‑138 (MQ=255) | CAAATATCATAATGGCGAAGCGCAGGATGTGCAGAAGGAAATGACTTGGATAAATTAAGGCCAAATCAATATAAATGATCACTCCGATGAGCGCCAGTACAGCGCCGAGCAGATTTGAACGAAAAAATTCACCCTTGTATTCCTGCCAAAACGTTTTGAGCACCGGAACGTTGTCCTGCCCCTGGATCCACTTCCGCATGACAGCGAATAAAGCCGCGGTCGCGGGCATGATGCCAAACACGCCGAGACCGAGCAACGTA > CP006881/2896818‑2897077 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |