Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 2,963,941 A→G 100% intergenic (‑128/+152) U712_15285 ← / ← U712_15290 Manganese‑binding lipoprotein mntA/o‑succinylbenzoate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,963,9410AG79.9% 80.4 / 15.7 41intergenic (‑128/+152)U712_15285/U712_15290Manganese‑binding lipoprotein mntA/o‑succinylbenzoate synthase
Reads supporting (aligned to +/- strand):  ref base A (7/1);  new base G (25/6);  total (32/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.66e-01
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTCTCATATTTCTCCTCCTCTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCGCGTTTGGCCATTTTT‑GTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCATT  >  CP006881/2963807‑2964065
                                                                                                                                      |                                                                                                                             
gTCTCATATTTCTCCTCCTCTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAGATTa                                                                                                                           >  2:26326/1‑139 (MQ=255)
     atatTTCTCCTCCTCTTTGCCATCAATGTCCGAATCTTTAATCAAATGTTGTCACTACATAGACATGAGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTATCCCTCATGCAAGATTATctag                                                                                                                      >  7:63482/1‑136 (MQ=255)
           ctcctcctcTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAGATTATCGCGTTTgg                                                                                                                 <  8:63482/138‑1 (MQ=255)
           ctcctcctcTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAGATTATCGCGTTTGGc                                                                                                                >  8:241548/1‑139 (MQ=255)
           ctcctcctcTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGc                                                                                                                >  8:47027/1‑139 (MQ=255)
           ctcctactcTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAGATTATCGCGTTTGGc                                                                                                                >  1:103046/1‑139 (MQ=255)
                 ctctTTGCCATCAATGTACGATTGTGTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGATTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAGATTATCGCGTGTGGCCAtt                                                                                                            <  1:107791/137‑1 (MQ=255)
                   ctTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAGATTATCGCGTTTGGCCATTTTT‑g                                                                                                       >  7:197495/1‑139 (MQ=255)
                       gCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAGATTATCGCGTTTGGCCATTTTT‑GTCa                                                                                                    <  6:134789/138‑1 (MQ=255)
                           tCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAGATTATCGCGTTTGGCCATTTTT‑GTCAAGCt                                                                                                >  6:116224/1‑138 (MQ=255)
                              aTGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAGATTATCGCGTTTGGCCATTTTT‑GTCAAGCTGAtt                                                                                            >  8:71804/1‑139 (MQ=255)
                              aTGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTT‑GTCAAGCTGAtt                                                                                            >  3:11683/1‑139 (MQ=255)
                                         ttttATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAGATTATCGCGTTTGGCCATTTTT‑GTCAAGCTGATTTACATaaaaa                                                                                  >  5:265305/1‑138 (MQ=255)
                                           ttATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAGATTATCGCGTTTGGCCATTTTT‑GTCAAGCTGATTTACATAAAAAAAc                                                                               >  5:264764/1‑139 (MQ=255)
                                                        tCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAGATTATCGCGGTTGGCCATTTTT‑GTCAAGCTGATTTACATAAAAAAACCGGCTGGGTcagc                                                                  >  4:57306/1‑139 (MQ=255)
                                                                 aGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAGATTATCGCGTTTGGCCATTTTT‑GTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTc                                                          >  7:99414/1‑138 (MQ=255)
                                                                      tgtgACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAGATTATCGCGTTTGGCCATTTTT‑GTCAAGCTGATTTACATAAAAAATCCGGCTGGGTCAGCTGCCGGTCCTAAc                                                     >  2:133120/1‑138 (MQ=255)
                                                                      tgtgACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAGATTATCGCGTTTGGCCATTTTT‑GTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAAc                                                     >  3:37837/1‑138 (MQ=255)
                                                                      tgtgACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAGATTATCGCGTTTGGCCATTTTT‑GTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAAc                                                     >  4:199339/1‑138 (MQ=255)
                                                                          aaaTTAAATAACCCAAACGCTTGTTTTATTTCCACAGCAAAAAACGTTACCCTCATGAAAGATTATCGCGTTGGCCCATTTTT‑GACACGCTGATTAAAAAAAAAAAACCGGCGGGGTCAGCAGCCGGTCCAAACGAGaa                                                <  6:264764/137‑1 (MQ=255)
                                                                               aaaTTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAGATTATCGCGTTTGGCCATTTTT‑GTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGAt                                           >  2:44258/1‑139 (MQ=255)
                                                                                         aaaGCATGTTTTATTAACACAGCAAAAAAAGTTTCCCTCATGCAATATTATCGCGTTTGGCAATTTTT‑GTCATGCTTATTAACATAAAAAACCCGGCTGGGGCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACg                                 <  7:241548/139‑1 (MQ=255)
                                                                                          aaGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTT‑GTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACg                                 >  3:234093/1‑138 (MQ=255)
                                                                                               tGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAGATTATCGCGTTTGGCCATTTTT‑GTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACt                           >  7:82970/1‑139 (MQ=255)
                                                                                               tGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAGATTATCGCGTTTGGCCATTTTT‑GTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACt                           >  3:252876/1‑139 (MQ=255)
                                                                                                 ttttATTTACACAGGACAAAAAGTTTCCCTCATGCAAGATTATCGCGTGTGGCCATTTTT‑GTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACtgt                         >  1:125441/1‑137 (MQ=255)
                                                                                                  ttattttaCACAGGAAAAAACGTTTCCCTCATCCAAGATTATGGTGTTGGGCATTTTTT‑TTAACGCTTATTAAAAAAAAAAAACGGGCGGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACttt                         <  8:99414/134‑1 (MQ=255)
                                                                                                     atttaCACACTAAAAAAATTTCCCCTCATCCAAGATAATCGCGTTGGGACATTTTT‑GGCACGCTGATTAAAAAAAAAACCCCGGCGGGGCCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTAc                      <  4:210086/138‑1 (MQ=255)
                                                                                                     attaaCCCAGTACAAAAAGTTTCCCTCTTGAATTATTATGGCGTTTGGCATTTTTT‑GACAAGCTTATTTAAATAAAAAACCCGGCTGGGACAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTAc                      <  5:116224/138‑1 (MQ=255)
                                                                                                         aaacaGCACAAAACGTTACCCACATCCAAAATTA‑CCCGGTTGGCCATTTTTGGCCAAGCTGATTAAAAAAAAAAACCCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTaa                 <  8:78490/137‑1 (MQ=255)
                                                                                                           acaGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTT‑GTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTaa                 >  5:118732/1‑137 (MQ=255)
                                                                                                                aCAAAAAGTTTCCCTCATGCAAGATTATCGCGTTTGGCCATTTTT‑GTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGca            >  1:54365/1‑137 (MQ=255)
                                                                                                                         ttCCCTCATGCAAGATTATCGCGTTTGGCCATTTTT‑GTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCAtt  >  6:268891/1‑138 (MQ=255)
                                                                                                                         ttCCCTCATGCAAGATTATCGCGTTTGGCCATTTTT‑GTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCAtt  >  7:106752/1‑138 (MQ=255)
                                                                                                                         ttCCCTCATGCAAGATTATCGCGTTTGGCCATTTTT‑GTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCAtt  >  5:252344/1‑138 (MQ=255)
                                                                                                                         ttCCCTCATGCAAGATTATCGCGTTTGGCCATTTTT‑GTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCAtt  >  3:29843/1‑138 (MQ=255)
                                                                                                                         ttCCCTCATGCAAGATTATCGCGTTTGGCCATTTTT‑GTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCAtt  >  3:259405/1‑138 (MQ=255)
                                                                                                                         ttCCCTCATGCAAAATTATCGCGTTTGGCCATTTTT‑GTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCAtt  >  1:49724/1‑138 (MQ=255)
                                                                                                                         ttCCCTCATGCAAAATTATCACGTTTGGCCATTTTT‑GTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCAtt  >  5:270514/1‑138 (MQ=255)
                                                                                                                         ttCCCTCATGCAAAATTATCACGTTTGGCCATTTTT‑GTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCAtt  >  2:273554/1‑138 (MQ=255)
                                                                                                                         ttCCCTAATGCAAGATTATCGCGTTTGGCCATTTTT‑GTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTCTAACAGCACAGTGTCAtt  >  7:92650/1‑138 (MQ=255)
                                                                                                                                      |                                                                                                                             
GTCTCATATTTCTCCTCCTCTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCGCGTTTGGCCATTTTT‑GTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCATT  >  CP006881/2963807‑2964065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑