Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,112,607 A→G 5.5% I162V (ATT→GTT)  U712_15960 → Putative biotin transporter BioYB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,112,6070AG 5.5% 230.2 / 3.5 73I162V (ATT→GTT) U712_15960Putative biotin transporter BioYB
Reads supporting (aligned to +/- strand):  ref base A (40/29);  new base G (2/2);  total (42/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

CAAGTAAAATTCGATTTCTCATTGCTTCACTTATCGGAACGGCGATCATGTATCTCATCGGCACTACCTATATGTATCTTGCACTGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCAGCAATCTACCGCTCTATTCATAAA‑GCAACCGGCTTTAACAGAAATCACATATCATCTACGTAAAAACCGTTCTATTGG  >  CP006881/3112470‑3112741
                                                                                                                                         |                                                                                                                                       
ctaGTAAAATTCGATTTCTCATTGCTTCACTTATCGGAACGGCGATCATGTATCTCATCGGCACTACCTATATGTATCTTGCACTGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGAt                                                                                                                                        <  3:74367/137‑1 (MQ=255)
 aaGTAAAATTCGATTTCTCATTGCTTCACTTATCGGAACGGCGATCATGTATCTCATCGGCACTACCTATATGTATCTTGCACTGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGAtt                                                                                                                                       <  8:82350/139‑1 (MQ=255)
      aaaTTCGATTTCTCATTGCTTCACTTATCGGAACGGCGATCATGTATCTCATCGGCACTACCTATATGTATCTTGCACTGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGtt                                                                                                                                  <  8:154386/139‑1 (MQ=255)
                             cTTATCGGAACGGCGATCATGTATCTCATCGGCACTACCTATATGTATCTTGCACTGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTCTGATTTGGTTTATGGTAAAAGATACAGCgctgg                                                                                                           <  6:264029/139‑1 (MQ=255)
                                                     ctcATCGGCACTACCTATATGTATCTTGCACTGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCGTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCAc                                                                                   <  5:67362/139‑1 (MQ=255)
                                                        aTCGGCACTCACTATATGTATCTTGCACTGAAGCAGTGGATTCATACGCCAGTGAGCTATGGCACGCCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGAGCTGGCTGTGATACTGTCTTTTATGGCACCAg                                                                                >  8:159235/1‑139 (MQ=255)
                                                        aTCGGCACTACCTATATGTATCTTGCACTGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCAc                                                                                   <  5:220212/136‑1 (MQ=255)
                                                          cGGCACTACCTATATGTATCTTGCACTGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCAGc                                                                               >  5:226968/1‑138 (MQ=255)
                                                           ggCACTACCTATATGTATCTTGCACTGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCAGCa                                                                              <  5:286577/138‑1 (MQ=255)
                                                             cACTACCTATATGTATCTTGCACTGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCAGCAATc                                                                           <  6:226968/139‑1 (MQ=255)
                                                               cTACCTATATGTATCTTGCACTGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCAGCAATCTa                                                                         <  4:235599/139‑1 (MQ=255)
                                                                  ccTATATGTATCTTGCACTGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAgcg                                                                                                               >  6:270278/1‑98 (MQ=255)
                                                                  ccTATATGTATCTTGCACTGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAgcg                                                                                                               <  5:270278/98‑1 (MQ=255)
                                                                     atatGTATCTTGCACTGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATAGCACCAGCAATCTACCGcgc                                                                   >  3:278667/1‑137 (MQ=255)
                                                                       atGTATCTTGCACTGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCAGCAATCTACCGCTCTa                                                                 >  6:237870/1‑139 (MQ=255)
                                                                        tGTATCTTGCACTGAAGCTGGGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCAGCAATCTACCGCTCTa                                                                 <  4:126620/138‑1 (MQ=255)
                                                                            tCTTGCACTGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGGTTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCAGCAATCTACCGCTCAATTCa                                                             >  8:205818/1‑138 (MQ=255)
                                                                            tCTTGCACTGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGGTTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTGTATCGCACCAGCAATCTACCGCTCAATTCa                                                             >  8:201967/1‑138 (MQ=255)
                                                                            tCTTGCACTGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCgctggctg                                                                                                        >  4:224866/1‑95 (MQ=255)
                                                                            tCTTGCACTGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCgctggctg                                                                                                        <  3:224866/95‑1 (MQ=255)
                                                                            tCTTGCACTGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCgct                                                                                                             <  1:179876/90‑1 (MQ=255)
                                                                            tCTTGCACTGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCgct                                                                                                             >  2:179876/1‑90 (MQ=255)
                                                                            tCTTGCACTGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCCCCAGCCATCTACCGCTCTATTCa                                                             >  6:93691/1‑138 (MQ=255)
                                                                            tCTTGCACTGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCCGCAATCTACCGCTCTATTCa                                                             >  5:124302/1‑138 (MQ=255)
                                                                            tCTTGCACTGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCAGCAATCTACCg                                                                      <  7:169571/129‑1 (MQ=255)
                                                                            tCTTGCACTGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCAGCAATCTACCg                                                                      >  8:169571/1‑129 (MQ=255)
                                                                            tCTTGCACTGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCAGCAATCTACCGctct                                                                  >  5:231170/1‑133 (MQ=255)
                                                                            tCTTGCACTGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCAGCAATCTACCGctct                                                                  <  6:231170/133‑1 (MQ=255)
                                                                            tCTTGCACTGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCAGCAATCTACCGctct                                                                  <  4:130450/133‑1 (MQ=255)
                                                                            tCTTGCACTGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCAGCAATCTACCGctct                                                                  >  3:130450/1‑133 (MQ=255)
                                                                            tCTTGCACTGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCAGCAATCTACCGCTCTATTCa                                                             >  4:283774/1‑138 (MQ=255)
                                                                            tCTTGCACTGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCAGCAATCTACCGCTCTATTCa                                                             >  1:183319/1‑138 (MQ=255)
                                                                            tCTTGCACTGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCAGCAATCTACCGCTCTATTCa                                                             >  4:8400/1‑138 (MQ=255)
                                                                            tCTTGCACTGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCAGCAATCTACCGCTCTATTCa                                                             >  2:60468/1‑138 (MQ=255)
                                                                            tCTTGCACTGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTGTATAGCACCAGCAATCTACCGCGCTAttga                                                             >  1:145029/1‑136 (MQ=255)
                                                                             cTTGCACTGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTGTTCGCACCAGCAATGTACCGCTGTaatgat                                                            >  1:104553/1‑133 (MQ=255)
                                                                              ttGCACTGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCAGCAATCTACCGCTCTATTCATa                                                           >  8:185744/1‑138 (MQ=255)
                                                                              ttGCACTGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTGTATCGCCCCAGCAATCTACCGCGCTATTCaaa                                                           >  8:189963/1‑136 (MQ=255)
                                                                                gCACTGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTGTATCGCACCAGCCATCTACCGCTCTATTCATAAA‑g                                                       >  6:221555/1‑139 (MQ=255)
                                                                                gCACTGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTAGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGAGCTGGCTGTGATACTGTCGTTTATCGCACCAGCAATCAACCGCTATATTaaaaaa                                                         >  7:2154/1‑138 (MQ=255)
                                                                                 cACTGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTAACGCACCAGCAATCTACCGCTCTATTAAAAAA‑g                                                       >  8:2756/1‑138 (MQ=255)
                                                                                    tGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTa                                                                                                                                >  4:97395/1‑63 (MQ=255)
                                                                                    tGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTa                                                                                                                                <  3:97395/63‑1 (MQ=255)
                                                                                    tGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCAGCAATCTACCGCTCTATTCATAAA‑GCAAc                                                   >  2:248792/1‑139 (MQ=255)
                                                                                      aaGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCCCCAGCCATCTACCGCTCTATTAATAAA‑GCaaacc                                                 >  5:222183/1‑136 (MQ=255)
                                                                                      aaGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCAGCaa                                                                             <  5:187540/112‑1 (MQ=255)
                                                                                      aaGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCAGCaa                                                                             >  6:187540/1‑112 (MQ=255)
                                                                                           gtgGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCgct                                                                                                             >  6:226802/1‑75 (MQ=255)
                                                                                           gtgGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCgct                                                                                                             <  5:226802/75‑1 (MQ=255)
                                                                                            tgGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCAGCAATCTACCGATCTATTAATAAA‑GCAACCGGCttt                                            >  8:259304/1‑138 (MQ=255)
                                                                                              gATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTGTATCGCACCAGCAATCGACCGCTCAATTCATAAA‑TCAACCCGCGTTaa                                          >  4:56142/1‑138 (MQ=255)
                                                                                                ttCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCCGCAATCGACCGCGCTATTCATAAA‑CCAACAGGCtttta                                          >  7:180563/1‑134 (MQ=255)
                                                                                                ttCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCAGCAATCTACCGCTCTATTCATAAA‑GCAACCGGCtttta                                          >  1:240493/1‑134 (MQ=255)
                                                                                                    tACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCAGCAATCTACGGCTCTATTCATAAA‑GCAACCGGCTTTAACAGTAAt                                   <  3:56142/139‑1 (MQ=255)
                                                                                                    tACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCAGCAATCTACCGCTCTATTCATAAA‑GCAACCGGCTTTAACAGAAAt                                   <  1:60468/139‑1 (MQ=255)
                                                                                                    tACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCAGCAATCTACCGCTCTATTCATAAA‑GCAACCGGCTTTAACAGAAAt                                   <  7:185744/139‑1 (MQ=255)
                                                                                                    tACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCAGCAATCTACCGCTCTATTCATAAA‑GCAACCGGCTTTAACAGAAAt                                   <  7:189963/139‑1 (MQ=255)
                                                                                                    tACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCAGCAATCTACCCCTCAATTCATAAA‑GCAACCGGCTTTAAAAGAAAt                                   >  4:178722/1‑139 (MQ=255)
                                                                                                      cGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGGTTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCAGCAATCTACCGCTCAATTCATAAA‑GCAACCGGCTTTAACAGAAATca                                 <  7:205818/139‑1 (MQ=255)
                                                                                                      cGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGGTTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCAGCAATCTACCGCTCAATTCATAAA‑GCAACCGGCTTTAACAGAAATca                                 <  7:201967/139‑1 (MQ=255)
                                                                                                            tGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCAGCAATCTACCGCTCTATTCATAAA‑GCAACCGGCTTTAACAGAAATCACatat                            >  4:116312/1‑138 (MQ=255)
                                                                                                               agaccggcacggcTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCAGCAATCTACCGCTCTATTCATAAA‑GCAACCGGCTTTAACAGAAATCACATatcatc                        <  1:127467/135‑1 (MQ=255)
                                                                                                                       acggcTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCAGCAATCTACCGCTCTATTCATAAA‑GCAACCGGCTTTAACAGAAATCACATATCATCTACGTaaa                <  3:143451/139‑1 (MQ=255)
                                                                                                                        cggcTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCAGCAATCTACCGCTCTATTCATAAA‑GCAACCGGCTTTAACAGAAATCACATATCATCAAAGTaaa                >  8:166640/1‑138 (MQ=255)
                                                                                                                         ggcTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCAGCAATCTACCGCTCTATTCATAAA‑GCAACCGGCTTTAACAGAAATCACATATCATCTACGTaaaaa              <  2:183319/139‑1 (MQ=255)
                                                                                                                         ggcTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCAGCAATCTACCGCTCTATTCATAAA‑GCAACCGGCTTTAACAGAAATCACATATCATCTACGTaaaaa              <  5:237870/139‑1 (MQ=255)
                                                                                                                         ggcTTGGGGATTTATGATTTGGTTTATGGTATAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCAGCAATCTACCGCTCTATTCATATA‑GCTACCGGCTTTAACAGAAATCACATATCATCTACGTaaaaa              <  8:2154/139‑1 (MQ=255)
                                                                                                                               gggCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCAGCAATCTACCGCTCTATTCATAAA‑GCAACCGGCTTTAACAGAAATCACATATCATCTACGTAAAAACCGtt         <  1:256042/138‑1 (MQ=255)
                                                                                                                                ggCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTTTCGCACCAGCAATCTACCGCTCTATTAATAAA‑TCAACCGGCTTTAACAAAAATCAAATATAATATAAGTAAAAAcaagta        >  2:127467/1‑133 (MQ=255)
                                                                                                                                     tATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCAACAGCAAAGTACCGCTCTATTCATAAA‑TCAACCGGCGTTAACACAAATCACATATCATCTAACTAAAAACCGTTCTAtttg  >  6:286884/1‑137 (MQ=255)
                                                                                                                                     tATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATAGCACCAGCAATCTACCGCGCTATTAATAAA‑GCAACCGGCTTTAACAGAAATCACAAATCATATACGTAAAAACCCTTCTAtttg  >  2:67613/1‑137 (MQ=255)
                                                                                                                                     tATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATACCACCAGCAAAAAAACCCGCTAATCATAAATAAAACCCGC‑TTAACAAAAAACAAAAATCAAGAAAGTAAAAACCCGTCTATTgg  >  2:104421/1‑139 (MQ=255)
                                                                                                                                     tATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTGTATCGCCCCAGCCATCGACCGCGGTTTTGATAAA‑ACAACCGGCGTTTAAAAAAAACACATATCAACTACGTAAAAACAGTTCTATAgg  >  2:102233/1‑139 (MQ=255)
                                                                                                                                         |                                                                                                                                       
CAAGTAAAATTCGATTTCTCATTGCTTCACTTATCGGAACGGCGATCATGTATCTCATCGGCACTACCTATATGTATCTTGCACTGAAGCTGTGGATTCATACGCCAGTGAGCTACGGCACGGCTTGGGGCTTTATGATTTGGTTTATGGTAAAAGATACAGCGCTGGCTGTGATACTGTCGTTTATCGCACCAGCAATCTACCGCTCTATTCATAAA‑GCAACCGGCTTTAACAGAAATCACATATCATCTACGTAAAAACCGTTCTATTGG  >  CP006881/3112470‑3112741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑