| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 3,204,165 | T→G | 24.8% | V196V (GTT→GTG) | U712_16490 → | Transcriptional regulatory protein CssR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 3,204,165 | 0 | T | G | 24.8% | 62.3 / 5.6 | 42 | V196V (GTT→GTG) | U712_16490 | Transcriptional regulatory protein CssR |
| Reads supporting (aligned to +/- strand): ref base T (9/22); new base G (9/2); total (18/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.97e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATATCATCAATTTGACGTCAAAGGAATTTGATCTGCTGCTATTATTTATCCATCATAAAGGGCATCCATACTCTCGTGAGGATATCCTCCTAAAAGTGTGGGGACATGACTACTTCGGAACAGACCGGGTCGTTGATGATCTCGTCCGGAGACTGCGCAGAAAGATGCCTGAATTGAAGGTGGAGACGATTTACGGTTTCGGCTACAGGATGATGTCATCATGAAAAACAAGCCGCTCGCGTTTCAGATATGGGTTGTCATATCCGGCATCC > CP006881/3204031‑3204303 | aaTATCATCAATTTGACGTCAAAGGAATTTGATCTGCTGCTATTATTTATCCATCATAAAGGGCATCCATACTCTCGTGAGGATATCCTCCTAAAAGTGTGGGGACATGACTACTTCGGAACAGACCGGGTCGTtgatg < 2:184746/139‑1 (MQ=255) tatCATCAATTTGACGTCAAAGGAATTTGATCTGCTGCTATTATTTATCCATCATAAAGGGCATCCATACTCTCGTGAGGATATCCTCCTAAAAGTGTGGGGACATGACTACTTCGGAACAGACCGGGTCGTTgatgat < 3:265031/139‑1 (MQ=255) tatCATCAATTTGACGTCAAAGGAATTTGATCTGCTGCTATTATTTATCCATCATAAAGGGCATCCATACTCTCGGGAGGATATCCTCCTAAAAGTGTGGGGACATGACTACTTCGGAACAGACCGGGTCGTTgatgat < 7:6538/139‑1 (MQ=255) tatCATCAATTTGACGTCAAAGGAATATGATCTGCTGCTATTATTTATCCATCATAAAGGGCATCCATACTCTCGTGAGGATATCCTCCTAAAAGTGTGGGGACATGACTACTTCGGAACAGACCGGGTCGTTgatgat < 2:230407/139‑1 (MQ=255) tcatcaATTTGACGTCAAAGGAATTTGATCTGCTGCTATTATTTATCCATCATAAAGGGCATCCATACTCTCGTGAGGATATCCTCCTAAAAGTGTGGGGACATGACTACTTCGGAACAGACCGGGTCGTTGATGAtct < 8:29868/139‑1 (MQ=255) tcatcaATTTGACGTCAAAGGAATTTGATCTGCTGCTATTATTTATCCATCATAAAGGGCATCCATACTCTCGTGAGGATATCCTCCTAAAAGTGTGGGGACATGACTACTTCGGAACAGACCGGGTCGTTGATGAtct < 3:43959/139‑1 (MQ=255) tcatcaATTTGACGTCAAAGGAATTTGATCTGCTGCTATTATTTATCCATCATAAAGGGCATCCATACTCTCGTGAGGATATCCTCCTAAAAGTGTGGGGACATGACTACTTCGGAACAGACCGGGTCGTTGATGAtct < 7:208418/139‑1 (MQ=255) aCGTCAAAGGAATTTGATCTGCTGCTATTATTTATCCATCATAAAGGGCATCCATACTCTCGTGAGGATATCCTCCTAAAAGTGTGGGGACATGACTACTTCGGAACAGACCGGGTCGTTGATGATCTCGTCCGgaga < 6:135375/138‑1 (MQ=255) aCGTCAAAGGAATTTGATCTGCTGCTATTATTTATCCATCATAAAGGGCATCCATACTCTCGTGAGGATATCCTCCTAAAAGTGTGGGGACATGACTACTTCGGAACAGACCGGGTCGTTGATGATCTCGTCCGGAGAc < 7:61649/139‑1 (MQ=255) tatCCATCATAAAGGGCATCCATACTCTCGTGAGGATATCCTCCTAAAAGTGTGGGGACATGACTACTTCGGAACAGACCGGGTCGTTGATGATCTCGTCCGGAGACTGCGCAGAAAGATGCCTGAATTGAAGGTGga < 2:202094/138‑1 (MQ=255) tCCATCATAAAGGGCATCCATACTCTCGTGAGGATATCCTCCTAAAAGTGTGGGGACATGACTACTTCGGAACAGACCGGGTCGTTGATGATATCGTCCGGAGACTGCGCAGCAAGATGCCTGAATTGAAGGGGGAGAc > 7:186535/1‑139 (MQ=255) tCCATCATAAAGGGCATCCATACTCTCGTGAGGATATCCTCCTAAAAGTGTGGGGACATGACTACTTCGGAACAGACCGGGTCGTTGAAGATATAGTGCGGGGACGGCGCAGAAATATGCCAGATTTGTAGGTGgagcc > 5:183631/1‑137 (MQ=255) catAAAGGGCATCCATACTCTCGTGAGGATATCCTCCTAAAAGTGTGGGGACATGACTACTTCGGAACAGACCGGGTCGTGGTTGTTCTCGGGCGGCGCCGGCGCAGCAACATGCCGGCATTGAAGGTGGAGGCGAttt > 1:128676/1‑139 (MQ=255) aaGGGCATCCATACTCTCGTGAGGATATCCTCCTAAAAGTGTGGGGACATGACTACTTCGGAACAGACCGGGTCGTTGATGATCTCGTCCGGAGACTGCGCAGAAAGATGCCTGAATTGAAGGTGGAGACGATTTACg < 3:113561/138‑1 (MQ=255) aGGGCATCCATACTCTCGTGAGGATATCCTCCTAAAAGTGTGGGGACATGACTACTTCGGAACAGACCGGGTCGTGGATGTTGTCGTGCGGAGCCTGCGCAGCAATATGCCTGAATTGAAGGTGGAGGCGATTTACggg > 7:110580/1‑138 (MQ=255) ggCATCCATACTCTCGTGAGGATATCCTCCTAAAAGTGTGGGGACATGACTACTTCGGAACAGACCGGGTCGTTGATGATCTCGGCCGGAGACTGCGCAGAAAGATGCCTGAATTGAAGGTGGAGACGATTTACGGtt > 6:69356/1‑138 (MQ=255) ccATACGCTCGTGAGGATATCCTCCTAAACGTGTGGGGACCTGACTACTTCGGAACAGACCGGGTCGTGGATGATCTCGTCCGGAGACTGCGCAGAAAGATGCCTGAATTGAAGGGGGAGACGATTTACGGTTTCGGCt < 6:183631/139‑1 (MQ=255) gTGAGGATATCCTCCTAAAAGTGTGGGGACATGACTACTTCGGAACAGACCGGGTCGTTGATGATCTCGTCCCGAGACTGCGCTGAAATATTCCTGAATTGAAGGTGGAGACGAATTACGGGTTGGGCGACAGgttgat > 3:227890/1‑139 (MQ=255) gTGAGGATATCCTCATAAAAGTGTGGGGACATGACTAATTCGGAACAGACCGGGTCGTGGTTTATCTCGTCCGGAGACTGCGCAGAAAGATGCGTGTATTGTAGGTGGAGACGAGTTACGGTTTGGGCGACAGgatgat > 6:130067/1‑139 (MQ=255) aGGATATCCTCCTAAAAGTGTGGGGACATGACTACTTCGGAACAGACCGGGTCGTTGATGATCTCCTCCGGAGACTGCGCCGAAAGATGCCTGCATTGAAGGTGGAGACGATTTCCGGTTTCGGCTACAGGATGATGtc > 7:25510/1‑139 (MQ=255) gATATCCTCCTAAAAGTGTGGGGACATGACTACTTCGGAACAGACCGGGTCGGGGCTGATCGCGGGCGGAGCCGGCGCAGAAAGAGGCCGGAATTGAAGGGGGAGACGATTTACGGTTTCGGCGACCGGATGCTGtcat > 8:216404/1‑139 (MQ=255) atCCTCCTAAAAGTGTGGGGACATGACTACTTCGGAACAGACCGGGTCGTTGATGATCTCGTCCGGAGACTGCGCAGAAAGATGCCTGAATTGAAGGTGGAGACGATTTACGGTTTCGGCTACAGGATGATGTcatcat > 8:117238/1‑139 (MQ=255) atCCTCCTAAAAGTGTGGGGACATGACTACTTCGGAACAGACCGGGTCGTGGATGATCTAGTCCGGAGACTGCGCAGAAATATTCCTGAATTGAAGGTGGGGCCGATTTACGGTTTCGGCAACAGGAAGATGTgaacat > 5:79130/1‑139 (MQ=255) tcctccTAAAAGTGTGGGGACATGACTACTTCGGAACAGACCGGGTCGTTGATGATCTCGTCCGGAGACTGCGCAGAAAGATGCCTGAATTGAAGGTGGAGACGATTTACGGTTTCGGCTACAGGATGATGTCATCATg < 1:177728/139‑1 (MQ=255) ctAAAAGTGTGGGGACATGACTACTTCGGAACAGACCGGGTCGTTGATGATCTCGTCCGGAGACTGCGCAGAAAGATGcc < 5:108209/80‑1 (MQ=255) ctAAAAGTGTGGGGACATGACTACTTCGGAACAGACCGGGTCGTTGATGATCTCGTCCGGAGACTGCGCAGAAAGATGcc > 6:108209/1‑80 (MQ=255) aaaaGTGTGGGGACATGACTACTTCGGAACAGACCGGGTCGTGGCTGATCTCGTCCGGAGACCGCGGAGAAAGATTCCGGAATTGAAGGTGGAGACGATTTACGGTTTCGGGGAAATGTTGTTGTCATCAAGATAAAc > 2:39590/1‑138 (MQ=255) tgtgGGGACATGACTACTTCGGAACAGACCGGGTCGTTgatgat > 2:60018/1‑44 (MQ=255) tgtgGGGACATGACTACTTCGGAACAGACCGGGTCGTTgatgat < 1:60018/44‑1 (MQ=255) tgtgGGGACATGACTACTTCGGAACAGACCGGGTCGTGGATGATCGCGTCCGGGGCCGGCGGAGAAAGATGGCGGAATTGAAGGTGGAGCCGAGTTTAGGTTTGGGCGACATGCAGAAGGGAGCATGAAAAACAAGCCg > 2:752/1‑139 (MQ=255) tgGGGACATGACTACTTCGGAACAGACCGGGTCGTTGGTGATCTCGTCCCGAGACAGCGCAGCAAGATGCCTGTATTGAAGGTGGAGCCGATTTACGGTTTGGGCGACCGGATGATTTCATCATGAAAAACAAGCCGCt > 2:59487/1‑139 (MQ=255) tgGGGACATGACTACTTCGGAACAGACCGGGTCGTTGATGATCTCGTCCGGAGACTGCGCAGAAAGATGCCTGAATTGAAGGTGGAGACGATTTACGGTTTCGGCTACAGGATGATGTCATCATGAAAAACAAGCCGCt < 7:117238/139‑1 (MQ=255) ggggACATGACTACTTCGGAACAGACCGGGTCGTTGATGATCTCGTCCGGAGACTGCGCAGAAAGATGCCTGAATTGAAGGTGGAGACGATTTACGGTTTCGGCTACAGGATGATGTCATCATGAAAAACAAGCCGCTc < 1:59487/139‑1 (MQ=255) cATGACTAGTTCGGAACCGACCGGGTGGTGGAGGATCGCGTCCGGCGACGGCGCAGAAAGATGCCTGAATTGAAGGGGGAGACGATTTACGGTTTCGGCTACAGGTTGATGTCATCATGAAAAACAAGCCGCTCGCGtt < 8:25510/139‑1 (MQ=255) aTGACTACTTCGGAACAGACCGGGTCGTTGATGATCTCGTCCGGAGACTGCGCAGAAAGATGCCTGAATTGAAGGTGGAGACGATTTACGGTTTCGGCTACAGGATGATGTCATCATGAAAAACAAGCCGCTCGCGttt < 5:69356/139‑1 (MQ=255) aTGACTACTGCGGAACAGACCGGGTCGTTGATGACCTCGTCCGGAGACTGCGCAGAAAGATGCCTGAATTGAAGGTGGAGACGATTTACGGTTTCGGCTACAGGATGATGTCATCATGAAAAACAAGCCGCTCGCGttt < 1:285295/139‑1 (MQ=255) cGGAACAGACCGGGTCGTGGTTGATCTCGTCCGGAGACTGGGCAGAAAGATGCCGGAATTGAAGGTGGTGACGATTTACGGTTTCGGCTACAGGATGTTGTCATCATGAAAAACAAGCCGCTCGCGTTTGAGAGTTggg > 3:24565/1‑139 (MQ=255) ggAACCGCCCGGGGCGTTTATGATCTCGGCCGGAGACTGCGCAGAAAGATGCCTGAATTGACGGTGGAGACGATTTACGGTTTCGGCTACAGGATGATGTCATCATGAAAAACAAGCCGCTCGCGTTTCAGATATggg < 7:53951/138‑1 (MQ=255) ggAACAGACCGGGGGGGTGATGATCCCGTCCGGCGACTGCGCACAAAGATGCCTTCATTGAAGGGGGAGACGATTTACGGTTTCGGCTACAGGATGTTGGCATCATGAAAAACAAGCCGCTCGCGTTTCAGATATggg < 8:186535/138‑1 (MQ=255) gAACAGACCGGGTCGTGGATGATCTAGTCCGGCGACTGCGCAGAAAGATGCCTGAATTGAAGGTGGAGACGATTTAAGGTTTCGGCTACAGGATGATGTGATCATGAAAAACAAGCCGCTCGCGGTTCAGATATGGGtt > 4:231288/1‑139 (MQ=255) aaCAGACCGGGTCGTTGATTATCTCGTCCGGAGACTGCGCAGAAAGATGCCTGAATTGAAGGTGGAGACGATTTACGGTTTCGGCTACAGGATGATGTCATCATGAAAAACAAGCCGCTCGCGTTTCAGATATGGGTTg < 6:79130/139‑1 (MQ=255) tCGTTGATGAGCTCGTCCGGAGACTGCGCAGAAAGATGCCTGAATTGAAGGTGGAGCCGATTTACGGTGTCGGCTACAGGATGAGGTCATCATGAAAAACAAGCCGCTCGCGTTTCAGATATGGGTTGTCATCTCCGGc < 7:216404/139‑1 (MQ=255) cGTTGATGATCTCGTCCGGAGAATGCGCAGAAAGATGCCTGAATTGAAGGTGGAGACGATTTACGTTTTCGGCTACAGGATGATGTCATCATGAAAAACAAGCCGCTCGCGTTTCAGATATGGGTTGTCATATCCGGCa < 5:263285/139‑1 (MQ=255) tgatgtTCTCGTCCGGGGACTGCGCAGAAAGATGCCTGAATTTATGGTGGAGACGATTTACGGGGTGGGCGTCAGGGTGAAGTTAGCATGAAAAAAAAGCCGCTCGCGGTTCATATTTTGGGTGTGCTATACGGCATcc > 6:263285/1‑139 (MQ=255) | AATATCATCAATTTGACGTCAAAGGAATTTGATCTGCTGCTATTATTTATCCATCATAAAGGGCATCCATACTCTCGTGAGGATATCCTCCTAAAAGTGTGGGGACATGACTACTTCGGAACAGACCGGGTCGTTGATGATCTCGTCCGGAGACTGCGCAGAAAGATGCCTGAATTGAAGGTGGAGACGATTTACGGTTTCGGCTACAGGATGATGTCATCATGAAAAACAAGCCGCTCGCGTTTCAGATATGGGTTGTCATATCCGGCATCC > CP006881/3204031‑3204303 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |