| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 238,253 | G→T | 20.2% | F27L (TTC→TTA) | U712_01105 ← | Uncharacterized protein ybfE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 238,253 | 0 | G | T | 20.2% | 60.9 / 4.0 | 37 | F27L (TTC→TTA) | U712_01105 | Uncharacterized protein ybfE |
| Reads supporting (aligned to +/- strand): ref base G (11/18); new base T (6/2); total (17/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTAGACAAGTGATGAGGACAAAGGGAAAGCATCTCTGATTTCGAGATTATTCAGAGATGCTTTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATACCGACTATGCGGGCGTTTTCGGATTTTTGTTTGAAGCGTTCAGAAATTTGAACTTTTCTTGAAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCAAAAAACATTTGTACAGCTAATTCTATTAATTCATTAAAACGAGTGCTGAAAAAATAG > CP006881/238123‑238382 | gTAGACAAGTGATGAGGACAAAGGGAAAGCATCTCTGATTTCGAGATTATTCAGAGATGCTTTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATACCGACTATGCGGGCGTTTTAGGATTTTTTTTTGAAGAGtt > 2:159451/1‑138 (MQ=255)gTAGACAAGTGATGAGGACAAAGGGAAAGCATCTCTGATTTCGAGATTATTCAGAGATGCTTTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATACCGACTATGCGGGAGTTTTCGGATTTTTGTTTGAAGCGtt > 7:72311/1‑138 (MQ=255)gTAGACAAGTGATGAGGACAAAGGGAAAGCATCTCTGATTTCGAGATTATTCAGAGATGCTTTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATACCGACTATGAGGGCGTTTTCTGATTTTTGTTTGTAGCGtt > 5:66818/1‑138 (MQ=255) gTGATGAGGACAAAGGGAAAGCATCTCTGATTTCGAGATTATTCAGAGATGCTTTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATACCGACTATGCGGGCGTTTTCGGATTTTTGTTTGAAGCGTTCAGAAAttt > 8:74176/1‑139 (MQ=255) gTGATGAGGACAAAGGGAAAGCATCTCTGATTTCGAGATTATTCAGAGATGCTTTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATACCGACTATGCGGGCGTTTTATGATTTTTTTTTTAATCCTTCACAAAttt > 4:130655/1‑139 (MQ=255) atgaGGACAAAGGGAAAGCATCTCTGATTTCGAGATTATTCAGAGATGCTTTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATACCGACTATGCGGGCGTTTTGGGATTTTTGTTTGAATCGTTCAGAAATTTGa > 1:248872/1‑138 (MQ=255) aaaGGGAAAGCATCTCTGATTTCGAGATTATTCAGAGATGCTTTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATACCGACTATGCGGGCGTTTTCGGATTTTTGTTTGAAGCGTTCAGAAATTTGAACTTTTCtt > 7:224522/1‑139 (MQ=255) aaGCATCTCTGATTTCGAGATTATTCAGAGATGCTTTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATACCGACTATGCGGGCGTTTTGCGATTTTTGTTTTAATCGTTCAGAAATATTAACTTTTCTTTAAAAtt > 8:154079/1‑138 (MQ=255) aaGCATCTCTGATTTCGAGATTATTCAGAGATGCTTTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATACCGACTATGCGGGAGTTTTCGGATTTTTTTTTTTAGCGTACAGAAATTTTAACATTTCATGaaaaag > 4:238414/1‑137 (MQ=255) aGCATCTCTGATTTCGAGATTATTCAGAGATGCTTTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATACCGACTATGCGGGCGTGTTTGGATTTTTTTTTTTAGGGGTGTGAAAATTTTACTTTTGTTGTAAATgg > 7:105837/1‑139 (MQ=255) tGATTTCGAGATTATTCAGAGATGCTTTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATACCGACTATGCGGGCGTTTTCGGATTTTTGTTTGAAGCGTTCAGAAATTTGAACTTTTCTTGAAAATGGGGaaa < 8:72311/136‑1 (MQ=255) ttCGAGATTATTCAGAGATGCTTTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATACCGACTATGCGGGCGTGTTGGGATTTTTTTTTTAAGAGTTAAGAAATTTTAAATTTTATTTTAAATTGGGAAACAaaaa > 2:205947/1‑138 (MQ=255) tCGAGATTATTCAGAGATGCTTTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATACCGACTATGCGGGCGTTTTGGGGTTTTTTTTTGTTTGGTTGAGAAATTTTTTATTTTATTTAAAATTGGGAAAAAATaaa > 2:269217/1‑138 (MQ=255) gagaTTATTCAGAGATGCTTTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATACCGACTATGCGGGCGTTTTCGGATTTTTGTTTGAAGCGTTCAGAAATTTGAACTTTTCTTGAAAATGGGGAAACAATAAAAAc < 1:205947/139‑1 (MQ=255) agagATGCTTTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATACCGACTATGCGGGCGTTTTCGGATTTTTGTTTGAAGCGTTCAGAAATTTGAACTTTTCTTGAAAATGGGGAAACAATAAAAACCCAAAAAAgg < 2:289525/139‑1 (MQ=255) cTTTTTTTGTATTTTTGTTCTAATACGAAGTCGCTCATACCGACTATGCGGGCGTGGTGGGTTGTTTGTGTGTATCGGTCAGAAATTTGAACGTTTGTTTCTAATGGGGGAACAATAAAAACCCAAAAAAAGCCGagg > 8:54688/1‑136 (MQ=255) tttGTATTTTTGTTCTAATACGAAGTCGATCATACCGACTATGCGGGCGTGTTGGGATTTTTGTTTGAAGCGTTCAGAAATTTTAACTTTTCTTGAAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGc > 5:211662/1‑138 (MQ=255) tATTTTTGTTCTAATACGACGTCGATCATACAGACTATGAGGGCGGTTTCTTATTTTTTTTTTAAGAGTTCAGAAATTTGAACTTTTCTTGAAAATGGGGAAACAATAATAAACCAATAAAGGCCGATCAAGGCaaaa < 7:274325/138‑1 (MQ=255) tATTTTTGTTCTAATACGAAGTCGATCATACCGACTATGCGGGCGTTTTCGGATTTTTGTTTGAAGCGTTCAGAAATTTGAACTTTTCTTGAAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCaaaa < 5:27840/138‑1 (MQ=255) ttCTAATACGAAGTCGATCATACCGACTATGCGGGCGTTTTCGGATTTTTGTTTGAAGCGTTCAGAAATTTGAACTTTTCTTGAAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCAAAAAACATTTGt < 2:21166/139‑1 (MQ=255) ttCTAATACGAAGTCGATCATACCGACTATGCGGGCGTTTTCGGATTTTTGTTTGAAGCGTTCAGAAATTTGAACTTTTCTTGAAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCAAAAAACATTTGt < 4:43885/139‑1 (MQ=255) cGAAGTCGATCATACCTAGTATGCGGGCGTTTTGGGATTTTTGTTTTAAGGTTTAATAAATTTGAACTTTTATTTAAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCAAAAAACATTTGTACAGCTaa < 2:269906/139‑1 (MQ=255) cGAAGTCGATCATACCGACTATGCGGGCGTTTTCGGATTTTTGTTTGAAGCGTTCAGAAATTTGAACTTTTCTTGAAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCAAAAAACATTTGTACAGCTaa < 8:173126/139‑1 (MQ=255) cGAAGTCGATCATACCGACTATGCGGGCGTTTTCGGATTTTTGTTTGAAGCGTTCAGAAATTTGAACTTTTCTTGAAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCAAAAAACATTTGTACAGCTa < 5:106129/138‑1 (MQ=255) aGTCGATCATACCGACTATGCGGGCGTTTTCGGATTTTTGTTTGAAGCGTTCAGAAATTTGAACTTTTCTTGAAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCAAAAAACATTTGTACAGCTAATTc < 7:74176/139‑1 (MQ=255) aTCATACCGACTATGCGGGCGTGTTCGGTATTTTTTTTTAAGCGGTCAGAAATTTGAAGTTTTGTTTTTAATGGTGAAAAAAAAAAAAACCCAAAAATACAGAAGAAAGCAAAAAACATTTGTTCAGCTAATTATAtt > 6:90615/1‑138 (MQ=255) tCATACCGACTATGCGGGCGTTTTCGGATTTTTGTTTGAAGCGTTCAGAAATTTGAACTTTTCTTGAAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCAAAAAACATTTGTACAGCTAATTCTATTaa < 3:112931/139‑1 (MQ=255) aTACCGACTATGCGGGCTTTTTCGGATTTTTGTTTGAAGCGTTCAGAAATTTGAACTTTTCTTGAAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCAAAAAACATTTGTACAGCTAATTCTATTAAt < 6:66818/138‑1 (MQ=255) aCCGACTATGCGGGCGTTTTCGGATTTTTGTTTGAAGCGGTCAGAAATTTTAACTTTTGTTGTAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCAAAAAACATTTTTACAGCTAATTATATTTATTCa > 5:104364/1‑139 (MQ=255) ttttCGGATTTTTTTTTGAAGCCTTAAGAAATTTTAACTTTTATTGAAAATGGGGAAACAATAAAAAACCAAAAAAGGCCCATAAAGGCAAAAAAAAATTTTACAGCTAATTCTATTAATTAATTAAAAAGAGTGCTg > 3:94103/1‑138 (MQ=255) ttttCGGATTTTTGTTTGAAGCGTTCAGAAATTTGAACTTTTCTTGAAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCAAAAAACATTTGTACAGCTAATTCTATTAATTCATTAAAACGAGTGCTGa < 3:130655/139‑1 (MQ=255) ggATTTTTGTTTGAAGCGTTCAGAAATTTGAACTTTTCTTGAAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCAAAAAACATTTGTACAGCTAATTCTATTAATTCATTAAAACGAGTGCTGaaaaaa < 2:248872/139‑1 (MQ=255) tttttGTTTGAAGCGTTCAGAAATTTGAACTTTTCTTGAAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCAAAAAACATTTGTACAGCTAATTCTATTAATTCATTAAAACGAGTGCTGAAAAAATAg < 1:159451/139‑1 (MQ=255) tttttGTTTGAAGCGTTCAGAAATTTGAACTTTTCTTGAAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCAAAAAACATTTGTACAGCTAATTCTATTAATTCATTAAAACGAGTGCTGAAAAAATAg < 3:200567/139‑1 (MQ=255) ttttttttGATGCGTTCATAAATTTGAAATTTTCTTTAAATTGGGGAAACAAAAAAAACCAAAAAAAGGCCGATAAAGGCAAAAAACATTTGTAAAGCTAATTCTATAAATTCATTAAAACGAGTGCTGAAAAAATAg < 2:140845/138‑1 (MQ=255) ttttGTTTGAAGCGTTCATAAATTTGAACTTTTCTTGAAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCAAAAAACATTTGTACAGCTAATTCTATTAATTCATTAAAACGAGTGCTGAAAAAATAg < 5:46258/138‑1 (MQ=255) ttttGTTTGAAGCGTTCAGAAATTTGAACTTTTCTTGAAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCAAAAAACATTTGTACAGCTAATTCTATTAATTCATTAAAACGAGTGCTGAAAAAATAg < 7:154079/138‑1 (MQ=255) | GTAGACAAGTGATGAGGACAAAGGGAAAGCATCTCTGATTTCGAGATTATTCAGAGATGCTTTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATACCGACTATGCGGGCGTTTTCGGATTTTTGTTTGAAGCGTTCAGAAATTTGAACTTTTCTTGAAAATGGGGAAACAATAAAAACCCAAAAAAGGCCGATCAAGGCAAAAAACATTTGTACAGCTAATTCTATTAATTCATTAAAACGAGTGCTGAAAAAATAG > CP006881/238123‑238382 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |