Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 695,557 C→A 6.5% V101F (GTT→TTT)  U712_03390 ← putative HTH‑type transcriptional regulator yerO

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP006881695,5570CA 6.5% 194.4 / 3.4 62V101F (GTT→TTT) U712_03390putative HTH‑type transcriptional regulator yerO
Reads supporting (aligned to +/- strand):  ref base C (23/35);  new base A (2/2);  total (25/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.86e-01

ATACCGCTCGATTCCTTCGCCATAGGATACCAGCAAGGCGTTCCGGTACAGCTTATCCGTCTCCATGGTTACTTTGTAAAAGTATTGCTTGATTTCTTGATTTTCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCTTTTTTAACACTTCCTTAGGCGGCTTTCCGGCGAGATCTTCTTCTATATTTTTCATTTTGTTCATAGACATACCGATATAA  >  CP006881/695423‑695687
                                                                                                                                      |                                                                                                                                  
aTACCGCTCGATTCCTTCGCCATAGGATACCAGCAAGGCGTTCCGGTACAGCTTATCCGTCTCCATGGTTACTTTGTAAAAGTATTGCTTGATTTCTTGATTTTACGGAATGATATTTTCGGTAAGCAAAAGAACGATa                                                                                                                                <  8:166065/139‑1 (MQ=255)
  aCCGCTCGATTCCTTCGCCATAGGATACCAGCAAGGCGTTCCGGTACAGCTTATCCGTCTCCATGGTTACTTTGTAAAAGTATTGCTTGATTTCTTGATTTTCCGGAATGATATTTTCGGTAAGCAAAAGAACGATaa                                                                                                                               >  5:112059/1‑138 (MQ=255)
      cTCGATTCCTTCGCCATAGGATACCAGCAAGGCGTTCCGGTACAGCTTATCCGTCTCCATGGTTACTTTGTAAAAGTATTGCTTGATTTCTTGATTTTCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCt                                                                                                                          >  2:281606/1‑139 (MQ=255)
      cTCGATTCCTTCGCCATAGGATACCAGCAAGGCGTTCCGGTACAGCTTATCCGTCTCCATGGTTACTTTGTAAAAGTATTGCTTGATTTCTTGATTTTCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCt                                                                                                                          >  8:101134/1‑139 (MQ=255)
      cTCGATTCCTTCGCCATAGGATACCAGCAAGGCGTTCCGGTACAGCTTATCCGTCGCCATGGTTACTTTGTAAAAGTATTGCTTGATTTCTTGATTTTCCGGCATGATATTTTCGGTAAGCAAAAGAACGATAAAATCt                                                                                                                          >  4:199010/1‑139 (MQ=255)
         gATTCCTTCGCCATAGGATACCAGCAAGGCGTTCCGGTACAGCTTATCCGTCTCCATGGTTACTTTGTAAAAGTATTGCTTGATTTCTTGATTTTCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTa                                                                                                                       >  4:255722/1‑139 (MQ=255)
                 cGCCATAGGATACCAGCAAGGCGTTCCGGTACAGCTTATCCGTCTCCATGGTTACTTTGTAAAAGTATTGCTTGATTTCTTGATTTTCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATcgc                                                                                                                >  3:225665/1‑138 (MQ=255)
                 cGCCATAGGATACCAGCAAGGCGTTCCGGGACAGCTTATCCGTCTCCATGGTTACTTTGTAAAAGTATTGCTTGATTTCTTGATTTTCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATcg                                                                                                                 <  4:138633/137‑1 (MQ=255)
                   ccATAGGATACCAGCAAGGCGTTCCGGTACAGCTTATCCGTCTCCATGGTTACTTTGTAAAAGTATTGCTTGATTTTTTGATTTTCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGaa                                                                                                             <  3:189478/139‑1 (MQ=255)
                   ccATAGGATACCAGCAAGGCGTTCCGGTACAGCTTATCCGTCTCCATGGTTACTTTGTAAAAGTATTGCGTGATTTCTTGATTTTCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGaa                                                                                                             <  1:104941/139‑1 (MQ=255)
                     aTAGGATACCAGCAAGGCGTTCCGGTACAGCTTATCCGTCTCCATGGTTACTTTGTAAAAGTATTGCTTGATTTCTTGATTTTCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGaaaa                                                                                                           <  4:225665/139‑1 (MQ=255)
                                   aGGCGTTCCGGTACAGCTTATCCGTCTCCATGGTTACTTTGTAAAAGTATTGCTTGATTTCTTGATTTTCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAgc                                                                                             >  6:9775/1‑139 (MQ=255)
                                    ggCGTTCCGGTACAGCTTATCCGTCTCCATGGTTACTTTGTAAAAGTATTGCTTGATTTCTTGATTTTCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAgcg                                                                                            >  6:265666/1‑139 (MQ=255)
                                        ttCCGGTACAGCTTATCCGTCTCCATGGTTACTTTGTAAAAGTATTGCTTGATTTCTTGATTTTCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCaa                                                                                        >  4:93513/1‑139 (MQ=255)
                                        ttCCGGTACAGCTTATCCGTCTCCATGGTTACTTTGTAAAAGTATTGCTTGATTTCTTGATTTTCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCaa                                                                                        >  4:289367/1‑139 (MQ=255)
                                             gTACAGCTTATCCGTCTCCATGGTTACTTTGTAAAAGTATTGCTTGATTTCTTGATTTTCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGc                                                                                   <  3:201164/139‑1 (MQ=255)
                                                   cTTATCCGTCTCCATGGTTACTTTGTAAAAGTATTGCTTGATTTCTTGATTTTCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCttttt                                                                              >  8:145531/1‑138 (MQ=255)
                                                   cTTATCCGTCTCCATGGTTACTTTGTAAAAGTATTGCTTGATTTCTTGATTTTCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGAGCCAATTTGCttttt                                                                              >  6:46324/1‑138 (MQ=255)
                                                   cTTATCCGTCTCCATGGTTACTTTGTAAAAGTATTGCTTGATTTCTTGATTTTCCGGAATGATATTTTCGGTAAGCAAAAGAAAGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCttttt                                                                              >  8:169462/1‑138 (MQ=255)
                                                   cTTATCCGTCTCCATGGTTACTTTGTAAAAGTATTGCTAGATTTCTTGATTTTCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTTTGATCTCGAAAATCCTCAAATAGAGCGCCATTTTGCttttt                                                                              >  3:48469/1‑138 (MQ=255)
                                                   cNTATCCGTCNCCATNGTTACTTTGTAAAAGTATTGCTTGATTTCTTGATTTTCCGGAATGATATTTTCGGTAAGCAAAAGAAAGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCttttt                                                                              >  8:173634/1‑138 (MQ=255)
                                                     tATCCGTCTCCATGGTTACTTTGTAAAAGTATTGCTTGATTTCTTGATTTTCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCTTTTTTaa                                                                           >  6:162197/1‑139 (MQ=255)
                                                       tCCGTCTCCATGGTTACTTTGTAAAAGTATTGCTTGATTTCTTGATTTTCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCTTTTTTAaca                                                                         <  7:283575/139‑1 (MQ=255)
                                                       tCCGTCTCCATGGTTACTTTGTAAAAGTATTGCTTGATTTCTTGATTTTCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCTTTTTTAaca                                                                         <  1:281606/139‑1 (MQ=255)
                                                       tCCGTCTCCATGGTTACTTTGTAAAAGTATTGCTTGATTTCTTGATTTTCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCTTTTTTAaca                                                                         <  7:145531/139‑1 (MQ=255)
                                                       tCCGTCTCCATGGTTACTTTGTAAAAGTATTGCTTGATTTCTTGATTTTCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCTTTTTTAaca                                                                         <  1:56735/139‑1 (MQ=255)
                                                           tctcCATGGTTACTTTGTAAAAGTATTGCTTGATTTCTTGATTTTCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCTTTTTTAaca                                                                         <  1:130712/135‑1 (MQ=255)
                                                           tctcCATGGTTACTTTGTAAAAGTATTGCTTGATTTCTTGATTTTCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCTTTTTTAaca                                                                         >  2:130712/1‑135 (MQ=255)
                                                           tctcCATGGTTACTTTGTAAAAGTATTGCTTGATTTCTTGATTTTCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCTTTTTTAACACTTc                                                                     >  2:69524/1‑139 (MQ=255)
                                                             tcCATGGTTACTTTGTAAAAGTATTGCTTGATTTCTTGATTTTCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGaa                                                                                                             <  4:225016/97‑1 (MQ=255)
                                                             tcCATGGTTACTTTGTAAAAGTATTGCTTGATTTCTTGATTTTCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGaa                                                                                                             >  3:225016/1‑97 (MQ=255)
                                                             tcCATGGTTACTTTGTAAAAGTATTGCTTGATTTCTTGATTTTCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCTTTTTTAACACTTCCt                                                                   >  5:188284/1‑139 (MQ=255)
                                                                            tAAAAGTATTGCTTGATTTCTTGATTTTCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCTTTTTTAACACTTCCTTAGGCGGCTTTCCgg                                                    >  1:206365/1‑139 (MQ=255)
                                                                            tAAAAGTATTGCTTGATTTCTTGATTTTCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCTTTTTTAACACTTCCTTAGGCGGCTTTCCgg                                                    >  2:239686/1‑139 (MQ=255)
                                                                            tAAAAGTATTGCTTGATTTCTTGATTTTCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCTTTTTTAACACTTCCTTAGGCGGCTTTCCgg                                                    >  6:177583/1‑139 (MQ=255)
                                                                                 gTATTGCTTGATTTCTTGATTTTCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCTTTTTTAACACTTCCTTAGGCGGCTTTCCGGc                                                   >  8:30468/1‑135 (MQ=255)
                                                                                    ttGCTTGATTTCTTGATTTTCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCTTTTTTAACACTTCCTTAGGCGGCTTTCCGGCGAGAtct                                            >  2:246152/1‑139 (MQ=255)
                                                                                    ttGCTTGATTTCTTGATTTTCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCTTTTTTAACACTTCCTTAGGCGGCTTTCCGGCGAGAtc                                             <  7:175965/138‑1 (MQ=255)
                                                                                    ttGCTTGATTTCTTGATTTTCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCTTTTTTAACACTTCCTTAGGCGGCTTTCCGGCGAGAtc                                             <  8:158663/138‑1 (MQ=255)
                                                                                    ctGCTTGATTTCTTGATTTTCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCTTTTTTAACACTTCCTTAGGCGGCTTTCCGGCGAGAtc                                             <  3:166706/137‑1 (MQ=255)
                                                                                      gCTTGATTTCTTGATTTTCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCTTTTTTAACACTTCCTTAGGCGGCTTTCCGGCGAGAtctt                                           <  1:69524/138‑1 (MQ=255)
                                                                                            tttcttgatttTCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCTCCAATTTGCTTTATTAACACTTCATTAGGCGGCTTTCCGGCGATATCTACTTCtat                                    <  6:274355/139‑1 (MQ=255)
                                                                                                       tCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCTTTTTTAACACTTCCTTAGGCGGCTTTCCGGCGAGATCTTCTTCTATATTTTTCAtt                          <  3:93513/138‑1 (MQ=255)
                                                                                                       tCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCTTTTTTAACACTTCCTTAGGCGGCTTTCCGGCGAGATCTTCTTCTATATTTTTCAtt                          <  5:177583/138‑1 (MQ=255)
                                                                                                       tCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCTTTTTTAACACTTCCTTAGGCGGCTTTCCGGCGAGATCTTCTTCTATATTTTTCAtt                          <  7:30468/138‑1 (MQ=255)
                                                                                                       tCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCTTTTTTAACACTTCCTTAGGCGGCTTTCCGGCGAGATCTTCTTCTATATTTTTCAtt                          <  6:26261/138‑1 (MQ=255)
                                                                                                       tCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCTTTTTTAACACTTCCTTAGGCGGCTTTCCGGCGAGATCTTCTTCTATATTTTTCAtt                          <  6:188284/138‑1 (MQ=255)
                                                                                                       tCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCTTTTTTAACACTTCCTTAGGCGGCTTTCCGGCGAGATCTTCTTCTATATTTTTCAtt                          <  1:239686/138‑1 (MQ=255)
                                                                                                       tCCGGAATGATATTTTCGGTAAGCAAAAGAAAGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCTTTTTTAACNCTTCCTTAGGCGGCTTTNCGGNGAGATCTNCTTCTATATTTTTCAtt                          <  7:173634/138‑1 (MQ=255)
                                                                                                       tCCGGAATGATATTTTCGGTAAGCAAAAGAAAGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCTTTTTTAACACTTCCTTAGGCGGCTTTCCGGCGAGATCTTCTTCTATATTTTTCAtt                          <  7:169462/138‑1 (MQ=255)
                                                                                                         cGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCTTTTTTAACACTTCCTTAGGCGGCTTTCCGGCGAGATCTTCTTCTATATTTTTCATTTTg                       <  3:255722/139‑1 (MQ=255)
                                                                                                           gAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCTTTTTTAACACTTCCTTAGGCGGCTTTCCGGCGAGATCTTCTTCTATATTTTTCATTTTGtt                     <  3:289367/139‑1 (MQ=255)
                                                                                                           gAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCTTTTTTAACACTTCCTTAGGCGGCTTTCCGGCGAGATCTTCTTCTATATTTTTCATTTTGtt                     <  2:206365/139‑1 (MQ=255)
                                                                                                           gAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCTTTTTTAACACTTCCTTAGGCGGCTTTCCGGCGAGATCTTCTTCTATATTTTTCATTTTGtt                     <  3:264397/139‑1 (MQ=255)
                                                                                                           gAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCTTTTTTAACACTTCCTTAGGCGGCTTTCCGGCGAGATCTTCTTCTATATTTTTCATTTTGtt                     <  5:9775/139‑1 (MQ=255)
                                                                                                                atatTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCTTTTTTAACACTTCCTTAGGCGGCTTTCCGGCGAGATCTTCTTCTATATTTTTCATTTTGTTCATa                 <  1:283840/138‑1 (MQ=255)
                                                                                                                   ttttCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCTTTTTTAACACTTCCTTAGGCGGCTTTCCGGCGAGATCTTCTTCTATATTTTTCATTTTGTTCATAGACa             <  7:101134/139‑1 (MQ=255)
                                                                                                                   ttttCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCTTTTTTAACACTTCCTTAGGCGGCTTTCCGGCGAGATCTTCTTCTATATTTTTCATTTTGTTCATAGACa             <  6:112059/139‑1 (MQ=255)
                                                                                                                   ttttCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCTTTTTTAACACTTCCTTAGGCGGCTTTCCGGCGAGATCTTCTTCTATATTTTTCATTTTGTTCATAGACa             <  4:152042/139‑1 (MQ=255)
                                                                                                                   ttttCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCTTTTTTAACACTTCCTTAGGCGGCTTTCCGGCGAGATCTTCTTCTATATTTTTCATTTTGTTCATAGACa             <  3:197864/139‑1 (MQ=255)
                                                                                                                        ggTAAGCAAAAGAACGCTAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCTTTTTTAACACTTCCTTAGGCGGCTTTCCGGCGAGATCTTCTTCTATATTTTTCATTTTGTTCATAGACATACCg        <  3:199010/139‑1 (MQ=255)
                                                                                                                              cAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCTTTTTTAACACTTCCTTAGGCGGCTTTCCGGCGAGATCTTCTTCTATATTTTTCATTTTGTTCATAGACATACCGATataa  <  1:246152/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                  
ATACCGCTCGATTCCTTCGCCATAGGATACCAGCAAGGCGTTCCGGTACAGCTTATCCGTCTCCATGGTTACTTTGTAAAAGTATTGCTTGATTTCTTGATTTTCCGGAATGATATTTTCGGTAAGCAAAAGAACGATAAAATCTTTATGATCGCGAAAATCCTCAAATTGAGCGCCAATTTGCTTTTTTAACACTTCCTTAGGCGGCTTTCCGGCGAGATCTTCTTCTATATTTTTCATTTTGTTCATAGACATACCGATATAA  >  CP006881/695423‑695687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑