Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,724,719 G→A D36D (GAC→GAT)  U712_19200 ← SacPA operon antiterminator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,724,7190GA98.1% 354.7 / NA 107D36D (GAC→GAT) U712_19200SacPA operon antiterminator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (58/47);  minor base T (0/2);  total (58/49)
Fisher's exact test for biased strand distribution p-value = 2.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

CCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCAATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTAAACTTTTCATTCTCGTCGTGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGGTCATTTTTCTTTTTTTGAAAAGCGATTCCTGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAGTACTTTATAGATTTTCAAGAGGCTATCTCTCCCGCCAATTAGCTT  >  CP006881/3724587‑3724853
                                                                                                                                    |                                                                                                                                      
tccTTCCGCATAGCTGATGATAACCTCAGCAATTTCTATATGCTCCTCCGGCAGTGTTTGCACGATTTGTTTGAACTTTTCATTCTCGTAGCGCACCACAAACATCTTTTACACTTTAATCATAAGGATGAGATcctgt                                                                                                                                  >  2:223026/2‑135 (MQ=255)
        cATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCttttt                                                                                                                          >  8:199689/1‑139 (MQ=255)
        cATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCttttt                                                                                                                          >  3:312927/1‑139 (MQ=255)
          tAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCttttttt                                                                                                                        >  4:153362/1‑139 (MQ=255)
          tAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCAATTAATTCATAGGGATGAGATCATTTTTCttttttt                                                                                                                        >  1:445594/1‑139 (MQ=255)
             cTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGATATCATTTTTCTTTTTTTGa                                                                                                                      <  8:179901/138‑1 (MQ=255)
               gatgatATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGaa                                                                                                                     >  2:55211/1‑137 (MQ=255)
                atgatATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTGTTTTTTTGAAAAg                                                                                                                  >  1:361036/1‑139 (MQ=255)
                atgatATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAg                                                                                                                  >  3:382203/1‑139 (MQ=255)
                atgatATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTAATAGGGATGAGATCATTTTTCTTTTTTTGAAAAg                                                                                                                  >  5:303391/1‑139 (MQ=255)
                  gatATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCGGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGc                                                                                                                 >  7:326279/1‑138 (MQ=255)
                  gatATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGc                                                                                                                 >  3:192280/1‑138 (MQ=255)
                     atCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGTTGAGATCATTTTTCTTTTTTTGAAAATCGAtt                                                                                                             >  6:322886/1‑139 (MQ=255)
                       ccTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTcc                                                                                                           >  8:400547/1‑139 (MQ=255)
                       ccTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTcc                                                                                                           >  5:36680/1‑139 (MQ=255)
                        cTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTcc                                                                                                           <  6:429713/138‑1 (MQ=255)
                          cAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCgg                                                                                                        >  6:480801/1‑139 (MQ=255)
                           aGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAATAGCGATTccctgg                                                                                                       >  2:379312/1‑135 (MQ=255)
                           aGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTcccgtt                                                                                                       >  8:110879/1‑135 (MQ=255)
                           aGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGt                                                                                                       >  5:372316/1‑139 (MQ=255)
                             cTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTcc                                                                                                     >  1:125266/1‑139 (MQ=255)
                                   ctatatGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCa                                                                                                <  3:184737/136‑1 (MQ=255)
                                       atGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGAtt                                                                                           >  7:320923/1‑139 (MQ=255)
                                          ctcctcCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGAttttt                                                                                        >  2:260523/1‑139 (MQ=255)
                                             ctccGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTcc                                                                                     >  4:186309/1‑139 (MQ=255)
                                             ctccGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTcc                                                                                     >  4:166353/1‑139 (MQ=255)
                                             ctccGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTcc                                                                                     >  8:379659/1‑139 (MQ=255)
                                             ctccGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTcc                                                                                     >  6:247316/1‑139 (MQ=255)
                                              tccGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCt                                                                                    >  2:40271/1‑139 (MQ=255)
                                              tccGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGAAAAAGAACATATCCACTGTAATCATAGGGAGGAGAACAATTTTCATTTTTTGAAAAGCGATACCCGGTCCCCACCCGATATTTTcc                                                                                     >  6:329142/1‑138 (MQ=255)
                                                    aGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGatcat                                                                              <  5:54082/139‑1 (MQ=255)
                                                     gtgtTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGatcatc                                                                             <  7:407621/139‑1 (MQ=255)
                                                     gtgtTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGatcatc                                                                             <  8:121534/139‑1 (MQ=255)
                                                       gtTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGCTCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGGCCCAGCACGATTTTTTCCTGATGATGct                                                                           >  5:121585/1‑139 (MQ=255)
                                                          tGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCtt                                                                        >  5:472551/1‑139 (MQ=255)
                                                                aTTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTttatt                                                                   >  2:228963/1‑138 (MQ=255)
                                                                aTTTGTTTGAACTTTTCATTCTCGTCGCGCAAGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTttatta                                                                  <  6:27811/139‑1 (MQ=255)
                                                                 tttgtttgAACTTTTCATTATCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTaa                                                                 <  3:153362/139‑1 (MQ=255)
                                                                    gTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAg                                                               <  3:186309/138‑1 (MQ=255)
                                                                    gTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGc                                                              >  7:342430/1‑139 (MQ=255)
                                                                       tgaaCTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCg                                                            <  6:160303/136‑1 (MQ=255)
                                                                         taCTTTTCATTCTCCTCGCGCACGACAAAGATCTTTTACAATTTTATCATAGGGATGAGATCATTTTTATTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCa                                                          <  4:87957/137‑1 (MQ=255)
                                                                           cTTTTCATTCTCGTCGCGCACTACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCAttg                                                       <  5:16309/139‑1 (MQ=255)
                                                                           cTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCAttg                                                       <  6:372316/139‑1 (MQ=255)
                                                                           cTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCAttg                                                       <  4:179305/139‑1 (MQ=255)
                                                                           cTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCAttg                                                       <  7:400547/139‑1 (MQ=255)
                                                                           cTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCAttg                                                       <  7:379659/139‑1 (MQ=255)
                                                                           cTTTTCATTCTCGTCGCGAACGACAAAGATCTTTTCCACTTTATTCATCGGGATGAGTTCATTTTTCTTTTTTTGAAATGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCAttg                                                       <  6:64405/139‑1 (MQ=255)
                                                                           cTTTTCATTCTCGTAGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCAttg                                                       <  7:453496/139‑1 (MQ=255)
                                                                           cTTTTAATTCTCGTCGCTCACGACAAAGATCTTTTCCACTTTATTCATAGGGTTGATATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCAttg                                                       <  2:445594/139‑1 (MQ=255)
                                                                           attttCATTATCGCCCCGCACCACAAATATCTTTTCCACTTTATTCATAGGGATGACATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCAttg                                                       <  2:433160/138‑1 (MQ=255)
                                                                              ttCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCGGGTCCCATCCCGATTTTTTCCGGATCATCCTCTTTTATTAAAGACGCAttgttg                                                    >  6:389385/1‑139 (MQ=255)
                                                                              ttCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCAttgttg                                                    >  1:433160/1‑139 (MQ=255)
                                                                               tCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATtgttgt                                                   >  1:198142/1‑139 (MQ=255)
                                                                                 aTTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTgttgtt                                                  <  1:324771/138‑1 (MQ=255)
                                                                                  ttCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTa                                                <  4:220705/139‑1 (MQ=255)
                                                                                  ttCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTa                                                <  3:166353/139‑1 (MQ=255)
                                                                                  ttCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTa                                                <  4:163443/139‑1 (MQ=255)
                                                                                    ctcGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATTAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTaa                                               <  5:480801/138‑2 (MQ=255)
                                                                                    ctcGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTaa                                               <  3:409344/138‑2 (MQ=255)
                                                                                    ctcGGCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTaa                                               <  2:459213/138‑2 (MQ=255)
                                                                                      cgtcgcGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTa                                                <  3:386602/135‑1 (MQ=255)
                                                                                      cgtcgcGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTa                                                >  4:386602/1‑135 (MQ=255)
                                                                                           cgCACGACATAGATCTTTTCCACTTTATTCATAGGGATGAGATGATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTtat                                       <  7:107932/138‑1 (MQ=255)
                                                                                           cgCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTtat                                       <  5:201921/138‑1 (MQ=255)
                                                                                           cgCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTtat                                       <  8:329217/138‑1 (MQ=255)
                                                                                             cACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCTATTCCCGGTCCCATCACGATTTTTTCCTGatcatc                                                                             >  2:48343/1‑99 (MQ=255)
                                                                                             cACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGatcatc                                                                             <  1:48343/99‑1 (MQ=255)
                                                                                              aCGACAAAGATCTTTTCCACTTTATTCATAGGTATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATGACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGa                                    <  1:55211/139‑1 (MQ=255)
                                                                                              aCGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGa                                    <  4:26115/139‑1 (MQ=255)
                                                                                                     tgATCTTTAACACTTTATTCTTAGGGATGCCTTCATTTTTCTTTTTTTCAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCaa                             <  6:323394/138‑1 (MQ=255)
                                                                                                     ataTCTTTTACACTTTATGCATAGGGATTAGATCAGTTTTATTTTTTTGAAAAGCGATTCCCGTTCCCATCACGATTTTTTCCTGATAATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCaa                             <  7:302460/137‑1 (MQ=255)
                                                                                                     agttCTTTACAACTTTTTTAATTGGGTTGTGATGATTTTTCTTTTTTTTAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTTAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCaa                             <  5:329142/136‑1 (MQ=255)
                                                                                                     aGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCaa                             <  7:227052/139‑1 (MQ=255)
                                                                                                     aGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGAATCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCaa                             <  6:319295/139‑1 (MQ=255)
                                                                                                          ttttCCACTTTATTCATAGGGATGAGATCATTTTTATTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTGCTGATCATCCTCTTTTATTAAAGACGAATAGTTGTTTAATACTTTATAGATTTTCAagatgt                        >  4:450862/1‑136 (MQ=255)
                                                                                                            ttacACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGATTAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCGTCTTTTATTAAAGCAGCTTTGTTGTTTAGTACTTTATAGATTTTCAAGAGGTTa                      >  8:203642/4‑139 (MQ=255)
                                                                                                            ttCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTa                                                >  7:135822/1‑113 (MQ=255)
                                                                                                            ttCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTa                                                <  8:135822/113‑1 (MQ=255)
                                                                                                            ttCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATATAtt                                  >  5:278844/1‑127 (MQ=255)
                                                                                                            ttCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGAtt                                  <  6:278844/127‑1 (MQ=255)
                                                                                                            ttCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGACGCATTGTTGTTTAATACTTTATAGATTTTCa                              >  3:240294/1‑131 (MQ=255)
                                                                                                            ttCCACTTTATTCATAGGGATGAGATCATTTTTCTTATTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCATCTTTTATTAAAGCAGCATTGTTTTTTAATACTTTATAGATTTTCAAGAGGTTa                      >  5:169587/1‑139 (MQ=255)
                                                                                                                 cTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAATCCGCATTGTTGTTTAATACTTTATAGATTTTCaa                             >  7:342010/1‑127 (MQ=255)
                                                                                                                 cTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCaa                             <  8:342010/127‑1 (MQ=255)
                                                                                                                 cTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTAtctct                 >  3:152602/1‑139 (MQ=255)
                                                                                                                   ttATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTc                               <  1:294323/123‑1 (MQ=255)
                                                                                                                   ttATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTcc               >  4:296563/1‑139 (MQ=255)
                                                                                                                         aTAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCaa         >  2:95155/1‑139 (MQ=255)
                                                                                                                           aGGTATGAGATATTTTTTATTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAAt        <  2:217178/138‑1 (MQ=255)
                                                                                                                           aGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTa                                                <  4:461167/98‑1 (MQ=255)
                                                                                                                           aGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATAATCCTCTTTTATTAAAGCCGCATTGTTGTTTa                                                >  3:461167/1‑98 (MQ=255)
                                                                                                                            gggATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCctct                                                                         >  4:341615/1‑72 (MQ=255)
                                                                                                                            gggATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCctct                                                                         <  3:341615/72‑1 (MQ=255)
                                                                                                                              gATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGc    >  6:214227/1‑139 (MQ=255)
                                                                                                                                tGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCAttg                                                       >  3:311594/1‑86 (MQ=255)
                                                                                                                                tGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCAttg                                                       <  4:311594/86‑1 (MQ=255)
                                                                                                                                tGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCtt  <  5:247316/139‑1 (MQ=255)
                                                                                                                                tGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCtt  >  1:36373/1‑139 (MQ=255)
                                                                                                                                tGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCtt  >  1:217178/1‑139 (MQ=255)
                                                                                                                                    |                                                                                                                                      
CCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCAATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTAAACTTTTCATTCTCGTCGTGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGGTCATTTTTCTTTTTTTGAAAAGCGATTCCTGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAGTACTTTATAGATTTTCAAGAGGCTATCTCTCCCGCCAATTAGCTT  >  CP006881/3724587‑3724853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑